Sample processing device and biological activity screening system including the same

A sample processing and biological activity technology, which is applied in the field of drug analysis and biological testing, can solve the problems of difficulty in implementation and difficulty in determining the chemical composition of biological activity

Active Publication Date: 2019-08-23
GUANGDONG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to make each chromatographic spot fall into the same microwell pool as much as possible, this method requires that the planar chromatographic spots correspond strictly to the microwell plate array, which is difficult to implement for thin-layer chromatograms with irregular spot shapes and positions; For the case where a chromatographic spot falls into multiple microporous pools and there are multiple co-existing components in the microporous pools, it is difficult to determine the chemical composition of the biological activity

Method used

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  • Sample processing device and biological activity screening system including the same
  • Sample processing device and biological activity screening system including the same
  • Sample processing device and biological activity screening system including the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0142] A sample processing device

[0143] In this embodiment, the grid plate corresponding to the array format of the 384-well square-mouth microplate is taken as an example to illustrate the structure of the grid plate, which can be adjusted according to the array format of the microplate during practical application.

[0144] Such as image 3 The grid plate shown has a plurality of channel-type spaces (that is, the inner space of each grid) defined by the grid frame, and the channel-type space has an open first end and a second end, wherein on the open plane of the first end, the area of ​​the grid frame defining each channel-shaped space is 10% of the area of ​​the first end of the channel-shaped space. At this time, the frame thickness of each grid frame at the first end is narrow enough to form a sharp edge, which can be used to cut the chromatographic thin layers of the planar chromatogram, ensuring that all the chromatographic thin layers can be cut and separated. Th...

Embodiment 2

[0147] Embodiment 2 A device for preparing planar chromatographic microarray

[0148] The device contains a plane chromatogram, a grid plate, a microporous filter plate, and a microporous plate corresponding to each other in an array format. And a biological activity detection unit and a mass spectrometry analysis unit.

[0149] The planar chromatography is used to construct the planar chromatographic fingerprint of the sample to be screened; the grid plate is used to divide and peel off the chromatographic thin layer of the planar chromatographic fingerprint according to the microplate array format; the microporous filter plate is used to divide the grid interface and The stripped chromatographic thin layer is positioned and eluted to the micro-orifice plate; the micro-orifice plate is used to position and receive the components of the plane chromatogram, and prepare a microarray sample of the plane chromatogram components.

[0150] In this embodiment, the chromatographic sp...

Embodiment 3

[0152] Embodiment 3 A kind of biological activity screening system

[0153] Such as Figure 13 The biological activity screening system shown includes a one-to-one array format of planar chromatography, grid plate, microporous filter plate, micropore plate, biological activity detection unit and mass spectrometry analysis unit.

[0154] The planar chromatography is used to construct the planar chromatographic fingerprint of the sample to be screened; the grid plate is used to divide and peel off the chromatographic thin layer of the planar chromatographic fingerprint according to the microplate array format; the microporous filter plate is used to divide the grid interface and The stripped chromatographic thin layer is positioned and eluted to the microporous plate; the microporous plate is used to locate and receive the components of the plane chromatogram, and prepare a microarray sample of the plane chromatogram component; the mass spectrometry analysis unit is used to dete...

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Abstract

The invention belongs to the field of biological activity screening, and relates to a sample processing device, a device for preparing a planar chromatography microarray and a biological activity screening system. The system includes a planar chromatography, a grid plate, a microplate, a biological activity detection unit and a mass spectrometry unit; the system is used for preparing the planar chromatography into a planar chromatographic component microarray sample, and the sample can be taken to carry out a biological activity test and focused on the significant biological activity detectionunit to carry out mass spectrometry; and the dependency relation between biological activity intensity and the biomass spectrometry excimer ion peak intensity of corresponding array units and chromatographic behavior coupling degrees of the two are taken as indications, so that biological activity components can be analyzed from complex components with complex components and intervallic content.The system does not need to perform separation and purification on chemical components one by one in advance and complete analysis and detection, does not depend on a molecular diversity compound library and is good in compatibility, high in flux and digitized, and samples can be amplified in parallel, so that screening efficiency can be greatly enhanced, and workloads and costs can be reduced.

Description

technical field [0001] The invention belongs to the technical field of drug analysis and biological testing, and specifically relates to a sample processing device and a system containing the device for high-throughput screening of biologically active components in complex components by means of a planar chromatographic component microarray. Background technique [0002] Natural products, traditional Chinese medicine and its compound, artificial synthesis and bioengineering products, such as various material components in terrestrial animals and plants, marine organisms and microorganisms, and isolated biological secondary metabolites are potential medicinal resource compound libraries. Most of them have the characteristics of complex components and great differences in content. Screening new drugs with significant biological activity has always been a problem that bothers us. [0003] High-throughput drug screening technology is developing rapidly as a means to quickly disc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 邓亦峰林振鹏
Owner GUANGDONG MEDICAL UNIV
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