The invention provides a neem oil nanometer composition, and belongs to the field of cosmetic. The neem oil nanometer composition comprises, by weight, 1.0 to 30.0% of neem oil, 25.0 to 75.0% of an emulsifier, 0.8 to 17.0% of a co-emulsifier, 1.0 to 65.0% of a polyhydric alcohol, 0.01 to 2.0% of an anti-oxidant, and 0.01 to 4.0% of an antiseptic. According to a preparation method, a homogeneous disperse system with thermodynamic stability is formed by neem oil, the emulsifier, the co-emulsifier, and the polyhydric alcohol, water solubility is high, stability is high, no floating oil is generated, no aggregation is caused, and excellent mosquito repelling, bacteria inhibiting, inflammation relieving, and pain eliminating effects are achieved. The neem oil nanometer composition is high in drug loading capacity, is capable of entering skin deep tissue through skin stratum corneum, releasing neem oil continuously, and achieving excellent mosquito repelling, bacteria inhibiting, inflammation relieving, and pain eliminating effects.