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A kind of aatsw2 gene promoter and its application and preparation method

A promoter and gene technology, applied in the field of genetic engineering, can solve problems such as harm and normal plant growth burden

Active Publication Date: 2017-12-29
SHANGHAI JIAOTONG UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

This promoter can drive the expression of foreign genes in all tissues and organs, so it is likely to cause a certain burden and harm to the normal growth of plants

Method used

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  • A kind of aatsw2 gene promoter and its application and preparation method
  • A kind of aatsw2 gene promoter and its application and preparation method
  • A kind of aatsw2 gene promoter and its application and preparation method

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Embodiment Construction

[0031] The specific embodiments provided by the present invention will be described in detail below in conjunction with the accompanying drawings.

[0032] The experimental method that does not indicate specific conditions in the following examples is usually according to conventional conditions, such as molecular cloning such as Sambrook: the laboratory manual sees the conditions described in the 1989 edition of New York:Cold Spring Harbor Laboratory Press, or according to the manufacturer suggested conditions.

[0033] The Agrobacterium tumefaciens EHA105 involved in this embodiment has been described in "Huang Yali, Jiang Xiliang, Tian Yunlong, Guo Ping, Zhu Changxiong; Research on the genetic transformation of Trichoderma harzianum mediated by Agrobacterium tumefaciens, Chinese Journal of Biotechnology, 2008, 28( 3): 38-43" published in the literature. The plasmid pCAMBIA1391Z of Agrobacterium tumefaciens EHA105 can be obtained through public commercial channels, for exam...

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Abstract

The present invention discloses an AaTSW2 gene promoter and its preparation method. The nucleotide sequence of the promoter is shown in Sequence 1 in the sequence table; meanwhile, the present invention also relates to the expression and production of the AaTSW2 gene promoter using plant glandular hair tissue Application of metabolites in genetic engineering breeding. The promoter provided by the present invention can guide the specific expression of genes in plant glandular trichomes, because the glandular trichome-specific expression promoter can genetically manipulate the glandular trichome system of plants, and will not cause harm to the growth and development of plants. Genetic engineering breeding for the expression and production of metabolites in glandular trichomes is of great significance.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a gene promoter and its application and preparation method. Background technique [0002] Artemisia annua L. is an annual herb belonging to the family Asteraceae. Artemisinin is a sesquiterpene lactone compound containing a peroxide bridge structure isolated from its aerial part. It is currently recognized as the most effective drug for treating malaria in the world, especially for cerebral malaria and chloroquine-resistant malaria. efficient. Currently, the most effective treatment for malaria recommended by the World Health Organization is artemisinin combination therapy (ACTs). At present, the main source of artemisinin is extracted from the aerial parts of Artemisia annua plants. However, the content of artemisinin in Artemisia annua is very low (0.01%-1%), which limits the large-scale commercial production of this drug. [0003] AaTSW2 is a WRKY transcription ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/82C12N1/21A01H5/00
Inventor 唐克轩陈明慧颜廷祥付雪晴石璞马亚男郝小龙孙小芬
Owner SHANGHAI JIAOTONG UNIV
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