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A DNA fragment poscold5 capable of responding to long-term cold stress

A DNA sequence and fragment technology, applied in the field of DNA fragments, can solve problems such as unfavorable practical application promotion, waste of material and energy, and short plants, and achieve the effects of improving growth characteristics, increasing expression, and improving growth characteristics

Active Publication Date: 2017-07-25
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the current genetic engineering operations use constitutive promoters to drive the expression of these key genes. Although the obtained transgenic plants can show strong low temperature tolerance, the constitutive promoters often bring some unnecessary Expression, such as causing plant dwarfism, developmental delay and waste of material and energy, is not conducive to potential practical application promotion

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  • A DNA fragment poscold5 capable of responding to long-term cold stress
  • A DNA fragment poscold5 capable of responding to long-term cold stress
  • A DNA fragment poscold5 capable of responding to long-term cold stress

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Embodiment Construction

[0031] The present invention will be described below with reference to specific examples. Those skilled in the art can understand that these examples are only used to illustrate the present invention and do not limit the scope of the present invention in any way.

[0032] The experimental methods in the following examples are conventional methods unless otherwise specified. The medicinal raw materials, reagent materials, etc. used in the following examples are all commercially available products unless otherwise specified.

[0033] Acquisition of POscold5 promoter containing restriction site

[0034] Step 1. Design of primers

[0035] According to the whole genome sequence of the rice variety Nipponbare (Oryza sativa L cv. Nipponbare) provided by NCBI, the amplification primers were designed according to the sequence of the rice POscold5 gene, and the restriction sites of the primers were designed according to the characteristics of the selected vector and target gene.

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Abstract

A DNA fragment POscold5 capable of responding to long-term cold stress, the present invention discloses a DNA fragment comprising: 1) the DNA sequence shown in SEQ ID NO: 1 in the sequence listing; or 2) under stringent conditions and in 1) Said DNA sequence hybridizes and has a DNA molecule with a promoter function; or 3) a DNA fragment that has more than 90% homology with the DNA sequence defined in 1) or 2) and has a promoter function. The DNA fragment can be used as a plant cold-inducible expression promoter. The invention also provides an expression box and a plant expression vector containing the promoter and its application in plant genetic engineering. The promoter has important theoretical and practical significance for the research on the molecular mechanism of cold tolerance of rice and the molecular breeding of cold tolerance of rice. The invention will have broad application and market prospects in the agricultural field.

Description

technical field [0001] The invention relates to the technical fields of biotechnology and plant genetic engineering. Specifically, the present invention relates to a DNA fragment, which can respond to long-term cold stress, and then drive the expression of a target gene in a plant in a rice transgenic regulation system. Background technique [0002] Many crops, especially rice, are extremely sensitive to temperature. The common problem in the main rice producing areas of the world is the problem of low temperature and chilling damage. Chilling damage at the germination stage, seedling stage, and booting flowering stage will lead to stunted growth of rice seedlings, reduced tillering, and ultimately a significant reduction in rice yield. In addition, temperature is also an important environmental factor affecting rice grain quality. Therefore, there is an urgent need to discover cold-tolerant germplasm resources of rice and breed cold-tolerant varieties of rice. [0003] I...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/11C12N15/82A01H5/00
Inventor 魏鹏程杨剑波秦瑞英李莉李浩李娟杨亚春许蓉芳马卉
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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