Cell treatment integrated method, system and medium, method for detecting cell viability and multi-channel control valve module

Abstract

The invention relates to the technical field of biomedical treatment, and discloses cell treatment integration, a method for detecting cell viability, a multi-channel control valve module, a cell treatment system and a storage medium. The cell treatment integration method comprises the following steps: connecting the multi-channel control valve module with a cell treatment integration module through a pipeline; sample liquid to be treated is obtained through the multi-channel control valve module; and separating and cleaning the obtained to-be-treated sample liquid through the cell treatment integrated module to obtain target cell liquid. Through the multi-channel control valve module and the cell treatment integrated module, a to-be-treated sample solution is automatically transferred, the cell treatment cycle is shortened, the cell treatment efficiency is improved, transfer of an operation site is avoided, the influence of external factors is reduced, it is ensured that the whole process is in a sterile environment, and the cell treatment efficiency is improved. Therefore, the culture efficiency and the qualification rate are improved.

Description

Technical Field

[0001] The present invention relates to the field of biomedical technology, and in particular to an integrated cell processing method, system and medium, a method for detecting cell activity, and a multi-channel control valve module. Background Art

[0002] Currently, cell therapy is a key development area at the forefront of international medicine. In the process of cell therapy, cell culture or cell subculture plays a key role in the process.

[0003] The existing cell culture or cell subculture process mainly relies on manual operation in a sterile environment. In this process, there are subjective factors and the influence of sterile sites, which leads to a high error rate and low accuracy rate in the whole process. In addition, the operation threshold for operators is high, the manual cycle is long, and the cell fluid needs to be transferred between steps. There are many steps and high labor costs. Therefore, there is an urgent need for an integrated cell processing technology to solve the above problems. Summary of the invention

[0004] The embodiments of the present invention provide an integrated cell processing method, device and medium to solve the problems in the prior art that the cell subculture process mainly relies on manual operation, which is affected by human subjective factors and the operation site, resulting in a high error rate, as well as a long manual operation cycle and high cost.

[0005] An integrated cell processing method comprising:

[0006] Connecting the cell processing integrated module via a pipeline through a multi-channel control valve module, and obtaining the sample liquid to be processed through the multi-channel control valve module;

[0007] The obtained sample liquid to be processed is separated and cleaned by the cell processing integrated module to obtain the target cell liquid.

[0008] A method for detecting cell activity, wherein the cell processing integrated module comprises a shaking platform, a culture container detachably connected to the shaking platform, a rotation controller fixedly connected to the shaking platform, and a multi-channel control valve module connected to the culture container via a pipeline, wherein the shaking platform, the culture container, the rotation controller and the multi-channel control valve module are all aseptically connected together to form a closed system, and the method comprises:

[0009] Controlling the switching of the multi-channel control valve module, dispensing the target cell solution in the integrated cell processing module for executing the above-mentioned integrated cell processing method into at least one culture container by driving the pump, and injecting complete culture medium into each of the culture containers;

[0010] Taking real-time images of the subculture of each culture container; and / or obtaining parameter indicators of the target cells in the culture container through at least one parameter sensor;

[0011] Cell analysis is performed based on all of the subculture images and / or all of the parameter indicators to obtain activity detection results corresponding to the target cells.

[0012] A multi-channel control valve module, applied to a cell processing integrated module, comprises a valve body, a valve core and a driving device;

[0013] The valve body is provided with a valve cavity for reciprocating movement of the valve core; the valve core is movably nested in the valve cavity;

[0014] At least one pipeline channel is provided on the valve body, wherein the pipeline channel is used to connect the pipeline channel in the valve core and the external peripheral pipeline;

[0015] By moving the valve core, the tube core channel is selectively matched and communicated with any of the pipeline channels;

[0016] The driving device is connected to the valve core and is used for driving the valve core to move.

[0017] A cell processing system comprises a controller, a container to be processed, a driving pump, a centrifugal container, a culture container and a multi-channel control valve module. The controller is used to execute the integrated cell processing method or the method for detecting cell activity.

[0018] A computer-readable storage medium stores a computer program, and when the computer program is executed by a processor, the integrated cell processing method or the method for detecting cell activity is implemented.

[0019] The above-mentioned integrated cell processing method, device and medium, in the integrated cell processing method of the present invention, the cell processing integrated module is connected to the multi-channel control valve module through a pipeline, and the sample liquid to be processed is obtained through the multi-channel control valve module; the obtained sample liquid to be processed is separated and cleaned by the cell processing integrated module to obtain the target cell liquid. The present invention realizes the automatic transfer of the sample liquid to be processed through the multi-channel control valve module and the cell processing integrated module, reduces the cell processing cycle, improves the cell processing efficiency, avoids the transfer of the operation site, reduces the influence of external factors, ensures that the whole process is in a sterile environment, and thus improves the culture efficiency and qualified rate. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for use in the description of the embodiments of the present invention will be briefly introduced below. Obviously, the accompanying drawings in the following description are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative labor.

[0021] Figure 1 is a flow chart of an integrated method for cell processing in one embodiment of the present invention;

[0022] Figure 2 is a flow chart of a method for detecting cell activity in one embodiment of the present invention;

[0023] Figure 3 is a cross-sectional view of a multi-channel control valve module in one embodiment of the present invention.

[0024] Description of reference numerals:

[0025] 1. Valve body; 2. Valve core; 3. Valve chamber; 4. Pipe core channel; 5. Pipe channel; 6. Switch assembly; 7. Air filter; 8. Through hole. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] In one embodiment, if Figure 1 As shown, a cell processing integrated method is provided, comprising the following steps:

[0028] S10: connecting the cell processing integrated module via a pipeline through the multi-channel control valve module, and obtaining the sample liquid to be processed through the multi-channel control valve module.

[0029] Understandably, the multi-channel control valve module refers to a control valve used to connect different channels between external channels and the cell integration module. The cell processing integration module refers to an integrated device for cell processing such as cell washing, cell culture, and cell packaging. The sample liquid to be processed refers to the cell liquid that needs to be processed, and the cell liquid includes cells for subculture (for example, stem cells, etc.). The pipeline refers to a disposable consumable tube used to connect different devices or modules.

[0030] Specifically, the multi-channel control valve module and the cell processing integrated module are connected through pipelines, that is, the channels of the multi-channel control valve module are connected to different devices in the cell processing integrated module through pipelines, so that different devices can be connected by switching the multi-channel control valve module. Then, the multi-channel control valve module is controlled to switch the connecting pipeline, so that the container containing the sample liquid to be processed is connected to the cell processing integrated module. The multi-channel control valve module can be compatible with the connection between various pipelines, such as compatible with the connection of culture vessels, culture bottles, incubators, etc. Then, the driving pump in the cell processing integrated module rotates, and the sample liquid to be processed is input from the container through the multi-channel control valve module into the cell processing integrated module.

