A sterile operation device for stem cell preparation

Abstract

The invention relates to the technical field of stem cell preparation, and provides a sterile operation device for stem cell preparation, comprising a base and an operation box fixedly mounted on the base, wherein an inlet and outlet groove is provided at the bottom of one end of the base, a rising groove communicating with the inlet and outlet groove is provided at the middle position of the top of the base, an inlet and outlet communicating with the rising groove is provided at the middle position of the bottom of the operation box, a side groove is provided at one end of the rising groove, and a ventilation cavity is provided inside the top corner of the base; a placing frame is arranged to fit inside the inlet and outlet groove. The invention ensures that the operation area is in a sterile environment, is convenient for taking and placing stem cell storage containers, is convenient for replacing sterile air in a small ventilation area, avoids the problem of long-term contact and contamination of stem cells with bacteria, and hinders the entry of sterile air into the operation device, avoids the contact of stem cells with bacteria at the first time.

Description

Technical Field

[0001] The present invention relates to the technical field of stem cell preparation, and in particular to a sterile operation device for stem cell preparation. Background Art

[0002] Stem cells are a type of multipotent cells with the ability to self-replicate. Under certain conditions, they can differentiate into multiple functional cells. According to the developmental stage of stem cells, they are divided into embryonic stem cells and adult stem cells. Stem cells are not fully differentiated and immature cells that have the potential to regenerate various tissues, organs and the human body. They are called "universal cells" in the medical field. At present, in the process of stem cell preparation, sterile operating devices are required for production. Sterile operating tables are a necessary equipment in the field of biomedical research. They can provide a sterile clean space for scientific researchers' experimental operations.

[0003] After searching, the patent publication number CN213791692U is a sterile operation device for stem cell preparation, including a support column, a sterile base, an operation shell, a sliding door, a perspective window, a circulating bellows, a control panel, a pressure indicator, an air inlet, a connecting socket and an ejection device. The utility model is provided with an ejection device at the rear end of the operation shell, and the electric push rod pushes the moving plate forward, and the moving plate drives the wire mesh to move out of the operation shell through the square frame. At the same time, the moving plate drives the slide rod to slide inside the slide seat, and the limit plate plays a role of limiting, and then plays a role of stable movement. Then the external glassware is placed on the upper end of the wire mesh, and then the electric push rod pulls the moving plate to be stored in the operation shell, thereby facilitating the taking of the glassware, ensuring that the hands are not burned and the use of the device is not affected.

[0004] In combination with the above patents, it is found that the prior art has the following deficiencies: when placing a stem cell storage container, bacteria in the air will remain inside the opening, causing the stem cells to come into contact with the bacteria for the first time, causing stem cell contamination problems; on the other hand, it cannot ensure that the operating area inside the device is in a sealed environment, causing bacteria in the air to enter the operating area of ​​the device along the opening, also causing stem cell contamination problems. Although the air in the operating area is replaced by a circulating bellows, the gas replacement speed is slow due to the large space in the operating area, causing the stem cells to come into contact with bacteria for a long time, further affecting the work efficiency of stem cell preparation. Therefore, it is urgent to design a sterile operation device for stem cell preparation to solve the above problems. Summary of the invention

[0005] The present invention provides a sterile operation device for stem cell preparation, aiming to solve the problem that when placing a stem cell storage container, bacteria in the air remain inside an opening and enter the operating area of ​​the device along the opening, causing stem cell contamination.

[0006] The present invention is implemented as follows: a sterile operation device for stem cell preparation, comprising a base and an operation box fixedly mounted on the base, an inlet and outlet groove is provided at the bottom of one end of the base, a rising groove communicating with the inlet and outlet groove is provided at the middle of the top of the base, an inlet and outlet communicating with the rising groove is provided at the middle of the bottom of the operation box, a side groove is provided at one end of the rising groove, and a ventilation cavity is provided inside the top corner of the base;

[0007] The receiving frame is arranged to fit inside the inlet and outlet grooves, the outer walls of the receiving frame are precisely fitted with the inner walls of the ascending grooves, and the inner walls of the receiving frame are fixedly installed with support nets;

[0008] A right-angle turning component, which is arranged inside the inlet and outlet slot, inside the side slot and between one end of the receiving frame;

[0009] A displacement mechanism, the displacement mechanism is arranged at the bottom of the right-angle turn assembly;

[0010] A ventilation component, the ventilation component is arranged inside the ventilation cavity;

[0011] A sealing assembly is arranged between the interior of the operation box and the interior of the inlet and outlet.

[0012] Furthermore, an observation port is provided on one side of the operation box, and a transparent plate is fixedly installed on the inner wall of the observation port, the transparent plate is sealedly connected to the observation port, a detachable port is provided on the transparent plate, and a transparent cover is fixedly installed on the inner wall of the detachable port, the transparent cover is sealedly connected to the detachable port, two operation ports are provided on the transparent cover, and protective operation gloves are fixed on the inner walls of the two operation ports, so as to perform observation operations on stem cell preparation and form contactless preparation operation performance, thereby avoiding contamination of the user's hands and contamination of stem cells.

