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Method for ultrasonically extracting artemisinin by using deep eutectic solvent

A deep eutectic solvent, artemisinin technology, applied in organic chemistry and other directions, can solve the problems of high extraction cost, easy destruction of active ingredients, environmental pollution, etc., and achieve the effects of improving extraction efficiency, protecting the environment, and simplifying the process

Active Publication Date: 2020-12-25
HARBIN INST OF TECH SHENZHEN GRADUATE SCHOOL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a method for ultrasonically extracting artemisinin using a deep eutectic solvent, aiming at solving the problems of polluted environment, high extraction cost, and low activity in the existing artemisinin acquisition methods. Components are easily damaged during the extraction process, etc.

Method used

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  • Method for ultrasonically extracting artemisinin by using deep eutectic solvent
  • Method for ultrasonically extracting artemisinin by using deep eutectic solvent
  • Method for ultrasonically extracting artemisinin by using deep eutectic solvent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] (1) Preparation of choline chloride-isosorbide deep eutectic solvent (ChCl-Isos DES)

[0048] Weigh 1.396 g of Choline chloride (abbreviated as ChCl) and 2.923 g of isosorbide (abbreviated as Isos), add them into the sample bottle at a molar ratio of 1:2 and mix well. At 50°C, the stirring speed was 500rpm magnetic stirring for 10min; then the ultrasonic power was 1000W, and the ultrasonic temperature was 50°C for ultrasonication for 5min; so circulated until a transparent and clear choline chloride-isosorbide deep eutectic solvent ( ChCl-Isos DES).

[0049] (2) Characterization of physicochemical properties of choline chloride-isosorbide deep eutectic solvent (ChCl-Isos DES)

[0050] Measured ChCl, Isos, ChCl-Isos DES Fourier transform infrared spectrum (FTIR) contrast such as figure 2 Shown, known, ChCl-Isos DES chemical bond position and chemical group are all different with ChCl, Isos; Use DMSO as deuterated solvent, record the proton nuclear magnetic resonance s...

Embodiment 2

[0056] Example 2 Ultrasonic extraction of artemisinin using ChCl-Isos DES (molar ratio is 1:2)

[0057] (1) Take the oil of Artemisia annua, remove the branches, take the leaves and place them in a blast drying oven at 60°C to air-dry until there is no water residue in the leaves of Artemisia annua, crush and grind them with a mortar, and pass through a 120-mesh sieve to obtain 120-mesh yellow flowers Artemisia leaf powder for later use.

[0058] (2) Mix 0.1g of Artemisia annua leaf powder with 2mL of ChCl-Isos DES (1:2) aqueous solution (the ratio of ChCl-Isos DES (1:2) to water is 0.6g:1mL), and the input power of ultrasound is 1000W, ultrasonic frequency 40kHz, ultrasonic at 45°C for 30min to obtain a mixed treatment solution.

[0059] (3) The mixed treatment solution was centrifuged at 1300 rpm for 6 minutes to obtain an artemisinin extract.

[0060] (4) Take 1 mL of artemisinin extract, add 0.2 wt% NaOH solution, react in 50°C water bath for 30 min, cool to room tempera...

Embodiment 3

[0061] Example 3 Test the ChCl-Isos DES formed by different molar ratios of ChCl and Isos on the extraction effect of artemisinin

[0062] (1) Prepare two ChCl-Isos DES with different molar ratios of ChCl and Isos (1:4, 1:6)

[0063] The preparation steps are the same as the step (1) of Example 1, except that ChCl and Isos are mixed in a molar ratio of 1:4 and 1:6 respectively, and the obtained deep eutectic solvent is respectively denoted as ChCl-Isos DES (1 :4), ChCl-Isos DES (1:6), the deep eutectic solvent that embodiment 1 obtains is denoted as ChCl-Isos DES (1:2).

[0064] (2) The ChCl-Isos DES prepared by different molar ratios of ChCl and Isos is tested for the extraction performance of artemisinin

[0065] (2.1) The extraction steps of artemisinin were the same as in Example 2, replacing ChCl-Isos DES (1:2) with ChCl-Isos DES (1:4) and ChCl-Isos DES (1:6) respectively.

[0066] (2.2) The determination steps of the extraction efficiency of artemisinin are the same as...

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Abstract

The invention discloses a method for ultrasonically extracting artemisinin by using a deep eutectic solvent. The method comprises the following steps of: A, pretreating artemisia annua leaves to obtain artemisia annua leaf powder; B, mixing the artemisia annua leaf powder with an aqueous solution of a deep eutectic solvent, and carrying out ultrasonic treatment to obtain a mixed treatment solution, wherein the deep eutectic solvent comprises a hydrogen bond donor and a hydrogen bond acceptor, the hydrogen bond donor is quaternary ammonium salt, and the hydrogen bond acceptor is polyalcohol; and C, carrying out solid-liquid separation on the mixed treatment solution to obtain an artemisia apiacea extracting solution. The deep eutectic solvent formed by quaternary ammonium salt and polyalcohol and artemisinin belong to the same kind or analogues with similar substance components, and the deep eutectic solvent and artemisinin have similar action effects; removal is not needed after the artemisinin is ultrasonically extracted with the deep eutectic solvent, and the structure of the artemisinin cannot be damaged; and therefore, the process can be better simplified, the cost is saved, the extraction efficiency is improved, and the environment is protected.

Description

technical field [0001] The invention relates to the technical field of deep processing of natural products, in particular to a method for ultrasonically extracting artemisinin with a deep eutectic solvent. Background technique [0002] artemisinin It is the drug with the best effect in the treatment of malaria drug resistance, and the combination therapy based on artemisinin drugs is also the most effective and important means of treating malaria at present. Artemisinin is a colorless needle-like crystal, easily soluble in chloroform, acetone, ethyl acetate and benzene, soluble in ethanol and ether, slightly soluble in cold petroleum ether, and almost insoluble in water. Because of its special peroxy group, it is unstable to heat and is easily decomposed by moisture, heat and reducing substances. [0003] At present, the methods for obtaining artemisinin mainly include chemical synthesis, biosynthesis, extraction and purification. Among them, the chemical synthesis is ma...

Claims

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Application Information

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IPC IPC(8): C07D493/20
CPCC07D493/20
Inventor 张嘉恒潘祖辰梁一红薄怡扬卢贝贝李远彬易明杰王乐明
Owner HARBIN INST OF TECH SHENZHEN GRADUATE SCHOOL
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