[0031] S20: separating and washing the obtained sample solution to be processed by the integrated cell processing module to obtain target cell solution.

[0032] Understandably, the target cell fluid refers to the cell fluid required after cell washing, and the target cell fluid can be stem cell fluid for culture, cell fluid for subsequent cell treatment, or cell fluid for cell passage.

[0033] Specifically, the cell processing integrated module is controlled to separate and clean the sample liquid to be processed, that is, by switching the multi-channel control valve module, the container containing the cleaning liquid is connected to the cell processing integrated module, and the driving pump is controlled to rotate to add the cleaning liquid to the cell processing integrated module through the multi-channel control valve module. The cleaning liquid can also be added to the cell processing integrated module without passing through the multi-channel control valve module, that is, by switching the valve in the cell integration, the pipeline path is connected to the container containing the cleaning liquid, and the cleaning liquid is added to the centrifugal container in the cell processing integrated module by driving the rotation of the pump. Then, the mixed cleaning liquid and the sample liquid to be processed are centrifuged at high speed by driving the cell processing integrated module, so that the mixed solution is stratified in the cell processing integrated module, and the waste liquid (other cells or impurities other than the target cell liquid) after stratification in the cell processing integrated module is discharged to the waste liquid container through the waste discharge pipeline in the cell processing integrated module, so that the target cell liquid is obtained.

[0034] In the integrated cell processing method of the present invention, the multi-channel control valve module is connected to the integrated cell processing module through a pipeline, and the sample liquid to be processed is obtained through the multi-channel control valve module; the obtained sample liquid to be processed is separated and cleaned by the integrated cell processing module to obtain the target cell liquid. The present invention realizes the automatic transfer of the sample liquid to be processed through the multi-channel control valve module and the integrated cell processing module, reduces the cell processing cycle, improves the cell processing efficiency, avoids the transfer of the operation site, reduces the influence of external factors, ensures that the whole process is in a sterile environment, and thus improves the culture efficiency and qualified rate.

[0035] In one embodiment, after step S20, that is, after the cell processing integrated module separates and cleans the sample liquid to be processed to obtain the target cell liquid, the following steps are included:

[0036] The target cell fluid is output to the culture container through the multi-channel control valve module.

[0037] Understandably, the culture container refers to a container for culturing cells, such as a culture bottle, a culture tank, a culture dish, and specifically a T172 model culture bottle, etc. The culture container in this embodiment is provided with an air filter that interacts with the external air to adjust the pressure in the culture container. The air filter can be provided on the bottle cap of the culture container, and the bottle cap of the culture container is provided with a conduit connecting the inner and outer pipelines of the culture container.

[0038] Specifically, after obtaining the target cell fluid, the multi-channel control valve module is controlled to switch channels so that the centrifugal container and the culture container in the cell processing integrated module are connected. Then, the drive pump is controlled to rotate to output the target cell fluid in the centrifugal container through the multi-channel control valve module to the culture container for cell culture.

[0039] In one embodiment, the quantitative pump dispensing parameters corresponding to the target cell fluid are obtained, and the multi-channel control valve module is controlled to switch channels, so that the centrifugal container and the first culture container are connected, and then the driving pump is driven to rotate by controlling the quantitative pump dispensing parameters to output the quantitative target cell fluid from the centrifugal container to the first culture container, until all the target cell fluids are quantitatively dispensed into a preset number of culture containers. Wherein, when it is necessary to dispense different capacities to one or more culture containers, after the quantitative dispensing of the first quantitative pump dispensing parameters is completed, the remaining target cell fluid in the centrifugal container can be output to the culture container through the new pump dispensing parameters.

[0040] In another embodiment, after determining that the centrifugal container has been connected to the first culture container, the cell fluid flow rate of the target cell fluid flowing out of the centrifugal container is detected by a channel sensor set in the multi-channel control valve module. When it is detected that the cell fluid flow rate reaches a preset capacity, that is, when the channel sensor detects that the flow rate of the target cell fluid reaches the quantitative packaging, the multi-channel control valve module is controlled to switch, and the centrifugal container is connected to the next culture container until the target cell fluid is fully packaged into a preset number of culture containers. Wherein, when there are different quantitative packaging requirements, each quantitative packaging requirement is completed in sequence.

[0041] In another embodiment, after determining that the centrifugal container has been connected to the first culture container, the volume of the solution in the first culture container is monitored, that is, the weight of the culture container is monitored by a weighing sensor set on the shaking platform, or a sensor set on one side detects the height of the solution in the culture container, so that when the volume of the solution reaches a preset capacity, that is, when the volume of the target cell solution in the culture container is detected to reach the quantitative packaging, the centrifugal container is switched to be connected to the next culture container until the target cell solution is fully packaged into a preset number of culture containers. Among them, the packaging methods in the above embodiments can be used simultaneously to make the quantitative packaging more accurate.

[0042] That is, in this embodiment, the target cell solution is output to the culture container through the multi-channel control valve module, so as to realize the subpackaging of the target cell solution. The target cell solution is subpacked by one or more methods including quantitative pump subpackaging parameters, channel sensors and monitoring solution volume, so as to realize the accurate subpackaging of the target cell solution and ensure that the target cell solution in the culture container meets the quantitative requirements.

[0043] In one embodiment, the step S10, that is, connecting the cell processing integrated module through a pipeline through a multi-channel control valve module, and obtaining the sample liquid to be processed through the multi-channel control valve module, includes:

[0044] S101, injecting a preset solution into a container to be processed containing an initial cell solution, and using a shaking platform to flatten the bottom of the initial cell solution in the container to be processed, and making the target cells in the initial cell solution in a suspended state.

[0045] S102, controlling the multi-channel control valve module to switch to the first channel, and transferring the sample liquid to be processed in the container to be processed to the centrifugal container of the cell processing integrated module.

[0046] Understandably, the preset solution refers to one or more solutions set in advance, such as a buffer solution, a cleaning solution, etc. The centrifugal container refers to a container used to separate impurities, including but not limited to a centrifuge cup, a piston centrifuge cylinder, a centrifuge tank or a centrifuge bottle, etc. The container to be processed refers to a container used to hold the initial cell fluid, such as a fluid bag, a culture bottle, a culture dish, etc.

[0047] Specifically, the switching of the multi-channel control valve module is controlled so that the container containing the preset solution is connected to the container to be processed. The driving pump in the cell processing integrated module is controlled to rotate to inject the preset solution into the container to be processed containing the initial cell solution. Then, the preset solution and the initial cell solution are evenly mixed by evenly shaking the shaking platform detachably connected to the container to be processed, so that the target cells in the initial cell solution can better digest the preset container, and after the shaking of the shaking platform is finished, the initial cell solution in the container to be processed is flattened at the bottom, and the target cells in the initial cell solution are suspended, so that the target cells can continue to grow, and the number of cells is also increased.

