Method and system for detecting Rh blood group antigen and application thereof

A rh blood type, blood type antibody technology, applied in the direction of measuring devices, biological testing, material inspection products, etc., can solve the problems of easy deformation of reagent gel, generation of air bubbles, time-consuming and labor-intensive, etc., to achieve clear and easy-to-read reaction results, and get rid of gel Dependence, to avoid the effect of manual operation

Pending Publication Date: 2021-07-09
JIANGYIN LIBO MEDICINE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows for accurate identification of different types of hemoglobin (the protein that carries oxygen) without requiring complicated laborious procedures like histopathology analysis. It uses special reagents called labeled primers with unique properties such as coloring them when exposed to light during testing. By measuring changes caused by these colored particles after exposure, researcher can identify which ones were tested more effectively than others. Overall this innovative technique simplifies the process while improving accuracy and reliability over existing methods used for identifying various biological samples.

Problems solved by technology

This patented technical problem addressed in this patents relates to improving the accuracy and efficiency of identifying specific types or groups of hematopoietic stem/progenitor B lymphocytes from donor individuals based upon their ability to form allograftable glycoproteins due to inherited differences in HLA genes. Current techniques involve measuring autoimmunity markers like antihemoglobin protein 1B1 , complement factor VIII, and platelet alpha subunit beta 2 through enzyme assays, but they all suffer from limitations related to factors like dilution issues and potential interference effects associated with previous tests.

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  • Method and system for detecting Rh blood group antigen and application thereof
  • Method and system for detecting Rh blood group antigen and application thereof
  • Method and system for detecting Rh blood group antigen and application thereof

Examples

Experimental program
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Embodiment 1

[0054] Embodiment 1: A kind of method for detecting Rh blood group antigen (Rh anti-D)

[0055] This embodiment provides a method for detecting Rh blood group antigen (see the principle of detection figure 1 , for possible test results see figure 2 ), the method comprises the steps of:

[0056] (1) Coating: Dilute the mouse anti-human IgM antibody to a concentration of 1 μg / mL with carbonate buffer solution of pH 9.6 to obtain a dilution; add the dilution to a U-shaped microplate at an amount of 100 μL per well Afterwards, coat at 4°C for 12 hours to obtain a coated microwell plate;

[0057] (2) The first plate washing step: Add pH 7.2 PBS buffer solution to the coated microwell plate at an amount of 250 μL per well for washing, repeat washing 5 times, and the microwell plate needs to be washed every time. Blot the remaining liquid in to obtain washed microwell plate A;

[0058] (3) Blocking: After adding 250 μL per hole to the washed microwell plate A, the carbonate buff...

Embodiment 2

[0062] Embodiment 2: A kind of method for detecting Rh blood group antigen (Rh anti-D)

[0063] This embodiment provides a method for detecting Rh blood group antigen (see the principle of detection figure 1 , for possible test results see figure 2 ), the method comprises the steps of:

[0064] (1) Coating: Dilute the mouse anti-human IgM antibody to a concentration of 0.5 μg / mL with carbonate buffer solution of pH 8.9 to obtain a dilution; add the dilution to U-shaped microwells in an amount of 120 μL per well After the plate, it was coated at 2°C for 8 hours to obtain a coated microwell plate;

[0065] (2) The first plate washing step: Add pH 6.5 PBS buffer solution to the coated microwell plate in an amount of 200 μL per well for washing. Blot the remaining liquid in to obtain washed microwell plate A;

[0066] (3) Blocking: After adding the carbonate buffer of pH 8.9 containing BSA with a volume concentration of 0.1% and Tween20 with a volume concentration of 0.02% in...

Embodiment 3

[0070] Embodiment 3: A kind of method for detecting Rh blood group antigen (Rh anti-D)

[0071] This embodiment provides a method for detecting Rh blood group antigen (see the principle of detection figure 1 , for possible test results see figure 2 ), the method comprises the steps of:

[0072] (1) Coating: Dilute the mouse anti-human IgM antibody to a concentration of 10 μg / mL with carbonate buffer solution of pH 9.8 to obtain a dilution; add the dilution to a U-shaped microplate at an amount of 100 μL per well Afterwards, coat at 8°C for 16 hours to obtain a coated microwell plate;

[0073] (2) The first plate washing step: Add pH 7.8 PBS buffer solution to the coated microwell plate in an amount of 300 μL per well for washing, and repeat the washing 5 times. Blot the remaining liquid in to obtain washed microwell plate A;

[0074] (3) Blocking: After adding 300 μL per hole to the washed microwell plate A, the carbonate buffer containing pH 9.8 of BSA with a volume conc...

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Abstract

The invention relates to a method and a system for detecting an Rh blood group antigen and application thereof, and belongs to the technical field of biological detection. The invention provides a positive typing method for detecting an Rh blood group antigen based on solid phase adsorption, which comprises the following steps: adding red blood cells to be typed and an Rh blood group antibody into a container coated with second antibodies of other species, performing centrifuging, and observing whether the red blood cells to be typed are adsorbed at the bottom of the container coated with the second antibodies of other species; if the red blood cells to be typed are adsorbed at the bottom of the container coated with the second antibodies of other species, determining that the red blood cells to be typed and Rh blood group antigens corresponding to the Rh blood group antibodies are positive, and if the red blood cells to be typed are not adsorbed at the bottom of the container coated with the second antibodies of other species, determining that the red blood cells to be typed and Rh blood group antigens corresponding to the Rh blood group antibodies are negative. The method is high in sensitivity, strong in specificity and low in detection cost, has the advantage of easiness in automatic operation, and can avoid personal errors in the operation process.

Description

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Claims

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Application Information

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Owner JIANGYIN LIBO MEDICINE BIOTECH
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