[0013] Furthermore, the right-angle turn component includes an extension outlet opened between the bottom of the side groove and the outer wall at the other end of the base, and the extension outlet is communicated with the inlet and outlet grooves, a movable long board is movably arranged at the bottom middle position of the inlet and outlet groove and inside the extension outlet, and an anti-stuck groove is opened at one end of the top middle position of the movable long board, and an equidistantly distributed tooth groove is opened at the other end of the top middle position of the movable long board, a large gear is rotatably connected to the bottom end of the inner wall of the side groove, and the bottom of the large gear is in meshing state with the tooth groove, the bottom and top of the inner wall of the side groove are rotatably connected to transmission rollers, the outer wall of the transmission roller is transmission-connected to the conveyor belt, a small gear is fixed at the middle position of the transmission roller at the bottom, and the small gear and the large gear are meshed. The wheels are meshed with each other, a through groove for the movement of the small gear is opened in the middle position of the conveyor belt, a rising opening is opened between the top side of the side groove and the bottom side of the operation box, and an inverted T-shaped lifting plate inserted in the rising opening is fixedly installed at the bottom of one side of the conveyor belt, and the outer wall of the inverted T-shaped lifting plate forms a precise fit with the inner wall of the rising opening and the inner wall of the side groove, a positioning groove is opened at the bottom of one end of the inverted T-shaped lifting plate, and a positioning block that is snap-fitted with the positioning groove is fixed at the bottom of one end of the receiving frame, so as to realize the operation mode of horizontal movement and then vertical movement of the receiving frame, and the sealing cover is separated from the inlet and outlet, so that the top of the receiving frame is moved out of the inlet and outlet, which is convenient for the aseptic preparation operation of stem cells.

[0014] Furthermore, the displacement mechanism includes a displacement groove opened in the middle position of the bottom of the inlet and outlet groove and the middle position of the bottom of the extension outlet, and a threaded rod is rotatably connected between the inner walls at both ends of the displacement groove, and a forward and reverse motor for driving the threaded rod to rotate is fixedly installed at the bottom end of one end of the base, a movable sleeve is screwed on the outer wall of the threaded rod, and the top of the movable sleeve is fixedly connected to the bottom of the movable long board, L-shaped displacement plates are fixed on both sides of the movable long board, and L-shaped openings for the L-shaped displacement plates to pass through are opened between the two sides of one end of the inlet and outlet groove and the outer wall of the other end of the base, and the L-shaped opening is connected to the extension outlet, and a placement groove is opened on one side of the top of the two L-shaped displacement plates, and a placement frame is fitted inside the two placement grooves, and a reinforcement component is arranged between the bottom of the placement frame and the placement groove, and the threaded rod is driven to rotate by the forward and reverse motor, and the movable sleeve is driven to move on the threaded rod, thereby facilitating the right-angle turning component to work.

[0015] Furthermore, the reinforcement component includes a lower slot opened at the bottom of the placement groove and upper slots opened on both sides of the bottom of the receiving frame, and the inner wall of the lower slot is fixed with a lower magnetic strip, and the inner wall of the upper slot is fixed with an upper magnetic strip. The upper magnetic strip is adsorbed with the lower magnetic strip to fix the receiving frame on the L-shaped displacement plate to ensure the stability of the receiving frame during horizontal movement.

[0016] Furthermore, an L-shaped guide groove is opened between the middle positions on both sides of the entry and exit groove and the middle positions on both sides of the rising groove, and guide blocks inserted in the L-shaped guide groove are fixed on both sides of the receiving frame. The outer wall of the guide block forms a precise fit with the inner wall of the L-shaped guide groove, thereby ensuring the stable performance of the receiving frame during movement.

[0017] Furthermore, the ventilation assembly includes a mounting plate fixedly mounted on the inner wall of the ventilation chamber, and a sterilizer is fixedly mounted on one side of the top of the mounting plate, a first three-way pipe is fixedly mounted on the top of the other end of the rising groove and the interior of the ventilation chamber, and one end of the first three-way pipe extends from the interior of the ventilation chamber to the outside of the base, a one-way valve is fixedly mounted on one side of the first three-way pipe close to the rising groove, the bottom end of the first three-way pipe is fixedly connected to the air inlet end of the sterilizer, an exhaust fan is fixedly mounted on the other side of the top of the mounting plate, and the air inlet end of the exhaust fan is fixedly connected to the air outlet end of the sterilizer, and a bottom corner of the ventilation chamber is fixedly mounted. A second three-way pipe is fixedly installed, and one end of the second three-way pipe is communicated with the interior of the rising groove, the bottom end of the second three-way pipe is communicated with the interior of the inlet and outlet groove, the other end of the second three-way pipe is fixedly connected to the air outlet end of the exhaust fan, and an electromagnetic three-way valve is fixedly installed in the middle position of the second three-way pipe, so that sterile air is filled in the inlet and outlet starting ends of the storage container, so that the sterile air is hindered from entering the operating device, avoiding the contamination of stem cells by contacting with bacteria at the first time, and also realizing the ventilation operation of sterile air in the ventilation area, ensuring that the air entering the operating box is in a sterile state.

[0018] Furthermore, the sealing assembly includes a sealing cover that is attached to the inner wall of the inlet and outlet, and a T-shaped lifting plate is fixed on the top of the sealing cover, guide columns are fixed at both ends of the inner wall of the operating box, and guide holes for the guide columns to pass through are fixed at both ends of the T-shaped lifting plate, springs mounted on the outside of the guide columns are fixed between the top ends of the T-shaped lifting plate and the top inner wall of the operating box, and ultraviolet sterilization lamps are fixedly installed on both sides of the T-shaped lifting plate to ensure that the interior of the operating box is in a sealed environment, to prevent bacteria in the air from entering the interior of the operating box along the inlet and outlet grooves and the rising groove, and to sterilize the interior of the operating box by the ultraviolet sterilization lamp, so that the interior of the operating box has a sterilization function.

