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Sample processing device and biological activity screening system containing the device

A planar chromatography and microplate technology, applied in the field of drug analysis and biological testing, can solve the problems of difficult implementation and difficult to determine the chemical composition of biological activity, and achieve the effect of ensuring scalability.

Active Publication Date: 2022-02-22
GUANGDONG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to make each chromatographic spot fall into the same microwell pool as much as possible, this method requires that the planar chromatographic spots correspond strictly to the microwell plate array, which is difficult to implement for thin-layer chromatograms with irregular spot shapes and positions; For the case where a chromatographic spot falls into multiple microporous pools and there are multiple co-existing components in the microporous pools, it is difficult to determine the chemical composition of the biological activity

Method used

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  • Sample processing device and biological activity screening system containing the device
  • Sample processing device and biological activity screening system containing the device
  • Sample processing device and biological activity screening system containing the device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0142] A sample processing device

[0143] In this embodiment, the grid plate corresponding to the array format of the 384-well square-mouth microplate is taken as an example to illustrate the structure of the grid plate, which can be adjusted according to the array format of the microplate during practical application.

[0144] Such as image 3 The grid plate shown has a plurality of channel-type spaces (that is, the inner space of each grid) defined by the grid frame, and the channel-type space has an open first end and a second end, wherein on the open plane of the first end, the area of ​​the grid frame defining each channel-shaped space is 10% of the area of ​​the first end of the channel-shaped space. At this time, the frame thickness of each grid frame at the first end is narrow enough to form a sharp edge, which can be used to cut the chromatographic thin layers of the planar chromatogram, ensuring that all the chromatographic thin layers can be cut and separated. Th...

Embodiment 2

[0147] Embodiment 2 A device for preparing planar chromatographic microarray

[0148] The device contains a plane chromatogram, a grid plate, a microporous filter plate, and a microporous plate corresponding to each other in an array format. And a biological activity detection unit and a mass spectrometry analysis unit.

[0149] The planar chromatography is used to construct the planar chromatographic fingerprint of the sample to be screened; the grid plate is used to divide and peel off the chromatographic thin layer of the planar chromatographic fingerprint according to the microplate array format; the microporous filter plate is used to divide the grid interface and The stripped chromatographic thin layer is positioned and eluted to the micro-orifice plate; the micro-orifice plate is used to position and receive the components of the plane chromatogram, and prepare a microarray sample of the plane chromatogram components.

[0150] In this embodiment, the chromatographic sp...

Embodiment 3

[0152] Embodiment 3 A kind of biological activity screening system

[0153] Such as Figure 13 The biological activity screening system shown includes a one-to-one array format of planar chromatography, grid plate, microporous filter plate, micropore plate, biological activity detection unit and mass spectrometry analysis unit.

[0154] The planar chromatography is used to construct the planar chromatographic fingerprint of the sample to be screened; the grid plate is used to divide and peel off the chromatographic thin layer of the planar chromatographic fingerprint according to the microplate array format; the microporous filter plate is used to divide the grid interface and The stripped chromatographic thin layer is positioned and eluted to the microporous plate; the microporous plate is used to locate and receive the components of the plane chromatogram, and prepare a microarray sample of the plane chromatogram component; the mass spectrometry analysis unit is used to dete...

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Abstract

The invention belongs to the field of active component screening, and relates to a sample processing device, a device for preparing a plane chromatography microarray and a biological activity screening system. The system contains planar chromatography, grid plate, microwell plate, biological activity detection unit and mass spectrometry analysis unit; the system is used to prepare planar chromatography into a "planar chromatography component microarray" sample, and use this sample to carry out biological activity tests, focus Mass spectrometry analysis was carried out on the array unit with significant biological activity; the dependence relationship between the intensity of biological activity and the quasi-molecular ion peak intensity of the biological mass spectrum of the corresponding array unit and the coupling degree of their chromatographic behavior were used as indications, from complex components with complex components and different contents Analysis of biologically active ingredients in ingredients. The system of the present invention does not need to separate and purify chemical components one by one in advance, does not need comprehensive analysis and detection, does not rely on molecular diversity compound libraries, samples can be amplified in parallel, has good compatibility, high throughput, and digitalization, greatly improving screening efficiency and reducing work quantity and cost.

Description

technical field [0001] The invention belongs to the technical field of drug analysis and biological testing, and specifically relates to a sample processing device and a system containing the device for high-throughput screening of biologically active components in complex components by means of a planar chromatographic component microarray. Background technique [0002] Natural products, traditional Chinese medicine and its compound, artificial synthesis and bioengineering products, such as various material components in terrestrial animals and plants, marine organisms and microorganisms, and isolated biological secondary metabolites are potential medicinal resource compound libraries. Most of them have the characteristics of complex components and great differences in content. Screening new drugs with significant biological activity has always been a problem that bothers us. [0003] High-throughput drug screening technology is developing rapidly as a means to quickly disc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 邓亦峰林振鹏
Owner GUANGDONG MEDICAL UNIV
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