71 results about "Solid phase adsorption" patented technology

Belonging to the technical field of soil and sediment pollution detection, the invention relates to a dispersive solid-phase extraction gas chromatography method for polychlorinated biphenyls (PCBs) in soil and sediment. The method includes: adding n-hexane into a soil or sediment sample to conduct ultrasonic extraction, when the extraction liquid is basically colorless and transparent, subjecting the extraction liquid to rotary evaporation till dry, adding n-hexane, then adding 50-200mg of a N-propylethylenediamine solid phase adsorption agent to carry out dispersive solid-phase extraction, performing centrifugal layering, and introducing 1microliter of the supernatant into a gas chromatography-electron capture detector to conduct detection, otherwise adding a concentrated sulfuric acid purification step after ultrasonic extraction. The detection limit of 7 polychlorinated biphenyls is 0.02-0.03 microgram/kg, the recovery rate is 72-115%, and the relative standard deviation (n=5) is 3.5-6.9%. The method provided by the invention is simple and sensitive, and can quickly realize batch detection of PCBs in soil and sediment.

A process for using solid-phase carrier to separate nucleic acid from biologic particles features that the nm-class magnetic microspheres emulsion or the specially treated fibrous membrane is used as solid-phase adsorbing medium to separate cell from biologic particle, the cell-cracking liquid is used to crack cell structure, the solid-phase adsorption and desorption are used to directly collect the biologic particles rich in nucleic acid from biologic specimen such as blood plasma, etc., and the nucleic acid is extracted. Its advantages include simple process, high speed, and easy autoamtization and miniaturization.

The invention discloses a device and a method for cooperatively removing gaseous pollutants by plasma-supergravity coupling. According to the device and the method disclosed by the invention, multiple gaseous pollutants are decomposed and oxidized by using high-energy electrons and active groups generated by low-temperature plasma discharge; in a process that the gaseous pollutants flow through a solid adsorbent reactor rotating at high speed, gas containing the pollutants are in sufficient contact with a solid adsorbent by means of a supergravity effect generated by high-speed rotation of gas and solid, to generate a gas phase-solid phase adsorption and chemical reaction to finish substance conversion and purification processes between multiple gaseous pollutants and the solid adsorbent, and thus the pollutants are converted into non-toxic and pollution-free substances. The device and the device are applicable to dry-process combined removal of multiple pollutants in flue gases and various wastes gas generated by combustion, gasification, incineration and the like in the fields of energy resources, chemical engineering, building materials, metallurgy and the like containing multiple gaseous pollutants.

The invention discloses a solid-phase adsorption material and an application thereof to a QuEChERS method. The solid-phase adsorption material disclosed by the invention adopts a carbon material, a modified silica gel material and a magnetic ferroferric oxide composite material as adsorption materials; and the price is low and the commercialization is easy to realize. The QuEChERS method comprises the following steps of: firstly extracting sample homogenate added with salt by using acetonitrile; then adding anhydrous magnesium sulfate to remove water and using the magnetic adsorption material to remove a large amount of interference objects in the acetonitrile; and finally, directly carrying out detection and analysis on a treated solution. The magnetic adsorption material has a magnetic separation performance and can be rapidly separated from the solution under the effect of an external magnetic field; and the operation is simple and the analyzing time is effectively reduced.

The invention discloses a microwave-assisted Soxhlet and solid-phase extraction coupling device, a microwave-assisted Soxhlet and solid-phase extraction coupling method and application of microwave-assisted Soxhlet and solid-phase extraction coupling. The microwave-assisted Soxhlet and solid-phase extraction coupling device comprises a microwave-assisted extractor, a Soxhlet extractor, solid-phase adsorbents, an extraction bottle, a reflux condensing pipe and the like, and extraction and purification are synchronously completed by means of coupling microwave-assisted Soxhlet extraction and solid-phase extraction. When in extraction, the solid-phase adsorbents are filled into a Soxhlet extraction device, the extraction bottle is directly placed in the microwave-assisted extractor for extraction after extraction solvents are added into the extraction bottle, analytes are collected into the extraction solvents, and interfering components in samples are adsorbed by the solid-phase adsorbents. High efficiency of microwave-assisted extraction, universality of Soxhlet extraction and high selectivity of solid-phase extraction are combined, the device is simple and convenient in operation, is conveniently used with an analysis instrument, and is suitable for extraction and purification of trace organics in complicated samples.