[0048] In another embodiment, the cell is obtained by the adherent growth cell passaging method, that is, the initial cell fluid in the container to be processed is flattened to the bottom, and the enzymatic digestion method is used for passaging. A 0.25% trypsin solution is commonly used. The main steps are as follows: the culture fluid in the container to be processed is pumped into a waste liquid container, and the waste liquid can be a supernatant. The channel is switched by a multi-channel control valve module, and 0.25% trypsin solution is added (the amount added is determined by the digestion solution covering the entire bottom of the bottle), and the cell digestion is dynamically monitored for 2 to 10 minutes (the cell dynamics in the container to be processed can be collected by a camera), and an equal volume of culture fluid is added through the multi-channel control valve module to neutralize the digestion effect of the trypsin solution, and the target cells in the initial cell fluid are in a suspended state.

[0049] Furthermore, the multi-channel control valve module is controlled again to switch channels so that the cell processing integrated module is connected to the processing container through the first channel. Then, the driving pump is controlled to rotate to transfer the sample liquid to be processed in the processing container to the centrifugal container of the cell processing integrated module.

[0050] That is, in this embodiment, by using the preset solution and the shaking platform, the initial cell solution is flattened on the bottom of the culture container, and the target cells are suspended in the cell solution, thereby avoiding the target cells in the cell solution from adhering to the wall. The transfer of the sample solution to be processed is achieved through the first channel.

[0051] In one embodiment, after step S102, that is, after the sample liquid to be processed in the container to be processed is transferred to the centrifugal container of the integrated cell processing module, the following steps are included:

[0052] S103, purifying the sample liquid to be processed by high-speed centrifugation of the centrifugal container to obtain target cell liquid.

[0053] S104, controlling the switching of the multi-channel control valve module, dispensing the target cell solution into at least one culture container by driving a pump, and injecting complete culture medium into each culture container.

[0054] It can be understood that complete culture medium refers to a culture medium that is supplemented with a series of necessary nutrients and growth factors on the basis of basal culture medium to meet the various nutrients and growth factor requirements required for the growth and reproduction of specific cells.

[0055] Specifically, after the sample liquid to be processed in the container to be processed is transferred to the centrifugal container of the integrated cell processing module, the switching of the multi-channel control valve module is controlled so that the liquid bag containing the cleaning liquid is connected to the centrifugal container, and the cleaning liquid is injected into the centrifugal container by controlling the rotation of the driving pump. The purification operation is to separate the mixed liquid according to the size and density of cells of different masses through high-speed centrifugation of the centrifugal container. The centrifugal speed of the high-speed centrifugation can be greater than or equal to 500rpm. The centrifugal container is controlled to rotate at a high speed centrifugation to centrifuge and purify the sample liquid to be processed, that is, the liquid in the container is stratified during the centrifugal rotation of the centrifugal container, so as to obtain a cell impurity solution layer and a target cell solution layer during the centrifugation process. Then, the waste liquid is discharged through the input / output channel in the centrifugal container, and the cell impurities are discharged to obtain a liquid containing target cells. Furthermore, the multi-channel control valve module is continuously controlled to switch channels so that the centrifugal container and the first culture container are connected, and then the driving pump is controlled to rotate, so that a certain amount of target cell fluid is extracted from the centrifugal container and transferred to the first culture container, and then the culture container connected to the centrifugal capacity is switched through the multi-channel control valve module, and the driving pump is controlled to rotate, and a certain amount of target cell fluid is extracted and transferred to the second culture container, until all target cell fluids are transferred to a preset number of culture containers. Finally, the container containing the complete culture medium is connected to the multi-channel control valve module, and then the driving pump is controlled to rotate, and a preset volume of complete culture medium is injected from the container containing the complete culture medium into the first culture container, and the multi-channel control valve module is controlled to switch channels, and the preset volume of complete culture medium is injected into the next culture container, until all culture containers are injected with the preset volume of complete culture medium, and then the cells are cultured in the culture container according to the preset culture time.

[0056] That is, in this embodiment, by cleaning the sample solution to be processed, impurities are removed, thereby obtaining the target cell solution. By controlling the switching of the multi-channel control valve module, the centrifugal container is connected to each culture container in turn, thereby transferring the target cell solution, thereby culturing the cells in the target cell solution.

[0057] In one embodiment, in step S101, a preset solution is injected into a container to be processed containing an initial cell solution, and the initial cell solution in the container to be processed is flattened at the bottom by a shaking platform, and the target cells in the initial cell solution are suspended, wherein the preset solution includes a target buffer, an aggregation solution, and a stop solution; including:

[0058] S1011, adding a target buffer solution into the container to be processed to wash the sample solution to be processed.

[0059] S1012, switching to the second channel through the multi-channel control valve module, so that the liquid bag containing the aggregated liquid is connected to the container to be processed through the second channel, and controlling the driving pump of the cell processing integrated module to inject the aggregated liquid in the liquid bag into the container to be processed through the second channel.

[0060] S1013, controlling a shaking platform detachably connected to the container to be processed to perform a mixing operation, so that the aggregated liquid injected into the container to be processed is evenly mixed with the initial cell liquid after the cleaning operation, and the initial cell liquid in the container to be processed flattens the bottom.

[0061] S1014, controlling the switching of the multi-channel control valve module, adding a stop solution into the container to be processed, and making the target cells in the initial cell solution in a suspended state.

[0062] Understandably, the preset solution includes a target buffer, an aggregation solution and a stop solution. The target buffer refers to a PBS (Phosphate Buffered Saline) solution that does not contain calcium and magnesium ions. The aggregation solution refers to a solution used to aggregate cells so that the cells adhere to the bottom of the container to be processed, for example, 0.25% trypsin. The stop solution refers to a solution that stops the cells from aggregating to the bottom of the container to be processed, for example, a serum-free trypsin stop solution.

[0063] Specifically, the switching of the multi-channel control valve module is controlled so that the processing container is connected to the container containing the target buffer solution, and the target buffer solution is transferred to the container to be processed by driving the pump to rotate, and the shaking platform detachably connected to the container to be processed is controlled to shake, so as to clean the initial cell fluid in the container to be processed, and extract the supernatant during the cleaning process. Among them, the initial cell fluid in the container to be processed can be cleaned multiple times. Then, the multi-channel control valve module is switched to the second channel, so that the liquid bag containing the aggregate liquid is connected to the container to be processed through the second channel. By controlling the rotation of the driving pump in the cell processing integrated module, the aggregate liquid in the liquid bag is injected into the container to be processed through the second channel. Furthermore, a shaking platform detachably connected to the container to be processed is controlled to perform a mixing operation, that is, through a rotation controller fixedly connected to the shaking platform, the shaking platform is controlled to move in a preset shaking manner. The preset shaking manner movement can be a left and right rotation in the horizontal direction, or an up and down shaking in the longitudinal direction, or a left and right rotation in the horizontal direction and an up and down shaking in the longitudinal direction at the same time, so that the aggregation liquid injected into the container to be processed and the initial cell liquid after the cleaning operation are evenly mixed, and after the shaking platform finishes the movement, the initial cell liquid in the container to be processed flattens the bottom, and the target cells adhere to the bottom of the container to be processed under the action of the aggregation liquid.

