Method for extracting artemisinin through enzymatic hydrolysis

An extraction method, artemisinin technology, applied in organic chemistry, bulk chemical production, etc., can solve the problems of cell wall rupture and poor extraction effect, and achieve the effect of high extraction rate, short time consumption, and improved extraction rate

Inactive Publication Date: 2013-09-18
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since artemisinin is almost surrounded by the cell wall composed of cellulose, the traditional extraction method cannot break the cell wall, and there is a huge mass transfer resistance, resulting in poor extraction effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Weigh 250g of Artemisia annua leaf powder into the reaction bottle, add 1L of dilute sulfuric acid solution (pH 6.0) and 2250U of cellulase to it, place it in a constant temperature water bath at 50°C, enzymolyze it for 120min, filter it with suction, and dry the filter cake to constant weight. The filtrate was extracted three times with ether, and the extracts were combined.

[0019] Add 2L ether to the above filter cake to heat and reflux. After reflux for 120min, cool to room temperature, filter with suction, wash the filter cake repeatedly with ether, combine the above extracts, filtrate and washing liquid, and wash with 2% sodium hydroxide solution , and then washed with water until neutral. Concentrate under reduced pressure at 50°C to obtain crude artemisinin, and finally recrystallize with ethanol to obtain 2.07g of fine artemisinin.

Embodiment 2

[0021] Weigh 250g of Artemisia annua leaf powder into the reaction bottle, add 1L of dilute hydrochloric acid solution (pH 4.5) and 3750U of cellulase into it, place it in a constant temperature water bath at 50°C, enzymolyze it for 30min, filter it with suction, and dry the filter cake to constant weight. The filtrate was extracted three times with petroleum ether, and the extracts were combined.

[0022] Add 2L of petroleum ether to the above filter cake to heat and reflux. After reflux for 60min, cool to room temperature and filter with suction. The filter cake is repeatedly washed with petroleum ether. Wash, then wash with water until neutral. Concentrate under reduced pressure at 50°C to obtain crude artemisinin, and finally recrystallize with ethanol to obtain 2.911g of fine artemisinin.

Embodiment 3

[0024] Weigh 250g of Artemisia annua leaf powder into the reaction bottle, add 1L of dilute sulfuric acid solution (pH 3.0) and 3750U of cellulase into it, place in a constant temperature water bath at 50°C, enzymatically hydrolyze for 120min, filter with suction, and dry the filter cake to constant weight. The filtrate was extracted three times with petroleum ether, and the extracts were combined.

[0025] Add 1.5L of petroleum ether to the above filter cake to heat and reflux. After reflux for 30min, cool to room temperature, filter with suction, wash the filter cake repeatedly with ether, combine the above extracts, filtrates and washings, and use 2% sodium hydroxide solution to Wash, then wash with water until neutral. Concentrate under reduced pressure at 50°C to obtain crude artemisinin, and finally recrystallize with ethanol to obtain 2.48g of fine artemisinin.

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Abstract

The invention discloses a method for extracting artemisinin. The method comprises the following steps: crushing leaf of Artemisia carvifolia; carrying out enzymatic hydrolysis in an aqueous solution, and carrying out pumping filtration after the enzymatic hydrolysis; carrying out low temperature extraction of the obtained filter cake by using an organic solvent, and concentrating the obtained extract liquid to obtain crude artemisinin; and recrystallizing the crude artemisinin to obtain purified artemisinin. Compared with traditional extraction methods and supercritical extraction methods, the method adopting an enzymatic hydrolysis-reflux two-step extraction technology in the invention has the advantages of simple process, less impurities, low apparatus requirements, and obviously increased extraction rate, and is an environmentally-friendly and efficient artemisinin extraction method. The method is also suitable for extracting sesquiterpene lactones in other medicinal plants.

Description

technical field [0001] The invention relates to a method for extracting sesquiterpene lactones, in particular to a method for extracting artemisinin from traditional Chinese medicine Artemisia annua. Background technique [0002] Malaria is a global acute parasitic infectious disease caused by Plasmodium, mainly prevalent in tropical and subtropical regions. Worldwide, about 150 million people experience clinical symptoms of malaria each year, and 1 to 3 million people die from malaria infection, most of whom are children and pregnant women with poor immunity to malaria parasites. [0003] Artemisia annua is the whole plant of Artemisia annua L. in Compositae. The artemisinin contained in it is independently developed by my country and recognized by the World Health Organization (WHO) as the only effective treatment for falciparum malaria. drug. my country is the main producing area of ​​Artemisia annua, accounting for more than 90% of the global output. Relying on the reso...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/20
CPCY02P20/54
Inventor 李海波周向东唐小飞欧艳阳刘海林
Owner ARMY MEDICAL UNIV
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