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Method for showing fingerprints through silanization nanometer magnetic powder

A nano-magnetic powder and silanization technology, which is applied in DNA preparation, recombinant DNA technology, human identification, etc., can solve the problems of difficult DNA extraction, and achieve the effects of reducing experience dependence, high efficiency, and good stability

Active Publication Date: 2014-03-12
深圳柏悦基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem solved by the present invention is to overcome the defects and deficiencies of the prior art, and to solve the conflict between the existing fingerprint imaging technology and fingerprint DNA extraction technology. The characteristics of all magnetic powders in the technology can reduce its damage to the DNA in the fingerprint shedding cells, thereby solving the problem of difficult DNA extraction after fingerprint imaging, and have developed an efficient, simple, and cheap method that can simultaneously use nano-magnetic beads as materials for Fingerprints are developed and their DNA extracted simultaneously

Method used

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  • Method for showing fingerprints through silanization nanometer magnetic powder
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  • Method for showing fingerprints through silanization nanometer magnetic powder

Examples

Experimental program
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Effect test

Embodiment 1

[0032] The specific implementation steps are as follows:

[0033] 1) Simulate fingerprints, press the aluminum alloy 4 times with fingers, use non-silanized micron magnetic powder for sample 1, use silanized micron magnetic powder for sample 23, use non-silanized nano-magnetic powder for sample 3, and use nano-magnetic powder of the present invention for sample 4. The silanized nano-magnetic powder used in the invention is a product listed by Wuhan Wawa Thiana Technology Development Co., Ltd., and the nano-magnetic powder is gently brushed on the surface of the detection area with a magnetic powder brush, and the fingerprint can be seen;

[0034] 2) Use a cotton swab to wipe the aluminum alloy surface where the original fingerprint is located, transfer the fingerprint in two steps of dry and wet, and then put the cotton swab into the centrifuge tube respectively;

[0035] 3) Add 350 μl lysate and 20 μl proteinase K to the centrifuge tube, and incubate at 56 degrees for 3 hours...

Embodiment 2

[0049] The specific implementation steps are as follows:

[0050] 1) To simulate a fingerprint, the finger presses the plastic steel material 4 times, sample 1 uses non-silanized micron magnetic powder, sample 2 uses silanized micron magnetic powder, sample 3 uses non-silanized nano-magnetic powder, and sample 4 uses the nano-magnetic powder of the present invention. The silanized nano-magnetic powder used in the invention is a product listed by Wuhan Wawa Thiana Technology Development Co., Ltd., and the nano-magnetic powder is gently brushed on the surface of the detection area with a magnetic powder brush, and the fingerprint can be seen;

[0051] 2) Use a cotton swab to wipe the plastic steel material where the original fingerprint is located, transfer the fingerprint in two steps of dry and wet, and then put the cotton swab into the centrifuge tube respectively;

[0052] 3) Add 350 μl lysate and 20 μl proteinase K to the centrifuge tube, and incubate at 56 degrees for 3 ho...

Embodiment 3

[0066] The specific implementation steps are as follows:

[0067] 1) To simulate a fingerprint, press the plastic steel material 4 times with your fingers, and place it for a week. Sample 1 uses non-silanized micron magnetic powder, sample 2 uses silanized micron magnetic powder, sample 3 uses non-silanized nano-magnetic powder, and sample 4 uses the nano-magnetic powder of the present invention. , the silanized nano-magnetic powder used in the present invention is a listed product of Wuhan Wawa Thiana Technology Development Co., Ltd. After one week, the nano-magnetic powder is gently brushed on the surface of the detection area with a magnetic brush, and the fingerprint can be seen;

[0068] 2) Use a cotton swab to wipe the plastic steel material where the original fingerprint is located, transfer the fingerprint in two steps of dry and wet, and then put the cotton swab into the centrifuge tube respectively;

[0069] 3) Add 350 μl lysate and 20 μl proteinase K to the centrifu...

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Abstract

The invention relates to a method for showing fingerprints through silanization nanometer magnetic beads. Traditional magnetic powder is replaced by silanization nanometer magnetic powder, the fingerprints are shown through a magnetic powder brush development method, then cast-off cells of the fingerprints can still be used for DNA extraction after fingerprint showing, the whole process is sensitively, conveniently and quickly carried out, the fingerprints can be clearly shown, and genomic DNAs extracted after fingerprint showing are high in purity and stable and reliable in quality. The method can be applied to conventional fingerprint showing in reconnoitering and case solving and can also be applied to cases which can not be recognized by people because of the common phenomena that fingerprints are incomplete, not neat and obscure and characteristics of the fingerprints can not be shown clearly in reconnoitering and case solving.

Description

technical field [0001] The invention relates to a method for comprehensively using silanized nano-magnetic beads to display fingerprints. In the present invention, silanized nano-magnetic powders are used to replace traditional magnetic powders, and fingerprints are displayed by magnetic powder brush development. After the fingerprints are developed, the exfoliated cells of the fingerprints can still be used for DNA extraction. The process is sensitive and convenient, the fingerprint development is clear, and the genomic DNA extracted after fingerprint development has high purity and stable and reliable quality. It can be used for conventional fingerprint development in the detection of crimes, and it can also be used in cases where it is difficult to identify individuals due to the common phenomena of incomplete, blurred, and indistinct features in the detection of crimes. Background technique [0002] As a high-tech technology in the 21st century, nanotechnology has become...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B5/117C12N15/10
Inventor 聂棱李红闪黄小浩刘书童
Owner 深圳柏悦基因科技有限公司
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