Jelly-like bacillus, microbial fertilizer, preparation method and application thereof

Abstract

The invention provides a jelly-like bacillus whose preservation number is CGMCC No.16005. The present invention also provides a microbial fertilizer, comprising the jelly-like bacillus described in the above technical solution, a carrier and an organic fertilizer, wherein the jelly-like bacillus described in the above technical solution is compounded on the carrier. The jelly-like bacillus provided by the present invention has strong decomposition ability to potassium-containing silicate ores such as mica and feldspar in the soil, and when applied in agricultural production, it can not only play a role in dissolving potassium and phosphorus, but also easily form a root system Dominant species, used as microbial fertilizer for fruits, vegetables and crops can enhance the cold resistance, drought resistance, disease resistance and stress resistance of crops and flowers, and the phenomenon of small leaves, yellow leaves and early leaf fall is significantly reduced.

Description

technical field

[0001] The invention belongs to the technical field of microbial fertilizers, and in particular relates to jelly-like bacillus, microbial fertilizers, a preparation method and applications thereof. Background technique

[0002] Due to the excessive use of inorganic fertilizers, the soil quality of flowers in my country is generally poor at present, especially in the low carbon-nitrogen ratio of the soil, which leads to the destruction of the soil microbial system and the continuous reduction of beneficial microorganisms, which seriously affects the growth and development of the root system.

[0003] Over the years, the application of chemical fertilizers has played a key role in increasing agricultural production and income. According to data, China's potash fertilizer production accounts for only 0.3% of the world's total, while its consumption reaches 14.7%. Although it imports more than 2 million tons of potash fertilizer every year, it still cannot meet t...

Examples

Embodiment 1

[0045] (1) Activate the strain in the cryopreservation tube with the preservation number CGMCC No.16005 in a slant (37°C for 24h), and purify it in a plate (37°C for 24h), and finally obtain the slant strain or strain slant , cultured at 30°C for 2 days, the surface of the slant turned white, after smearing, observed under a microscope, all the bacteria became spores, and stored in a refrigerator at 4°C; wherein, the composition of the slant medium was (g / L): yeast powder 7.0, Yeast extract 0.7, protein jelly 10.0, dextrin 2.0, disodium hydrogen phosphate 1.0, zinc sulfate 0.2, calcium sulfate 0.2, agar 20.0.

[0046] (2) Shake flask culture stage: take a ring of purified bacteria, insert it into a 250mL Erlenmeyer flask with a capacity of 50mL of seed medium, sterilize at 121°C for 0.5h, cool to room temperature, and inoculate jelly-like spore-like spores Bacteria, cultured on a constant temperature shaking table, culture conditions: temperature 30°C, rotation speed 200r / min,...

Embodiment 2

[0062] 1.1 Materials

[0063] 1.1.1 Test fertilizer: the microbial fertilizer of Example 1, granule, 20 kg / bag; bacteria content ≥ 2.0×10 8 cfu / g, water content ≤10%;

[0064] 1.1.2 Tested crops: soybean variety Jindou 20.

[0065] 1.2 Method

[0066] T: microbial fertilizer, 40kg / mu basal application;

[0067] CK: The conventional control is 5m of farmyard manure per mu 3 , basal application of nitrogen fertilizer (N) 15kg, phosphorus fertilizer (P 2 o 5 )6.0kg, potassium fertilizer (K 2 O) 10.0kg. There are 15,800 seedlings per mu. Planted on April 21, harvested on October 14, each district received a single harvest.

[0068] 2 Results and Analysis

[0069] 2.1 Effects of different treatments on biological characters of soybean

[0070] Table 1 Effect of microbial fertilizer on biological characters of soybean

[0071]

[0072] 2.2 Effects of different treatments on soybean yield

[0073] Table 2 Effect of microbial fertilizer on soybean yield

[0074]

...

Embodiment 3

[0077] Adopt simple comparative test to carry out field test of Lycium barbarum, 40 kilograms of microbial bacterial fertilizers of mu application embodiment 1, verify that this bacterial agent has the effect of improving soil fertilizer utilization rate, increasing output.

[0078] 1. Materials and methods

[0079] 1.1 Test soil

[0080] The test site is the No. 1 Production Brigade of Nuomhong Farm, Dulan County, Haixi Mongolian and Tibetan Autonomous Prefecture, Qinghai Province. The sandy loam soil has medium soil fertility. The soil organic matter content is 17.0mg / kg, hydrolyzable nitrogen is 119mg / kg, and available phosphorus is 114mg. / kg, available potassium 232mg / kg, pH 8.3. uniform soil fertility,

[0081] 1.2 Test fertilizers

[0082] The microbial bacterial fertilizer of Example 1, granules, 20 kg / bag; bacteria content ≥ 2.0 × 108 cfu / g, water content ≤ 10%.

