38 results about "ARTEMISIA ANNUA LEAF" patented technology

The invention discloses a method for extracting artemisinin. The method comprises the following steps: crushing leaf of Artemisia carvifolia; carrying out enzymatic hydrolysis in an aqueous solution, and carrying out pumping filtration after the enzymatic hydrolysis; carrying out low temperature extraction of the obtained filter cake by using an organic solvent, and concentrating the obtained extract liquid to obtain crude artemisinin; and recrystallizing the crude artemisinin to obtain purified artemisinin. Compared with traditional extraction methods and supercritical extraction methods, the method adopting an enzymatic hydrolysis-reflux two-step extraction technology in the invention has the advantages of simple process, less impurities, low apparatus requirements, and obviously increased extraction rate, and is an environmentally-friendly and efficient artemisinin extraction method. The method is also suitable for extracting sesquiterpene lactones in other medicinal plants.

The invention discloses a method for efficiently separating and instantaneously converting a protoplast of artemisia annua L., comprising the following steps: 1) selecting leaves of young and tender artemisia annua and removing lower epidermis, to obtain the leaves of the artemisia annua L. with the lower epidermis removed; 2) placing the leaves of the artemisia annua L. with the lower epidermis removed in an enzymatic hydrolysate for enzymatic hydrolysis to obtain a mixed solution of enzymatic hydrolysis; 3) filtering and centrifuging the mixed solution of enzymatic hydrolysis to obtain a protoplast precipitate, and resuspending to obtain a protoplast; and 4) instantaneously converting the protoplast. The method for separating and converting the protoplast is constructed in the artemisia annua L. for the first time, to obtain the protoplast with yield of 2.749 is multiplied by 10<5> / g FW, viability of 96%, and conversion efficiency of green fluorescent protein of 80%, and the obtained protoplast has large yield and high viability. The protoplast of the artemisia annua L. is utilized as a receptor for conversion, and green fluorescent protein GFP, luciferase LUC and sea cucumberluciferase REN can be successfully expressed.

The invention discloses an observation and density measurement method for glandular hairs on Artemisia annua leaf surfaces, comprising the following steps: taking a eugonic Artemisia annua leaf, and feeding the leaf in a centrifuge tube or a microtiter plate; successively adding petroleum ether or normal hexane, absolute ethyl alcohol or carbinol and glacial acetic acid, evenly mixing, and incubating for 5-20min at room temperature; and taking out the leaf, placing the left on a glass slide, adding a drop of double distilled water, covering a cover slip slightly, compacting by a tablet clip, and observing on an optical microscope or calculating the number of the glandular hairs so as to calculate the density of the glandular hairs. The method is simple and convenient, has low requirement on equipment, greatly simplifies the preprocessing procedure of experimental materials, can complete the whole operation within half a hour, uses a little of reagent, is non-toxic, safe and environment-friendly, lowers the measurement cost and time substantially, and can be used for high pass analysis.

The invention discloses and provides a leaf surface resistance and control agent for blocking heavy metal accumulation and a preparation method thereof. The leaf surface resistance and control agent is prepared from the following components in parts by weight: 1-3 parts of borax, 5-9 parts of nano silicon dioxide, 10-17 parts of carbon nano tubes, 5-8 parts of a surfactant, 18-31 parts of tea seedcake, 7-9 parts of dunaliella, 13-22 parts of flammulina velutipes fungus chaff, 3-5 parts of betel nut tree sawdust, 9-13 parts of a microbial agent, 3-4.5 parts of orange peel powder, 8-13 parts ofdistillers' grains, 7-10 parts of artemisia apiacea leaves, 25-43 parts of tea saponin, 1-3 parts of citric acid, 12-28 parts of amino acids, 6-11 parts of humic acid and 1000-1500 parts of deionizedwater. The leaf surface resistance and control agent prepared from the raw materials can prevent plants from absorbing heavy metals in air and soil and prevent the heavy metals from damaging the plants. Meanwhile, according to the preparation method of the leaf surface resistance and control agent for blocking heavy metal accumulation, effective components in the raw materials can be fully utilized, and the method is easy to operate and beneficial to industrial promotion.

