Preparation method of mycelium extract of strain culture of marine fungus umbelopsis sp.

A marine fungus and mycelium technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of rotten use of antibiotics, large toxic and side effects, and strong drug resistance

Inactive Publication Date: 2015-11-25
QINZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current antifungal drugs still have the shortcomings of lack of types, low selectivity, large toxic and side effects, and strong drug resistance. Therefore, it is necessary to research and develop new, efficient, low-toxicity, and broad-spectrum antifungal drugs.
[0005] In the research of antibacterial drugs, from the emergence of the first antibacterial drug penicillin, to the later tetracycline, chloramphenicol, kanamycin, rifampicin and other antibiotics, all played an important role in the fight against pathogenic bacteria in humans. But with the rotten use of antibiotics

Method used

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  • Preparation method of mycelium extract of strain culture of marine fungus umbelopsis sp.
  • Preparation method of mycelium extract of strain culture of marine fungus umbelopsis sp.
  • Preparation method of mycelium extract of strain culture of marine fungus umbelopsis sp.

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Isolation and identification of the marine fungus Umbelopsis sp. QZ042

[0037] 1. Isolation of Fungi

[0038] Take a sample of marine rot wood in a sterile conical flask, weigh 5g, mash it with a sterile glass rod, add 50ml of sterile saline, add glass beads and shake on a shaker for 20min, after standing for stratification, take the upper layer to make 10 times Gradient dilution, take the original solution, 10 times and 100 times dilutions Tubu PDA solid medium, culture at a constant temperature of 26 ~ 30 ℃, after the colony grows, pick the colony and streak to purify it.

[0039] 2. Fungal culture and morphological observation

[0040] Observation of culture characteristics: the strains were inoculated on PDA medium, and cultured at 26°C to obtain colonies. Record the color of the primary hyphae, the color change of the colony, the surface characteristics, and the presence or absence of pigmentation.

[0041] Film production and microscopic examination...

Embodiment 2

[0056] Embodiment 2: Extraction of active substance of marine fungus Umbelopsissp. QZ042

[0057] 1. Seed culture

[0058] Medium: 180-220g of potatoes, 18-22g of glucose, 20g of agar, 1000mL of 50% artificial seawater. Fungal hyphae were picked and inoculated on the culture medium for slant culture, and cultured at 28°C for 5-7 days. Wherein, the formula of artificial seawater (salinity 3.34%) is as follows: every liter of water contains: sodium chloride NaCl26.726g, magnesium chloride MgCl 2 2.26g, magnesium sulfate MgSO 4 3.248g, calcium chloride CaCl 2 1.153g, sodium bicarbonate NaHCO 3 0.198g, potassium chloride KCI0.721g, sodium bromide NaBr0.058g, boric acid H 3 BO 3 0.058g, sodium silicate Na 2 SiO 3 0.0024g, sodium silicate Na 2 Si 4 o 9 0.0015g, phosphoric acid H 3 PO 4 0.002g, aluminum hexachloride Al 2 Cl 6 0.013g, ammonia NH 3 0.002g, lithium nitrate LiNO 3 0.0013g.

[0059] 2. Fermentation culture

[0060] Fermentation medium: 180-220g of potat...

Embodiment 3

[0064] Example 3: Antibacterial activity of mycelium extracts from cultures of the marine fungus Umbelopsis sp. QZ042

[0065] 1. Preparation of indicator bacteria

[0066] Inoculate E.Coli, B.cereus, Arthrobacternicotianae, Klebsiellapeneumoniae, and Vibrioparahaemolyticus in beef extract-peptone liquid medium respectively, and culture them overnight on a shaker at 37°C at a speed of 150r / min. The indicator fungi Eurotiumrubrum, Alternariasp., Lasiodiplodiapseudotheobromae, Diaporthephaseolorum, and Pestalotiopsismicrospora were inoculated on PDA plates and cultured to 1 / 2 of the petri dish. These bacteria and fungi are preserved by our laboratory. Inoculate 2 plates for each indicator strain.

[0067] 2. Determination of Antibacterial Activity

[0068] Weigh the mycelium extract prepared in Example 2, and dilute the solution to 400 μg / ml, 200 μg / ml, 100 μg / ml, 50 μg / ml, 25 μg / ml, 12.5 μg / ml, 6.25 μg by adding sterile water / ml, and its antibacterial activity was determin...

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Abstract

The invention discloses an Umbelopsis sp. strain and a mycelium extract thereof, as well as an application. The Umbelopsis sp. QZ042 was saved in China General Microbiological Culture Collection Center (CGMCC) on August 19, 2013 with the collection number of CGMCC No. 8101. The applicant finds that, the mycelium extract of an Umbelopsis sp. QZ042 culture disclosed by the invention has relatively good anti-bacterial activity against colon bacillus, bacillus cereus, arthrobacter nicotianae, klebsiella, bibrio parahemolyticus medicaments and other bacteria and has obvious antibacterial effect against eurotiales, alternaria, lasiodiplodia, stem canker fungi and other types of fungi through experiments and screening medicaments are provided for researching and developing new anti-bacterial and anti-fungal medicaments.

Description

[0001] This application is a divisional application of "a marine fungus Mycelia strain and its mycelium extract and its application". The filing date of the original application is: May 15, 2014, the application number is: 201410203253.9, and the invention name is : A marine fungus Cladomycetes strain and its mycelium extract and application. technical field [0002] The invention relates to marine microbial strains, in particular to a marine fungus Cladomycetes strain and its mycelium extract and application. Background technique [0003] Pathogenic bacteria and fungi can cause various diseases of animals and plants, which not only affect crop yields and economic animal growth and production, causing great economic losses; but also affect human health and threaten human life safety. In order to inhibit and eliminate pathogenic bacteria and fungi, people have been working on finding new and effective antibacterial drugs from different environments to alleviate the emergence ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A61K36/06A61P31/04A61P31/10C12R1/645
CPCY02A50/30
Inventor 龚斌义翠花张艳秋方怀义庞庭才张虹董庆亮黄鹄
Owner QINZHOU UNIV
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