[0064] Next, the channel connected to the multi-channel control valve module is switched again, so that the liquid bag containing the stop solution is connected to the container to be processed, and the driving pump in the cell processing integrated module is controlled to rotate, and the stop solution in the liquid bag is injected into the container to be processed. At the same time, air is repeatedly blown into the bottom of the container to be processed by the driving pump, so that the target cells in the container to be processed are in a suspended state. Alternatively, the shaking platform is controlled by the rotating controller to shake, so that the target cells in the container to be processed are in a suspended state.

[0065] That is, in this embodiment, the multi-channel control valve module is switched to achieve the connection between the liquid bag containing the target buffer, the aggregation liquid and the stop liquid and the container to be processed, thereby achieving the injection of the target buffer, the aggregation liquid and the stop liquid into the container to be processed. The shaking platform is controlled to perform the mixing operation, thereby achieving the uniform mixing of the aggregation liquid and the initial cell liquid, thereby achieving the initial cell liquid to flatten the bottom, thereby achieving the target cells in a suspended state.

[0066] In one embodiment, the step S1011, i.e. controlling the shaking platform detachably connected to the container to be processed to perform a mixing operation, includes:

[0067] A rotation controller fixedly connected to the shaking platform is controlled to drive the shaking platform to reciprocate at a preset vertical angle and / or rotate back and forth at a preset horizontal angle to evenly shake the container to be processed installed on the shaking platform and the aggregated liquid and the initial cell liquid in the container to be processed.

[0068] Understandably, the rotation controller refers to a controller for controlling the movement of the shaking platform. The preset horizontal angle refers to the horizontal rotation angle set in advance, for example, 30 degrees, 45 degrees, etc. The preset vertical angle refers to up and down movement, for example, the up and down swing angle is 20 degrees, 30 degrees, etc.

[0069] Specifically, after the preset solution is injected into the container to be processed containing the initial cell fluid, the rotation controller fixedly connected to the shaking platform is controlled to start, and the rotation controller drives the shaking platform to reciprocate at a preset vertical angle, and / or drives the shaking platform to rotate back and forth at a preset horizontal angle, thereby evenly shaking the container to be processed installed on the shaking platform, and mixing the aggregated liquid and the initial cell fluid in the container to be processed evenly. The back-and-forth rotation at the horizontal angle can spread the preset solution in a direction away from the mouth of the container to be processed and in the rotation direction according to the centrifugal force, thereby further accelerating the paving efficiency.

[0070] That is, in this embodiment, by controlling the rotation controller to drive the shaking platform to move, uniform shaking of the container to be processed is achieved, thereby achieving uniform mixing of the aggregation liquid and the initial cell liquid, so that the target cells can quickly digest the aggregation liquid.

[0071] In one embodiment, the step S103, i.e., purifying the sample solution to be processed by high-speed centrifugation of the centrifugal container to obtain the target cell solution, includes:

[0072] S1031, controlling a shaking platform detachably connected to the container to be processed to tilt toward the container mouth end, so that all the sample liquid to be processed in the container to be processed is collected at the outlet of the container to be processed.

[0073] S1032, controlling the multi-channel control valve module to connect the container to be processed and the centrifugal container, and controlling the driving pump to drive the sample liquid to be processed in the container to be processed to be transferred to the centrifugal container.

[0074] S1033, adding a cleaning solution into the centrifugal container, driving the centrifugal container to perform centrifugal rotation and performing a cleaning operation to clean the sample solution to be processed in the centrifugal container.

[0075] S1034, adding a resuspension liquid to the centrifugal container after the washing operation is completed, so that the target cells are resuspended in the mixed liquid in the centrifugal container to obtain a target cell liquid.

[0076] It can be understood that the mixed liquid refers to a solution obtained by mixing the resuspension liquid and the sample liquid to be processed.

[0077] Specifically, after suspending the cells, the shaking platform detachably connected to the container to be processed is controlled to tilt toward the container mouth end, that is, by raising the other end of the shaking platform corresponding to the container mouth of the container to be processed, all the sample liquid to be processed in the container to be processed is collected at the outlet of the container to be processed. Then, the connecting channel of the multi-channel control valve module is switched so that the container to be processed and the centrifugal container are connected. The driving pump is started to transfer the sample liquid to be processed collected at the outlet of the container to be processed to the centrifugal container through the multi-channel control valve module. Then, the multi-channel control valve module can be further switched so that the liquid bag containing the cleaning liquid is connected to the centrifugal container, and the driving pump is controlled to add the cleaning liquid to the centrifugal container. The centrifugal container is controlled to rotate centrifugally at a preset speed, so that the sample liquid to be processed in the centrifugal container is cleaned by the cleaning liquid. After the cleaning is completed, the waste liquid separated in the centrifugal container is discharged into the waste liquid container through the waste discharge port of the centrifugal container. Furthermore, the multi-channel control valve module is controlled to switch the interface connected to the centrifugal container, so that the container containing the resuspension is connected to the centrifugal container, and the driving pump is controlled to add the resuspension in the connected container into the centrifugal container, and the resuspension and the liquid in the centrifugal container are evenly mixed through the low-speed rotation of the centrifugal container, so that the target cells are re-suspended in the mixed liquid in the centrifugal container, and the obtained mixed liquid is determined as the target cell liquid.

[0078] That is, in this embodiment, by tilting the shaking platform toward the mouth of the container, the sample liquid to be processed is collected, and the transfer of the sample liquid to be processed is facilitated. The switching of the multi-channel control valve module is controlled to ensure the sterile environment of the channel, and the automatic switching of the channel is realized, which is convenient for the transfer of the sample liquid to be processed, thereby realizing the cleaning of the sample liquid to be processed, and then realizing the re-suspension of the cells and the acquisition of the target cell liquid.

[0079] In one embodiment, the step S104, i.e., controlling the switching of the multi-channel control valve module, and dispensing the target cell solution into at least one culture container by driving a pump, includes:

[0080] The quantitative pump dispensing parameters corresponding to the target cell fluid are obtained, and the driving pump is controlled to rotate according to the quantitative pump dispensing parameters to quantitatively dispense the target cell fluid into a preset number of the culture containers.

[0081] and / or

[0082] After determining that the centrifugal container is connected to the first culture container, the cell fluid flow of the target cell fluid flowing out of the centrifugal container is detected by a channel sensor. When it is detected that the cell fluid flow reaches a preset capacity, the centrifugal container is switched to connect to the next culture container until all the target cell fluid is loaded into a preset number of culture containers.

