Deinococcus radiodurans-based functional pet food and preparation method thereof

Abstract

The invention provides a functional pet food based on deinococcus radiodurans and a preparation method thereof, and the functional pet food based on deinococcus radiodurans comprises a functional pet food base material and an additive, the mass percentage of the functional pet food base material is 92-99 wt%; the mass percentage of the additive is 1-8wt%; the additive contains carotenoid with the mass percentage of 0.01-0.5 wt%, and the carotenoid is extracted from deinococcus radiodurans. The functional pet food containing the carotenoid with the mass percentage of 0.01-0.5 wt%, provided by the invention, can be beneficial to preventing oxidative damage of brains of middle-aged and aged cats and improving intestinal flora of the middle-aged and aged cats, so that the oxidation resistance of the middle-aged and aged cats is improved, the activity of the middle-aged and aged cats is enhanced, the health of the middle-aged and aged cats is guaranteed, and the aging of the middle-aged and aged

Description

Technical Field

[0001] The invention relates to the technical field of animal food, and in particular to a functional pet food based on radioresistant deinococcin and a preparation method thereof. Background Art

[0002] Pets, such as cats, are gentle companion animals and are loved by people. They can effectively relieve psychological stress and meet emotional needs. However, as they age, the physical functions of middle-aged and elderly pets gradually decline, and their immunity and antioxidant capacity weaken, leading to an increase in free radicals in the body. Due to the reduction in antioxidant capacity, elderly pets find it difficult to remove free radicals in time, become sensitive to external stress, and are easily invaded by diseases, and their vitality also decreases.

[0003] How to improve the antioxidant capacity of pets is one of the important directions of pet food research at this stage. Based on this, the present invention proposes a new functional pet food based on radiation-resistant deinococcin and a preparation method thereof. Summary of the invention

[0004] Based on the above description, the present invention provides a functional pet food based on radioresistant deinococcin and a preparation method thereof, so as to improve the antioxidant capacity of middle-aged and elderly cats, enhance their vitality, ensure their health, and delay their aging.

[0005] The technical solution of the present invention to solve the above technical problems is as follows: In a first aspect, the present invention provides a functional pet food based on radioresistant deinococcin, comprising a functional pet food base and an additive; The mass percentage of the functional pet food base material is 92-99wt%; The mass percentage of the additive is 1-8wt%; the additive contains 0.01-0.5wt% of carotenoids, and the carotenoids are extracted from Deinococcus radiodurans.

[0006] Based on the above technical solution, the present invention can also be improved as follows.

[0007] Furthermore, the additive contains 0.2-0.5wt% carotenoids.

[0008] Furthermore, the additive contains 0.2 wt% of carotenoids.

[0009] Furthermore, the additives also include taurine, DL-methionine, methionine iron complex, methionine copper complex, methionine zinc complex, methionine manganese complex, potassium chloride, yeast selenium, dl-a-tocopherol acetate, niacinamide, vitamin A, vitamin D3, ammonium sulfate nitrate, folic acid, D-calcium pantothenate, riboflavin, pyridoxine hydrochloride, D-biotin, cyanocobalamin and yucca extract.

[0010] Furthermore, the functional pet food base comprises the following raw materials in mass percentage: 54-57% frozen chicken breast, 13-16% frozen leg meat, 5-7% frozen chicken heart, 4-6% frozen chicken liver, 4-6% potato flour, 1-3% pumpkin flour, 0.3-0.5% fish oil, and the rest are chicory root powder and psyllium husk powder.

[0011] Furthermore, the functional pet food base comprises the following raw materials by mass percentage: 56% frozen chicken breast, 15% frozen leg meat, 6% frozen chicken heart, 5% frozen chicken liver, 5% potato flour, 2% pumpkin flour, 0.5% fish oil, and the rest being chicory root powder and psyllium husk powder.