The invention relates to a method for preparing an antimalarial active compound artemisinin through direct column chromatography and belongs to the field of drug preparation techniques. The method comprises the steps of: weighing 1 part (by weight) of artemisia annua leaf powder which is sieved by a sieve with the particle size of 100 meshes, weighing 0.7-1.4 parts (by weight) of silica gel or alumina which is sieved by a sieve with the particle size of 80-100 meshes, mixing the artemisia annua leaf powder and the silica gel or alumina, and grinding uniformly; coating the substance, obtained in the last step, in a glass chromatography column containing 0.8-5.0 parts (by weight) of silica gel or alumina which is sieved by the sieve with the particle size of 80-100 meshes, eluting in a manner that petroleum ether-ethyl acetate, or petroleum ether-acetone, or chloroform-acetone, or chloroform-ethyl acetate or the like, which are in the volume ratio of 85: (10-20), serve as a moving phase, and collecting fractions; and carrying out depressurized concentration on a fraction containing artemisinin, and crystallizing by using petroleum ether, thereby obtaining white needle-shaped artemisinin crystals. Compared with the prior art, the method has the advantages that the extraction and purification process for artemisinin is optimized, the solvent extraction operating steps and the types of used solvents are reduced, and the consumption of the solvents is reduced; and the obtained product has the advantages of stable quality, high yield, low cost and short consumed time.

The invention discloses an environmentally friendly microbial pesticide for killing acarid, and the environmentally friendly microbial pesticide comprises the following components by weight: 1-3 parts of monoucleosis powder, 0.5-1.0 part of bacillus subtilis powder, 8-12 parts of herba ephedrae, 1-2.5 parts of raw ginger, 2.5-3.5 parts of erodium stephanianum willd, 20-35 parts of xanthium, 30-40 parts of pomegranate rind, 1-3 parts of mugwort leaf, 11-21 parts of cordate houttuynia, 9-16 parts of andrographis paniculata, 3-8 parts of hemp, 7-9 parts of serrate chloranthus, 9-13 parts of artemisia annua leaf, 2-5 parts of tobacco, 3-8 parts of radix phytolaccae, 7-12 parts of dandelion, 11-13 parts of leech, 8-13 parts of croton, 3-6 parts of polygonatum odoratum, 2-4 parts of nard, 1-3 parts of licorice and 3-9 parts of euphorbia kansui. The environmentally friendly microbial pesticide has characteristics of both a biological pesticide and a plant pesticide, if a synergist is added, the relationship of the pesticide efficacy and the components is geometrically progressed, the experimental data shows that the pesticide efficacy is 10-20 times of the efficacy of a single component, and the environmentally friendly microbial pesticide is low in toxicity to human and birds.

The invention discloses a microbial green environmentally-friendly pesticide comprising the following components by weight: 2-6 parts of pityrosporion ovale powder, 10-25 parts of escherichia coli powder, 5-6 parts of bacillus subtilis powder, 8-12 parts of ephedra, 1-2.5 parts of raw ginger, 2.5-3.5 parts of erodium stephanianum willd, 20-35 parts of cocklebur fruit, 30-40 parts of pomegranate peel, 12-32 parts of folium artemisiae argyi, 11-21 parts of cordate houttuynia, 9-16 parts of andrographis paniculata, 3-8 parts of hemp, 7-9 parts of serrate chloranthus, 9-13 parts of artemisia annua leaf, 2-5 parts of tobacco, 3-8 parts of phytolacca americana, 10-2 parts of radix stemonae ,15-20 parts of chinaberry fruit, 4-10 parts of camphor, 3-4 parts of an emulsifier, 5-6 parts of turpentine and 80 parts of water. The microbial green environmentally-friendly pesticide has the beneficial effects that: the microbial green environmentally-friendly pesticide has the contact killing and stomach poisoning effects, is free of toxicity, pollution and residue, non-pollution, safe to human and livestock and birds, does not produce injury to plants, and is broad-spectrum in insecticidal effects and strong in penetration.