[0083] and / or

[0084] After determining that the centrifugal container is connected to the first culture container, the volume of the solution in the first culture container is monitored, and when the volume of the solution reaches a preset capacity, the centrifugal container is switched to connect to the next culture container until all the target cell solution is filled into a preset number of culture containers.

[0085] Understandably, the quantitative pump dispensing parameters refer to the speed and driving time of the driving pump. The preset quantity can be one or more. Quantitative dispensing refers to dispensing the cell fluid into each culture container according to the preset capacity requirements. Channel sensor is a sensor used to measure flow rate or flow. The cell fluid flow rate refers to the liquid volume of the target cell fluid flowing through the sensor. The preset capacity refers to the volume of the target cell fluid dispensed into each culture container set in advance. The solution volume refers to the volume of the target cell fluid in the monitored culture container.

[0086] Specifically, the quantitative pump dispensing parameters corresponding to the target cell fluid are obtained, and then the driving pump is controlled to rotate by the quantitative pump dispensing parameters, that is, the driving pump is controlled to rotate by the driving pump rotation speed and rotation time in the quantitative pump dispensing parameters, and a quantitative target cell fluid is extracted from the centrifugal container and dispensed into the first culture container. Then, the culture container connected to the centrifugal container is switched to continue the quantitative dispensing until all the target cell fluid in the centrifugal container is dispensed into a preset number of culture containers.

[0087] In another embodiment, after determining that the centrifugal container has been connected to the first culture container, the cell fluid flow rate of the target cell fluid flowing out of the centrifugal container is detected by a channel sensor, that is, the flow rate of the target cell fluid flowing through the multi-channel control valve module is detected by a channel sensor set in the multi-channel control valve module, thereby obtaining the cell fluid flow rate. When it is detected that the cell fluid flow rate reaches the preset capacity for quantitative packaging, the multi-channel control valve module is controlled to switch the culture container connected to the centrifugal container so that the centrifugal container is connected to the next culture container, and the cell fluid flow rate is continued to be detected by the channel sensor. When it is detected that the cell fluid flow rate reaches the preset capacity for quantitative packaging, the culture container connected to the centrifugal container is switched again until the target cell fluid is fully packaged into the preset number of culture containers.

[0088] In another embodiment, after determining that the centrifugal container has been connected to the first culture container, the volume of the solution in the first culture container is monitored, that is, the volume of the solution is detected by a weighing sensor disposed at the bottom of the culture container, or by a height sensor disposed on one side of the transparent portion of the culture container, the liquid level of the target cell solution in the culture container is monitored, and the volume of the solution is calculated by the liquid level. When it is detected that the volume of the solution reaches a preset capacity, the multi-channel control valve module is controlled to switch the culture container connected to the centrifugal container, so that the centrifugal container is connected to the next culture container, and the next culture container is continuously monitored until the target cell solution in the centrifugal container is fully loaded into a preset number of culture containers. The weight of the centrifugal container is weighed to determine that the target cell solution has been fully loaded.

[0089] That is, in this embodiment, by means of quantitative pump filling parameters, and / or by means of channel sensors, and / or by means of monitoring the culture container, it is possible to accurately fill the target cell fluid into the culture container, thereby improving the filling accuracy of the target cell fluid, thereby ensuring that the cells can be cultured normally in the culture bottle and avoiding the cells from growing into a dense monolayer in the culture bottle (i.e., the suspended cells will fill the culture fluid in the culture container).

[0090] In one embodiment, after step S104, that is, controlling the switching of the multi-channel control valve module, dispensing the target cell solution into at least one culture container by driving a pump, and injecting complete culture medium into each culture container, the following steps are included:

[0091] S105, switching the interfaces connected to the respective culture containers through the multi-channel control valve module for the cultured cell fluids in all the culture containers, and gathering them into one interface to input into the centrifugal container in the integrated cell processing module.

[0092] S106, washing the cultured cell fluid in the centrifugal container for a second time, and then dispensing the cell fluid into a plurality of cell factory modules.

[0093] S107, performing subculture on the target cells in the cultured cell fluid in the cell factory module, and monitoring the cell status of the target cells during the subculture.

[0094] S108, when the cell state reaches a preset state, transferring the post-culture cell fluid in the cell factory module to a target device, and washing and purifying the post-culture cell fluid to obtain a washed cell fluid.

[0095] S109, after transferring the washed cell fluid to the sub-packaging device, controlling the sub-packaging device to sub-pack the washed cell fluid into at least one sub-packaging container.

[0096] Understandably, the cell factory module refers to a single-layer or multi-layer culture vessel. The target device refers to a device used to clean and purify the cell fluid, such as a centrifuge cup. The heat sealing device refers to a device that can be disconnected after heating and closing the product packaging, such as a heat sealer, so that the product packaging is sealed. The filling container refers to a container for holding the cleaned cell fluid, such as a product bag. The freezing equipment refers to a device used to freeze and store cells for a long or short period of time. The preset state refers to a required state set in advance, such as some target cells no longer dividing, etc. The filling equipment refers to a device used to fill the solution.

[0097] Specifically, by switching the multi-channel control valve module, the centrifugal container is connected to each culture container in turn, that is, after the cultured cell fluid in the previous culture container is completely transferred to the centrifugal container, the interface of the multi-channel control valve module is switched, and the cultured cell fluid in the next culture container is transferred to the centrifugal container, until the cultured cell fluid in all culture containers is completely transferred to the centrifugal container through the multi-channel control valve module. Then, a cleaning solution is added to the centrifugal container to remove impurities in the cultured cell fluid, that is, the process of secondary cleaning, and the waste liquid after separation is discharged through the waste outlet. Then, the multi-channel control module is controlled to switch, and the cell fluid after the secondary cleaning is driven by a driving pump to transfer to multiple cell factory modules, or to a multi-layer cell factory module. Among them, the cell fluid after the secondary cleaning can be accurately divided into each cell factory module by driving the pump rotation parameters, and / or the channel sensor monitors the flow, and / or monitors the cell factory module.

[0098] Further, a culture fluid is added to the cell factory module, and the parameters of the cell factory module are adjusted, so that the target cells in the cultured cell fluid are subcultured in the cell factory module, and the cell state of the target cells in the subculture of the cell factory module is monitored by a monitoring module arranged above or on one side of the cell factory module, and the parameter index transmitted by the parameter sensor in the cell factory module and the image taken by the monitoring module are analyzed for activity, and the cell state of the target cells in the subculture is determined according to the obtained activity detection results. Then, when the cell state is monitored to reach a preset state, the culture of the target cells is stopped, and the multi-channel control valve module is controlled to switch the connection channel so that the cell factory module is connected to the target device, so that all the subcultured cell fluids in all the cell factory modules are transferred to the target device. Then, the subcultured cell fluid is cleaned and purified by the target device, that is, a cleaning fluid is added to the target device to clean and purify the cultured cell fluid, and the impurities in the cell fluid are separated and discharged to obtain the cleaned cell fluid.