[0083] 1.3 Test varieties

[0084] The variety of wolfberry tested was Ningqi No. 7.

[0085] 1.4 T...

technical field

[0001] The invention belongs to the technical field of microbial fertilizers, and in particular relates to jelly-like bacillus, microbial fertilizers, a preparation method and applications thereof. Background technique

[0002] Due to the excessive use of inorganic fertilizers, the soil quality of flowers in my country is generally poor at present, especially in the low carbon-nitrogen ratio of the soil, which leads to the destruction of the soil microbial system and the continuous reduction of beneficial microorganisms, which seriously affects the growth and development of the root system.

[0003] Over the years, the application of chemical fertilizers has played a key role in increasing agricultural production and income. According to data, China's potash fertilizer production accounts for only 0.3% of the world's total, while its consumption reaches 14.7%. Although it imports more than 2 million tons of potash fertilizer every year, it still cannot meet t...

Examples

Embodiment 1

[0045] (1) Activate the strain in the cryopreservation tube with the preservation number CGMCC No.16005 in a slant (37°C for 24h), and purify it in a plate (37°C for 24h), and finally obtain the slant strain or strain slant , cultured at 30°C for 2 days, the surface of the slant turned white, after smearing, observed under a microscope, all the bacteria became spores, and stored in a refrigerator at 4°C; wherein, the composition of the slant medium was (g / L): yeast powder 7.0, Yeast extract 0.7, protein jelly 10.0, dextrin 2.0, disodium hydrogen phosphate 1.0, zinc sulfate 0.2, calcium sulfate 0.2, agar 20.0.

[0046] (2) Shake flask culture stage: take a ring of purified bacteria, insert it into a 250mL Erlenmeyer flask with a capacity of 50mL of seed medium, sterilize at 121°C for 0.5h, cool to room temperature, and inoculate jelly-like spore-like spores Bacteria, cultured on a constant temperature shaking table, culture conditions: temperature 30°C, rotation speed 200r / min,...

Embodiment 2

[0062] 1.1 Materials

[0063] 1.1.1 Test fertilizer: the microbial fertilizer of Example 1, granule, 20 kg / bag; bacteria content ≥ 2.0×10 8 cfu / g, water content ≤10%;

[0064] 1.1.2 Tested crops: soybean variety Jindou 20.

[0065] 1.2 Method

[0066] T: microbial fertilizer, 40kg / mu basal application;

[0067] CK: The conventional control is 5m of farmyard manure per mu 3 , basal application of nitrogen fertilizer (N) 15kg, phosphorus fertilizer (P 2 o 5 )6.0kg, potassium fertilizer (K 2 O) 10.0kg. There are 15,800 seedlings per mu. Planted on April 21, harvested on October 14, each district received a single harvest.

[0068] 2 Results and Analysis

[0069] 2.1 Effects of different treatments on biological characters of soybean

[0070] Table 1 Effect of microbial fertilizer on biological characters of soybean

[0071]

[0072] 2.2 Effects of different treatments on soybean yield

[0073] Table 2 Effect of microbial fertilizer on soybean yield

[0074]

...

Embodiment 3

[0077] Adopt simple comparative test to carry out field test of Lycium barbarum, 40 kilograms of microbial bacterial fertilizers of mu application embodiment 1, verify that this bacterial agent has the effect of improving soil fertilizer utilization rate, increasing output.

[0078] 1. Materials and methods

[0079] 1.1 Test soil

[0080] The test site is the No. 1 Production Brigade of Nuomhong Farm, Dulan County, Haixi Mongolian and Tibetan Autonomous Prefecture, Qinghai Province. The sandy loam soil has medium soil fertility. The soil organic matter content is 17.0mg / kg, hydrolyzable nitrogen is 119mg / kg, and available phosphorus is 114mg. / kg, available potassium 232mg / kg, pH 8.3. uniform soil fertility,

[0081] 1.2 Test fertilizers

[0082] The microbial bacterial fertilizer of Example 1, granules, 20 kg / bag; bacteria content ≥ 2.0 × 108 cfu / g, water content ≤ 10%.

[0083] 1.3 Test varieties

[0084] The variety of wolfberry tested was Ningqi No. 7.

[0085] 1.4 T...

Images