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Method for detecting drug concentrations of avibactam and ceftazidime in blood plasma

A ceftazidime and plasma technology, applied in the field of drug analysis, can solve the problems of large sample volume, high cost, narrow linear range and the like of the detection method, and achieve the effects of simple and rapid detection method, accurate pretreatment process and high sensitivity

Inactive Publication Date: 2020-09-08
南京品生医学检验实验室有限公司
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  • Application Information

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Problems solved by technology

Literature (Liang Jiancheng, HPLC method for determination of ceftazidime blood concentration after micropump administration [J]. Chinese Pharmacist, 2008,11) The published detection method requires a large sample size, a narrow linear range, and only detects one drug. Not suitable for clinical development; the detection method disclo

Method used

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  • Method for detecting drug concentrations of avibactam and ceftazidime in blood plasma
  • Method for detecting drug concentrations of avibactam and ceftazidime in blood plasma
  • Method for detecting drug concentrations of avibactam and ceftazidime in blood plasma

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Embodiment 1

[0105] 1. Experimental materials and instruments

[0106] 1. Materials: The samples were collected from the outpatient clinic of Nanjing Drum Tower Hospital in May 2020.

[0107] 2. Instruments: Xevo TQ-S triple quadrupole mass spectrometer (Waters Corporation); UPLC I-Class ultra-high performance liquid chromatography system (with automatic sampler, Waters Corporation); SCILOGEX D2012 high-speed desktop centrifuge (USA); Ultrapure water instrument (ELGA LabWater, UK); multi-tube vortex mixer (Vortex genie2, US); adjustable pipette (Eppendorf 0.5-10 μL, 10-100 μL, 100-1000 μL); glassware, graduated cylinder, etc.

[0108] 3. Reagent consumables: MS grade methanol (Fisher, USA); HPLC grade methanol (Honeywell, USA); MS grade acetonitrile (Fisher, USA); HPLC grade acetonitrile (Honeywell, USA); MS grade formic acid (Fisher, USA); Chromatographic column model: ACQUITY UPLC BEH C18 (2.1×100 mm, 1.7 μm) (Waters Corporation).

[0109] 4. Standards: Standards and their correspondin...

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Abstract

The invention discloses a method for detecting the drug concentration of avibactam and ceftazidime in blood plasma, and belongs to the technical field of drug analysis. The method specifically comprises the following steps: taking pretreated blood plasma, separating a to-be-detected substance from a blood plasma matrix by utilizing ultra-high performance liquid chromatography, obtaining a mass-to-charge ratio of the to-be-detected substance to a corresponding isotope internal standard substance by virtue of mass spectrometry, and calculating the contents of avibactam and ceftazidime by utilizing an isotope internal standard quantification method according to an established calibration curve. The isotope internal standard substances are avibactam-13C5 and ceftazidime-d5. The method disclosed by the invention is high in sensitivity, strong in specificity, accurate and simple in pretreatment process; separation and detection of avibactam and ceftazidime drugs in blood plasma are completedwithin 5.0 min, the method can be used for quantitative analysis of avibactam and ceftazidime in blood plasma clinically, and the simple and rapid detection method is provided for concentration monitoring of avibactam and ceftazidime clinically.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, in particular to a method for detecting drug concentrations of avibactam and ceftazidime in blood plasma. Background technique [0002] Avibactam (AVT) is a non-β-lactam β-lactamase inhibitor, and studies have shown that it has no antibacterial activity in clinically relevant doses. However, avibactam protects β-lactam antibiotics from degradation by β-lactamases and thus maintains the antibacterial activity of β-lactam antibiotics. Therefore, it can be used in combination with β-lactam antibiotics for the treatment of bacterial infections. Ceftazidime (CTD) belongs to the third-generation cephalosporin antibiotics. Ceftazidime has the characteristics of broad antibacterial spectrum and enzyme resistance. It has strong antibacterial activity against Pseudomonas aeruginosa. It is widely used in the treatment of various moderate and severe infections. Drug of choice for the treatment of com...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72G01N2030/045G01N2030/065
Inventor 成晓亮李美娟
Owner 南京品生医学检验实验室有限公司
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