Glandular hairs of Artemisia annua dominantly express aatcp14 gene promoter and its obtaining method and application

Abstract

The invention discloses an artemisia annua L. glandular trichome preferential expression AaTCP14 gene promoter. The artemisia annua L. glandular trichome preferential expression AaTCP14 gene promotercontains nucleotide sequences which are shown as SEQ ID NO:1 or complementary to those shown in the SEQ ID NO:1. The artemisia annua L. glandular trichome preferential expression AaTCP14 gene promotercan regulate expression of target genes in plant tissues, particularly preferential expression in glandular secretory trichome and T shape trichome. Meanwhile, the invention also relates to an acquisition method of the artemisia annua L. glandular trichome preferential expression AaTCP14 gene promoter and application of the artemisia annua L. glandular trichome preferential expression AaTCP14 gene promoter to genetic engineering breeding which applies plant glandular trichome tissues to expressing and producing metabolic products. Due to the fact that the glandular trichome preferential expression gene promoter has no damage to growth of plants when applied to genetic operation on glandular trichome systems of the plants, the artemisia annua L. glandular trichome preferential expression AaTCP14 gene promoter is significant to genetic engineering breeding which expresses and produces metabolic products in plant tissues, especially in the glandular trichome.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and relates to an AaTCP14 gene promoter expressing predominantly in glandular hairs of Artemisia annua and its obtaining method and application. Specifically, it is an AaTCP14 gene promoter (proTCP14) that is predominantly expressed in various plant tissues, especially secretory and non-secretive glandular trichomes, obtained by means of molecular biology, and its application in transgenic plants is analyzed. Background technique [0002] Artemisia annua L. is an annual herb of the genus Artemisia in the Asteraceae family. The plant has a strong volatile aroma and contains many secondary metabolites: artemisinin, artemisinone, β-caryophyllene, β- Farnesene, germanene, α-pinene, camphor, volatile oil, etc. Artemisinin (Artemisinin) is a sesquiterpene lactone compound containing a peroxo bridge structure isolated from the leaves of Artemisia annua. , the main active ingredient of ACTs). In rec...

AI Technical Summary

Problems solved by technology

In the current genetic engineering research of Artemisia annua, constitutive promoters are often used, such as the cauliflower mosaic virus 35S promoter (CaMV35S), which can drive the expression of foreign genes in all tissues and organs, and will cause excessive consumption. The substance and energy in the cell, and the expression of the target gene cannot be regulated in time and space, which is likely to cause a certain burden and harm to the normal growth of plants

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