[0019] Furthermore, a first infrared distance sensor is fixedly installed in an inclined shape at the corner of the top inner wall of the inlet and outlet groove, and a mounting groove is opened in the middle position of the bottom of the sealing cover, and a second infrared distance sensor is fixedly installed on the inner wall of the mounting groove. The second infrared distance sensor and the first infrared distance sensor both correspond to the position of the receiving frame. A controller is fixedly installed on the top of one side of the operation box, and the controller is electrically connected to the first infrared distance sensor, the second infrared distance sensor, and the electromagnetic three-way valve. The controller is also electrically connected to the forward and reverse motor, the sterilizer and the exhaust fan, and the ultraviolet sterilization lamp to control the electromagnetic three-way valve for steering processing, so as to facilitate the adjustment of the exhaust position of the sterile air.

[0020] Furthermore, an inspection port is provided between the inner wall at one end of the ventilation chamber and the outer wall at one end of the base, and an inspection cover is fixedly installed on the inner wall of the inspection port. Universal wheels are fixedly installed on the four bottom corners of the base to facilitate the inspection and maintenance of the equipment in the ventilation chamber and to facilitate the movement of the entire operating device.

[0021] After adopting the above structure, the present invention has the following advantages compared with the prior art:

[0022] First, the interior of the operating box is always in a sealed environment, preventing bacteria in the air from entering the interior of the operating box along the inlet and outlet grooves and the rising groove, and making the interior of the operating box have a sterilization function, ensuring that the operating area of ​​the entire operating device is in a sterile environment, thereby improving the operating efficiency of the entire device;

[0023] Secondly, the stem cell storage container of the present invention can form a horizontal and vertical reciprocating motion operation mode inside the entire operation device, which can facilitate the placement and removal of the stem cell storage container while achieving a sealed environment in the operation box, and is easy to operate without manual material removal;

[0024] Thirdly, when the placement frame is moved into the ascending groove, the present invention forms a relatively smaller ventilation area through the placement frame, the ascending groove and the sealing cover, so that the small ventilation area is replaced with sterile air, so that the gas replacement speed is faster, and the sterile air enters the operation box along with the placement frame, avoiding the problem of long-term contact and bacterial operation contamination of stem cells, and further improving the work efficiency of stem cell preparation;

[0025] Fourthly, the present invention can convert the exhaust direction of the ventilation component. When taking and storing the stem cell storage container, the sterile air can be released inside the inlet and outlet grooves, so that the sterile air fills the inlet and outlet starting ends of the storage container, thereby hindering the entry of contaminated air into the operating device. On the one hand, it prevents the contaminated air from being retained at one end of the inlet and outlet grooves, avoiding the problem of contamination caused by the first contact of stem cells with bacteria. On the other hand, it can blow air to dissipate heat for the stem cell storage container taken out after the operation, ensuring that there will be no scalding problems when taking the container, thereby achieving the effect of conveniently taking the stem cell storage container and improving the working performance. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the invention and, together with the description, serve to explain the principles of the invention.

[0027] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative labor.

[0028] Figure 1 It is a schematic diagram of the overall structure of the present invention.

[0029] Figure 2 It is an overall cross-sectional view of the present invention.

[0030] Figure 3 It is a cross-sectional view of the base and the operating box of the present invention.

[0031] Figure 4 It is a schematic diagram of the cross-sectional structure of the base of the present invention.

[0032] Figure 5 It is a schematic diagram of the structure of the displacement mechanism and the right-angle turning assembly of the present invention.

[0033] Figure 6 It is a schematic diagram of the structure of the placement slot and the lower magnetic attraction strip of the present invention.

[0034] Figure 7 It is a schematic diagram of the upper magnetic attraction strip and positioning block structure of the present invention.

[0035] Figure 8 It is a schematic diagram of the positioning groove structure of the present invention.

[0036] Fig. 9 It is a schematic diagram of the sealing cover and the second distance sensor structure of the present invention.

[0037] Fig.10 It is a schematic diagram of the structure of the ventilation component of the present invention.

[0038] Fig.11 It is a side view of the transparent cover and protective operating gloves of the present invention.

[0039] Reference numerals:

[0040] 1. Base; 2. Displacement mechanism; 201. Displacement slot; 202. L-shaped through port; 203. Forward and reverse motor; 204. Threaded rod; 205. Movable sleeve; 206. L-shaped displacement plate; 3. Inlet and outlet slot; 4. Inspection cover; 5. Universal wheel; 6. Operation box; 7. Controller; 8. Transparent plate; 9. Transparent cover; 10. Protective operating gloves; 11. Rising slot; 12. Ventilation chamber; 13. Side slot; 14. Right-angle turning assembly; 1401. Extension outlet; 1402. Rising port; 1403. Movable long board; 1404. Anti-stuck slot; 1405. Large gear; 1406. Small gear; 1407. Conveyor belt; 1408. Through slot; 1409. Inverted T-shaped lifting plate; 1 410, tooth groove; 1411, placement groove; 1412, lower magnetic strip; 1413, upper magnetic strip; 1414, positioning block; 1415, positioning groove; 15, placement frame; 16, support net; 17, L-shaped guide groove; 18, ventilation component; 1801, mounting plate; 1802, sterilizer; 1803, first three-way pipe; 1804, one-way valve; 1805, exhaust fan; 1806, second three-way pipe; 1807, electromagnetic three-way valve; 19, sealing cover; 20, T-shaped lifting plate; 21, guide column; 22, spring; 23, ultraviolet sterilization lamp; 24, first infrared distance sensor; 25, inlet and outlet; 26, guide block; 27, second infrared distance sensor. DETAILED DESCRIPTION

