Mouse submandibular venous blood collecting method

Abstract

The invention relates to a mouse submandibular venous blood collecting method, and belongs to the technical field of blood collection. The mouse submandibular venous blood collecting method comprises the following steps that 1, after a mouse is fixed, the blood collecting location is disinfected, wherein the blood collection location is the submandibular venous plexus blood vessel of the masseter edge at the back of the mandible of the mouse head; 2, lancet is vertically inserted into the blood collecting location and rotates clockwise by 90 degrees, the lancet is pulled out, and blood is collected. The blood collecting method is rapid, simple and small in trauma, blood collection can be conducted on the same blood collection location repeatedly, the blood collection capacity of each time is controllable, animals recover rapidly after blood collection and conform to the 3R principles, and very good application and popularization value is achieved.

Description

technical field [0001] The invention relates to the technical field of blood collection, in particular to a method for blood collection from the submandibular vein of mice. Background technique [0002] In animal experimental research, blood samples of experimental animals are often collected for physiological, biochemical, and microbiological, parasite, and other detection and analysis. At present, the commonly used blood collection methods for rats and mice include orbital blood collection, enucleation blood collection, cardiac blood collection and tail clipping blood collection. However, each of the original blood collection methods has its own advantages and disadvantages. The orbital blood collection method: the blood collection volume is about 0.2 to 0.3 mL. Although blood collection can be repeated, it is easy to damage the eyeball and cause infection. The blood collected is easy to coagulate and may be small. The mouse is "unrecognizable"; the blood collection metho...

AI Technical Summary

Problems solved by technology

However, among the many original blood collection methods, each has its own advantages and disadvantages. The orbital blood collection method: the blood collection volume is about 0.2-0.3mL. Although the blood collection can be repeated, it is easy to damage the eyeball and cause infection. The blood collected is easy to coagulate and may be small. Rats are "unrecognizable"; eyeball enucleation and blood collection method: the blood collection volume is slightly larger, about 0.3-0.4mL, but the blood collection speed cannot be controlled, and the blood is easy to soak into the hair around the eyes to cause pollution, and blood collection cannot be repeated, and it can also cause blindness in mice , infection, and poor hemostasis can cause death of mice, and it does not conform to the basic principles of animal welfare; heart blood collection method: blood collection volume can reach 0.5-0.6mL, but due to the small size of mice, small hearts, and fast heart beats, It is difficult to fix the position during blood collection, so the operation is difficult, requires anesthesia, and is likely to cause animal death due to operational problems, so it is more suitable for rabbit blood collection; tail-cut blood collection method: although blood can be collected multiple times in a row, the amount of blood collected each time is extremely high. Small, less than 0.1mL, and the blood collection speed is slow, not suitable for mouse experiments with large samples and more blood volume

The prior art lacks a mouse blood collection method that can be promoted and applied on a large scale

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