The invention discloses a microbial agricultural traditional Chinese medicine pesticide comprising the following components by weight: 5-13 parts of lactobacillus powder, 1-3 parts of nitrobacteria powder, 5-6 parts of nodule bacteria powder, 8-10 parts of monoucleosis powder, 9-13 parts of candida powder, 4-13 parts of rhodococcus powder, 7-13 parts of chrysanthemum, 9-13 parts of hemsleya amabilis, 1-7 parts of antifebrile dichroa, 3-8 parts of snake slough, 3-7 parts of grifola, 5-9 parts of marijuana, 5-8 parts of serrate chloranthus, 5-12 parts of artemisia annua leaf, 1-6 parts of caffeine, 1-10 parts of a synergistic agent, 20-30 parts of tobacco, 10-15 parts of pokeweed, 10-20 parts of aconitum and 100 parts of water. The microbial agricultural traditional Chinese medicine pesticide is a new pesticide which is high-efficiency, purposeful, environmentally-friendly, free of secondary toxicity, residue and pollution, can keep ecological balance, is an important guarantee for realization of ecological agriculture, green ecological agriculture and green enterprise, and has broad prospects.

The invention discloses a microbial pesticide comprising the following components by weight: 1-5 parts of matrine, 1-3 parts of aphid killing agent powder, 2-4 parts of hirsutella thompsonii fisher powder, 3-6 parts of verticicladiella powder, 5-6 parts of serrate chloranthus, 15-20 parts of artemisia annua leaf, 20-30 parts of tobacco, 10-15 parts of pokeweed, 10-20 parts of aconitum, 12-18 parts of herba houttuyniae, 5-18 parts of herba andrographitis, 4-10 parts of camphor, 10-15 parts of pomegranate peel, 6-9 parts of folium artemisiae argyi, 10-20 parts of cyrtomium fortunei, 12-16 parts of radix sophorae flavescentis, 3-6 parts of herba violae, 5-9 parts of bean root and 100-150 parts of water. The microbial pesticide is harmless to human and livestock, produces no phytotoxicity to crops, has no pesticide residue and no environmental pollution, has the advantages of high efficiency, low toxicity, long period of preserving effect, and also has a certain yield increasing effect.

The invention provides an artemisia apiacea flour product and a production method of the artemisia apiacea product. According to the method, artemisia apiacea flour with the mass percentage being 0.5 percent to 10 percent is added, and the artemisia apiacea flour is obtained through sequentially carrying out clean washing, water removing, dewatering baking, sterilization and crushing on artemisia apiacea leaves. The production method comprises the following steps that flour, artemisia apiacea flour and auxiliary materials are uniformly mixed, warm water accounting to 30 to 55 percent of the weight of mixed raw materials with the temperature being about 30 DEG C is added, and the dough making is carried out for 15 to 20 minutes. The obtained dough is subjected to curing, tabletting, noodle cutting and drying to be made into noodles. Yeast is added into the dough, and bread is obtained through fermentation, forming and baking. The artemisia apiacea flour is added, so the bread and the noodles have the health care effect of the artemisia apiacea. Through the reasonable addition and production process, active ingredients of the artemisia apiacea are maintained to the maximum degree, meanwhile, the natural green color and the fragrance of the artemisia apiacea are remained, the mouth feeling is fresh and delicious, and the artemisia apiacea flour product belongs to rare green health care food.

The invention discloses an agricultural green microbial pesticide which is composed of the following components in parts by weight: 1-3 parts of bdellovibrio powder, 1-3 parts of Escherichia coli powder, 8-12 parts of ephedra, 1-2.5 parts of ginger, 2.5-3.5 parts of Erodium stephanianum Willd, 20-35 parts of cocklebur fruit, 30-40 parts of pomegranate rind, 1-3 parts of folium artemisiae argyi, 11-21 parts of herba houttuyniae, 9-16 parts of creat, 3-8 parts of marijuana, 7-9 parts of chloranthus serratus, 9-13 parts of Artemisia annua leaf, 2-5 parts of tobacco, 3-8 parts of Phytolacca acinosa, 80-120 parts of neem nut and 100 parts of water. The environment-friendly biological pesticide disclosed by the invention is a novel pesticide which has high efficiency, pertinence, environment friendliness, no secondary toxicity, no residue and no pollution, keeps ecological balance and is nuisance-free, is an important guarantee for realizing ecological agriculture, green ecological agriculture and green enterprises, and has wide prospects.

The invention discloses a microbial pesticide for beauveria bassiana and a preparation method of the microbial pesticide. The microbial pesticide for the beauveria bassiana is prepared from the following raw materials: the beauveria bassiana, nicotine, diatomite, metarhizium anisopliae, baicalein, bdellovibrio bacteriovorus, talcum powder, bacillus, vermiculite, sodium carboxymethylcellulose, a plant growth regulator, aluminum oxide, baicalein, garlic oil, matrine, starch, onion, oxymycin, streptomycin, radix chloranthi serrati, artemisia annua leaf, radix phytolaccae, radix aconiti agrestis, rhizoma coptidis, herba houttuyniae, camphor, folium artemisiae argyi, herba violae and a trace element compound. The pesticide disclosed by the invention has the advantages of simple raw material proportion, greenness, environment friendliness and low cost; in addition, the immune ability of plants can be improved, and the pollution to ecological environment is avoided; besides, effective components are complex and diversified, so that the pests cannot generate drug resistance to one or several ingredients, thereby a better prevention and control effect is realized; a production technology is simple and convenient.

The invention discloses a nuisanceless green microbial pesticide for fruits and vegetables, and the pesticide is characterized by comprising the following components in parts by weight: 0.01-0.03 parts of bacteriophage powder, 0.01-0.03 parts of green muscardine fungus powder, 20-25 parts of coptis root, 10-15 parts of pepper, myrrh, myrobalan, 20-30 parts of marijuana, 10-20 parts of serrate chloranthus root, 15-20 parts of artemisia annua leaf, 20-30 parts of tobacco, 10-15 parts of phytolacca, 10-20 parts of radix stemonae, 15-20 parts of chinaberry, 4-10 parts of camphor, 12-18 parts of houttuynia cordata, 5-18 parts of andrographis paniculata, and 100-150 parts of water. The novel nuisanceless residual-free green pesticide for the fruits and the vegetables is a novel efficient, targeted, environment-friendly, secondary-toxicity-free, residual-free, pollution-free and nuisanceless pesticide which maintains ecological balance, is an important guarantee for realizing ecological agriculture, green ecological agriculture, and green enterprises, and has a broad prospect.

The invention discloses a method for extracting artemisinin from sweet wormwood. The method comprises the following steps: drying sweet wormwood leaves in the air, grinding the dried sweet wormwood leaves, adding 3 to 6 times of ethanol into the powder, and stirring in an ultrasonic stirrer for 20 to 30 minutes, so as to obtain a mixture liquid; putting the mixture liquid into a reflux unit for reflux extraction, so as to obtain an extracting solution; conducting vacuum concentration on the extracting solution, adjusting the pH value to be 3 to 4 with citric acid, and drying to obtain an artemisinin crude product; treating the crude product with silica-gel column chromatography, dissolving the dried solid with methanol, and carrying out recrystallization, filtering and drying to obtain high-purity artemisinin. The artemisinin extraction rate can reach 93 percent, and the purity of obtained artemisinin can reach 98.5 percent or higher. The method is simple to operate, low in energy consumption, short in extracting time, high in extracting rate, high in product purity, low in cost, and easy to realize industrial production.

