Method for screening sulfonamide residues in goat milk

A technology of sulfa drugs and goat milk, which is applied in the field of screening sulfa drug residues in goat milk, can solve the problems of time-consuming operation and high cost, and achieve the effect of eliminating the influence of interfering substances and high adsorption capacity

Active Publication Date: 2021-07-23
SHAANXI UNIV OF SCI & TECH
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Problems solved by technology

[0004] In view of the complexity of animal-derived food matrices, the QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method is widely used in the detect

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  • Method for screening sulfonamide residues in goat milk
  • Method for screening sulfonamide residues in goat milk
  • Method for screening sulfonamide residues in goat milk

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[0040] The principles and advantages of the present invention will be explained and described below through specific embodiments, so that those skilled in the art can better understand the present invention. The following description is only exemplary and does not limit its content.

[0041] A method for screening sulfonamide drug residues in goat milk of the present invention adopts the method of dispersive solid phase extraction-ultra-high performance liquid chromatography quadrupole electrostatic field orbital ion trap mass spectrometry, comprising the following steps:

[0042] 1) Preparation and characterization of dispersed solid phase adsorbent

[0043] Preparation of porous graphene (porous graphene, PG): Add 0.8-1.2g of 2000-3000 mesh graphite powder into 20-25mL of concentrated sulfuric acid, cool down to 0-4°C in an ice-water bath, stir with a magnetic stirrer, and stir The speed is 20-30rpm, which is good for H 2 SO 4 In full contact with graphite, while slowly a...

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PUM

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Abstract

The invention relates to a method for screening sulfonamide residues in goat milk. The method comprises the following steps: pretreating goat milk with a mixed aqueous solution of acetic acid and acetonitrile, anhydrous magnesium sulfate and anhydrous sodium acetate; adjusting the pH value of the obtained supernatant to 2-6, adding a dispersed solid-phase adsorbent prepared from porous graphene and multi-walled carbon nanotubes, carrying out vortex and centrifugation, performing separating to obtain the supernatant, adding an eluent, uniformly performing mixing, performing centrifuging, evaporating the supernatant to dryness, and performing redissolving with an ammonium formate aqueous solution to obtain a to-be-detected solution; using blank goat milk to obtain to-be-detected solution containing the sulfanilamide medicine standard substance, and analyzing the two solutions to obtain quantitative ion chromatographic peaks, the corresponding retention time, a molecular ion mass-to-charge ratio and a fragment ion mass-to-charge ratio; and finally, comparing the information to obtain the peak area of the quantitative ion chromatographic peak of each sulfonamide drug, and calculating the concentration of each sulfonamide drug in the goat milk in combination with a matrix matching mixed standard working curve.

Description

technical field [0001] The invention belongs to the technical field of food safety detection, in particular to a method for screening sulfonamide drug residues in goat milk. Background technique [0002] Sulfonamide antibiotics are antibiotics containing p-aminobenzenesulfonamide structure, which play an irreplaceable role in the development of animal husbandry, because they can promote animal growth, improve feed conversion rate, prevent and treat various diseases, reduce mortality, and improve animal product quality However, the unreasonable use of these drugs, such as overdose or abuse, will cause poisoning in sensitive populations, accumulate toxicity, and even produce drug resistance, which will bring harm to human health. [0003] Existing multi-residue detection methods include "SN / T 2624-2010 Determination of Multiple Basic Drug Residues in Foods of Animal Origin Liquid Chromatography-Mass Spectrometry / Mass Spectrometry", "SN / T 2443-2010 Import and Export of Animal S...

Claims

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Application Information

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IPC IPC(8): G01N30/96G01N30/06G01N30/72G01N30/86
CPCG01N30/96G01N30/06G01N30/72G01N30/8634
Inventor 贾玮杜安樊子便
Owner SHAANXI UNIV OF SCI & TECH
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