[0041] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0042] In order to effectively illustrate the embodiments of the present invention, the embodiments of the present application are described in detail below with reference to the accompanying drawings. Example

[0043] Reference Figure 1-Figure 11 , a sterile operation device for stem cell preparation, comprising a base 1 and an operation box 6 fixedly mounted on the base 1, an inlet and outlet groove 3 is provided at the bottom of one end of the base 1, a rising groove 11 communicating with the inlet and outlet groove 3 is provided at the middle position of the top of the base 1, an inlet and outlet 25 communicating with the rising groove 11 is provided at the middle position of the bottom of the operation box 6, a side groove 13 is provided at one end of the rising groove 11, and a ventilation cavity 12 is provided inside the top corner of the base 1;

[0044] The receiving frame 15 is arranged to fit inside the inlet and outlet groove 3, and the outer walls of the receiving frame 15 are precisely fitted with the inner walls of the ascending groove 11. The inner walls of the receiving frame 15 are fixedly installed with a supporting net 16, which is precisely fitted between the receiving frame 15, the inlet and outlet groove 3 and the ascending groove 11 to form a nearly completely sealed working environment;

[0045] The right-angle turn component 14 is arranged inside the inlet and outlet groove 3, inside the side groove 13 and between one end of the receiving frame 15. The right-angle turn component 14 includes an extension port 1401 opened between the bottom of the side groove 13 and the outer wall of the other end of the base 1, and the extension port 1401 is communicated with the inlet and outlet groove 3. A movable long plate 1403 is movably arranged in the middle position of the bottom of the inlet and outlet groove 3 and the interior of the extension port 1401, and an anti-stuck groove 1404 is opened at one end of the middle position of the top of the movable long plate 1403, and a tooth groove 1410 distributed at equal distances is opened at the other end of the middle position of the top of the movable long plate 1403. The bottom end of the inner wall of the side groove 13 is rotatably connected with a large gear 1405, and the bottom of the large gear 1405 is in meshing state with the tooth groove 1410. The bottom and top of the inner wall of the groove 13 are rotatably connected with a transmission roller, and the outer wall of the transmission roller is transmission-connected with a conveyor belt 1407, a small gear 1406 is fixed in the middle position of the transmission roller at the bottom, and the small gear 1406 is meshed with the large gear 1405, and a through groove 1408 for the small gear 1406 to move is provided in the middle position of the conveyor belt 1407, a rising port 1402 is provided between the top side of the side groove 13 and the bottom side of the operation box 6, and an inverted T-shaped lifting plate 1409 inserted in the rising port 1402 is fixedly installed at the bottom of one side of the conveyor belt 1407, the outer wall of the inverted T-shaped lifting plate 1409 forms a precise fit with the inner wall of the rising port 1402 and the inner wall of the side groove 13, and a through groove 1408 is provided at the bottom of one end of the inverted T-shaped lifting plate 1409. The positioning groove 1415 is provided, and a positioning block 1414 is fixed at the bottom of one end of the receiving frame 15 to form a snap connection with the positioning groove 1415. When working, the movable long plate 1403 and the L-shaped displacement plate 206 as well as the receiving frame 15 and the stem cell storage container will move horizontally. When the tooth groove 1410 on the movable long plate 1403 meshes with the large gear 1405, the positioning block 1414 will gradually be inserted into the positioning groove 1415, and at this time, the large gear 1405 will be driven to rotate, thereby cooperating with the meshing action of the large gear 1405 and the small gear 1406 to drive the conveyor belt 1407 to rotate, and drive the inverted T-shaped lifting plate 1409 to move upward, thereby driving the receiving frame 15 and the stem cell storage container to move upward. When the receiving frame 15 gradually moves into the rising groove 11, the rising groove 11 will remain sealed under the precise fit of the sealing cover 19 of the sealing assembly, the receiving frame 15 and the inverted T-shaped lifting plate 1409, and a relatively smaller ventilation area is formed by the receiving frame 15, the rising groove 11, the sealing cover 19 and the inverted T-shaped lifting plate 1409, so as to replace the sterile air in the ventilation area of ​​the small area, and then continue to drive the inverted T-shaped lifting plate 1409 and the receiving frame 15 to move upward under the driving action of the conveyor belt 1407, and use the inverted T-shaped lifting plate 1409 to push the T-shaped lifting plate 20 in the sealing assembly to move upward, so that the sealing cover 19 is separated from the inlet and outlet 25, and the top of the receiving frame 15 is moved out of the inlet and outlet 25 and stops moving, so as to facilitate the aseptic preparation operation of stem cells;

