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Lignocellulos substance high-efficiency degrading bacterium S1 and application thereof

A technology of lignocellulose-based, high-efficiency degrading bacteria, which is applied in the treatment of bacteria, microorganisms, and biological organic parts, etc., can solve the problems of low enzyme activity, and achieve stable enzyme production and enzyme activity, wide application, and obvious lignin degradation. capacity and effect of hemicellulose degradability

Active Publication Date: 2016-03-09
北京中农国泰科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

This patented technology allows for rapid multiplication of bacilluses called enterobacters (Escherichia colϊ) which are able to break down cell walls or other substances found inside them. These Escholars produce special proteases made up mostly of small molecules like amino acids and sugars while they use these materials as food sources during their metabolism process. They also have high temperature-resistance properties making them ideal candidates for applications such as bioplastic reservoir construction. Overall this new method provides an efficient way to make certain types of organisms better suited for various purposes including industrial uses.

Problems solved by technology

Technologies aimed at developing an improved process called biofuel conversion (BFC) involve converting wastes containing carbohydrate molecules like starch and hemiculancies into useful compounds. Bifidobacillum sporium is currently being studied due to their ability to break down various forms of plant fibers while producing different metabolites. These techniques help recycle these valuable sources more efficiently than other alternatives.

Method used

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  • Lignocellulos substance high-efficiency degrading bacterium S1 and application thereof
  • Lignocellulos substance high-efficiency degrading bacterium S1 and application thereof
  • Lignocellulos substance high-efficiency degrading bacterium S1 and application thereof

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specific Embodiment approach 1

[0024] Specific embodiment 1: A lignocellulosic material efficient degrading bacterium S1 of the present embodiment, it is Enterobacterhormaechei (Enterobacterhormaechei) S1, preserved in China Microorganism Culture Collection Administration General Microorganism Center, preservation address is Beijing No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, the preservation date is December 12, 2014, and the preservation number is CGMCCNo.10165.

[0025] Enterobacterhormaechei (Enterobacterhormaechei) S1 of the present embodiment is a Gram-negative bacterium, and the bacterial cell shape of the strain is rod-shaped, and the cell size is 0.55-0.58×1.4-1.5 μm, does not form spores, and has no flagella and capsule ; On LB medium, round, opaque, milky white, with smooth protrusions and neat edges are formed (such as figure 1 shown). The results of the physiological and biochemical reactions of the strain included 18 items and 28 indicators; combining the results of morpholo...

specific Embodiment approach 2

[0094] Embodiment 2: The application of a lignocellulosic substance-degrading bacteria S1 in this embodiment is used for degrading lignocellulosic substances.

[0095] Bacterial strain of the present invention is carried out following functional detection:

[0096] Enterobacterhormaechei (Enterobacterhormaechei) S1 of the specific embodiment was tested for weight loss rate and lignin, cellulose and hemicellulose degradation of bagged fungus waste and corn stalks to verify its unique functions. details as follows:

[0097] 1. Determination of weight loss rate of bagged fungus waste and corn stalks

[0098] 1.1 Corn stalk powder

[0099] Corn stalks were taken from the experimental field of the laboratory in Hulan Campus, Heilongjiang University, Harbin City, Heilongjiang Province in October 2012, dried, crushed and passed through a 40-mesh sieve, and set aside.

[0100] 1.2 bags of fungus waste

[0101] Bags of fungus waste were provided by Mudanjiang Branch of Heilongjiang...

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Abstract

The invention relates to a lignocellulos substance high-efficiency degrading bacterium S1 and application thereof. The lignocellulos substance high-efficiency degrading bacterium S1 is Enterobacter hormaechei S1 and was preserved with preservation number CGMCC No.10165 on December 12, 2014 by China General Microbiological Culture Collection Center. The bacterium is used for degrading lignocellulos substances. The strain provided by the invention has the advantages of quick proliferation, strong applicability and wide application; a weight-loss ratio of bagged agaric wastes after being fermented by Enterobacter hormaechei S1 is above 31 percent and a weight-loss ratio of corn stalks after being fermented by Enterobacter hormaechei S1 is above 33 percent.

Description

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Claims

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Application Information

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Owner 北京中农国泰科技有限公司
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