Freckle-removing and whitening essence and preparation method thereof

Abstract

The invention relates to the technical field of skin care products, and aims to provide freckle-removing and whitening essence and a preparation method thereof, and the freckle-removing and whitening essence comprises 0.5-3.5% of a whitening and freckle-removing agent, 5-10% of a humectant, 2-5% of a skin conditioner, 0.2-0.6% of a preservative, 0.1-0.3% of a thickener, 0.1-0.2% of a pH regulator, 0.02-0.05% of a chelating agent and the balance of water. The traditional Chinese medicine composition has a good tyrosinase activity inhibition effect, and can whiten and tender the skin and moisturize and nourish the skin after being used.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care products, and more specifically, to a freckle-removing and whitening essence and a preparation method thereof. Background Art

[0002] Cosmetics with whitening effects have always been popular among consumers and are also an important research direction for researchers in the cosmetics field. The degree of skin color is closely related to the melanin content in skin cells. The production of melanin is closely related to tyrosinase, which is specifically manifested in: tyrosinase catalyzes tyrosine to form dopa, and then continues to catalyze the formation of dopaquinone. Dopaquinone is then rearranged, polymerized, and combined with structural proteins in melanosomes to form melanin. Tyrosinase is an important rate-limiting enzyme in the production of melanin. Inhibiting tyrosinase activity can reduce the production of melanin, which is beneficial to whitening.

[0003] Therefore, it is necessary to develop a product with good tyrosinase activity inhibition to obtain better whitening effect. Summary of the invention

[0004] Based on the defects of the prior art, the object of the present invention is to provide a freckle removing and whitening essence.

[0005] The above-mentioned object of the present invention is achieved through the following technical scheme: a freckle removing and whitening essence, characterized in that it comprises the following components, by mass percentage: 0.5-3.5% of whitening and freckle removing agent, 5-10% of moisturizing agent, 2-5% of skin conditioning agent, 0.2-0.6% of preservative, 0.1-0.3% of thickener, 0.1-0.2% of pH adjusting agent, 0.02-0.05% of chelating agent, and the remainder of water.

[0006] Preferably, the whitening and anti-freckle agent comprises phenethylresorcinol, 4-butylresorcinol, vitamin C and glutathione encapsulated by cyclodextrin.

[0007] Preferably, the weight ratio of phenethylresorcinol, 4-butylresorcinol, vitamin C, and glutathione encapsulated by cyclodextrin is (3-5):(10-25):(5-10):(40-60).

[0008] Preferably, the method for preparing the cyclodextrin-encapsulated glutathione comprises the following steps: adding glutathione to cyclodextrin slurry, stirring, ultrasonicating, freeze-drying, crushing and sieving to obtain glutathione.

[0009] Preferably, the method for preparing the cyclodextrin-encapsulated glutathione comprises the following steps: adding glutathione to cyclodextrin slurry, stirring, ultrasonicating, freeze-drying, crushing and sieving to obtain glutathione.

[0010] Preferably, the moisturizing agent includes at least one of glycerin, butylene glycol and pentylene glycol.

[0011] Preferably, the skin conditioning agent comprises at least one of Poria cocos extract, Rehmannia glutinosa root extract and Panax ginseng root extract.

[0012] Preferably, the preparation method of the freckle removing and whitening essence comprises the following steps:

[0013] S1: Accurately weigh each component according to the formula, add water, humectant, thickener and chelating agent into the container, heat to 85±5℃, homogenize for 5 minutes and keep warm for 15 minutes;

[0014] S2: After cooling to 40-45°C, add pH regulator and stir evenly;

[0015] S3: Add the remaining components into the container and stir evenly to obtain the freckle removing and whitening essence.

[0016] The present invention has the following advantages over the prior art:

[0017] (1) Phenylethyl resorcinol and 4-butyl resorcinol can not only effectively inhibit the activity of tyrosinase and tyrosinase-related protein-1 (TRP-1), as well as inhibit the melanin of B16 melanoma cells synthesized by tyrosinase, but also regulate keratin renewal, strengthen the skin's keratin barrier, and enhance skin resistance; induce endothelial cell migration, capillary proliferation, promote fibroblast proliferation, effectively rebuild collagen fiber bundles, restore skin elasticity and toughness, and have anti-wrinkle and skin repair effects while whitening and lightening spots.

[0018] (2) Cyclodextrin encapsulation of glutathione overcomes the process impact caused by the strong hygroscopicity of glutathione, improves its aqueous solution stability, reduces the risk of oxidative decomposition of reduced glutathione, and ensures the stability of product efficacy; at the same time, vitamin C is added to prevent glutathione oxidation, which not only inhibits the activity of tyrosinase, but also reversibly hydrogenates or dehydrogenates, allowing the oxidized glutathione to be reduced to reduced glutathione.