[0046] The displacement mechanism 2 is arranged at the bottom of the right-angle turn component 14, and the displacement mechanism 2 includes a displacement groove 201 opened at the middle position of the bottom of the inlet and outlet groove 3 and the middle position of the bottom of the extension outlet 1401, and a threaded rod 204 is rotatably connected between the inner walls at both ends of the displacement groove 201, and a forward and reverse motor 203 for driving the threaded rod 204 to rotate is fixedly installed at the bottom end of one end of the base 1, and a movable sleeve 205 is screwed on the outer wall of the threaded rod 204, and the top of the movable sleeve 205 is fixedly connected to the bottom of the movable long board 1403, and L-shaped displacement plates 206 are fixed on both sides of the movable long board 1403, and L-shaped openings 202 for L-shaped displacement plates 206 to pass through are opened between the two sides of one end of the inlet and outlet groove 3 and the outer wall of the other end of the base 1, and the L-shaped opening 202 is communicated with the extension outlet 1401, and a placement groove 1411 is opened on one side of the top of the two L-shaped displacement plates 206, and the placement groove 1411 is opened on one side of the top of the two L-shaped displacement plates 206. The receiving frame 15 fits inside the two placement grooves 1411, and a reinforcement component is arranged between the bottom of the receiving frame 15 and the placement grooves 1411. The reinforcement component includes a lower notch opened at the bottom of the placement groove 1411 and upper notches opened at both sides of the bottom of the receiving frame 15, and the inner wall of the lower notch is fixed with a lower magnetic attraction bar 1412, and the inner wall of the upper notch is fixed with an upper magnetic attraction bar 1413, and the upper magnetic attraction bar 1413 is adsorbed with the lower magnetic attraction bar 1412. The receiving frame 15 is fixed on the L-shaped displacement plate 206 by the adsorption and fixing effect of the lower magnetic attraction bar 1412 and the upper magnetic attraction bar 1413 in the reinforcement component, so as to ensure the stability of the receiving frame 15 during horizontal movement, and start the forward and reverse motor 203 in the displacement mechanism 2, and the threaded rod 204 is driven to rotate by the forward and reverse motor 203, so as to drive the movable sleeve 205 to move on the threaded rod 204, thereby driving the right-angle turning component 14 to work;

[0047] The ventilation assembly 18 is arranged inside the ventilation chamber 12. The ventilation assembly 18 includes a mounting plate 1801 fixedly mounted on the inner wall of the ventilation chamber 12, and a sterilizer 1802 is fixedly mounted on one side of the top of the mounting plate 1801. A first three-way pipe 1803 is fixedly mounted on the top of the other end of the riser 11 and the inside of the ventilation chamber 12, and one end of the first three-way pipe 1803 extends from the inside of the ventilation chamber 12 to the outside of the base 1. The first three-way pipe 1803 is fixedly mounted on one side of the riser 11. There is a one-way valve 1804, the bottom end of the first three-way pipe 1803 is fixedly connected to the air inlet end of the sterilizer 1802, an exhaust fan 1805 is fixedly installed on the other side of the top of the mounting plate 1801, and the air inlet end of the exhaust fan 1805 is fixedly connected to the air outlet end of the sterilizer 1802, a second three-way pipe 1806 is fixedly installed at the bottom corner of the ventilation cavity 12, and one end of the second three-way pipe 1806 is communicated with the interior of the rising groove 11, and the bottom end of the second three-way pipe 1806 is communicated with the interior of the inlet and outlet groove 3, and the second The other end of the second three-way pipe 1806 is fixedly connected to the air outlet end of the exhaust fan 1805, and an electromagnetic three-way valve 1807 is fixedly installed in the middle position of the second three-way pipe 1806. The exhaust fan 1805 is used to introduce the outside air into the sterilizer 1802 along the first three-way pipe 1803, and the sterilization function of the sterilizer 1802 is used to sterilize the outside air, so that the sterilized air is discharged into the inside of the inlet and outlet groove 3 along the bottom end of the second three-way pipe 1806, so that the sterile air fills the storage container. The inlet and outlet start end prevents the sterile air from entering the operating device, preventing the stem cells from coming into contact with bacteria and causing contamination in the first place, and also allows the sterile air to enter the interior of the rising tank 11 along one end of the second three-way pipe 1806, and then the air inside the rising tank 11 is discharged out of the base 1 along one end of the first three-way pipe 1803 and enters the sterilizer 1802 for continued sterilization, thereby realizing the ventilation operation of the ventilation area with sterile air, ensuring that the air entering the operating box 6 is in a sterile state;

[0048] A sealing component is arranged between the interior of the operation box 6 and the interior of the inlet and outlet 25. The sealing component includes a sealing cover 19 that is attached to the inner wall of the inlet and outlet 25, and a T-shaped lifting plate 20 is fixed on the top of the sealing cover 19. Guide columns 21 are fixed at both ends of the inner wall of the operation box 6, and guide holes for the guide columns 21 to pass through are fixed at both ends of the top of the T-shaped lifting plate 20. Springs 22 that are sleeved on the outside of the guide columns 21 are fixed between the top ends of the T-shaped lifting plate 20 and the top inner wall of the operation box 6. Ultraviolet sterilization lamps 23 are fixedly installed on both sides of the T-shaped lifting plate 20. The compression performance of the spring 22 in the sealing component makes the sealing cover 19 tightly attached to the inlet and outlet 25, ensuring that the interior of the operation box 6 is always in a sealed environment, preventing bacteria in the air from entering the interior of the operation box 6 along the inlet and outlet grooves 3 and the rising grooves 11, and sterilizing the interior of the operation box 6 by the ultraviolet sterilization lamp 23, so that the interior of the operation box 6 has a sterilization function, ensuring that the operating area of ​​the entire operating device is in a sterile environment, and improving the operating efficiency of the entire device.