[0099] Furthermore, the switching of the multi-channel control valve module is controlled so that the target device and the sub-packaging device are connected, and the driving pump is controlled to drive the cleaned cell fluid in the target device to be transferred to the sub-packaging device. Among them, the target device can be directly connected to the sub-packaging container, and the cell fluid can be quantitatively sub-packed. Next, the multi-channel control valve module is controlled to switch so that the sub-packaging device is connected to each sub-packaging container in turn, so that the cleaned cell fluid can be accurately sub-packed into each sub-packaging container. After the cleaned cell fluid of the preset sub-packaging capacity is sub-packed into the sub-packaging container, the container mouth of the sub-packaging container is heat-sealed and packaged by the heat-sealing device, so that the sub-packaging container is sealed. Then, each sub-packaging container that has been completed is heat-sealed and packaged in turn. Among them, the cell fluid flow entering the sub-packaging container can be monitored by a weighing sensor or a flow sensor to ensure the accuracy of the solution volume in the sub-packaging container. After all the cell fluids after cleaning are sub-packed, all sub-packaging containers are stored in a freezing device. Among them, after the sub-packaging container is packaged, it can be stored in a freezing device immediately.

[0100] That is, in this embodiment, the cell fluid after culture is cleaned to remove impurities, thereby realizing the subculture of the cell fluid after culture and monitoring the target cells during the subculture. The accuracy of the subpackaged cell fluid is ensured by the subpackaging of the subpackaging equipment and the sealing of the heat-sealing equipment, thereby realizing the heat-sealing packaging of the subpackaging container and the storage of the subpackaging container.

[0101] In one embodiment, if Figure 2 As shown, a method for detecting cell activity, the cell processing integrated module includes a shaking platform, a culture container detachably connected to the shaking platform, a rotation controller fixedly connected to the shaking platform, and a multi-channel control valve module connected to the culture container via a pipeline, the shaking platform, the culture container, the rotation controller and the multi-channel control valve module are all aseptically connected together to form a closed system, the method includes:

[0102] S1, controlling the switching of the multi-channel control valve module, dispensing the target cell fluid in the integrated cell processing module that executes the above-mentioned integrated cell processing method into at least one culture container by driving the pump, and injecting complete culture medium into each of the culture containers.

[0103] S2, photographing subculture images of each culture container in real time; and / or acquiring parameter indicators of target cells in the culture container through at least one parameter sensor.

[0104] S3, performing cell analysis based on all the subculture images and / or all the parameter indicators to obtain activity detection results corresponding to the target cells.

[0105] Understandably, the driving pump refers to a device used to transport cell fluid, such as a peristaltic pump. Complete culture medium refers to a culture medium that is added with a series of necessary nutrients and growth factors on the basis of the basal culture medium to meet the various nutrients and growth factor requirements required for the growth and reproduction of specific cells. The subculture image refers to the image of the target cells during the culture process. Parameter indicators refer to various quantitative indicators used to evaluate cell growth, morphology, function and quantity. The preset duration refers to a period of time set in advance, which can be ten minutes, twenty minutes, etc. The activity test results are used to characterize the survival, growth, proliferation, metabolism and functional status under the culture environment conditions.

[0106] The integrated cell processing module includes a shaking platform, a culture container detachably connected to the shaking platform, a rotation controller fixedly connected to the shaking platform, and a multi-channel control valve module connected to the culture container via a pipeline. The shaking platform, the culture container, the rotation controller and the multi-channel control valve module are all aseptically connected together to form a closed system.

[0107] Specifically, the multi-channel control valve module is controlled to switch the channels so that the centrifugal container and the culture container containing the target cell solution in the cell processing integrated module are connected. Then, by driving the driving pump in the cell processing integrated module, the target cell solution in the centrifugal container is divided into one or more culture containers for culture, and the channel connected to the culture container is switched to inject the complete culture medium into each culture container in turn. Further, the subculture images of each culture container are photographed in real time, that is, the culture container is continuously photographed by a shooting device arranged above the transparent part of the culture container, so as to obtain at least one subculture image corresponding to each culture container. And / or the parameter index of the cell used for subculture in the culture container is obtained by at least one parameter sensor. Among them, the parameter index of the cell can be obtained in real time, and the parameter index output by the parameter sensor can also be obtained when the shooting device shoots the image. A continuous subculture image of a preset duration is selected from all subculture images, and / or the parameter index of the corresponding time period is selected from all parameter indicators. Then, all parameter indicators and / or all subculture images corresponding to the same preset time length are subjected to cell analysis, that is, the target cells are detected to see whether they are dividing normally, or whether the position of the target cells has changed, or they are identified by the characteristics of division and position change, thereby obtaining activity detection results. Then, when the activity detection results indicate that the cells are dividing normally or the position of the cells has changed, it is determined that the target cells are growing normally within the preset time length. When the activity detection results indicate that the cells are not dividing normally or the position of the cells has not changed, it is determined that the target cells have begun to apoptosis within the preset time length.

[0108] In another embodiment, the lower part corresponding to the upper transparent part of the culture container is also set as a transparent part, that is, the culture container in the present application is not a fully transparent module, but a partially transparent module. A light source is set under the lower transparent part, and through the illumination of the light source, the upper shooting device corresponding to its position is used to collect images of the target cells in the culture container, so as to obtain at least one subculture image corresponding to each culture container.

[0109] In another embodiment, the continuous images captured in real time by the shooting device and the parameter indicators transmitted in real time by the parameter sensor are analyzed to determine the survival rate of the target cells by analyzing the growth of the proportion of cells in the shooting area within a unit time period, or to analyze the growth curve of the target cells or detect the survival rate of the target cells to determine the growth status of the target cells, thereby obtaining the activity detection result of the target cells.

[0110] That is, in this embodiment, by transferring the target cell solution in the integrated cell processing module to the culture container for culture, the target cells in the culture process are monitored and the parameter indicators of the target cells are obtained. The cell analysis is performed in combination with the parameter indicators and the subculture images to determine the activity detection results.

[0111] In one embodiment, if Figure 3 As shown, a multi-channel control valve module is applied to a cell processing integrated module, including a valve body, a valve core and a driving device;

[0112] The valve body is provided with a valve cavity for reciprocating movement of the valve core; the valve core is movably nested in the valve cavity;

[0113] At least one pipeline channel is provided on the valve body, wherein the pipeline channel is used to connect the pipeline channel in the valve core and the external peripheral pipeline;

[0114] By moving the valve core, the tube core channel is selectively matched and communicated with any of the pipeline channels;

[0115] The driving device is connected to the valve core and is used for driving the valve core to move.