[0012] In a second aspect, the present invention provides a method for preparing a functional pet food based on radioresistant deinococcin as described in the first aspect, comprising the following steps: S1. Proportioning: Frozen chicken breast, frozen leg meat, frozen chicken heart, frozen chicken liver, potato flour, pumpkin flour, fish oil, chicory root powder and psyllium husk powder are mixed to obtain a functional pet food base; S2. Mixing: Mixing the functional pet food base material and the additive in a proportioned manner and stirring evenly; wherein the additive contains 0.01-0.5wt% of carotenoids; S3, molding: transferring the mixed materials to the molding machine for molding; S4, drying: using hot air drying process to dry the formed materials; S5, cooling: the dried material is transferred to the cooling equipment for cooling treatment; The functional pet food based on radioresistant deinococcin is obtained.

[0013] Based on the above technical solution, the present invention can also be improved as follows.

[0014] Furthermore, before step S1, the method further includes: Thawing: thaw the frozen chicken breast, frozen chicken heart and frozen chicken liver; Mixing: Mix the thawed materials through the mincing process; Crushing: crush the frozen chicken thigh meat with an ultra-fine grinder; Add materials: Mix the thawed materials and crushed meat and bones.

[0015] Furthermore, in step S3, a round particle mold is selected for molding.

[0016] Furthermore, in step S4, during the drying process, the temperature of the oven equipment is maintained below 90° C., and the moisture content is monitored every hour. When the moisture content reaches 5%, the drying is completed.

[0017] Compared with the prior art, the technical solution of this application has the following beneficial technical effects: The functional pet food based on radioresistant Deinococcus provided by the present invention comprises a functional pet food base and an additive; the mass percentage of the functional pet food base is 92-99wt%; the mass percentage of the additive is 1-8wt%; the additive contains 0.01-0.5wt% of carotenoids, and the carotenoids are extracted from radioresistant Deinococcus. Radioresistant Deinococcus is a special hydroxylated tetraterpenoid compound synthesized by radioresistant Deinococcus, which exhibits strong antioxidant activity both in vivo and in vitro, and radioresistant Deinococcus can effectively alleviate movement disorders, reduce hippocampal pathological changes, inhibit the activation of microglia and astrocytes, and reduce oxidative stress; in addition, an appropriate amount of radioresistant Deinococcus can restore the intestinal flora of aging animals.

[0018] In summary, the functional pet food provided by the present invention containing 0.01-0.5wt% carotenoids can help prevent oxidative damage to the brain of middle-aged and elderly cats and improve their intestinal flora, thereby improving the antioxidant capacity of middle-aged and elderly cats, enhancing their vitality, ensuring their health, and delaying their aging.

[0019] Since the preparation method of the functional pet food based on radioresistant Deinococcus is used to prepare the above-mentioned functional pet food based on radioresistant Deinococcus, the preparation method has at least all the beneficial effects brought by the functional pet food based on radioresistant Deinococcus, which will not be described one by one here. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions in the present invention or the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0021] Figure 1 This is a diagram showing the effect of intervention with radioresistant deinococcin on the body weight and behavioral performance of D-gal-induced aging mice provided in an embodiment of the present invention; Figure 2This is a diagram showing the effect of intervention with radioresistant deinococcus provided in an embodiment of the present invention on pathological changes in hippocampal tissues of aged mice induced by D-gal; Figure 3 This is a diagram showing the effect of intervention with radioresistant deinococcin on D-gal-induced neurogenesis in the hippocampus of aged mice provided in an embodiment of the present invention; Figure 4 This is a diagram showing the effect of intervention with radioresistant deinococcin on D-gal-induced oxidative stress in the brain and serum of aged mice provided by an embodiment of the present invention; Figure 5 This is a diagram showing the effect of intervention with radioresistant deinococcin on the diversity of intestinal flora in D-gal-induced aged mice provided in an embodiment of the present invention; Figure 6 This is a diagram showing the effect of intervention with radioresistant deinococcus on the composition of intestinal flora in aging mice provided in an embodiment of the present invention. DETAILED DESCRIPTION

[0022] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be clearly and completely described below in conjunction with the drawings of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] The following is combined with Figures 1 to 6 The embodiments of the present invention are further described in detail with examples. The following examples are used to illustrate the present invention but cannot be used to limit the scope of the present invention.