[0049] In the present invention, an observation port is provided on one side of the operation box 6, and a transparent plate 8 is fixedly installed on the inner wall of the observation port, and the transparent plate 8 is sealedly connected to the observation port. A detachable port is provided on the transparent plate 8, and a transparent cover 9 is fixedly installed on the inner wall of the detachable port, and the transparent cover 9 is sealedly connected to the detachable port. Two operation ports are provided on the transparent cover 9, and protective operation gloves 10 are fixed to the inner walls of the two operation ports. The observation operation of stem cell preparation is performed through the transparent plate 8 and the transparent cover 9, and the operation is performed through the protective operation gloves 10, so as to form a contactless preparation operation performance, and avoid the problem of contamination of the user's hands and contamination of stem cells.

[0050] In the present invention, a first infrared distance sensor 24 is fixedly installed at the corner of the top inner wall of the inlet and outlet groove 3, and a mounting groove is opened at the middle position of the bottom of the sealing cover 19, and a second infrared distance sensor 27 is fixedly installed on the inner wall of the mounting groove. The second infrared distance sensor 27 and the first infrared distance sensor 24 correspond to the position of the receiving frame 15. A controller 7 is fixedly installed on the top of one side of the operation box 6, and the controller 7 is electrically connected to the first infrared distance sensor 24, the second infrared distance sensor 27, and the electromagnetic three-way valve 1807. The controller 7 is also electrically connected to the forward and reverse motor 203, the sterilizer 1802, the exhaust fan 1805 and the ultraviolet sterilization lamp 23. When the first infrared distance sensor 24 moves horizontally, it is always affected by the distance of the receiving frame 15 to keep the electromagnetic three-way valve 1807 working in the same direction, thereby controlling the sterile air in the ventilation assembly 18 to enter the initial end of the inlet and outlet groove 3 along the bottom end of the second three-way pipe 1806. When the receiving frame 15 moves vertically and gradually moves into the ascending groove 11, the first infrared distance sensor 24 will be separated from the receiving frame 15, and the second infrared distance sensor 27 will be close to the receiving frame 15. The first infrared distance sensor 24 is controlled to be turned off and the second infrared distance sensor 27 is controlled to be started through the controller 7, thereby controlling the electromagnetic three-way valve 1807 to perform a steering process, so as to facilitate the adjustment of the exhaust position of the sterile air.

[0051] In the present invention, an inspection port is provided between the inner wall at one end of the ventilation chamber 12 and the outer wall at one end of the base 1, and an inspection cover 4 is fixedly installed on the inner wall of the inspection port, and universal wheels 5 are fixedly installed at the four corners of the bottom of the base 1. The inspection cover 4 facilitates the inspection and maintenance of the equipment in the ventilation chamber 12, and the universal wheels 5 also facilitate the movement of the entire operating device. Example

[0052] Reference Figure 3 and Figure 5 An L-shaped guide groove 17 is provided between the middle positions on both sides of the inlet and outlet grooves 3 and the middle positions on both sides of the rising grooves 11, and guide blocks 26 inserted into the L-shaped guide grooves 17 are fixed on both sides of the receiving frame 15. The outer wall of the guide block 26 forms a precise fit with the inner wall of the L-shaped guide groove 17. When the receiving frame 15 moves horizontally and vertically, the guide block 26 always moves in the L-shaped guide groove 17 to ensure the stable performance of the receiving frame 15 during movement.

[0053] In summary, the working principle of the present invention is as follows: when in use, the receiving frame 15 is located at the inlet and outlet starting end of the inlet and outlet slot 3, and the receiving frame 15 is fixed on the L-shaped displacement plate 206 by the adsorption and fixing effect of the lower magnetic strip 1412 and the upper magnetic strip 1413 in the reinforcement component. The user first starts the sterilizer 1802, the exhaust fan 1805, and the ultraviolet sterilization lamp 23 through the controller 7, and uses the ultraviolet sterilization lamp 23 to sterilize the interior of the operation box 6 to ensure that the operation box 6 is in a sterile environment, and then uses the exhaust fan 1805 to introduce the outside air into the sterilizer 1802 along the first three-way pipe 1803, and the outside air is sterilized by the sterilization function of the sterilizer 1802, so that the sterilized air is discharged along the second three-way pipe 1806. The bottom end is discharged into the interior of the inlet and outlet groove 3, so that sterile air fills the inlet and outlet start end of the storage container, so that the contaminated air is blocked from entering the operating device, and the stem cells are prevented from contacting with bacteria and causing contamination at the first time. Next, the user places the stem cell storage container on the support net 16 in the receiving frame 15, and starts the forward and reverse motor 203 in the displacement mechanism 2 through the controller 7. The forward and reverse motor 203 drives the threaded rod 204 to rotate, and drives the movable sleeve 205 to move on the threaded rod 204, thereby driving the movable long plate 1403 and the L-shaped displacement plate 206 in the right-angle turning component 14, as well as the receiving frame 15 and the stem cell storage container to move horizontally. At this time, the first infrared distance sensor 24 is always affected by the distance effect of the receiving frame 15 to keep the power on. The magnetic three-way valve 1807 works in the same direction. When the tooth groove 1410 on the movable long plate 1403 meshes with the large gear 1405, the positioning block 1414 will gradually be inserted into the positioning groove 1415. At this time, the continuous movement of the displacement mechanism 2 will drive the large gear 1405 to rotate, thereby cooperating with the meshing action of the large gear 1405 and the small gear 1406 to drive the conveyor belt 1407 to rotate, and drive the inverted T-shaped lifting plate 1409 to move upward, thereby driving the receiving frame 15 and the stem cell storage container to move upward. When the receiving frame 15 gradually moves into the rising groove 11, the rising groove 11 will remain sealed under the precise fit of the sealing cover 19 of the sealing assembly, the receiving frame 15 and the inverted T-shaped lifting plate 1409. 11 and the sealing cover 19 and the inverted T-shaped lifting plate 1409 form a relatively smaller ventilation area. At this time, the first infrared distance sensor 24 will be separated from the receiving frame 15, and the second infrared distance sensor 27 will be close to the receiving frame 15. The first infrared distance sensor 24 is controlled to be closed by the controller 7, and the second infrared distance sensor 27 is controlled to be started, so as to control the electromagnetic three-way valve 1807 to perform steering processing, and the sterile air is replaced in the ventilation area of ​​the small area. Then, the inverted T-shaped lifting plate 1409 and the receiving frame 15 are continued to be driven by the conveyor belt 1407 to move upward, and the T-shaped lifting plate 20 in the sealing assembly is driven to move upward, so that the sealing cover 19 is separated from the inlet and outlet 25.When the top of the receiving frame 15 is moved out of the inlet and outlet 25, the forward and reverse motor 203 is stopped, and the user performs the aseptic preparation operation of the stem cells through the protective operation gloves 10; after the preparation is completed, the user starts the forward and reverse motor 203 to work in reverse, so that the above operation is reversed, thereby moving the receiving frame 15 and the stem cell storage dish to the initial end of the inlet and outlet slot 3, so that the sterile air discharged from the ventilation component 18 continues to blow at the initial end of the inlet and outlet slot 3, thereby blowing and cooling the taken out stem cell storage container, ensuring that there will be no scalding when taking the container.