[0116] It can be understood that the multi-channel control valve module includes a valve body 1, a valve core 2 and a drive device (in Figure 3(not shown). The valve body 1 is provided with at least one pipeline channel 5, which is used to connect the pipe core channel 4 in the valve core 2 and the external peripheral pipeline. The valve body 1 is provided with a valve cavity 3 for the reciprocating movement of the valve core 2. One end of the valve cavity 3 is closed, and the closed end is provided with a through hole 8 for balancing the pressure in the valve cavity. The through hole 8 is connected to an air filter 7 to filter the air entering the valve cavity 3. The other end of the valve cavity 3 is open, so that the valve core 2 can reciprocate. The valve core 2 is movably nested in the valve cavity 3 of the valve body 1, and is used to connect different pipeline channels 5 set on the valve body 1, that is, by controlling the valve core 2 by a driving device, the pipe core channel 4 in the valve core 2 can connect different pipeline channels 5 on the valve body 1. Among them, the multi-channel control valve module can achieve an environment with a sterile seal, and a bellows or a piston is provided between the valve core 2 and the valve body 1, so that the interior of the multi-channel control valve module is in a sterile environment.

[0117] In one embodiment, all the pipeline channels 5 are arranged on the same side of the valve body 1. Moreover, all the pipeline channels 4 may be spaced at a fixed distance or may not be spaced at a fixed distance. The driving device controls the valve core 2 to move linearly in the valve cavity 3, so that the pipeline core channels 4 are connected to different pipeline channels 5.

[0118] In another embodiment, all the pipeline channels 5 are arranged on different sides of the valve body 1. All pipeline channels 5 may be spaced at a fixed distance or may not be spaced at a fixed distance. The driving device controls the valve core 2 to rotate and move in the valve cavity 3, so that the pipeline channel 4 is connected to different pipeline channels 5.

[0119] In one embodiment, the pipeline channel 5 is provided with a switch component 6 for controlling the opening and closing of the channel.

[0120] It can be understood that the switch assembly 6 is used to control the opening and closing of the pipeline channel 5. When the positions of the core channel 4 and the pipeline channel 5 are correspondingly connected, the switch assembly 6 is controlled to open the pipeline channel 5. When the position of the core channel 4 changes relative to the position of the pipeline channel 5, the switch assembly 6 is controlled to close the pipeline channel. In another embodiment, the switch assembly 6 can be a one-way valve or a two-way valve. And the sensor is used to detect whether the positions of the core channel 4 and the pipeline channel 5 are correspondingly connected.

[0121] It should be understood that the order of execution of the steps in the above embodiment does not necessarily mean the order of execution. The execution order of each process should be determined by its function and internal logic, and should not constitute any limitation on the implementation process of the embodiment of the present invention.

[0122] A cell processing system comprises a controller, a container to be processed, a driving pump, a centrifugal container, a culture container and a multi-channel control valve module. The controller is used to execute an integrated cell processing method or the method for detecting cell activity.

[0123] For the specific definition of the cell processing integrated module, controller and its various units and modules, please refer to the definition of the cell processing integrated method above, which will not be repeated here. Each module in the above-mentioned controller can be implemented in whole or in part by software, hardware and a combination thereof. Understandably, the controller includes a processor, a memory, a network interface and a database connected by a device bus. Each module of the controller can be embedded in or independent of the processor in hardware form, or can be stored in the memory in software form, so that the processor can call and execute the operations corresponding to the above modules. Among them, the processor is used to provide computing and control capabilities. The memory includes a non-volatile storage medium and an internal memory. The non-volatile storage medium stores an operating device, a computer program and a database. The internal memory provides an environment for the operation of the operating device and the computer program in the non-volatile storage medium. The database is used to store the data used by the cell processing integrated method in the above-mentioned embodiment. The network interface is used to communicate with an external terminal through a network connection. When the computer program is executed by the processor, a cell processing integrated method or the method for detecting cell activity is implemented.

[0124] In one embodiment, a computer-readable storage medium is provided, wherein the computer-readable storage medium stores a computer program, and when the computer program is executed by a processor, the above-mentioned integrated cell processing method or the above-mentioned method for detecting cell activity is implemented.

[0125] Those skilled in the art can understand that all or part of the processes in the above-mentioned embodiment methods can be completed by instructing the relevant hardware through a computer program, and the computer program can be stored in a non-volatile computer-readable storage medium. When the computer program is executed, it can include the processes of the embodiments of the above-mentioned methods. Among them, any reference to memory, storage, database or other media used in the embodiments provided in this application can include non-volatile and / or volatile memory. Non-volatile memory can include read-only memory (ROM), programmable ROM (PROM), electrically programmable ROM (EPROM), electrically erasable programmable ROM (EEPROM) or flash memory. Volatile memory can include random access memory (RAM) or external cache memory. As an illustration and not limitation, RAM is available in many forms, such as static RAM (SRAM), dynamic RAM (DRAM), synchronous DRAM (SDRAM), double data rate SDRAM (DDRSDRAM), enhanced SDRAM (ESDRAM), synchronous link (Synchlink) DRAM (SLDRAM), memory bus (Rambus) direct RAM (RDRAM), direct memory bus dynamic RAM (DRDRAM), and memory bus dynamic RAM (RDRAM).

[0126] Those skilled in the art can clearly understand that for the convenience and simplicity of description, only the division of the above-mentioned functional units and modules is used as an example. In actual applications, the above-mentioned functions can be distributed and completed by different functional units and modules as needed, that is, the internal structure of the device can be divided into different functional units or modules to complete all or part of the functions described above.

[0127] The embodiments described above are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. Such modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention, and should all be included in the protection scope of the present invention.

Claims

1. A cell processing integrated method, characterized in that: include: Connecting the cell processing integrated module via a pipeline through a multi-channel control valve module, and obtaining the sample liquid to be processed through the multi-channel control valve module; The obtained sample liquid to be processed is separated and cleaned by the cell processing integrated module to obtain the target cell liquid.

2. The integrated cell treatment method according to claim 1, characterized in that: After the obtained sample solution to be processed is separated and cleaned by the cell processing integrated module to obtain the target cell solution, the method includes: The target cell fluid is output to the culture container through the multi-channel control valve module.

3. The integrated cell treatment method according to claim 1, characterized in that: The method of connecting the cell processing integrated module through a pipeline through the multi-channel control valve module and obtaining the sample liquid to be processed through the multi-channel control valve module includes: Injecting a preset solution into a container to be processed containing an initial cell solution, and using a shaking platform to flatten the bottom of the initial cell solution in the container to be processed, and making the target cells in the initial cell solution in a suspended state; The multi-channel control valve module is controlled to switch to the first channel, and the sample liquid to be processed in the container to be processed is transferred to the centrifugal container of the cell processing integrated module.

