Screening Method of Virus-free Potato Plantlets Against Early Blight
A screening method and potato technology, which is applied in the field of screening anti-early blight materials, can solve the problems of unsuitable evaluation of virus-free potato plantlets against early blight, large early blight deviation, and long detection time, achieving small errors and short screening time. Short, low screening cost effects
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Embodiment 1
[0015] Embodiment 1, the screening method of this detoxified potato test-tube plantlet anti-early blight, is carried out according to the following steps: the first step, the isolation of pathogenic bacteria, collects the potato early blight diseased leaf in the field of different regions, gathers the potato early blight diseased leaf After rinsing with tap water for 1 to 3 times, soak in ethanol aqueous solution for 20s to 30s after soaking, then soak in mercuric chloride aqueous solution for 10s to 20s, rinse with sterile water for 3 to 5 times, and then use sterilized Take out the leaves at the junction of disease and health with a bacterial scalpel, place the back of the leaves at the junction of disease and health on the PDA plate medium, put 3 to 4 leaves in each dish, and place them in an incubator at 25°C for 3 days to 3 days in the dark. 5d, after culturing, isolate the pathogenic bacteria on the leaves to obtain the pathogenic bacteria;
[0016] The second step is th...
Embodiment 2
[0021] Example 2, as an optimization of the above example, the PDA medium is obtained as follows: Weigh 200g of fresh potatoes, wash and peel the slices, add 1000mL of deionized water and boil for 15min, filter with gauze and add deionized water to make up to 1000mL , then add 12g glucose and 10g to 12g agar powder, boil to obtain PDA medium after the agar powder is completely melted. Boil until the agar powder is completely melted, then subpackage; the subpackaged PDA medium should be sterilized within 1 hour, using high-pressure steam for moist heat sterilization, under 0.1MPa steam pressure, the temperature reaches 121°C, and maintains for 20 minutes.
[0022] Packing method: A. Divided into Erlenmeyer flasks (conical flasks); use 250 mL Erlenmeyer flasks for dispensing, each bottle is divided into about 200 mL of culture medium, and the mouth of the bottle is covered with a ventilated and sterilized sealing film, which is tied tightly with cotton thread.
[0023] B. Test...
Embodiment 3
[0026] Embodiment 3, as the optimization of above-mentioned embodiment, the blade size of sick and healthy junction is 0.5cm 2 to 1cm 2 .
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