[0054] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A sterile operation device for preparing stem cells, characterized in that: The invention comprises a base (1) and an operating box (6) fixedly mounted on the base (1), wherein an inlet and outlet groove (3) is provided at the bottom of one end of the base (1), an ascending groove (11) communicating with the inlet and outlet groove (3) is provided at the middle of the top of the base (1), an inlet and outlet (25) communicating with the ascending groove (11) is provided at the middle of the bottom of the operating box (6), a side groove (13) is provided at one end of the ascending groove (11), and a ventilation cavity (12) is provided inside the top corner of the base (1); A receiving frame (15), the receiving frame (15) being arranged to fit inside the inlet and outlet groove (3), the outer walls of the receiving frame (15) being precisely fitted with the inner walls of the ascending groove (11), and the inner walls of the receiving frame (15) being fixedly mounted with a support net (16); A right-angle turn component (14), the right-angle turn component (14) being arranged inside the inlet and outlet groove (3), inside the side groove (13) and between one end of the receiving frame (15), the right-angle turn component (14) comprising an extension outlet (1401) opened between the bottom of the side groove (13) and the outer wall of the other end of the base (1), and the extension outlet (1401) being communicated with the inlet and outlet groove (3), the middle position of the bottom of the inlet and outlet groove (3) and the internal movement of the extension outlet (1401) A movable long board (1403) is provided, and an anti-stuck groove (1404) is provided at one end of the middle position of the top of the movable long board (1403), and tooth grooves (1410) distributed at equal distances are provided at the other end of the middle position of the top of the movable long board (1403), and a large gear (1405) is rotatably connected to the bottom end of the inner wall of the side groove (13), and the bottom of the large gear (1405) is in meshing state with the tooth groove (1410), and the bottom and top of the inner wall of the side groove (13) are both rotatably connected A transmission roller is provided, and the outer wall of the transmission roller is connected to a conveyor belt (1407). A small gear (1406) is fixed in the middle of the transmission roller at the bottom, and the small gear (1406) is meshed with a large gear (1405). A through groove (1408) for the small gear (1406) to move is provided in the middle of the conveyor belt (1407). A rising opening (1402) is provided between the top side of the side groove (13) and the bottom side of the operating box (6), and the conveyor belt An inverted T-shaped lifting plate (1409) inserted into the rising opening (1402) is fixedly installed at the bottom of one side of (1407), and the outer wall of the inverted T-shaped lifting plate (1409) forms a precise fit with the inner wall of the rising opening (1402) and the inner wall of the side groove (13), and a positioning groove (1415) is provided at the bottom of one end of the inverted T-shaped lifting plate (1409), and a positioning block (1414) that is clamped with the positioning groove (1415) is fixed at the bottom of one end of the receiving frame (15); A displacement mechanism (2), the displacement mechanism (2) being arranged at the bottom of the right-angle turn assembly (14); A ventilation component (18), the ventilation component (18) being arranged inside the ventilation cavity (12); A sealing component is arranged between the interior of the operation box (6) and the interior of the inlet and outlet (25).

2. A sterile operation device for preparing stem cells according to claim 1, characterized in that: An observation port is provided on one side of the operation box (6), and a transparent plate (8) is fixedly mounted on the inner wall of the observation port, the transparent plate (8) being sealedly connected to the observation port; a detachable port is provided on the transparent plate (8), and a transparent cover (9) is fixedly mounted on the inner wall of the detachable port, the transparent cover (9) being sealedly connected to the detachable port; two operation ports are provided on the transparent cover (9), and protective operation gloves (10) are fixedly mounted on the inner walls of the two operation ports.

