Multi-mode probe FN, and preparation method and application thereof
A multimodal, probe technology, applied in the fields of hydrazone preparation, organic chemistry methods, chemical instruments and methods, etc., can solve the problems of non-invasive in vivo imaging of the unfavorable nervous system, limited applications, etc., and achieve high sensitivity, specificity, and selection good effect
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Embodiment 1
[0046] The reaction chemical equation of the multimodal probe FN is as follows:
[0047]
[0048] It was prepared by the following steps: add 0.32mmol compound 1, 0.336mmol compound 2 and 3mL methanol to a 10mL flask, stir the mixture at room temperature for 8 hours, add 10mL water, store at -4°C, refrigerate for 5h, and filter. After filtration, the obtained solid was washed with ice water and ice methanol respectively, and then recrystallized to obtain yellow needle-like crystal 3 (multimodal probe FN), with a yield of 94%. 1 H NMR (400MHz, DMSO-d 6 )δ12.85(s,1H),10.95(s,1H),9.88(s,1H),9.02(s,1H),8.60(d,J=8.4Hz,1H),8.03(d,J=8.9 Hz,1H),7.90(d,J=7.6Hz,1H),7.63–7.54(m,2H),7.47–7.40(m,1H),7.34–7.23(m,2H),7.01(dd,J= 9.0,4.6Hz,1H).HRMS calculated for C 18 h 13 FN 2 o 2 (M+H + ): 308.0956, found 309.1038.
[0049] The multimodal probe FN obtained above is tested as follows:
[0050] Prepare the detection solution: dissolve the multimodal probe FN in tetrahydrofuran to o...
Embodiment 2
[0079] Isotopologue compounds of the multimodal probe FN[ 18 F]FN is prepared by including the following steps: 5- 18 F salicylaldehyde, the 5- 18 F salicylaldehyde and 2-hydroxynaphthaldehyde hydrazone react to obtain compound [ 18 F] FN.
[0080] The reaction chemical equation of the labeling precursor is as follows:
[0081]
[0082] Specifically include the following steps: add 0.81mmol 5-iodosalicylaldehyde (compound 4), 3.42mmol N,N-diisopropylethylamine and 4mL dichloromethane to a dry 25mL flask, add 1.21mmol chloromethyl Methyl ether, reacted at room temperature for 1 hour. Subsequently, the reaction system was quenched with 10 mL of saturated sodium bicarbonate, and then extracted with dichloromethane to obtain the dichloromethane extract phase, which was washed with saturated brine, dried over anhydrous magnesium sulfate, filtered to remove the solid, and removed under reduced pressure. Dichloromethane, and the residue was purified by column chromatography (...
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