A kind of blood green regeneration and rapid propagation method

Abstract

The invention belongs to a rapid propagation method for an important plant material in endangered wild plants and particularly relates to a regenerating and rapid propagating method for liparis nervosa. The method comprises the steps of material selection and sterilization; inducing culture; multiplication culture; inducing rooting; and transplanting. The regenerating and rapid propagating method aims at solving the problems of low seed germination rate, rooting difficulty and low growth speed in a natural environment because of having no vegetative masses in the prior art, and provides the technical support for large-scale industrial production. By using the regenerating and rapid propagating method, the expanding propagation coefficient is high, the propagation time is short, the growth processes are greatly shortened, seedlings can be obtained after carrying out inducing rooting for 20-25 days, and the survival rate of transplanting is up to more than 96%; and the cost is greatly reduced, and the seedlings are high in survival rate after being transplanted and are robust and straight, good in growth vigor, neat and consistent. The technical support is provided for protecting liparis nervosa and large-scale industrial production, popularization and application.

Description

technical field

[0001] The invention belongs to a method for rapid propagation of important plant materials in endangered wild plants, in particular to a method for regeneration and rapid propagation of Hematocele. Background technique

[0002] See Xueqing (Liparis nervosa (Thunb.) Lindl.), also known as Pulse Sheep Ear Garlic, aliased as Pleurotus nervosa, Pulse Sheep Ear Orchid, Orchidaceae (Orchidaceae) Sheep Ear Garlic (Liparis) 1 to 2-year-old herb, Jianxueqing is bitter in taste and cool in nature. It is used for hemoptysis due to lung heat, hematemesis, blood under intestinal wind, metrorrhagia, cough due to lung heat, convulsions, etc., and externally used for bleeding from planing wounds, bruises, dermatitis, sores, furuncles, and swelling. Snake venom bite. Jianxueqing polysaccharides and total alkaloids have good antioxidant and antibacterial effects, not only inhibiting bacteria, but also having strong inhibitory effects on fungi. The whole herb contains nervos...

Examples

Embodiment 1

[0026] A kind of blood green regeneration and rapid propagation method, concrete steps are as follows

[0027] 1) Material selection and disinfection: 1. Cut out 3~10cm new shoots from the base of the blood green, wrap them with wet gauze, and wash the soil on the plants with running water after taking them back to the laboratory; 2. Disinfection of plants: use After cutting off the upper leaves of the plant with medical scissors, soak in saturated bleach supernatant for 10 minutes, and lightly brush and rinse with a soft brush. Disinfect with 75% alcohol for 30 seconds, pour off the alcohol, and add 0.1% mercury liter (HgCl 2 ) for 10 minutes, pour the mercury solution into a waste mercury bottle, rinse with sterile water three times, and dry the surface with sterile filter paper before inoculation.

[0028] 2) Induction culture: cut the sterilized materials into 1 cm long stems with one node and inoculate them in the bud induction medium sterilized by high temperature and h...

technical field

[0001] The invention belongs to a method for rapid propagation of important plant materials in endangered wild plants, in particular to a method for regeneration and rapid propagation of Hematocele. Background technique

[0002] See Xueqing (Liparis nervosa (Thunb.) Lindl.), also known as Pulse Sheep Ear Garlic, aliased as Pleurotus nervosa, Pulse Sheep Ear Orchid, Orchidaceae (Orchidaceae) Sheep Ear Garlic (Liparis) 1 to 2-year-old herb, Jianxueqing is bitter in taste and cool in nature. It is used for hemoptysis due to lung heat, hematemesis, blood under intestinal wind, metrorrhagia, cough due to lung heat, convulsions, etc., and externally used for bleeding from planing wounds, bruises, dermatitis, sores, furuncles, and swelling. Snake venom bite. Jianxueqing polysaccharides and total alkaloids have good antioxidant and antibacterial effects, not only inhibiting bacteria, but also having strong inhibitory effects on fungi. The whole herb contains nervos...

Examples

Embodiment 1

[0026] A kind of blood green regeneration and rapid propagation method, concrete steps are as follows

[0027] 1) Material selection and disinfection: 1. Cut out 3~10cm new shoots from the base of the blood green, wrap them with wet gauze, and wash the soil on the plants with running water after taking them back to the laboratory; 2. Disinfection of plants: use After cutting off the upper leaves of the plant with medical scissors, soak in saturated bleach supernatant for 10 minutes, and lightly brush and rinse with a soft brush. Disinfect with 75% alcohol for 30 seconds, pour off the alcohol, and add 0.1% mercury liter (HgCl 2 ) for 10 minutes, pour the mercury solution into a waste mercury bottle, rinse with sterile water three times, and dry the surface with sterile filter paper before inoculation.

[0028] 2) Induction culture: cut the sterilized materials into 1 cm long stems with one node and inoculate them in the bud induction medium sterilized by high temperature and h...