A promoter for regulating gene expression in non-secretory glandular hairs and its application

A non-secreting glandular hair and gene regulation technology, applied in the field of promoters, can solve the problems of normal growth burden of plants, excessive consumption of cell material and energy, and regulation of target genes in time and space.

Active Publication Date: 2017-12-01
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the current genetic engineering research of Artemisia annua, constitutive promoters are often used, such as the cauliflower mosaic virus 35S promoter (CaMV35S), which can drive the expression of foreign genes in all tissues and organs, and will cause excessive consumption. The substance and energy in the cell, and the expression of the target gene cannot be regulated in time and space, which is likely to cause a certain burden and harm to the normal growth of plants

Method used

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  • A promoter for regulating gene expression in non-secretory glandular hairs and its application
  • A promoter for regulating gene expression in non-secretory glandular hairs and its application
  • A promoter for regulating gene expression in non-secretory glandular hairs and its application

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Embodiment

[0038] This embodiment relates to obtaining the AaPDS1 gene promoter, which specifically includes the following steps:

[0039] Step 1: Cultivate sterile seedlings of Artemisia annua

[0040] The seeds of Artemisia annua were soaked in 75% ethanol by volume for 1 minute, and then soaked in 20% (w / v) NaClO for 20 minutes, rinsed with sterile water for 3 to 4 times, and dried with sterile absorbent paper for inoculation Cultivate it on a hormone-free MS solid medium at 25°C, 16h / 8h (light / dark) light, and you can get sterile A. annua seedlings after 14 days;

[0041] Step 2: Cloning of the promoter sequence in genomic DNA

[0042] 1. Extraction of genomic DNA

[0043] Put a piece of Artemisia annua leaf (1cm) in a 1.5mL centrifuge tube 2 Left and right size, use ice box to hold), add 2 steel balls. Add 300μL TPS buffer (operated in a fume hood, TPS contains mercaptoethanol), shake for 90 seconds at 55-60 Hz. Then add 300μL TPS buffer (fume hood). Water bath at 65℃ for 1h (shaking ever...

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Abstract

The invention discloses a gene promoter in the field of plant biotechnology. The nucleotide sequence of the promoter is shown in SEQ ID NO: 1, which can regulate the specific expression of target genes in non-secretory glandular hairs of plants. At the same time, the present invention also relates to the use of the aforementioned gene promoter in genetic engineering breeding using plant glandular hair tissue to express and produce metabolites. Since the glandular hair system of the plant is genetically manipulated by using the specific expression promoter of the plant glandular hair, it will not cause harm to the growth and development of the plant. Therefore, the present invention is of great significance to genetic engineering breeding using plant glandular hair tissue to express and produce metabolites.

Description

Technical field [0001] The present invention relates to a promoter, in particular to a terpene synthase gene promoter (proFDS1) specifically expressed in plant non-secreted glandular hairs obtained by molecular biology and its application in transgenic plants . Background technique [0002] Artemisia annua (Artemisia annua L.) is an annual herb of the genus Artemisia in the Compositae family. The plant has a strong volatile aroma and contains many secondary metabolites: artemisinin, volatile oil, α-pinene, camphor, and Artemisia annua Ketones, etc., in addition to flavonoids. Artemisinin (Artemisinin) is a sesquiterpene lactone compound containing a peroxy bridge structure isolated from its aerial part. It is used as the World Health Organization (WHO) recommended antimalarial drug combination therapy (Artemisinin-based combination therapies, ACTs) the main active ingredients. At present, the main source of artemisinin is extracted from the aerial part of Artemisia annua. Howe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/82C12Q1/68A01H5/00
Inventor 唐克轩石璞付雪晴沈乾唐岳立孙小芬吕宗友颜廷祥
Owner SHANGHAI JIAO TONG UNIV
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