3. A sterile operation device for preparing stem cells according to claim 2, characterized in that: The displacement mechanism (2) comprises a displacement groove (201) provided at the middle position of the bottom of the inlet and outlet groove (3) and the middle position of the bottom of the extension outlet (1401), and a threaded rod (204) is rotatably connected between the inner walls at both ends of the displacement groove (201), a forward and reverse motor (203) for driving the threaded rod (204) to rotate is fixedly installed at the bottom end of one end of the base (1), a movable sleeve (205) is screwed on the outer wall of the threaded rod (204), and the top of the movable sleeve (205) is fixedly connected to the bottom of the movable long board (1403), and the movable long board (1403) is fixedly connected to the bottom of the movable long board (1403). L-shaped displacement plates (206) are fixed on both sides of the inlet and outlet groove (3) and the outer wall of the other end of the base (1), and L-shaped openings (202) for the L-shaped displacement plates (206) to pass through are provided between the two sides of one end of the inlet and outlet groove (3) and the outer wall of the other end of the base (1), and the L-shaped openings (202) are communicated with the extension outlet (1401), and a placement groove (1411) is provided on one side of the top of the two L-shaped displacement plates (206), and the receiving frame (15) is fitted inside the two placement grooves (1411), and a reinforcement component is provided between the bottom of the receiving frame (15) and the placement groove (1411).

4. A sterile operation device for preparing stem cells as claimed in claim 3, characterized in that: The reinforcement component comprises a lower notch opened at the bottom of the placement groove (1411) and upper notches opened at both sides of the bottom of the receiving frame (15), and the inner wall of the lower notch is fixed with a lower magnetic attraction strip (1412), and the inner wall of the upper notch is fixed with an upper magnetic attraction strip (1413), and the upper magnetic attraction strip (1413) is adsorbed with the lower magnetic attraction strip (1412).

5. A sterile operation device for preparing stem cells according to claim 4, characterized in that: An L-shaped guide groove (17) is provided between the middle positions on both sides of the entry and exit groove (3) and the middle positions on both sides of the rising groove (11), and guide blocks (26) inserted into the L-shaped guide groove (17) are fixed on both sides of the receiving frame (15), and the outer wall of the guide block (26) is precisely fitted with the inner wall of the L-shaped guide groove (17).

6. A sterile operation device for preparing stem cells according to claim 5, characterized in that: The ventilation assembly (18) includes a mounting plate (1801) fixedly mounted on the inner wall of the ventilation chamber (12), and a sterilizer (1802) is fixedly mounted on one side of the top of the mounting plate (1801), a first three-way pipe (1803) is fixedly mounted between the top of the other end of the rising groove (11) and the inside of the ventilation chamber (12), and one end of the first three-way pipe (1803) extends from the inside of the ventilation chamber (12) to the outside of the base (1), a one-way valve (1804) is fixedly mounted on one side of the first three-way pipe (1803) close to the rising groove (11), and the bottom end of the first three-way pipe (1803) is fixedly connected to the air inlet end of the sterilizer (1802). An exhaust fan (1805) is fixedly installed on the other side of the top of the mounting plate (1801), and the air inlet end of the exhaust fan (1805) is fixedly connected to the air outlet end of the sterilizer (1802), and a second three-way pipe (1806) is fixedly installed at the bottom corner of the ventilation chamber (12), and one end of the second three-way pipe (1806) is communicated with the interior of the rising groove (11), the bottom end of the second three-way pipe (1806) is communicated with the interior of the inlet and outlet groove (3), the other end of the second three-way pipe (1806) is fixedly connected to the air outlet end of the exhaust fan (1805), and an electromagnetic three-way valve (1807) is fixedly installed in the middle position of the second three-way pipe (1806).

7. A sterile operation device for preparing stem cells according to claim 6, characterized in that: The sealing assembly comprises a sealing cover (19) fitted on the inner wall of the inlet and outlet (25), and a T-shaped lifting plate (20) is fixed on the top of the sealing cover (19), guide pillars (21) are fixed on both ends of the inner wall of the operation box (6), and guide holes for the guide pillars (21) to pass through are fixed on both ends of the T-shaped lifting plate (20), springs (22) sleeved on the outside of the guide pillars (21) are fixed between both ends of the top of the T-shaped lifting plate (20) and the inner wall of the top of the operation box (6), and ultraviolet sterilization lamps (23) are fixedly installed on both sides of the T-shaped lifting plate (20).

8. The aseptic operation device for preparing stem cells according to claim 7, characterized in that: A first infrared distance sensor (24) is fixedly installed in an inclined shape at the corner of the top inner wall of the inlet and outlet groove (3), and a mounting groove is opened at the middle position of the bottom of the sealing cover (19), and a second infrared distance sensor (27) is fixedly installed on the inner wall of the mounting groove. The second infrared distance sensor (27) and the first infrared distance sensor (24) both correspond to the positions of the receiving frame (15). A controller (7) is fixedly installed on the top of one side of the operation box (6), and the controller (7) is electrically connected to the first infrared distance sensor (24), the second infrared distance sensor (27), and the electromagnetic three-way valve (1807). The controller (7) is also electrically connected to the forward and reverse motor (203), the sterilizer (1802), the exhaust fan (1805), and the ultraviolet sterilization lamp (23).

9. The aseptic operation device for preparing stem cells according to claim 8, characterized in that: An inspection opening is provided between an inner wall at one end of the ventilation cavity (12) and an outer wall at one end of the base (1), and an inspection cover (4) is fixedly mounted on the inner wall of the inspection opening. Universal wheels (5) are fixedly mounted on the four corners of the bottom of the base (1).

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