[0019] (3) Glutathione was encapsulated in the cavity structure of cyclodextrin under ultrasound and freeze-drying process, reducing the effects of high temperature and pH on the content and stability of glutathione.

[0020] (4) The added moisturizer can help the skin heal, reduce the occurrence of UV-related dermatitis and restore the normal barrier function of the skin; it can promote the self-repair function of damaged cells. The moisturizer of the present application can work synergistically with the whitening and spot-lightening agent, and can moisturize the skin while whitening and spot-lightening, further improving the effect of the whitening and spot-lightening agent. DETAILED DESCRIPTION

[0021] The present invention is further described below in conjunction with embodiments, but the scope of protection claimed by the present invention is not limited to the scope described in the embodiments.

[0022] The formulas of Examples 1 to 5 of the present invention are as follows:

[0023] Table 1 Recipe

[0024]

[0025]

[0026] The preparation method of the freckle-removing and whitening essence of the above-mentioned embodiments 1 to 5 comprises the following steps:

[0027] S1: Accurately weigh each component according to the formula, add water, humectant, thickener and chelating agent into the container, heat to 85±5℃, homogenize for 5 minutes and keep warm for 15 minutes;

[0028] S2: After cooling to 40-45°C, add pH regulator and stir evenly;

[0029] S3: Add the remaining components into the container and stir evenly to obtain the freckle removing and whitening essence.

[0030] Comparative Example 1

[0031] The difference from Example 3 is that phenethyl resorcinol is omitted, and the contents of other components and the preparation method are the same as those in Example 3.

[0032] Comparative Example 2

[0033] The difference from Example 3 is that 4-butylresorcinol is omitted, and the contents of the remaining components and the preparation method are the same as those in Example 3.

[0034] Comparative Example 3

[0035] The difference from Example 3 is that vitamin C is omitted, and the contents of other components and the preparation method are the same as those in Example 3.

[0036] Comparative Example 4

[0037] The difference from Example 3 is that glutathione not encapsulated by cyclodextrin is used, and the contents and preparation methods of other components are the same as those of Example 3.

[0038] Comparative Example 5

[0039] Commercially available whitening and spot-lightening essence

[0040] Effect test

[0041] The products obtained in Examples 1 to 5 and the products in Comparative Examples 1 to 5 were tested for their effects:

[0042] The test conditions and methods are as follows:

[0043] 200 female volunteers aged between 35 and 45 with varying degrees of facial pigmentation were selected. All volunteers were in good health, had no history of skin diseases and allergies, and had non-sensitive skin.

[0044] All volunteers were randomly divided into 10 groups, and each group used the corresponding product.

[0045] All volunteers were able to use the products as specified, only used designated whitening and freckle-removing products during the trial, and cooperated with staff to complete the evaluation work as required. They had certain expressive abilities and could truly reflect their feelings after use.

[0046] How to use: Select a spot of about 5×5cm on the face of all volunteers with pigmentation. 2 An area of ​​size was used as the test area. After cleansing, the volunteers did not apply any makeup products, but applied the corresponding composition of each group on the test area, once in the morning and once in the evening, for 60 consecutive days.

[0047] The skin elasticity of the test areas of 10 groups of volunteers was tested using a skin elasticity meter and an intelligent water whitening detector. The changes in the blemish-lightening, whitening, glossy and delicate skin as well as the moisturizing of the skin in the test areas of the volunteers' faces before and after the test were observed. The test results are shown below.

[0048] Table 2 Usage effect data table

[0049] Test items Lightens spots Whitening Delicate gloss Moisturizing Example 1 37% improvement 40% improvement 45% improvement Improvement 38 Example 2 36% improvement 39% improvement 46% improvement 42% improvement Example 3 38% improvement 40% improvement 45% improvement 43% improvement Example 4 34% improvement 42% improvement 42% improvement 45% improvement Example 5 35% improvement 38% improvement 43% improvement 40% improvement Comparative Example 1 18% improvement 27% improvement 31% improvement 36% improvement Comparative Example 2 21% improvement 30% improvement 32% improvement 33% improvement Comparative Example 3 28% improvement 29% improvement 35% improvement 40% improvement Comparative Example 4 25% improvement 28% improvement 33% improvement 35% improvement Comparative Example 5 28% improvement 31% improvement 32% improvement 36% improvement