The invention discloses a microbial agricultural pesticide which is composed of the following components in parts by weight: 5-13 parts of lactobacillus powder, 1-3 parts of nitrobacterium powder, 5-6 parts of rhizobium powder, 0.1-0.5 part of minor element, 2.5-3.5 parts of tomatidine, 3-6 parts of radix rhapontici, 15-20 parts of radix angelicae, 10-20 parts of schisandra fruit, 1-10 parts of synergist, 5-20 parts of dandelion, 4-10 parts of purslane, 11-15 parts of folium artemisiae argyi, 12-18 parts of herba houttuyniae, 5-18 parts of creat, 15-20 parts of Artemisia annua leaf, 20-30 parts of tobacco, 10-15 parts of Phytolacca acinosa, 10-20 parts of aconite and 100 parts of water. The pesticide disclosed by the invention is a novel pesticide which has high efficiency, pertinence, environment friendliness, no secondary toxicity, no residue and no pollution, keeps ecological balance and is nuisance-free, is an important guarantee for realizing ecological agriculture, green ecological agriculture and green enterprises, and has wide prospects.

The invention discloses green forest pesticide which is characterized by comprising the following ingredients in parts by weight: 0.01-0.03 parts of meilingmycin powder, 1-10 parts of synergist, 5-20 parts of dandelion, 9-10 parts of radix bupleuri, 1-3 parts of codonopsis pilosula, 10-20 parts of chloranthus serratus, 15-20 parts of artemisia annua leaf, 20-30 parts of tobacco, 10-15 parts of radix phytolaccae, 1-5 parts of emolsifier, 1-10 parts of turpentine, 150-200 parts of matrine, 100-150 parts of nicotine and 1-10 parts of synergist. The novel green forest and flower pesticide is efficient, specific, environment-friendly, nuisanceless, and free from secondary toxicity, residues, and pollution, and keeps ecological balance.

The invention discloses a microbial agricultural traditional Chinese medicine emulsion comprising the following components by weight: 1-3 parts of bdellovibrio powder, 1-3 parts of escherichia coli powder, 2-4 parts of bacillus subtilis powder, 1-2parts of bacteriophage powder, 4-9 parts of hydrargyri oxydum rubrum, 3-5 parts of safflower, 12-16 parts of dwarf lilyturf, 1-3 parts of malt, 7-10 parts of ferulic, 3-7 parts of artemisia annua leaf, 4-8 parts of pepper, 15-20 parts of trifoliate-orange, 8-12 parts of radix stemonae, 1-10 parts of a synergistic agent, 50-80 parts of herba agrimoniae, 40-60 parts of radix euphorbiae lantu, 80-120 parts of China-berry and 100 parts of water. The environmentally-friendly biopesticide is a new pesticide which is high-efficiency, purposeful, environmentally-friendly, free of secondary toxicity, residue and pollution, can keep ecological balance, is an important guarantee for realization of ecological agriculture, green ecological agriculture and green enterprise, and has broad prospects.

The invention discloses an agricultural microorganism / Chinese herbal medicine composite pesticide preparation which is composed of the following components in parts by weight: 1-3 parts of bdellovibrio powder, 3-8 parts of Escherichia coli powder, 4-9 parts of Bacillus subtilis powder, 7-10 parts of asafetida, 3-7 parts of Artemisia annua leaf, 4-8 parts of pepper, 15-20 parts of trifoliate orange, 1-10 parts of turpentine, 10-15 parts of penetrant, 1-10 parts of synergist, 5-20 parts of dandelion, 1-3 parts of gardenia, 2-4 parts of wolfberry, 0.5-1.5 parts of persimmon calyx, 1-2 parts of Mangnolia officinalis, 5-8 parts of herba agrimoniae, 4-6 parts of Radix Euphorbiae Ebractealatae, 8-12 parts of neem nut and 80-90 parts of water. The environment-friendly biological pesticide disclosed by the invention is a novel pesticide which has high efficiency, pertinence, environment friendliness, no secondary toxicity, no residue and no pollution, keeps ecological balance and is nuisance-free, is an important guarantee for realizing ecological agriculture, green ecological agriculture and green enterprises, and has wide prospects.

