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Probe, primer and kit for detecting non-deletion α-thalassemia gene mutation

A non-deletion type, kit-based technology, applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve the problems of long detection time, few sites, low sensitivity, etc.

Active Publication Date: 2021-07-06
亚能生物技术(深圳)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] In view of the above, the technical problem solved by the present invention is: to provide a probe, primer and kit for detecting the mutation of non-deletion α-thalassemia gene, which can solve the above-mentioned problems of long detection time, few sites and low sensitivity

Method used

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  • Probe, primer and kit for detecting non-deletion α-thalassemia gene mutation
  • Probe, primer and kit for detecting non-deletion α-thalassemia gene mutation
  • Probe, primer and kit for detecting non-deletion α-thalassemia gene mutation

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Experimental program
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Effect test

Embodiment

[0098] The kit of this embodiment is used for whole blood genomic DNA in premarital, prenatal and prenatal screening samples of clinical patients with thalassemia, and can qualitatively detect thalassemia genotypes with a high detection rate in the Chinese population, including 7 non- Deleted α-thalassaemia (α CS α / , α WS α / , α QS α / , α 59 α / , α 30 α / , α 31 α / and α 13 α / ).

[0099] 【Principle of inspection】

[0100] This kit is based on fluorescent PCR amplification and melting curve analysis.

[0101] Design specific PCR primers to amplify a DNA fragment of a certain length, which contains the genotype to be detected. Melting curve analysis was performed after PCR amplification.

[0102] The genotype of the template was judged according to the size of the melting point and the shape of the melting peaks in different channels.

[0103] 【Main components】

[0104] 1. The main components of the kit are shown in Table 5 below.

[0105] table 5

[0106]

[0107] Not...

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Abstract

The present invention relates to a probe, primer and kit for detecting non-deletion type α-thalassemia gene mutation, wherein the probe for detecting non-deletion type α-thalassemia gene mutation comprises: WS and alpha QS Probe αα T ‑P1; used to detect α CS Probe αα T ‑P2; Probe αα for detection of CD30 and CD31 loci T ‑P3; probe αα for detection of CD13 locus T ‑P4; Probe αα for detection of CD59 locus T -P5. The invention can simultaneously detect seven kinds of non-deletion α-thalassemia gene mutations at one time. Compared with similar products, the detection site is more comprehensive, the detection time is shorter, and the efficiency is higher.

Description

technical field [0001] The invention relates to gene detection technology, in particular to a probe, primer and kit for detecting non-deletion type α-thalassemia gene mutation. Background technique [0002] The human α-globin gene cluster is located on chromosome 16, expressing α-globin chains combined with β-globin chains to form functional hemoglobin tetramers. Under normal circumstances, the ratio of α- and β-globin chains expressed by the human globin gene is appropriate (1:1). When the α-globin gene is defective, the synthesis of α-chain is reduced and the β-chain is relatively excess, which can lead to α- Thalassemia disease. α-thalassemia includes deletion α-thalassemia and non-deletion α-thalassemia. [0003] For non-deletion α-thalassaemia, most of its gene mutations occur in one gene (α2 or α1), which will not impair the function of the other α-gene, so it is defined as α + - thalassemia, which is alpha T α / or αα T / . The heterozygotes have both static α-thal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 曲玲陈永娟田洁
Owner 亚能生物技术(深圳)有限公司
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