A kind of aatsw1 gene promoter and its application and preparation method

A promoter and gene technology, applied in the field of genetic engineering, can solve problems such as harm and normal plant growth burden

Active Publication Date: 2017-12-29
上海阿提密斯生物科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This promoter can drive the expression of foreign genes in all tissues and organs, so it is likely to cause a certain burden and harm to the normal growth of plants

Method used

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  • A kind of aatsw1 gene promoter and its application and preparation method
  • A kind of aatsw1 gene promoter and its application and preparation method
  • A kind of aatsw1 gene promoter and its application and preparation method

Examples

Experimental program
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Embodiment Construction

[0031] The specific implementation manners provided by the present invention will be described in detail below with reference to the accompanying drawings.

[0032] The experimental methods without specific conditions in the following examples usually follow conventional conditions, such as molecular cloning such as Sambrook: For laboratory manuals, see the conditions described in the 1989 edition of New York: Cold Spring Harbor Laboratory Press, or according to the manufacturer The suggested conditions.

[0033] The Agrobacterium tumefaciens EHA105 involved in this example has been published in "Huang Yali, Jiang Xiliang, Tian Yunlong, Guo Ping, Zhu Changxiong; Agrobacterium tumefaciens-mediated genetic transformation of Trichoderma harzianum, Chinese Journal of Biological Engineering, 2008, 28 ( 3): "38-43" published in the literature. Agrobacterium tumefaciens EHA105, plasmid pCAMBIA1391Z can be obtained through publicly available commercial channels, such as from Australia CAM...

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Abstract

The invention discloses an AaTSW1 gene promoter and a preparation method thereof. A nucleotide sequence of the promoter is shown as a first sequence in a sequence table. Meanwhile, the invention further relates to application of the AaTSW1 gene promoter in the genetic engineering breeding in which metabolic products are expressed and produced by means of glandular hair tissue of a plant. According to the AaTSW1 gene promoter and the application and preparation method thereof, the promoter can guide genes to be specifically expressed in a glandular hair of the plant, and due to the fact that the promoter which is specifically expressed in the glandular hair performs genetic manipulation on a glandular hair system of the plant, harm cannot be caused to the growth and development of the plant; accordingly, the important significance is achieved for genetic engineering breeding in which the metabolic products are expressed and produced by means of the glandular hair tissue of the plant.

Description

Technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a gene promoter and its application and preparation method. Background technique [0002] Artemisia annua (Artemisia annua L.) is an annual herb of the genus Artemisia in the family Compositae. Artemisinin is a sesquiterpene lactone compound containing a peroxy bridge structure isolated from its aerial part. It is currently recognized as the most effective treatment for malaria in the world, especially for cerebral malaria and anti-chloroquine malaria. effective. Currently, the most effective treatment for malaria recommended by the World Health Organization is artemisinin combination therapy (ACTs). At present, the main source of artemisinin is extracted from the aerial part of the Artemisia annua plant. However, the content of artemisinin in Artemisia annua is very low (0.01%-1%), which limits the large-scale commercial production of this drug. [0003] AaTSW1 is a WRKY...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/84C12N1/21C12N15/10A01H5/00C12R1/01
Inventor 唐克轩陈明慧颜廷祥付雪晴石璞马亚男郝小龙孙小芬
Owner 上海阿提密斯生物科技发展有限公司
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