[0050] As can be seen from Table 2, the products of Examples 1 to 3 are compared with the products of Comparative Examples 1 to 5. When used, they not only have excellent light spot and whitening effects, can greatly improve the gloss and fineness of the skin, but also have significant moisturizing and nourishing effects, which can improve. Among them, when the essence of Comparative Examples 1 to 2 omits one of phenylethyl resorcinol and 4-butyl resorcinol, the essence of Comparative Examples 1 to 2 is significantly reduced in terms of the improvement of light spot, whitening and skin moisturizing effects compared with the composition of Examples 1 to 5. In Comparative Example 3, due to the omission of vitamin C, the essence inhibits the activity of tyrosinase, and in addition, it cannot reduce the oxidized glutathione to reduced glutathione again. In Comparative Example 4, since glutathione is not encapsulated, its aqueous solution stability is poor, there is a high risk of easy oxidation and decomposition, and the stability of the product efficacy is poor.

[0051] In summary, the essence of the present invention uses phenethyl resorcinol, 4-butyl resorcinol, vitamin C and cyclodextrin-encapsulated glutathione as whitening and spot-lightening agents. Among them, phenethyl resorcinol has excellent skin whitening effect, is a highly active tyrosinase inhibitor, can effectively inhibit the activity of B16V cells to synthesize melanin, thereby improving uneven skin color and reducing skin coloration caused by ultraviolet radiation; at the same time, phenethyl resorcinol can also be used as an antioxidant, can effectively regulate keratin renewal, strengthen the skin keratin barrier, and enhance skin resistance; induce endothelial cell migration, capillary hyperplasia, promote fibroblast proliferation, can effectively rebuild collagen fiber bundles, and restore skin elasticity and toughness. Glutathione encapsulated in cyclodextrin can directly inhibit the activity of tyrosinase by stably binding to the copper-containing active site of the enzyme, regulate the conversion mechanism from eumelanin to pheomelanin, remove free radicals and peroxides that contribute to the activation of tyrosinase and the formation of melanin, and regulate the decolorization ability of melanocyte toxic agents (benzoquinone, o-quinone and other substances), thereby achieving whitening and freckle removal effects; at the same time, vitamin C is used to inhibit the activity of tyrosinase on the one hand, and on the other hand, reversibly hydrogenate or dehydrogenate, so that the oxidized glutathione is reduced to reduced glutathione.

[0052] The above is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments. All technical solutions under the concept of the present invention belong to the protection scope of the present invention. It should be pointed out that for ordinary technicians in this technical field, some improvements and modifications without departing from the principle of the present invention should also be regarded as the protection scope of the present invention.

Claims

1. A freckle-removing and whitening essence, characterized in that: Calculated by mass percentage, the composition includes: 0.5-3.5% whitening and anti-freckle agent, 5-10% moisturizing agent, 2-5% skin conditioning agent, 0.2-0.6% preservative, 0.1-0.3% thickener, 0.1-0.2% pH regulator, 0.02-0.05% chelating agent and the remainder water.

2. The freckle-removing and whitening essence according to claim 1, characterized in that: The whitening and freckle removing agent comprises phenethyl resorcinol, 4-butyl resorcinol, vitamin C and glutathione encapsulated by cyclodextrin.

3. The freckle-removing and whitening essence according to claim 2, characterized in that: The weight ratio of phenethylresorcinol, 4-butylresorcinol, vitamin C and glutathione encapsulated by cyclodextrin is (3-5):(10-25):(5-10):(40-60).

4. The freckle-removing and whitening essence according to claim 3, characterized in that: The preparation method of cyclodextrin-encapsulated glutathione comprises the following steps: adding glutathione into cyclodextrin slurry, stirring, ultrasonicating, freeze-drying, crushing and sieving to obtain glutathione.

5. The freckle-removing and whitening essence according to claim 4, characterized in that: The preparation method of cyclodextrin-encapsulated glutathione comprises the following steps: adding glutathione into cyclodextrin slurry, stirring, ultrasonicating, freeze-drying, crushing and sieving to obtain glutathione.

6. The freckle-removing and whitening essence according to claim 1, characterized in that: The moisturizing agent includes at least one of glycerin, butylene glycol and pentylene glycol.

7. The freckle-removing and whitening essence according to claim 1, characterized in that: The skin conditioning agent includes at least one of a Poria cocos extract, a Rehmannia glutinosa root extract, and a Ginseng root extract.

8. The method for preparing the freckle-removing and whitening essence according to any one of claims 1 to 7, characterized in that: The following steps are involved: S1: Accurately weigh each component according to the formula, add water, humectant, thickener and chelating agent into the container, heat to 85±5℃, homogenize for 5 minutes and keep warm for 15 minutes; S2: After cooling to 40-45°C, add pH regulator and stir evenly; S3: Add the remaining components into the container and stir evenly to obtain the freckle removing and whitening essence.

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