[0024] The functional pet food based on radioresistant deinococcin provided by the invention comprises a functional pet food base material and additives.

[0025] The mass percentage of the functional pet food base material is 92-99wt%.

[0026] Specifically, the functional pet food base comprises the following raw materials in mass percentage: 54-57% frozen chicken breast, 13-16% frozen leg meat, 5-7% frozen chicken heart, 4-6% frozen chicken liver, 4-6% potato flour, 1-3% pumpkin flour, 0.3-0.5% fish oil, and the rest are chicory root powder and psyllium husk powder.

[0027] More preferably, the functional pet food base comprises the following raw materials by mass percentage: 56% frozen chicken breast, 15% frozen leg meat, 6% frozen chicken heart, 5% frozen chicken liver, 5% potato flour, 2% pumpkin flour, 0.5% fish oil, and the rest being chicory root powder and psyllium husk powder.

[0028] The mass percentage of the additive is 1-8wt%; the additive contains 0.01-0.5wt% of carotenoids, and the carotenoids are extracted from Deinococcus radiodurans.

[0029] Preferably, the additive contains 0.2-0.5 wt % carotenoids.

[0030] In addition, the above-mentioned additives also include taurine, DL-methionine, methionine iron complex, methionine copper complex, methionine zinc complex, methionine manganese complex, potassium chloride, yeast selenium, dl-a-tocopherol acetate, nicotinamide, vitamin A, vitamin D3, ammonium nitrate, folic acid, D-calcium pantothenate, riboflavin, pyridoxine hydrochloride, D-biotin, cyanocobalamin and yucca extract. The content of this part of the raw materials can be adaptively formulated according to the basic ratio rules in the field, without specific limitation. The core point of this application is the carotenoids in the additives and the corresponding radioresistant Deinococcus.

[0031] The following is an example of cat food and is described in conjunction with specific embodiments: Example 1 This embodiment provides a method for preparing a functional pet food based on radioresistant deinococcin and the corresponding functional pet food (cat food), and the preparation method is as follows: Step S1, thawing: thawing the frozen chicken breast, frozen chicken heart and frozen chicken liver at a temperature of -5 to 5°C for 5 to 10 hours.

[0032] Step S2, mixing: the thawed materials are mixed through a meat mincing process.

[0033] Step S3, crushing: crush the frozen chicken thigh meat with an ultra-fine grinder.

[0034] Step S4, feeding: mixing the mixed thawed material and the crushed meat and bones.

[0035] Step S5, proportioning: Frozen chicken breast, frozen leg meat, frozen chicken heart, frozen chicken liver, potato flour, pumpkin flour, fish oil, chicory root powder and psyllium husk powder are proportioned and mixed to obtain a cat food base.

[0036] Step S6, mixing: mixing the cat food base material and the additive in a proportioned manner and stirring evenly; wherein the additive contains 0.2wt% of carotenoids by weight.

[0037] Step S7, molding: select a round particle mold and transfer the mixed material to a molding machine for molding.

[0038] Step S8, drying: adopt hot air drying process to dry the formed material; during the drying process, the temperature of the oven equipment is maintained below 90°C, and the moisture content is monitored every 1 hour. When the moisture content reaches 5%, the drying is completed.

[0039] It should be noted that carotenoids (extracted from Deinococcus radiodurans) will lose some of their activity at high temperatures. Therefore, the 90°C hot air baking process will retain more activity of carotenoids (extracted from Deinococcus radiodurans) compared to the puffing process (120°C, 30 minutes).