The invention relates to the technical field of marine sediment contamination detection, in particular to a dispersive solid-phase extraction-gas chromatography-mass spectrometry detection method of aphthalic acid ester environmental hormone in a marine sediment. The method comprises the following steps of: (1) sample collection, (2) sampling and ultrasonic extraction, (3) concentration, (4) dispersive solid-phase extraction purification, (5) concentration and constant volume, (6) detection by a gas chromatography-mass spectrometry detector, (7) standard curve drawing and (8) determination ofa recovery rate. The method is simple to operate; the ultrasonic extraction efficiency is high; pretreatment of a sample can be accomplished quickly; graphitized carbon black is used as a solid-phaseadsorbent for the first time; the dispersive solid-phase extraction efficiency is high; a purification effect is good; the method is higher in sensitivity, recovery rate and repeatability; a detection limit of 16 phthalic acid esters is 0.1-0.25 micrograms per kg; the recovery rate is 78-117%; a relative standard deviation (n=5) is 2.4-6.8%; and the method can be applied to the accurate determination of a phthalic acid ester content in the marine sediment.

The invention discloses a detection method for odor substances in exhaust gas of the perfume and essence industry. According to the invention, exhaust gas discharged by the perfume and essence industry is directly or indirectly acquired, then solid-phase micro-extraction (SPME) or solid-phase adsorption-thermal desorption technology are employed for extraction of a sample; and extraction temperature and extraction time are adjusted and GC-MC is cooperatively used for analysis of the exhaust gas sample. The detection method is high in efficiency, simple to operate and applicable to analysis of odor substances with any concentration. An airbag method and negative pressure sampling technology are cooperatively used, an appropriate sampling point is selected and a representative gas sample is acquired, so original information of the sample is maintained to a greatest extent. A sampling bag has good airtightness, and a sample in the bag can be maintained for more than one month. SPME pretreatment technology is employed, so the advantages of greenness, rapidness, no pollution and repeated usability can be obtained. The detection method is applicable to both exhaust gas discharged in the perfume and essence industry and to detection of exhaust gas of industries like petroleum, the chemical industry, food, medicine and cosmetics.

The invention relates to a method for conducting multiplex quantitative detection on miRNA. The method comprises the steps of reverse transcription, single-chain complementary extension, S1 nuclease digestion, solid phase adsorption extracting and fluorescent quantitative detection. According to the method for conducting the multiplex quantitative detection on the miRNA, on the basis of fluorescent quantitative PCR, the single-chain digestion function of S1 nuclease and the nucleic acid solid phase adsorption extraction technology are applied in a combined mode, and a breakthrough in the flux of miRNA quantitative detection is achieved.

The invention discloses a method for removing aluminum from rare earth material solution by organic acid complexation and solid-phase adsorption, which includes such steps as chelating reaction between citrate radical and aluminum ions in the rare earth material solution, adsorbing aluminum citrate by utilizing porous characteristics of porous polyethylene styrene resin and activated carbon, fixing aluminum ions on the surface of an adsorbent, and removing aluminum from the rare earth material solution. The removal rate of aluminum ions is 80% or above and the loss rate of rare earth is no more than 5%. Compared with other existing technologies, the existing method has low requirements on equipment, is easy to operate, and avoids the problem that aluminum hydroxide flocculent precipitationis difficult to filter, and the existing adsorbent material can be recycled, thus reducing the production cost. The invention provides an extremely effective, economical and practical way for removing aluminum from the rare earth material solution.

The invention discloses a method for separating and recycling acid and metal ions in steel pickling waste liquid. The method comprises the following steps of: (1) loading a sample: loading the steel pickling waste liquid containing ferrous ions into a chromatographic separation column containing a solid-phase adsorption material, and collecting effluent in three sections, namely low-concentrationrecovery area effluent, ferrous ion recovery area effluent and original-concentration recovery area effluent; (2) eluting: eluting by using eluent, and collecting eluent in three sections, namely original concentrated recovery area eluent, acid recovery area eluent and low-concentration recovery area eluent; and (3) repeating the step (1) and the step (2), and stopping circulation until a solid-phase separation material has no separation effect on acid and ferrous ions. Compared with the prior art, the method is reasonable and controllable in process, low in equipment requirement and high in separation efficiency; the acid recovery rate of the method is higher than 95%; the metal ion recovery rate of the method is higher than 98; the acid recovery concentration ct/c0 is greater than or equal to 80%; and the metal salt recovery concentration ct/c0 is greater than or equal to 90%.

A flow control assembly for use in preparation of samples for analysis by solid phase extraction includes a housing assembly having a first passage communicating with a needle bore and locating a solid phase sorbent, a second fluid port, and a valve for selective aspiration or delivery of fluid across a solid phase sorbent, or bypassing the solid phase sorbent. Independent flow paths are provided for a sample via the needle, and of the elution solvent via a separate port. The valve includes relatively slideable first and second members, the second member including an elongate shank that is relatively reciprocably slideable for effecting selective aspiration or delivery of fluid between first and second relative positions. A third fluid port may be provided communicating with the elongate second passage for selective fluid communication with a port in a shank when the shank is at the second relative position.

The invention relates to the field of a solid phase extraction-gas chromatography-mass spectrometry combined technology and provides a method for detecting camellia nitidissima chi oil type volatile ingredients. The method comprises the following steps: detecting the camellia nitidissima chi oil type volatile ingredients by a solid phase extraction adsorption-gas chromatography-mass spectrometry combined process, wherein the solid phase extraction is headspace stir bar solid phase adsorption extraction, and a stir bar is coated with a two-dimensional or three-dimensional graphene-polymer coating; the materials of the graphene-polymer coating include graphene and graphene modified materials, and the graphene modified materials include graphene oxide, graphene-polyethylene glycol dimethacrylate, graphene-polypyrrole, graphene-polymethyltrimethoxy silane and a graphene nanomaterial. The method has the characteristics of accurate detection, high sensitivity, good selective adsorption effect and long service life.

The invention discloses a needle extraction device for enriching volatile benzene series and a using method of the needle extraction device. The device is provided with a stainless steel needle head,an expansion needle cylinder, a reinforcing air guide device and an air exhaust device or an air injection device, wherein the stainless steel needle head is provided with a needle base; the expansionneedle cylinder is detachably connected with the needle base in an airtight mode; the reinforcing air guide device is connected with the tail part of the expansion needle cylinder through a sealing connection base, and the air exhaust device or the air injection device is detachably connected with the tail part of the reinforcing air guide device in a sealed mode; the expansion needle cylinder isfilled with a solid-phase adsorbent, a heat conduction device is arranged on the outer wall of the needle cylinder, the heat conduction device is connected with a temperature controller, and the expansion needle cylinder is made of a heat conduction metal material. The device has the advantages of short enrichment time, simple operation and high specificity selectivity, the defects of poor permeability, low reproducibility, difficult preparation and the like in an existing needle extraction technology can be overcome, and the device can be combined with gas chromatography in use, is convenient to use and provides a novel extraction technology for accurate analysis and detection of trace gas.

A solid-liquid apparatus with cylindrical shape for cleaning the foul gas is a cylindrical main body divided into upper solid-phase adsorption chamber and lower liquid-phase one by a microporous gas distributing plate and with a foul gas inlet tube in said liquid-phase chamber, a clean gas outlet tube on the top, solid filler inlet and outlet, and liquid inlet and outlet.

The invention proposes a method for efficiently polymerizing proteins, which can greatly reduce the proportion of unpolymerized proteins in a mixture of polymerized proteins, without the need for subsequent steps of removing unpolymerized proteins, and has short polymerization reaction time and simple polymerization process. The method comprises the following steps: firstly mixing a solid-phase adsorption medium with protein, and the solid-phase adsorption medium is anion exchange resin or cation exchange resin; after the protein is adsorbed on the solid-phase adsorption medium, washing to remove Adsorb the protein on the medium; then add polyaldehyde molecules to polymerize the protein; after the polymerization is completed, add a reducing agent to reduce the double bond of Schiff's base to a stable single bond, wash to remove other reactants, and obtain a polymerized protein.

The invention relates to a method for detecting the residue amount of fenvalerate pesticide in tea. The method comprises the first step of preparing a sample, wherein tea is dried, ground and screened; the second step of rough extracting of fenvalerate, wherein tea powder is put into a centrifuge tube, water is added for wetting, petroleum ether and mixture interior label working solution are added, vibrating for mixing is conduced, anhydrous magnesium sulfate, sodium chloride, sodium citrate, sodium hydrogen citrate and toluene are added, uniform vibrating is conducted, and centrifugation is conducted; the third step of purifying rough extract of fenvalerate, wherein supernate is placed into the centrifuge tube, anhydrous magnesium sulfate and N-propyl ethylenediamine bonding solid-phase adsorbent are added, uniform vibrating is conducted, and centrifugation is conducted; the fourth step of extracting fenvalerate, wherein supernatant is taken, toluene-isooctane mixed solution is used for extraction, concentration and filtration on a Florisil column; the fifth step of detection of chromatography. The extracting method is simple, the effect is good, and result integrals are processed more easily, conveniently and accurately. According to the method, the average recovery rate ranges from 93.21% to 103.75%, the relative standard deviation ranges from 3.28% to 6.20%, requirements are met, and accuracy and reliability are achieved.

The invention relates to an extraction process of salidroside. The extraction process comprises: 1, carrying out mixed incubation on a rhodiola rosea extract and magnetic particles to obtain a mixed solution a; 2, attracting and separating the mixed solution a through a magnet or in a magnetic field, and obtaining adsorption magnetic particles b; 3, soaking the adsorption magnetic particles b in an eluent A and then attracting and separating through a magnet or in a magnetic field, and obtaining fishing liquid c and elution magnetic particles d; 4, acetylating the fishing liquid c, mixing the acetylated fishing liquid c with penta-acetyl glucose, adding the eluted magnetic particles d, and carrying out a reaction to generate a reactant e; and 5, carrying out deacetylation treatment on the reactant e to obtain salidroside, wherein the magnetic particles are prepared by immobilizing a nickel or palladium complex on the surfaces of magnetic silicon dioxide material particles by tyramine. The magnetic materials are used for carrying out solid-phase adsorption on the extract, so that the extraction rate is high. Meanwhile, in the subsequent process, a magnetic material is used for catalyzing non-target substances to be converted into salidroside, so that the purity of salidroside is improved.

The invention relates to a dynamic superheated liquid water extraction device and a method for veterinany drug residues in food. The device comprises a liquid delivery pump, a temperature control cabinet, an extraction pond and an adsorption pipe, wherein a stainless steel preheating coil and the extraction pond are arranged in the temperature control cabinet. The adsorption pipe, the extraction pond and the liquid delivery pump are connected through a switching valve and are switched according to an operation requirement. According to the method, the superheated liquid water extraction is combined with a C18 padding solid phase adsorption technology, so that the extraction efficiency is increased, the operation steps are simplified and different sizes of the extraction pond and padding elution modes can be selected according to the difference in food classification. The method is a new technology for extracting the veterinany drug residues in food, with high efficiency, low consumption, environmental protection and excellent application prospect.

The invention belongs to the technical field of radioactive waste water treatment, and relates to a solid-phase adsorbing material, a preparation method thereof and an application of the adsorbing material. The solid-phase adsorbing material comprises extraction agents with structures as shown in a formula (a) or (b). The solid-phase adsorbing material can achieve good adsorption performances for+3, +4 and +6 valence state (+6 valence state ions are actinium acyl ions) actinide ions and lanthanide ions within a wide acidity range.

The invention relates to a method for screening sulfonamide residues in goat milk. The method comprises the following steps: pretreating goat milk with a mixed aqueous solution of acetic acid and acetonitrile, anhydrous magnesium sulfate and anhydrous sodium acetate; adjusting the pH value of the obtained supernatant to 2-6, adding a dispersed solid-phase adsorbent prepared from porous graphene and multi-walled carbon nanotubes, carrying out vortex and centrifugation, performing separating to obtain the supernatant, adding an eluent, uniformly performing mixing, performing centrifuging, evaporating the supernatant to dryness, and performing redissolving with an ammonium formate aqueous solution to obtain a to-be-detected solution; using blank goat milk to obtain to-be-detected solution containing the sulfanilamide medicine standard substance, and analyzing the two solutions to obtain quantitative ion chromatographic peaks, the corresponding retention time, a molecular ion mass-to-charge ratio and a fragment ion mass-to-charge ratio; and finally, comparing the information to obtain the peak area of the quantitative ion chromatographic peak of each sulfonamide drug, and calculating the concentration of each sulfonamide drug in the goat milk in combination with a matrix matching mixed standard working curve.

The invention discloses a pretreatment device for pesticide residue detection. A water-soluble solvent extraction device B comprises a device base, a solution box, an extraction device, a distiller, a condenser and a collection box; a non-water-soluble solvent redistribution device A comprises a base, a mixing bottle, an upper supporting seat, a side stand column, a filter bottle, a drainage tube, a filter screen, a drainage pump and a mixing bottle oscillation mechanism; and a solid-phase adsorption column purification device C comprises a column body with a cylindrical shell structure and a column cap positioned at the upper end of the column body. The water-soluble solvent extraction device B is simple in structure and good in using effect, water-soluble substances are rapidly extracted, the extraction speed and the extraction rate are increased, and the overall practicability is improved. The non-water-soluble solvent redistribution device A is used for vibrating a non-water-soluble solvent in the mixing bottle so as to separate a non-water-soluble substance and the structure is simple, and use and operation are convenient. The solid-phase adsorption column purification device C is simple in structure and convenient to operate and control and the column body can be vertically supported on an operation table top through a support mechanism.

The invention provides a sealed device for testing the yield of secondary organic aerosol and a use method of the sealed device. The sealed device comprises a polytetrafluoroethylene environment chamber, wherein the volume of the polytetrafluoroethylene environment chamber is 1m<3>; an ultraviolet lamp and a fan made of a polytetrafluoroethylene material are respectively arranged at two ends of the environment chamber; a sample feeding hole and a sampling hole are formed in the wall of the environment chamber; the sample feeding hole is connected with a spherical glass bulb of which the volume is 30cm<3>; organic matters are blown and volatilized in the glass bulb in a nitrogen blowing mode and are further fed into the environment chamber; subsequently, the fan is powered on; after the organic matters are distributed in balance, the ultraviolet lamp is powered on to excite the organic matters to generate the secondary organic aerosol; the generated secondary organic aerosol is collected by using a Tena*TA solid-phase adsorption tube, is desorbed by using a thermal desorption device, and is fed into a gas chromatograph-mass spectrometer for testing. By adopting the sealed device and the use method, multiple uncertain factors of conventional study experiment can be excluded, pollution to the ambient environment can be avoided, the aerosol distribution uniformity can be maintained, and the experimental environment is not interfered.

The invention provides a magnetic PAMAM dendrimer material modified by p-mercaptobenzoic acid. The preparation method of the material comprises the following steps: preparing amino-modified magnetic nanoparticles, carrying out n-generation PAMAM grafting on the amino-modified magnetic nanoparticles, and modifying the grafted product with p-mercaptobenzoic acid to obtain the p-mercaptobenzoic acid-modified magnetic PAMAM dendritic polymer material. The 4-MBA modified magnetic PAMAM dendritic polymer material is obtained by modifying a magnetic PAMAM dendritic polymer with p-mercaptobenzoic acid, and the material can realize simple, rapid, sensitive and effective determination of polycyclic aromatic hydrocarbons (PAHs) in an environmental water sample. The material used as a solid phase adsorbent has better linearity within the range of 0.1-300mu g/L, and the detection limit range is 0.014-0.055 mu g/L. Compared with other conventional methods, the method has the advantages of simplicity, durability, easiness in separation and high enrichment efficiency.

The invention relates to a method and a system for detecting an Rh blood group antigen and application thereof, and belongs to the technical field of biological detection. The invention provides a positive typing method for detecting an Rh blood group antigen based on solid phase adsorption, which comprises the following steps: adding red blood cells to be typed and an Rh blood group antibody into a container coated with second antibodies of other species, performing centrifuging, and observing whether the red blood cells to be typed are adsorbed at the bottom of the container coated with the second antibodies of other species; if the red blood cells to be typed are adsorbed at the bottom of the container coated with the second antibodies of other species, determining that the red blood cells to be typed and Rh blood group antigens corresponding to the Rh blood group antibodies are positive, and if the red blood cells to be typed are not adsorbed at the bottom of the container coated with the second antibodies of other species, determining that the red blood cells to be typed and Rh blood group antigens corresponding to the Rh blood group antibodies are negative. The method is high in sensitivity, strong in specificity and low in detection cost, has the advantage of easiness in automatic operation, and can avoid personal errors in the operation process.