4. The integrated cell treatment method according to claim 3, characterized in that: After transferring the sample liquid to be processed in the container to be processed to the centrifugal container of the integrated cell processing module, the method includes: Purifying the sample solution to be processed by high-speed centrifugation of the centrifugal container to obtain target cell solution; The switching of the multi-channel control valve module is controlled, the target cell solution is dispensed into at least one culture container by driving a pump, and complete culture medium is injected into each culture container.

5. The cell subculture method according to claim 4, characterized in that: The method of purifying the sample solution to be processed by high-speed centrifugation of the centrifugal container to obtain the target cell solution includes: Controlling a shaking platform detachably connected to the container to be processed to tilt toward the container mouth end, so that all the sample liquid to be processed in the container to be processed is collected at the outlet of the container to be processed; Controlling the multi-channel control valve module to connect the container to be processed and the centrifugal container, and controlling the driving pump to drive the sample liquid to be processed in the container to be processed to be transferred to the centrifugal container; Adding a cleaning solution into the centrifugal container, and driving the centrifugal container to perform centrifugal rotation and a cleaning operation to clean the sample liquid to be processed in the centrifugal container; A resuspension liquid is added to the centrifugal container after the washing operation is completed, so that the target cells are resuspended in the mixed liquid in the centrifugal container to obtain a target cell liquid.

6. The integrated cell treatment method according to claim 3, characterized in that: The preset solution includes a target buffer, an aggregation solution and a stop solution; The method of injecting the preset solution into the container to be processed containing the initial cell solution, and making the initial cell solution in the container to be processed flat at the bottom by shaking the platform, and making the target cells in the initial cell solution in a suspended state, comprises: Adding a target buffer solution into the container to be processed to wash the sample solution to be processed; Switching to the second channel through the multi-channel control valve module enables the liquid bag containing the aggregate liquid to be connected to the container to be processed through the second channel, and controlling the driving pump of the cell processing integrated module to inject the aggregate liquid in the liquid bag into the container to be processed through the second channel; Controlling a shaking platform detachably connected to the container to be processed to perform a mixing operation, so that the aggregate solution injected into the container to be processed is evenly mixed with the initial cell solution after the cleaning operation, and the initial cell solution in the container to be processed is flattened at the bottom; The switching of the multi-channel control valve module is controlled, a stop solution is added to the container to be processed, and the target cells in the initial cell solution are placed in a suspended state.

7. The integrated cell treatment method according to claim 6, characterized in that: The controlling the shaking platform detachably connected to the container to be processed to perform a mixing operation comprises: A rotation controller fixedly connected to the shaking platform is controlled to drive the shaking platform to reciprocate at a preset vertical angle and / or rotate back and forth at a preset horizontal angle to evenly shake the container to be processed installed on the shaking platform and the aggregated liquid and the initial cell fluid in the container to be processed.

8. The integrated cell treatment method according to claim 4, characterized in that: The method of controlling the switching of the multi-channel control valve module and dispensing the target cell solution into at least one culture container by driving a pump comprises: Acquiring quantitative pump dispensing parameters corresponding to the target cell fluid, and controlling the driving pump to rotate according to the quantitative pump dispensing parameters to quantitatively dispense the target cell fluid into a preset number of culture containers; and / or After determining that the centrifugal container has been connected to the first culture container, detecting the cell fluid flow rate of the target cell fluid flowing out of the centrifugal container by a channel sensor, and when detecting that the cell fluid flow rate reaches a preset capacity, switching the centrifugal container to connect to the next culture container until all the target cell fluid is dispensed into a preset number of culture containers; and / or After determining that the centrifugal container is connected to the first culture container, the volume of the solution in the first culture container is monitored, and when the volume of the solution reaches a preset capacity, the centrifugal container is switched to connect to the next culture container until all the target cell solution is filled into a preset number of culture containers.

9. The integrated cell treatment method according to claim 4, characterized in that: The control of the switching of the multi-channel control valve module, the driving of the pump to dispense the target cell solution into at least one culture container, and the injection of complete culture medium into each culture container, comprises: The cultured cell fluids in all the culture containers are switched through the multi-channel control valve module to interfaces connected to the respective culture containers, and are collected into one interface and input into the centrifugal container in the integrated cell processing module; After the cultured cell fluid in the centrifugal container is washed twice, it is divided into a plurality of cell factory modules; Subculturing the target cells in the cultured cell fluid in the cell factory module, and monitoring the cell status of the target cells in the subculture; When the cell state reaches a preset state, transferring the passaged cell fluid in the cell factory module to a target device, and washing and purifying the passaged cell fluid to obtain a washed cell fluid; After the washed cell fluid is transferred to the sub-packaging device, the sub-packaging device is controlled to sub-pack the washed cell fluid into at least one sub-packaging container.

10. A method for detecting cell activity, characterized in that: The cell processing integrated module comprises a shaking platform, a culture container detachably connected to the shaking platform, a rotation controller fixedly connected to the shaking platform, and a multi-channel control valve module connected to the culture container via a pipeline. The shaking platform, the culture container, the rotation controller and the multi-channel control valve module are all aseptically connected together to form a closed system. The method comprises: Controlling the switching of the multi-channel control valve module, dispensing the target cell solution in the integrated cell processing module for performing the integrated cell processing method according to any one of claims 1 to 9 into at least one culture container by driving the pump, and injecting complete culture medium into each of the culture containers; Taking real-time images of the subculture of each culture container; and / or obtaining parameter indicators of the target cells in the culture container through at least one parameter sensor; Cell analysis is performed based on all of the subculture images and / or all of the parameter indicators to obtain activity detection results corresponding to the target cells.

11. A multi-channel control valve module, characterized in that: Applied to the integrated cell processing module, including valve body, valve core and drive device; The valve body is provided with a valve cavity for reciprocating movement of the valve core; the valve core is movably nested in the valve cavity; At least one pipeline channel is provided on the valve body, wherein the pipeline channel is used to connect the pipeline channel in the valve core and the external peripheral pipeline; By moving the valve core, the tube core channel is selectively matched and communicated with any of the pipeline channels; The driving device is connected to the valve core and is used for driving the valve core to move.

12. The multi-channel control valve module according to claim 11, characterized in that: The pipeline channel is provided with a switch component for controlling the opening and closing of the channel.

13. A cell processing system, characterized in that: It comprises a controller, a container to be processed, a driving pump, a centrifugal container, a culture container and a multi-channel control valve module, wherein the controller is used to execute the integrated cell processing method as described in any one of claims 1 to 9 or the method for detecting cell activity as described in claim 10.

14. A computer-readable storage medium storing a computer program, characterized in that: When the computer program is executed by a processor, the integrated cell processing method according to any one of claims 1 to 9 or the method for detecting cell activity according to claim 10 is implemented.

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