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Pharmaceutical composition for preventing or treating hearing impairment containing cilostazol and ginkgo biloba extract as active ingredients

A technology for cilostazol and hearing impairment, applied in the field of pharmaceutical compositions for preventing or treating hearing impairment, to achieve the effect of treating noise-induced hearing impairment or ototoxic hearing impairment and inhibiting apoptosis

Active Publication Date: 2017-06-23
SK CHEM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in order to prevent ototoxic hearing impairment, sulfhydryl drugs such as sodium thiosulfate; calcium channel blockers such as N-acetylcysteine, D- and L-methionine, and flunarizin are being studied; Aspartase (caspase) inhibitors, etc., but the fact is that there are no practical drugs

Method used

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  • Pharmaceutical composition for preventing or treating hearing impairment containing cilostazol and ginkgo biloba extract as active ingredients
  • Pharmaceutical composition for preventing or treating hearing impairment containing cilostazol and ginkgo biloba extract as active ingredients
  • Pharmaceutical composition for preventing or treating hearing impairment containing cilostazol and ginkgo biloba extract as active ingredients

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1: According to the effect analysis of compound preparation of the present invention on hearing function and vestibular function

[0056] Preparation of compound preparation

[0057] Cilostazol (CS, SK Chemicals) and Ginkgo biloba extract (GbE, SK Chemicals) were mixed in a weight ratio of 5:4 and dissolved in 0.5% (w / v) carboxymethylcellulose sodium (CMCNa, Sigma) in an aqueous solution to prepare a composite preparation (hereinafter referred to as renexin) at a concentration of 18 mg / mL.

[0058] Application of sample

[0059] 52 SD (Sprague-Dawley) rats (200-250 g) aged 8-10 weeks were divided into the following 6 groups, and each drug was administered as follows.

[0060] -The first group: normal control group (n=3);

[0061] -The second group: cisplatin (CDDP) administration group alone (n=13);

[0062] -The third group: ranashin+CDDP administration group (n=12);

[0063] - The fourth group: Ginkgo biloba extract+CDDP administration group (n=12); ...

Embodiment 2

[0080] Example 2: Morphological evaluation of auditory and vestibular hair cells administered according to the compound formulation of the present invention

[0081] Morphological evaluation of the middle and bottom

[0082] The rats in each group of Example were anesthetized, and then their temporal bones were extracted. Under a dissecting microscope, in the cochlea The round window (round window) and oval window (oval window) were drilled, and the perilymphatic space (perilymphatic space) was perfused with 4% glutaraldehyde (EMgrade, place of origin, Inc.CA, USA) with a Pasteur pipette. Each sample was then fixed in 4% glutaraldehyde solution for 24 hours. The surface of the cochlea and the perilymphatic space were washed three times with phosphate buffered solution (PBS), perfused with 1% osmium tetroxide, and placed on a tissue rotator for 15 minutes. Then, the sample was washed 3 times with PBS, the bony capsule of the cochlea was removed under a dissecting microsco...

Embodiment 3

[0094] Embodiment 3: Analysis of the in vitro effect of the compound preparation of the present invention on CDDP-induced cytotoxicity

[0095] Confirmation of CDDP-induced cytotoxicity

[0096] In order to confirm CDDP-induced cytotoxicity, the auditory cell line (HEI-OC1 (ATCC, USA) was cultured at 33°C with 10% CO2 in Dulbecco's essential medium (DMEM, Dulbecco's minimum essential medium) was cultured. The cultured cells were divided into 96-well plates at a concentration of 3000 cells / well, and then 20 μM cisplatin was added for culture. At 0, 12, 24 and 48 hours of culture , add 20 μl of MTS solution to each well, continue to incubate for 4 hours, and then measure the absorbance of formazan at 490 nm with a fully automatic quantitative drawing microplate reader, so as to determine the proportion of surviving cells.

[0097]The measurement results are shown in Figure 8 . Such as Figure 8 As shown, according to the treatment with cisplatin, a time-dependent decrease ...

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Abstract

The present invention relates to a pharmaceutical composition for preventing or treating hearing impairment containing cilostazol and ginkgo biloba extract as active ingredients, the pharmaceutical composition inhibits the apoptosis of auditory cells caused by the generation of reactive oxygen species (ROS), Thereby, noise-induced hearing impairment or ototoxic hearing impairment can be effectively prevented or treated.

Description

technical field [0001] The invention relates to a pharmaceutical composition for preventing or treating hearing impairment containing cilostazol and ginkgo leaf extract as active ingredients. Background technique [0002] Hearing impairment is a hearing abnormality caused by various reasons. It refers to the state of hearing loss or loss. The ear is composed of the outer ear (auricle to tympanic membrane), middle ear (tympanic membrane to cochlear entrance) and inner ear (inside the cochlea). As long as there is a problem in one place, it will cause hearing loss. Hearing impairment is divided into hearing impairment caused by damage to the outer ear and middle ear (conductive hearing impairment) and auditory nerve impairment caused by damage to the inner ear and auditory nervous system (sensorineural hearing impairment). Hearing impairment can be restored by treating the above-mentioned damages, but the hearing impairment caused by damage to the inner ear and the auditory n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/4709A61K36/16A61P27/16
CPCA61K31/4709A61K36/16A61P27/16A61K2300/00
Inventor 柳根镐韩蕙英李素英
Owner SK CHEM CO LTD
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