Antiviral drugs
A virus and virus target technology, which is applied in the field of angiotensin II signal inhibitors, treatment or prevention of single-strand retrovirus infection of subjects, and can solve problems such as the difficulty of designing safe and effective antiviral drugs
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[0391] Example 1 - Antiviral Properties of Angiotensin II Signaling Inhibitors
[0392] Materials and methods
[0393] Cells: HeLa cells (ATCC CCL-2) were cultured in growth medium (supplemented with 10% (v / v) fetal calf serum (FCS), 10 mM HEPES, 2 mM L-glutamine, 100 U / mL penicillin and 100 μg / mL Streptomycin in EMEM). African green monkey renal epithelial Vero cells (ATCC CRL-81 ) were cultured in DMEM supplemented with 10% (v / v) FCS, 100 U / mL penicillin and 100 μg / mL streptomycin. All cells at 37°C and 5% CO 2 / 95% air atmosphere.
[0394] Viruses: All virology work was performed at the Australian Animal Health Laboratory, part of the Commonwealth Scientific and Industrial Research Organization (CSIRO). HeV (Hendra / Equus / 1994 / Hendra), NiV (Nipah / Malaysia / Human / 99) and RSV (strain A2) were passaged in Vero cells. Influenza A / WSN / 33 (H1N1) (a gift from Professor Lorna Brown, University of Melbourne) was passaged in the allantoic fluid of 10-day-old embryonated specific p...
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