A Simple Detection Method of Alkaline Phosphatase Activity
A technology of phosphatase activity and phosphatase, which is applied in the detection field of alkaline phosphatase activity, can solve the problems of high analysis cost, bulky size, and expensive instrument, achieve broad application prospects, reduce analysis cost, and analyze the process simply and easily line effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0019] Use a simple and low-cost alkaline phosphatase activity detection method to detect 5U / mL alkaline phosphatase sample solution and blank sample (buffer solution without analyte).
[0020] Such as figure 1 As shown, the specific implementation process is as follows: step 1, add 15 μ L 5U / mL alkaline phosphatase sample solution (prepared by 10 mM tris-HCl buffer solution containing 100 mM NaCl, pH 7.4) in a 1.5 mL plastic detection test tube, and then add 15 μL of 200 μM pyrophosphate solution (prepared from 10 mM tris-HCl buffer solution containing 100 mM NaCl, pH 7.4) was mixed with it, and placed at 37 ° C for 60 min, and continued to add 15 μ L of 50 μM copper nitrate aqueous solution, reacted at room temperature for 15 min, and then continued Add 15 μL of 1 mg / mL glucoamylase solution (prepared from 20 mM acetic acid-sodium acetate buffer solution, pH 4.5), react at room temperature for 70 min, then add 15 μL of 2% soluble starch solution (heated by ultrapure water wi...
Embodiment 2
[0023] A simple and low-cost alkaline phosphatase activity detection method is used to detect the alkaline phosphatase sample solution with an activity concentration ranging from 0.0375 to 5 U / mL.
[0024] Such as figure 1 As shown, the concrete steps of detecting each alkaline phosphatase sample solution in the present embodiment are: Step 1, add 15 μ L of alkaline phosphatase sample solution (containing 100mMNaCl 10mM tris-HCl buffer solution, pH 7.4), then add 15μL 200μM pyrophosphate solution (prepared from 10mM tris-HCl buffer solution containing 100mM NaCl, pH 7.4) to mix with it, and place it at 37℃ for 60min, Continue to add 15 μL of 50 μM copper nitrate aqueous solution, react at room temperature for 15 minutes, then continue to add 15 μL of 1 mg / mL glucoamylase solution (prepared from 20 mM acetic acid-sodium acetate buffer solution, pH 4.5), react at room temperature for 70 minutes, and then add 15 μL of 2% soluble starch Solution (prepared by heating ultrapure wat...
PUM
Login to view more Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap