The invention discloses a method for separating two
bibenzyl compounds from ordinary oil tea camellia. The method comprises the following steps: (1) raw
materials preparation, namely cleaning ordinary oil tea camellia, removing impurities, airing and crushing; (2) extraction, namely heating and extracting the ordinary oil tea camellia with an
ethanol solution, filtering and concentrating to obtain concentrated liquid; (3) separation and purification, namely
processing the concentrated liquid with
macroporous resin, sequentially eluting with
distilled water and the
ethanol solution, respectively collecting, and merging eluants, so as to obtain an eluant mixture; (4)
silica gel separation, namely concentrating the eluant mixture, carrying out
silica gel chromatography, eluting with a mixed liquid of
dichloromethane, methyl
alcohol and water, and respectively collecting a target distillate fractions; and (5)
liquid phase preparation, namely respectively concentrating the target distillate fractions, dissolving, and carrying out semi-preparation and collection with the
liquid phase. Two target product components are confirmed by identification; the yields can be up to 37mg / kg and 200mg / kg; the purity is greater than 90%; and the method disclosed by the invention is simple and convenient in preparation process, and suitable for industrial production.