The invention discloses a forestry green pesticide comprising the following components by weight: 1-3 parts of green muscardine fungus powder, 2-4 parts of hirsutella thompsonii fisher powder, 3-6 parts of verticicladiella powder, 5-6 parts of serrate chloranthus, 15-20 parts of artemisia annua leaf, 20-30 parts of tobacco, 10-15 parts of pokeweed, 10-20 parts of aconitum, 12-18 parts of herba houttuyniae, 5-18 parts of herba andrographitis, 4-10 parts of camphor, 10-15 parts of pomegranate peel, 6-9 parts of folium artemisiae argyi, 12-18 parts of herba houttuyniae, 5-18 parts of herba andrographitis and 100 parts of water. The forestry green pesticide for trees and flowers is a new pesticide which is high-efficiency, purposeful, environmentally-friendly, free of secondary toxicity, residue and pollution, and can keep ecological balance.

The invention discloses application of artemisia apiacea essential oil to pet skin treatment and a preparation process thereof. The preparation process comprises the following steps: (a) picking fresh artemisia apiacea, mint and liquorice, and sequentially chopping to obtain each crushed material for later use; (b) screening the crushed materials obtained in the step (a) in sequence to remove roots and other impurities so as to obtain crushed artemisia apiacea leaves, crushed mint leaves and crushed liquorice leaves in sequence; (c) cleaning and drying the crushed artemisia apiacea leaves, the crushed mint leaves and the crushed liquorice leaves obtained in the step (b), then putting the crushed artemisia apiacea leaves, the crushed mint leaves and the crushed liquorice leaves into a distillation mechanism, and carrying out mixed distillation by adopting a water vapor extraction method to obtain artemisia apiacea mixed essential oil; (d) mixing glycerin monostearate and sodium lauryl sulfate, adding ethanol, heating to 35-37 DEG C, and stirring to fully dissolve the glycerin monostearate and the sodium lauryl sulfate; and (e) adding the artemisia apiacea mixed essential oil obtained in the step (c) into the mixed solution obtained in the step (d), then adding polyvinyl alcohol and glycerol, and uniformly stirring to obtain the artemisia apiacea essential oil. The method prepares artemisia apiacea essential oil suitable for pet skin treatment.

The invention provides an artemisia apiacea essential oil pet deodorization antibacterial liquid and a preparation method thereof. The artemisia apiacea essential oil pet deodorization antibacterial liquid comprises the following components in percentage by weight: 5-15% of artemisia apiacea essential oil, 5-10% of a deodorant, 3-8% of an antibacterial agent and the balance of water, the method comprises the following steps: firstly, collecting an artemisia apiacea stock solution, taking artemisia apiacea leaves, cutting the artemisia apiacea leaves into small pieces, putting the small pieces into a leaf mashing chamber, fully mashing, adding ethanol, stirring, extruding and filtering to obtain the artemisia apiacea stock solution, and flowing into a refining chamber; refining the artemisia apiacea stock solution: adding water into the artemisia apiacea stock solution in the refining chamber, heating and stirring, precipitating for 5-10 minutes after stirring completely, and obtaining supernatant through a suction guide pipe to obtain artemisia apiacea essential oil; and adding a deodorant, an antibacterial agent and water into the prepared artemisia apiacea essential oil in proportion, and fully stirring to obtain the pet deodorization antibacterial liquid containing the artemisia apiacea essential oil.

