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A rice low temperature inducible expression promoter poscold10 and its application

A low temperature induction and promoter technology, applied in the field of biotechnology and plant genetic engineering, can solve the problems of unfavorable practical application promotion, short plant size, developmental delay, etc., and achieve the effect of improving and improving growth characteristics

Active Publication Date: 2022-03-25
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, in the current genetic engineering operations, constitutive promoters are often used to drive the overexpression of these key node genes. Although the obtained transgenic plants can show strong low temperature tolerance, the unnecessary expression brought about by the constitutive promoters often leads to plant failure. Short stature, developmental delay and waste of material and energy are not conducive to potential practical application promotion

Method used

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  • A rice low temperature inducible expression promoter poscold10 and its application
  • A rice low temperature inducible expression promoter poscold10 and its application
  • A rice low temperature inducible expression promoter poscold10 and its application

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Embodiment Construction

[0026] The present invention will be described below with reference to specific examples. Those skilled in the art can understand that these examples are only for illustrating the present invention, and they do not limit the scope of the present invention in any way.

[0027] The experimental methods in the following examples are conventional methods unless otherwise specified. The biochemical reagents, consumables and raw materials used in the following examples are all commercially available products unless otherwise specified.

[0028] Obtaining of Poscold10 Promoter Containing Restriction Site

[0029] Step 1. Design of primers

[0030] During the research, the applicant accidentally discovered a gene sequence with low temperature-inducible properties, named Poscold10, for the specific sequence, please refer to the sequence listing. The applicant designed amplification primers based on the sequence of rice Poscold10, and designed the restriction enzyme cleavage sites of...

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Abstract

The invention provides a plant low temperature inducible expression promoter Poscold10 and application thereof. The invention also provides expression cassettes, plant expression vectors, host bacteria and transformants containing the promoter. Specifically, the present invention applies the above-mentioned promoters to plant genetic engineering. The promoter provided by the invention can specifically drive the high-intensity expression of exogenous genes in plants under low temperature conditions. By linking the promoter of the present invention to the gene to be expressed and using the carrier to transfer it into the plant plant, it is possible to drive the expression of the foreign gene in various parts of the plant under low temperature conditions.

Description

technical field [0001] The present invention relates to the technical field of biotechnology and plant genetic engineering. Specifically, the present invention relates to a plant low temperature-inducible expression promoter and its application, which can drive the expression of a target gene in a plant in a rice transgenic regulation system. Background technique [0002] Rice is one of the most important food crops in the world. In recent years, due to the climatic El Niño phenomenon, rice has encountered extreme climates such as drought or flood during the growth stage, which has seriously affected the yield of rice. Improving the resistance of crops to extreme climates through plant genetic engineering has important application prospects. The regulation of gene expression by promoters suggests that we can activate the expression of stress resistance genes by changing or adding promoters by genetic engineering, and improve the stress resistance of crops. [0003] Low tem...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/84A01H5/00A01H6/46
CPCC12N15/8237C12N15/8205C12N15/8273
Inventor 许蓉芳李娟秦瑞英刘小双廖圣祥
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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