Plant high temperature inducible expression promoter posheat2 and its application

Abstract

The invention provides a high-temperature-induced expressed paddy rice promoter Posheat2 and an application thereof. The invention further provides an expression kit, a plant expression vector, a host strain and transformant containing the promoter. Specifically, the invention provides an application of the promoter in plant genetic engineering. The promoter provided by the invention can specifically drive the expression of an exogenous gene in a plant under the condition of high-temperature induction, so that the promoter can be used for enhancing and improving the growth characteristics of plants, in particular the high-temperature resistance of the plants, and thus, the high-temperature resisting traits of the plants are effectively improved.

Description

technical field [0001] The invention relates to the technical fields of biotechnology and plant genetic engineering. Specifically, the present invention relates to a plant high-temperature-induced gene expression promoter Posheat2 and its application. In transgenic rice plants, the promoter can specifically drive target gene expression at high temperature. Background technique [0002] With the acceleration of industrialization, the CO produced by the development of human society 2 It is an indisputable fact that global warming is caused by greenhouse gases such as greenhouse gases. In many parts of the world, high temperature has become the main factor affecting crop growth. Relevant data show that extreme weather phenomena such as high temperature in summer will appear more frequently and last longer in many regions of the world. The flowering and fruiting period of the double-cropping early rice and the flowering period of the middle rice in the south of the Yangtze Riv...

AI Technical Summary

Problems solved by technology

Research by Li Chengde et al., Cao Yunying et al. found that when the daily average temperature is higher than 32°C and the daily maximum temperature is higher than 35°C, pollen vigor, anther cracking, pollen germination, and pollen tube elongation will all be affected, resulting in a decrease in the rate of fertilization and seed setting.

Although there have been some studies on the reasons and physiological mechanisms of the reduction of seed setting rate caused by high temperature, most of the current research is limited to one aspect, and there are not many research reports on the improvement or selection of heat-tolerant rice varieties using transgenic engineering technology

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