The invention discloses a microbial environmentally-friendly pesticide for paddy rice, and the microbial environmentally-friendly pesticide comprises the following components by weight: 3-9 parts of shigella dysenteriae bacillus aceticus powder, 5-9 parts of cannabis sativa, 1-3 parts of bdellovibrio powder, 5-8 parts of serrate chloranthus, 5-12 parts of artemisia annua leaf, 1-6 parts of caffeine, 1-10 parts of a synergist, 5-20 parts of dandelion, 4-10 parts of purslane, 6-9 parts of xanthium, 10-15 parts of pomegranate peel, 11-15 parts of folium artemisiae argyi, 12-18 parts of cordate houttuynia, 5-18 parts of herba andrographitis, 20-30 parts of marijuana, 10-20 parts of serrate chloranthus and 100 parts of water. The microbial environmentally-friendly pesticide for paddy rice is a new pesticide which is high-efficiency, purposeful, environmentally-friendly, free of secondary toxicity, residue and pollution, can keep ecological balance, is an important guarantee for realization of ecological agriculture, green ecological agriculture and green enterprise, and has broad prospects.

The invention relates to an ultrasonic extraction method of artemisinin, and belongs to the technical fields of extraction and separation of effective components of traditional Chinese medicines. The method comprises the following steps: putting artemisia apiacea leaf powder extracted by petroleum ether into water bath after pretreatment and petroleum ether extraction, and inserting an ultrasonic probe into the water bath; carrying out ultrasonic extraction under the conditions of 10-30KHz and 50-100W for 8-10 minutes; and extracting while agitating, and then repeatedly extracting for 3-6 times according to the technological parameters, wherein each extraction time is 2-5 minutes. The artemisinin in the artemisia apiacea leaves is extracted by adopting the ultrasonic extraction method, the problems of long extraction time and low extraction rate of an ethanol reflux method, a decocting method and an ion exchange resin method in the prior art are solved, the problems that the product extracted by the existing ultrasonic extraction technology is high in purity, but the overall process is fussy, and high in cost, and consumes a lot of time are also solved, the extraction time is greatly shortened under the premise of ensuring the extraction rate, the technology is simplified, and the cost is reduced.

The invention discloses a method for ultrasonically extracting artemisinin by using a deep eutectic solvent. The method comprises the following steps of: A, pretreating artemisia annua leaves to obtain artemisia annua leaf powder; B, mixing the artemisia annua leaf powder with an aqueous solution of a deep eutectic solvent, and carrying out ultrasonic treatment to obtain a mixed treatment solution, wherein the deep eutectic solvent comprises a hydrogen bond donor and a hydrogen bond acceptor, the hydrogen bond donor is quaternary ammonium salt, and the hydrogen bond acceptor is polyalcohol; and C, carrying out solid-liquid separation on the mixed treatment solution to obtain an artemisia apiacea extracting solution. The deep eutectic solvent formed by quaternary ammonium salt and polyalcohol and artemisinin belong to the same kind or analogues with similar substance components, and the deep eutectic solvent and artemisinin have similar action effects; removal is not needed after the artemisinin is ultrasonically extracted with the deep eutectic solvent, and the structure of the artemisinin cannot be damaged; and therefore, the process can be better simplified, the cost is saved, the extraction efficiency is improved, and the environment is protected.

The invention relates to the technical field of feed manufacturing equipment, in particular to a preparation method and a manufacturing device of artemisia apiacea leaf feed meal. Comprising a supporting frame, a stirring kettle, an embedding strip, a rotating assembly, a connecting block, a stirring assembly, a scraping assembly, a valve and a conveying barrel, the stirring kettle is fixedly connected with the supporting frame, the embedding strip is movably connected with the stirring kettle, the rotating assembly is fixedly connected with the embedding strip, the connecting block is fixedly connected with the rotating assembly, and the stirring assembly is fixedly connected with the connecting block; the scraping assembly comprises a fixed cylinder, a movable rod, a fixed plug and a scraping rod, the fixed cylinder is fixedly connected with the connecting block, one end of the movable rod is slidably connected with the fixed cylinder, the scraping rod is fixedly connected with the other end of the movable rod, raw materials in the stirring kettle are stirred through the stirring assembly, and the scraping rod moves circumferentially along with operation of the rotating assembly; the raw material powder adhered to the inner side wall of the stirring kettle is scraped, so that the preparation personnel can conveniently use the stirring kettle, and the preparation efficiency is improved.

