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Method for extracting chenodeoxycholic acid from duck bile

A technology of chenodeoxycholic acid and duck bile, which is applied in the field of extracting chenodeoxycholic acid, can solve the problem of no profit, and achieve the effect of reducing the generation of waste water and saving costs

Active Publication Date: 2017-12-22
眉山市新功生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a result, enterprises that use chicken bile as raw material to produce chenodeoxycholic acid basically have little profit to make

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for extracting chenodeoxycholic acid from duck bile, comprising the steps of:

[0032] Step 1, the dissociation of bound chenodeoxycholic acid

[0033] 1.1 Put 1000mL of duck bile into the reaction bottle, add 50g of sodium hydroxide, stir to dissolve, raise the temperature to 100°C, and keep the temperature for reaction for 18-24 hours; break the bond of taurochenodeoxycholic acid ester and convert it into chenodeoxy Sodium bile acid;

[0034] 1.2 After the reaction is completed, lower the temperature to 10-15°C, slowly add dilute hydrochloric acid or dilute sulfuric acid dropwise, and adjust the pH value to 3-4. At this time, bile acids are precipitated, and the waste water is removed by filtration, and the solid is collected to obtain goose Deoxycholic acid crude product 192.6g.

[0035] In this step, since duck bile mainly contains 3α, 7α-dihydroxy-5β-cholic acid (commonly known as chenodeoxycholic acid) and 3α, 7α, 23-trihydroxy-5β-cholanic acid, they ar...

Embodiment 2

[0046] A method for extracting chenodeoxycholic acid from duck bile, comprising the steps of:

[0047] Step 1, the dissociation of bound chenodeoxycholic acid

[0048]1.1 Put 1000mL of duck bile into the reaction bottle, add 60g of sodium hydroxide, stir to dissolve, raise the temperature to 110°C, and keep the temperature for reaction for 18-24 hours; break the bond of taurochenodeoxycholic acid ester and convert it into chenodeoxy Sodium bile acid;

[0049] 1.2 After the reaction is completed, lower the temperature to 10-15°C, slowly add dilute hydrochloric acid or dilute sulfuric acid dropwise, and adjust the pH value to 3-4. At this time, bile acids are precipitated, and the waste water is removed by filtration, and the solid is collected to obtain goose Deoxycholic acid crude product 190.3g.

[0050] In this step, since duck bile mainly contains 3α, 7α-dihydroxy-5β-cholic acid (commonly known as chenodeoxycholic acid) and 3α, 7α, 23-trihydroxy-5β-cholanic acid, they are...

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PUM

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Abstract

The invention discloses a method for extracting chenodeoxycholic acid from duck bile. The method comprises the following steps of 1, dissociating the combined state chenodeoxycholic acid; 2, removing fatty acid from a crude product; 3, removing 3alpha, 7alpha, 23-trihydroxy-5beta-cholanic acid; 4, refining the chenodeoxycholic acid. Through simple raw materials and steps, the higher-purity chenodeoxycholic acid can be obtained. Only petroleum ether is used in the method; the removal of fatty acid can be completed; the conventional methanol dissolution or water addition is omitted; the waste water generation is reduced. In addition, through regulating the sequential order of water addition and temperature reduction, the 3alpha, 7alpha, 23-trihydroxy-5beta-cholanic acid can be effectively eliminated; the cost is reduced. In addition, acetonitrile and chloroform with high toxicity is replaced by using dichloromethane; great advantages are realized on the production.

Description

technical field [0001] The invention relates to a method for extracting chemical substances, in particular to a method for extracting chenodeoxycholic acid from duck bile. Background technique [0002] The chemical name of chenodeoxycholic acid (CDCA) is 3α, 7α-dihydroxy-5β-cholic acid, and its molecular formula is C 24 h 40 o 4 , molecular weight 392.58. It was first extracted from goose bile in 1848. Chenodeoxycholic acid has the ability to dissolve gallstones and other pharmaceutical functions. Since the 1970s, it has been clinically used as a drug for the treatment of gallstones and other hepatobiliary diseases. Recent studies have found that ursodeoxycholic acid, an isomer of chenodeoxycholic acid, has a better clinical effect in dissolving gallstones, and has no side effects. Therefore, ursodeoxycholic acid has gradually replaced chenodeoxycholic acid in the clinical application of gallstone-dissolving drugs. [0003] Natural ursodeoxycholic acid is derived from ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J9/00
CPCC07J9/005
Inventor 梁万超蒋先曲
Owner 眉山市新功生物科技有限公司