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Cloning and application of the strong low temperature-inducible promoter poscold6 in rice

A promoter and rice technology, which is applied in the field of biotechnology and plant genetic engineering, can solve the problems of background expression leakage, insufficient strength, etc., and achieve the effect of improving growth characteristics, improving growth characteristics, and increasing expression

Active Publication Date: 2017-10-13
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although these promoters can respond specifically to the induction of low temperature stress in the environment, there are more or less defects in the leakage of background expression and insufficient strength after induction to support functional genes to fully play the role of cold resistance.

Method used

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  • Cloning and application of the strong low temperature-inducible promoter poscold6 in rice
  • Cloning and application of the strong low temperature-inducible promoter poscold6 in rice
  • Cloning and application of the strong low temperature-inducible promoter poscold6 in rice

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Embodiment Construction

[0029] The present invention will be described below with reference to specific examples. Those skilled in the art can understand that these examples are only used to illustrate the present invention and do not limit the scope of the present invention in any way.

[0030] The experimental methods in the following examples are conventional methods unless otherwise specified. The medicinal raw materials, reagent materials, etc. used in the following examples are all commercially available products unless otherwise specified.

[0031] Acquisition of POscold6 promoter containing restriction site

[0032] Step 1. Design of primers

[0033] According to the whole genome sequence of rice variety Nipponbare (Oryza sativa L cv. Nipponbare) provided by NCBI, the amplification primers were designed according to the sequence of rice POscold6 gene, and the enzyme cutting sites of the primers were designed according to the characteristics of the selected vector and target gene.

[0034...

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Abstract

The invention discloses the cloning and application of a rice low-temperature strongly inducible promoter POscold6. The invention also provides an expression cassette containing the promoter, a plant expression vector, a host bacterium and a transformant and applies it to plant genetic engineering. The inventors of the present application obtained and verified a DNA sequence with special functions—rice low-temperature strongly inducible promoter POscold6 by amplifying, isolating, analyzing, utilizing and experimentally verifying the specific genome of the rice variety Nipponbare. The promoter provided by the present invention can specifically drive the expression of exogenous genes in plants under low temperature / cold stress conditions. Therefore, in genetic engineering, using the promoter to replace the constitutive promoter to drive the cold resistance gene can effectively improve the low temperature Lowering the cold resistance of transgenic plants will avoid the invalid expression of cold resistance genes at room temperature, so as to achieve the best use of cold resistance genes.

Description

technical field [0001] The invention relates to the technical fields of biotechnology and plant genetic engineering. Specifically, the present invention relates to the cloning and application of a rice low temperature strongly inducible promoter POscold6, which can specifically drive the expression of exogenous genes in plants under low temperature / cold stress conditions. Background technique [0002] Rice is an important food crop, and chilling injury is one of the important limiting factors affecting rice production. Chilling damage at the germination stage, seedling stage, and booting flowering stage will lead to stunted growth of rice seedlings, reduced tillering, and ultimately a significant reduction in rice yield. In addition, temperature is also an important environmental factor affecting rice grain quality. Therefore, solving the problem of rice chilling injury has very important practical significance for world food security and promoting the economic development...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/11C12N15/82C12N1/21A01H5/00
Inventor 魏鹏程杨剑波李娟李莉李浩秦瑞英马卉杨亚春许蓉芳
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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