The invention discloses a preparation method for extracting artemisinin from artemisia carvifolia, which comprises the following steps of: 1, crushing artemisia carvifolia leaves to obtain artemisia carvifolia leaf powder; 2, adding the artemisia carvifolia leaf powder into petroleum ether, and continuously extracting for three times; and 3, combining the artemisia carvifolia extracting solutions extracted for three times, filtering, recovering petroleum ether from the filtrate, and concentrating into paste under reduced pressure. The extraction of artemisinin is carried out in an extraction device. A first motor drives a sliding block to move in a storage pool, and then petroleum ether in the storage pool is pressed into an extraction cavity. When the petroleum ether and artemisia carvifolia powder are subjected to an extraction reaction, the artemisia carvifolia powder is stirred by an inner stirring paddle in the extraction cavity, and the petroleum ether solution is stirred by an external stirring paddle. As the stirring directions of the artemisia carvifolia powder and the petroleum ether solution are opposite, the contact area of the petroleum ether and the artemisia carvifolia powder can be effectively increased, the extraction rate of artemisinin is further guaranteed, and waste of resources is avoided.

The invention relates to a nano sustained-release microcapsule as well as a preparation method and application thereof. According to the nano sustained-release microcapsule, an artemisia annua extract, an artemisia apiacea leaf / stem extract, a ganoderma sinense extract, a polygonum multiflorum root extract, a red ginseng extract, a vitex trifolia extract, a gentian root extract, a camphor tree leaf extract and a tea seed extract are taken as capsule core active substances, and sorbitol stearate, cetyl palmitate, lauryl glucoside and water are taken as capsule wall materials. The nano sustained-release micro-capsule is a spherical ultrafine micro-capsule and is composed of a double-layer oil-phase lipid membrane and a water-phase membrane, and the particle size of the nano sustained-release micro-capsule is about 150-300nm. The nano microcapsule can solve the problems of scurf, itching in head and lack of nourishing of scalp, and the nano microcapsule is high in content activity, strong in permeability and lasting in effect.

The invention discloses green pesticide for fruits and vegetables and a preparation method thereof. The green pesticide is prepared from the following raw materials in parts by weight: 1 to 3 parts of rhizoma coptidis, 8 to 10 parts of herba menthae, 4 to 6 parts of a preservative, 15 to 25 parts of cannabis herb, 4 to 6 parts of orange immature fruit, 6 to 8 parts of cynanchum komarovii herb, 15 to 25 parts of camphor, 15 to 25 parts of turpentine oil, 8 to 10 parts of sodium carbonate peroxide, 25 to 35 parts of artemisia annua leaf and 100 to 300 parts of water. The green pesticide is used for insect prevention and killing of the fruits and the vegetables and can replace chemical pesticide; the green pesticide is green and environment-friendly and can promote the growth of plants.

The invention discloses an observation and density measurement method for glandular hairs on Artemisia annua leaf surfaces, comprising the following steps: taking a eugonic Artemisia annua leaf, and feeding the leaf in a centrifuge tube or a microtiter plate; successively adding petroleum ether or normal hexane, absolute ethyl alcohol or carbinol and glacial acetic acid, evenly mixing, and incubating for 5-20min at room temperature; and taking out the leaf, placing the left on a glass slide, adding a drop of double distilled water, covering a cover slip slightly, compacting by a tablet clip, and observing on an optical microscope or calculating the number of the glandular hairs so as to calculate the density of the glandular hairs. The method is simple and convenient, has low requirement on equipment, greatly simplifies the preprocessing procedure of experimental materials, can complete the whole operation within half a hour, uses a little of reagent, is non-toxic, safe and environment-friendly, lowers the measurement cost and time substantially, and can be used for high pass analysis.