A foot-and-mouth disease virus non-structural protein 3b dominant epitope deletion-marked strain and its preparation method and application

Abstract

The invention provides a foot-and-mouth disease virus non-structural protein 3B dominant epitope deletion-marked strain as well as a preparation method and application thereof, which belong to the technical field of biotechnology and biological products. Foot-and-mouth disease virus non-structural protein 3B dominant epitope deletion marker strain, the foot-and-mouth disease virus obtained by completely missing the dominant epitope on the basis of non-structural protein 3B; GPYAGP to GPAAP mutation, GPYAGP to GPAAP mutation in 3B2, and GPYEGP to GPYEAA mutation in 3B3. The application of the marked virus strain in preparing the foot-and-mouth disease marked vaccine for distinguishing infection and vaccine immunity can meet the purpose of immune prevention and differential diagnosis, and can be used for effective prevention, control and purification of FMD in my country.

Description

technical field [0001] The invention belongs to the technical field of biotechnology and biological products, and in particular relates to a foot-and-mouth disease virus nonstructural protein 3B dominant epitope deletion-marked strain and a preparation method and application thereof. Background technique [0002] Foot and mouth disease (Foot-and-Mouth Disease, FMD) is an acute, febrile, highly contact disease that is caused by Foot-and-Mouth Disease Virus (FMDV) and mainly domestic animals such as pigs, cattle, sheep and wild cloven-hoofed animals. Infectious disease, the disease spreads rapidly, has a high incidence rate, and is extremely harmful, causing great harm to the development of animal husbandry. [0003] Immunization with traditional inactivated vaccines is the most effective means to prevent and control FMD in my country, but conventional inactivated vaccines cannot distinguish between vaccine immunization and wild virus-infected animals, resulting in the inabili...

AI Technical Summary

Problems solved by technology

However, the absence of FMDV G-H loop dominant antigenic epitope will seriously affect the immunogenicity of the vaccine, the attenuated vaccine has the risk of virulence reversion, and the deletion of large gene fragments will affect the replication ability of the virus. Therefore, this area still needs to develop more High-quality FMD-labeled virus vaccine candidate strains provide effective technical support for the effective prevention, control and purification of FMD and the construction of a disease-free area in my country

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