[0040] Step S9, cooling: the dried material is transferred to a cooling device for cooling treatment to a temperature below 50°C.

[0041] A cat food based on radioresistant deinococcin is obtained, wherein the cat food base is: 56% frozen chicken breast, 15% frozen leg meat, 6% frozen chicken heart, 5% frozen chicken liver, 5% potato flour, 2% pumpkin flour, 0.5% fish oil (from salmon), and the rest is chicory root powder and psyllium husk powder; The additives are: carotenoids (extracted from Deinococcus radiodurans) 0.2wt%, taurine, DL-methionine, methionine iron complex, methionine copper complex, methionine zinc complex, methionine manganese complex, potassium chloride, yeast selenium, dl-a-tocopheryl acetate, niacinamide, vitamin A, vitamin D3, ammonium sulfate nitrate, folic acid, D-calcium pantothenate, riboflavin (vitamin B2), pyridoxine hydrochloride, D-biotin, cyanocobalamin (vitamin B12), and yucca extract.

[0042] Example 2 The preparation method of the functional pet food based on radioresistant Deinococcus provided in this embodiment and the corresponding functional pet food (cat food) obtained are different from those in Example 1 in that the mass proportion of carotenoids (extracted from Deinococcus radioresistant) in the additive is 0.5wt%.

[0043] Comparative Example 1 The difference from the above-mentioned Example 1 is that this comparative example does not contain carotenoids (extracted from Deinococcus radiodurans), and the remaining ingredients and the corresponding preparation process are the same as those of Example 1.

[0044] Comparative Example 2 The difference from the above-mentioned Example 1 is that the mass proportion of carotenoids (extracted from Deinococcus radiodurans) in the additive in this comparative example is 0.6wt%, which exceeds the ideal range of 0.01~0.5wt%, and the remaining ingredients and the corresponding preparation process are the same as those in Example 1.

[0045] The cat food obtained from the above examples and comparative examples was investigated for performance: Sixteen middle-aged and elderly British shorthair castrated male cats with an average age of 8 years were selected as test subjects (weight 5 ± 0.50 kg), divided into four groups, and placed in four rooms respectively. They were allowed to move freely indoors, have free access to water, and receive natural light. They were fed regularly every day at the same feeding time (once at 8 am and once at 6 pm). The feeding cycle was 28 days, including 14 days of adaptive feeding and 14 days of experimental phase.

[0046] Feces were collected on days 0 and 14; they were collected within 15 minutes after defecation, scored for morphology, and weighed and stored at -20°C.

[0047] Data Collection: ① Growth performance indicators: palatability, feed intake, body weight and feces ②Intestinal flora sequencing indicators The test results are as follows: (1) Palatability, feeding situation, body weight and feces: In terms of average feeding amount, the feeding amount of the four groups was relatively close, with no significant difference, and the palatability was also normal. There was no decrease in feeding amount in the later stage of feeding, indicating that the addition of carotenoids (extracted from radioresistant Deinococcus) had no significant effect on the palatability of cat food. In terms of average body weight, the cats that ate the cat food obtained in Example 1 and Example 2 all increased, with an average increase of 0.08kg in the Example 1 group, an average increase of 0.07kg in the Example 2 group, an average increase of 0.03kg in the Comparative Example 1 group, and an average increase of 0.02kg in the Comparative Example 2 group; the feces were all normal. It can be seen that the palatability of Comparative Example 2 was relatively low, which was the effect of the continuous addition of carotenoids (extracted from radioresistant Deinococcus). That is, when the amount of carotenoids (extracted from radioresistant Deinococcus) added exceeded the ideal range, the low palatability affected the cat's feeding amount and its weight gain.

[0048] Table 1. Effects of different cat food formulas on cat growth indicators

[0049] (2) In terms of intestinal flora, it can be seen that the intestinal flora balance, flora diversity and number of bacterial species in Examples 1 and 2 are better than those in Comparative Examples 1 and 2; among them, Comparative Example 1 does not add carotenoids (extracted from Deinococcus radiodurans), and Comparative Example 2 adds a relatively high dose. It can be seen that the high dose addition of Comparative Example 2 affects the palatability and the feed intake is lower than that of other groups. In essence, there is no significant difference in the intestinal flora effect between the addition amounts of 0.5% and 0.6%, but excessive addition of carotenoids (extracted from Deinococcus radiodurans) leads to a decrease in feed intake. Due to the different feed intake, the amount of nutrients absorbed is different. On the contrary, the overall intestinal flora index of Comparative Example 2 is not as good as that of Example 2, and the overall intestinal flora index of Example 2 is not as good as that of Example 1, indicating that the beneficial effect of high-dose addition on intestinal flora is generally not as good as that of low-dose.

[0050] Table 2. Effects of different cat food formulas on intestinal flora indicators of cats

[0051] In addition, further, regarding the effects of carotenoids (extracted from Deinoxanthin) on animals, especially the effects on antioxidant activity and intestinal flora, the present invention also provides corresponding mouse experimental verification, exploring the potential beneficial effects of Deinoxanthin (DX) on D-galactose (D-gal)-induced aged mice, especially its protective effect on the brain from oxidative damage, and its effects on the intestinal flora of aged mice: (including three treatment test groups: LD group low dose (25 mg / kg / day), MD group medium dose (50 mg / kg / day) and HD group high dose (100 mg / kg / day)) 1. Effects of DX intervention on body weight and behavioral performance of D-gal-induced aging mice Schematic diagram of animal experiment Figure 1 As shown in A, during the intervention period, one mouse in the model group (group M) died due to physical damage caused by intraperitoneal injection, intragastric administration or other unknown factors. At the end of DX treatment, the body weight of mice in group M was significantly lower than that of the normal control group (group C) (p < 0.01), indicating that D-gal-induced aging of mice has a great effect on the body weight of mice ( Figure 1 B); low-dose and medium-dose DX treatment can effectively inhibit the weight loss of mice caused by D-gal; among them, compared with the M group, the body weight of mice in the medium-dose group (MD group) returned to normal levels (p<0.01), while high-dose DX treatment seemed to slightly aggravate the weight loss of elderly mice.

[0052] Subsequently, the mice were evaluated for behavioral changes using three behavioral tests.

[0053] The results of the open field test showed that the total distance moved by mice in the model group (group M) was shorter and the rest time was longer than that in the normal control group (group C). Figure 1 C and D ), showing abnormal exploratory behavior induced by D-gal in aged mice.

[0054] The pole climbing test showed that mice in group M exhibited motor impairment compared with group C, characterized by a significant increase in the time required for mice to return to the bottom of the rough surface ( Figure 1E ).

[0055] In addition, the hanging test showed that mice in group M did not place their hind limbs on the wire and only used their forelimbs to hang their bodies, resulting in a rapid decrease in their own hanging time scores ( Figure 1F ).

[0056] Given that differences in body weight may affect the performance of animals in the hanging test, the product of time score (s) and body weight (g) was used to further analyze the body impulse of each mouse. As shown in Figure 1G, mice in group M showed significantly reduced body impulse, indicating that the induction of D-gal may cause overall muscle dysfunction in mice.

[0057] In these tests, the performance of mice in the low-dose group (LD group) and the medium-dose group (MD group) was much better than that of the M group, especially the performance of the MD group was as good as that of the C group, indicating that the appropriate amount of DX treatment effectively improved the exploration activity, motor ability and overall muscle function of D-gal-induced aged mice. Similarly, the behavioral performance of mice in the high-dose group (HD group) was still significantly weaker than that of the C group, and even slightly worse than that of the M group, indicating that excessive intake of DX further aggravated the motor incoordination of aged mice.

[0058] 2. Effects of DX on D-gal-induced pathological changes in hippocampal tissues of aged mice HE staining was used to observe the histopathological changes of the hippocampus. The HE staining results of the dentate gyrus (DG), cornu 1 (CA1), and cornu 2 (CA2) of the hippocampus are shown in Figure 2. Figure 2 As shown. The cells in the DG, CA1, and CA2 regions of the hippocampus of group C mice had complete morphology, uniform size, orderly arrangement, and distinct layers; the chromatin was evenly distributed, and the shape of the nucleus was clearly visible. In contrast, some cells in the hippocampus of group M mice had angular or irregular nuclei, especially those in the CA2 region, accompanied by darker staining, reduced cell density, and disorganized or loose arrangement; there was some cell loss in the DG and CA1 regions, characterized by thinner cell layers; in the treatment groups, especially the MD group, the overall cells showed the same normal morphology and arrangement as those in group C.

[0059] However, a large number of cells in the CA2 region of the LD and HD groups still maintained the same irregular nuclei, hyperchromasia, and lower cell density as those in the M group. The number of cells with changes in the CA2 region appeared to be increased in the HD group compared with the M group. These results suggest that treatment with an appropriate amount of DX can alleviate the hippocampal pathological changes induced by D-gal in aged mice.

[0060] Effects of DX on D-gal-induced neurogenesis in the hippocampus of aged mice from Figure 3 As can be seen in the figure, compared with group C, GFAP-immunoreactive astrocytes and Iba-1-immunoreactive microglia in the DG region of the hippocampus of group M mice were induced to activate by D-gal, which was characterized by enlarged cell bodies and thickened staining ( Figure 3 A).

[0061] The degree of positive cells was quantitatively analyzed using histochemical score (H-score) and integrated optical density (IOD). The results showed that the H-value (Fig. 3B and C) and IOD value (Fig. 3D and F) of GFAP-positive and Iba-1-positive cells in group M were significantly higher than those in group C, indicating that the corresponding cells were activated by D-gal. Compared with group M, appropriate DX administration could effectively inhibit the activation of GFAP- and Iba-1-positive cells in a dose-dependent manner. However, high-dose DX treatment promoted further activation of these cells. These results suggest that DX may be considered a key regulator for improving D-gal-induced hippocampal neurogenesis in aged mice.

[0062] Effects of DX on D-gal-induced oxidative stress in the brain and serum of aged mice The activities of T-SOD and GSH-Px in the serum and brain of each group of mice and the level of MDA were measured to evaluate the protective effect of DX on D-gal-induced aging mice (Figure 4). Compared with the mice in the C group, the activities of T-SOD and GSH-Px in the serum and brain of the mice in the M group were significantly decreased; on the contrary, the level of MDA showed a significant increase (p<0.01), indicating that continuous DX stimulation caused severe oxidative stress in mice.

[0063] However, different concentrations of DX treatment showed different antioxidant effects. In more detail, low-dose DX treatment slightly affected the activities of T-SOD and GSH-Px and MDA levels in the serum and brain of aged mice (p>0.05); medium-dose DX treatment could significantly reverse the changes of these antioxidant indicators in the brain and serum of aged mice (p<0.05); and high-dose DX treatment seemed to aggravate D-gal-induced oxidative damage in aged mice, although there was no significant difference between the M group and the HD group (p>0.05).

[0064] 5. Effect of DX on the diversity of intestinal flora in D-gal-induced aging mice Chao1 index, Shannon index and Simpson index were selected to reflect the changes in the α diversity of intestinal flora in different groups (Figure 5A-C). The results showed that intraperitoneal injection of D-gal to construct an aged mouse model had no significant effect on the α diversity of intestinal flora, as there was no statistical significance between groups C and M. However, DX treatment reduced the α diversity of the intestinal microbiota of aged mice induced by D-gal in a dose-dependent manner, especially the high dose significantly reduced the α diversity (p<0.05).

[0065] PCoA and Anosim analysis were used to explore the β diversity of the intestinal microbiota. The results showed that the distance between the sample clusters in group M was far from that in group C, indicating that the diversity of the intestinal microbiota was changed by D-gal induction ( Figure 5 D). The LD and MD groups clustered closer to group C, indicating that the gut microbiota diversity between these groups was more similar ( Figure 5 D).

[0066] It is noteworthy that the distances of the sample clusters in the HD group were different from those in the other groups, further indicating that high-dose DX significantly affected the diversity of the intestinal microbiota in D-gal-induced aged mice. In addition, Anosim analysis demonstrated that the differences between groups were greater than those within the groups (R = 0.476), supporting the significance of our grouping (p < 0.05) (Figure 5E).

[0067] 6. Effect of DX on the composition of intestinal flora in aged mice like Figure 6 As shown, the OTUs Venn diagram shows that the total number of OTUs in the C, M, LD, MD, and HD groups are 695, 620, 677, 632, and 649, respectively, with a total of 416 OTUs, and the number of unique OTUs in each group is 55, 23, 46, 36, and 37, respectively ( Figure 6 A).

[0068] Figure 6 B and C show the relative abundance of intestinal flora in each group at the phylum and genus level, respectively. At the phylum level, the intestinal flora of each group was dominated by Bacteroidetes, Firmicutes, and Proteobacteria, accounting for more than 95% of the total flora. Compared with group C, the relative abundance of Bacteroidetes and Proteobacteria in group M increased by 5.32% and 2.38%, respectively, while the relative abundance of Firmicutes decreased by 7.2%. Low-dose DX treatment restored the changes in flora at the phylum level.

[0069] It is worth noting that in the HD group, the relative abundances of Bacteroidetes and Firmicutes were 75.88% and 17.26%, respectively, showing a clear upward and downward trend compared with the other groups ( Figure 6 B).

[0070] At the genus level, a total of 43 genera showed significant differences between these groups, especially Prevotella, Ruminococcus, and Lactobacillus ( Figure 6 C).

[0071] Among the top 20 genera with the most changes, the relative abundance of Prevotella, Clostridium IV, Roseburia, Eisenbergia, and Turicibacter decreased in group M compared with group C; whereas Alloprevotella, Lactobacillus, Ruminococcus, Escherichia coli / Shigella, and Veillonella were enriched in group M ( Figure 6 D).

[0072] Treatment with low- and medium-dose DX restored the changes in these genera, especially low-dose DX even restored them to normal levels. However, high-dose DX administration excessively reversed the relative abundance of these genera, as evidenced by the changes in Prevotella, Escherichia coli / Shigella, Treponema, and Butyrobacterium. These results suggest that the administration of appropriate DX can restore the disordered intestinal flora of D-galactose-induced aged mice.

[0073] In summary, it can be seen that carotenoids (extracted from Deinococcus radiodurans) have certain effects on animals, especially in terms of antioxidant activity and intestinal flora. Specifically, low-dose (25 mg / kg / day) and medium-dose (50 mg / kg / day) DX treatment can effectively alleviate movement disorders, reduce hippocampal pathological changes, inhibit microglia and astrocyte activation, and reduce D-Gal-induced oxidative stress in aged mice; however, high-dose DX (100 mg / kg / day) treatment seems to aggravate these changes, indicating that excessive use of DX may promote rather than prevent oxidative damage in aged mice. In addition, taking appropriate DX can restore the intestinal flora of aged mice, while high-dose DX will further aggravate the disorder of the intestinal flora of aged mice.

[0074] In conclusion, it can be concluded that appropriate administration of DX may help prevent oxidative damage in the brain and improve the intestinal flora in aged mice.

[0075] Finally, combined with the above analysis, compared with the prior art and the comparative example, the functional pet food based on radiation-resistant deinococcin and the preparation method thereof provided in the embodiments of the present invention can achieve the following technical effects: the functional pet food containing 0.01~0.5wt% carotenoids provided by the present invention can help prevent oxidative damage to the brain of middle-aged and elderly pets and improve their intestinal flora, thereby improving the antioxidant capacity of middle-aged and elderly pets, enhancing their vitality, ensuring their health, and delaying their aging.

[0076] In the description of this specification, the description with reference to the terms "specific example" or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the embodiment of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example.

[0077] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A functional pet food based on radioresistant deinococcin, characterized in that: Includes functional pet food bases and additives; The mass percentage of the functional pet food base material is 92-99wt%; The mass percentage of the additive is 1-8wt%; the additive contains 0.01-0.5wt% of carotenoids, and the carotenoids are extracted from Deinococcus radiodurans.

2. The functional pet food based on radioresistant deinococcin according to claim 1, characterized in that: The additive contains 0.2-0.5wt% of carotenoids.

3. The functional pet food based on radioresistant deinococcin according to claim 2, characterized in that: The additive contains 0.2 wt% of carotenoids.

4. The functional pet food based on radioresistant deinococcin according to any one of claims 1 to 3, characterized in that: The additives also include taurine, DL-methionine, methionine iron complex, methionine copper complex, methionine zinc complex, methionine manganese complex, potassium chloride, yeast selenium, dl-a-tocopheryl acetate, niacinamide, vitamin A, vitamin D3, ammonium sulfate nitrate, folic acid, D-calcium pantothenate, riboflavin, pyridoxine hydrochloride, D-biotin, cyanocobalamin and yucca extract.

5. The functional pet food based on radioresistant deinococcin according to claim 1, characterized in that: The functional pet food base comprises the following raw materials in percentage by mass: 54-57% frozen chicken breast, 13-16% frozen leg meat, 5-7% frozen chicken heart, 4-6% frozen chicken liver, 4-6% potato flour, 1-3% pumpkin flour, 0.3-0.5% fish oil, and the rest being chicory root powder and psyllium husk powder.

6. The functional pet food based on radioresistant deinococcin according to claim 5, characterized in that: The functional pet food base comprises the following raw materials by mass percentage: 56% frozen chicken breast, 15% frozen leg meat, 6% frozen chicken heart, 5% frozen chicken liver, 5% potato flour, 2% pumpkin flour, 0.5% fish oil, and the rest being chicory root powder and psyllium husk powder.

7. A method for preparing a functional pet food based on radioresistant deinococcin according to any one of claims 1 to 6, characterized in that: The steps include: S1. Proportioning: Frozen chicken breast, frozen leg meat, frozen chicken heart, frozen chicken liver, potato flour, pumpkin flour, fish oil, chicory root powder and psyllium husk powder are mixed to obtain a functional pet food base; S2. Mixing: Mixing the functional pet food base material and the additive in a proportioned manner and stirring evenly; wherein the additive contains 0.01-0.5wt% of carotenoids; S3, molding: transferring the mixed materials to the molding machine for molding; S4, drying: using hot air drying process to dry the formed materials; S5, cooling: the dried material is transferred to the cooling equipment for cooling treatment; The functional pet food based on radioresistant deinococcin is obtained.

8. The preparation method according to claim 7, characterized in that: Before step S1, the method further includes: Thawing: thaw the frozen chicken breast, frozen chicken heart and frozen chicken liver; Mixing: Mix the thawed materials through the mincing process; Crushing: crush the frozen chicken thigh meat with an ultra-fine grinder; Add materials: Mix the thawed materials and crushed meat and bones.

9. The preparation method according to claim 7, characterized in that: In step S3, a round-grained mold is selected for molding.

10. The preparation method according to claim 7, characterized in that: In step S4, during the drying process, the temperature of the oven equipment is maintained below 90°C, and the moisture content is monitored every hour. When the moisture content reaches 5%, the drying is completed.

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