Application of wolfiporia cocos bacterial culture in termite trap-killing, and termite trap-killing bait

A technology of culture and trapping agent, applied in application, insecticide, biocide and other directions, can solve the problem of lack of ideal trapping and killing agent for underground termites, and achieve the effect of ensuring the effect of termite eradication and reducing the dosage of drugs.

Active Publication Date: 2015-08-26
INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented people's work has shown that certain types of microorganisms from Termitomyces are effective at killing termites by causing them to break down deadly organic material inside they themselves without harming humans or animals nearby. These materials help control damage caused during burrowings for future generations.

Problems solved by technology

This patented technical problem addressed in this patents relates to finding suitable buried termitious materials such as people' s footings or other wooden objects within building structures without causing environmental concerns like soil contamination caused by pesticides.

Method used

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  • Application of wolfiporia cocos bacterial culture in termite trap-killing, and termite trap-killing bait
  • Application of wolfiporia cocos bacterial culture in termite trap-killing, and termite trap-killing bait

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: Extraction of the extract of Poria cocos pine sawdust culture.

[0016] The pine sawdust removes impurities, is baked to a constant weight, and is added with 6% sucrose water of equal mass fraction. After mixing, put it into a high-temperature-resistant polypropylene plastic bag to 2 / 3 of the capacity, put a bamboo tube with an inner diameter of 40mm and a height of 60mm at the mouth of the plastic bag, turn the polypropylene plastic film outward and fasten it with a yarn tape Put on a cotton plug and sterilize it in a sterilization pot for 90 minutes. After the sterilization pot was cooled to room temperature, the pine sawdust was taken out, and the Wolfiporia cocos (Schw.Ryv.&Gibn) bacterial liquid was inserted under aseptic operation conditions, and incubated at 22~28℃ for 45 days. The culture was terminated when the whole polypropylene plastic film bag was covered, and the Poria cocos pine sawdust culture was obtained. The Poria cocos pine sawdust culture w...

Embodiment 2

[0019] Example 2: Determination of the activity of the extract concentrate of the Poria cocos pine sawdust culture against C. taiwanensis.

[0020] Use ground glass as the bottom plate, use n-hexane solvent, and the n-hexane diluent of the Poria cocos pine sawdust culture extract concentrate (obtained according to the method in Example 1) diluted 6 times with n-hexane, respectively circled with n-hexane solvent In the inner circle, the diameter is 110mm, the n-hexane diluent of the extract of the Poria cocos pine sawdust culture extract concentrate is on the outer circle, the diameter is 150mm, in the center of the circle, 5 C. termite workers are placed and quickly covered with a glass petri dish with a diameter of 70mm After the termite workers settle down, open the petri dish, let the termites move randomly, observe and record the direction of the termite workers, and take half of the circle drawn by each worker ant after the n-hexane diluent of the Tuckahoe fungus pine sawdust...

Embodiment 3

[0025] Example 3: Determination of the weight loss rate of Poria cocos culture medium and the activity response value of C. taiwanensis

[0026] According to the method of Example 1, the Poria cocos were inoculated into pine wood chips of known constant weight, and inoculated a total of 20 bags, divided into 10 parts and cultivated 25, 30, 35, 40, 45, 50, 55, 60 at 25℃. , 65, and 70 days to obtain the Poria cocos pine sawdust culture. After the cultivation, part of the poria cocos pine sawdust culture was dried to a constant weight and the weight loss rate of the pine sawdust was calculated. Then according to the method of Example 1 to obtain the Poria cocos pine sawdust culture extract concentrate, and then according to the method in Example 2 to determine the activity of the Taiwan C. termites against the Poria cocos pine sawdust culture extract concentrate with n-hexane dilution In response, the measurement results show that the culture with a weight loss rate of 15-30% has t...

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Abstract

The invention discloses an application of a wolfiporia cocos bacterial culture in termite trap-killing, and a termite trap-killing bait. The wolfiporia cocos bacterial culture and extract thereof have a significant trapping effect upon termites. The trapping effect exceeds those of previously reported other bacteria, rot fungi and culture extracts thereof. Therefore, the wolfiporia cocos bacterial culture and the extract thereof can be used for luring termites, such that termite intake can be accelerated, and food consumption can be increased. When the wolfiporia cocos bacterial culture is added into a termite trap-killing bait, the termite killing effect of the bait can be ensured, and dose can be reduced. The application also has an environment protection significance.

Description

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Claims

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Application Information

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Owner INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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