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Application of phloretin in preparation of pig, sheep or bovine semen cryopreservation agent

A cryopreservative and cryoprotectant technology, which is applied in the preservation, application of human or animal body, and medical preparations containing active ingredients, etc., can solve the problems of low conception rate, many abnormal sperm, low sperm recovery rate, etc. Achieve the effect of maintaining fertilization ability, easy to promote, and reliable effect

Active Publication Date: 2020-09-15
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after freezing and thawing, 40%-50% of the sperm are still inactive, which seriously reduces the utilization rate of the bulls.
However, pig sperm and sheep sperm are more sensitive to freezing stimulation. After thawing, the sperm recovery rate is low, there are many abnormal sperm, and the conception rate is lower than the normal conception rate. Therefore, the freezing of pig sperm and sheep sperm is still in the experimental stage.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The preparation method of pig semen cryoprotectant of the present invention comprises the steps:

[0023] Step 1, prepare the frozen base solution: accurately measure 1.1g of glucose, 1.48g of citric acid, 2.42g of Tris, 0.06g of penicillin sodium, and 0.1g of streptomycin sulfate, dissolve them in 100ml double distilled water, adjust the pH to 6.21, and adjust the osmotic pressure 286mosm / L, prepared as frozen base fluid;

[0024] Step 2, accurately measure 80ml of frozen base liquid, add 20ml of fresh egg yolk, 6 μmol of phloretin, and mix to obtain a kind of livestock semen cryopreservative (I liquid) of the present invention;

[0025] Get 95ml of step 2 livestock semen cryoprotectant I liquid and add 5ml of glycerin, filter and sterilize, and cool to room temperature to obtain another livestock semen cryopreservative (II liquid) of the present invention for preserving pig semen. Keep it in the refrigerator at 4°C for the following tests.

[0026] Control group 1: ...

Embodiment 2

[0049] The preparation method of goat semen cryopreservation agent of the present invention, comprises the steps:

[0050] Step 1, preparing frozen base liquid;

[0051] Step 2, accurately measure 85ml of frozen base liquid, add 15ml of fresh egg yolk and 4 μmol of phloretin, and mix evenly to prepare a cryopreservative for livestock semen (I liquid) of the present invention;

[0052] Get 97ml of this embodiment step 2 livestock semen cryopreservation agent I liquid and add 3ml glycerin to be mixed with 100ml mixed solution, filter and sterilize, cool to room temperature, promptly obtain another kind of livestock semen cryopreservation agent (II liquid) of the present invention It is used to preserve goat semen and put it in a refrigerator at 4°C for the following tests.

[0053] Control group 2:

[0054] The difference between the control group 2 and the embodiment 2 is that it does not contain phloretin, and the other ingredients are the same.

[0055] The inventor has ca...

Embodiment 3

[0077] The preparation method of bovine semen cryopreservation agent of the present invention, comprises the steps:

[0078] Step 1, preparing frozen base liquid, the method is the same as in Example 1;

[0079] Step 2: Accurately measure 80 ml of frozen base solution, add 20 ml of fresh egg yolk and 10 μmol of phloretin, and obtain a cryopreservative for livestock semen (I liquid) of the present invention.

[0080] Get 94ml of the livestock semen cryoprotectant (I liquid) that this embodiment obtains and add 6ml glycerin and mix, filter sterilize, cool to room temperature, promptly obtain another kind of livestock semen cryopreservation agent (II liquid) of the present invention for use in Save the bovine semen and put it in a refrigerator at 4°C for the following experiments.

[0081] Control group 3:

[0082] The difference between control group 3 and embodiment 3 is that it does not contain phloretin, and the rest of the ingredients are the same.

[0083] The contriver ...

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PUM

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Abstract

The invention discloses an application of phloretin in preparation of a pig, sheep or bovine semen cryopreservation agent. Meanwhile, the invention discloses a cryopreservation agent containing phloretin and a freezing base solution. According to the invention, phloretin is used for cryopreservation of semen of pigs, sheep and cattle for the first time; the application is reliable in effect, easyto popularize and suitable for artificial insemination of pig, sheep and cattle tubule frozen semen, long-term storage of the semen after ultralow-temperature freezing and semen deposition after long-distance transportation, the sperm motility rate, acrosome integrity rate and plasma membrane integrity rate of the frozen-thawed pig, sheep and cattle can be remarkably increased, and the fertilization capacity of sperms is kept.

Description

technical field [0001] The invention belongs to the technical field of ultra-low temperature cryopreservation of mammalian semen, and in particular relates to the application of phloretin as an antifreeze protective agent in cryopreservation of livestock semen. Background technique [0002] Sperm freezing technology is an important innovation in artificial insemination. It not only solves the problem of long-term preservation of male animal semen, but also facilitates the preservation of genetic resources. It is also convenient to apply and is not limited by time and space. , which plays an important role in establishing a global gene bank of excellent species. The wide application of sperm freezing technology is conducive to improving the utilization rate of excellent breeding males, reducing the number of breeding males, reducing economic costs, and improving the efficiency of animal husbandry, especially in the field of artificial insemination. Play an important role. Se...

Claims

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Application Information

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IPC IPC(8): A01N1/02A61K31/122A61P39/06
CPCA01N1/0221A01N1/0226A61K31/122A61P39/06
Inventor 李青旺刘定帮杨丽吴琳
Owner NORTHWEST A & F UNIV
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