Plasma matrix and preparation method of plasma matrix bone block
By controlling the centrifugal conditions of whole blood samples, solid and liquid plasma matrix were obtained and mixed with them, the problems of diverse plasma matrix preparation processes and unstable morphology were solved, and plasma matrix with high mechanical strength and good bone regeneration ability were prepared. Bone blocks significantly improve clinical treatment effect.
Patent Information
- Application Number
- CN202411332013.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-24
- Publication Date
- 2024-12-27
AI Technical Summary
The preparation process of plasma matrix is diverse, resulting in unstable morphology, high fluidity of liquid plasma matrix, low strength after coagulation, fast degradation, and limited ability to promote bone regeneration in bone regeneration materials.
A standardized preparation method is provided by controlling the centrifugal radius and rate of whole blood samples, solid and liquid plasma matrix are obtained, respectively, and mixed to prepare plasma matrix bone blocks.
It improves the mechanical strength of plasma matrix bone mass, slow degradation speed, strong bone regeneration ability, is suitable for hard tissue defects, and significantly improves clinical treatment effect.
Smart Images
Figure CN119185659A_ABST
Abstract
Description
Technical Field
[0001] The present disclosure relates to the field of biomedicine technology, and in particular to a method for preparing a plasma matrix and a plasma matrix bone block. Background Art
[0002] Plasma matrix is a concentrated extract derived from human autologous blood. As an emerging bioregenerative material, it has attracted much attention. It also has good biocompatibility and biosafety. The main component of plasma matrix is platelets, which can play a strong procoagulant role after being successfully activated. More importantly, plasma matrix contains rich growth factors, including transforming growth factor (TGF-β), vascular endothelial growth factor (VEGF), platelet-derived growth factor (PGDF), etc., and the preparation is simple and has no rejection reaction. It has developed rapidly in recent years and has been widely used in wound healing, bone regeneration barrier membranes, bone regeneration materials and soft tissue repair with good results. However, the disadvantages of liquid plasma matrix are also obvious. It has high fluidity, low strength after coagulation, and rapid degradation. When mixed with bone regeneration materials, the ability of liquid plasma matrix to promote bone regeneration is also limited.
[0003] At present, there are various preparation processes of plasma matrix, the morphology of the products after centrifugation is different, and there is no unified standard for how to use plasma matrix to prepare bone blocks. Therefore, it is urgent to provide a plasma matrix and a preparation method of plasma matrix bone blocks to improve their clinical application effect. Summary of the invention
[0004] In order to solve the above technical problems, the present disclosure provides a plasma matrix and a method for preparing a plasma matrix bone block.
[0005] In a first aspect, the present disclosure provides a method for preparing a plasma matrix, wherein the plasma matrix includes a liquid plasma matrix and a solid plasma matrix, and the preparation method comprises the following steps:
[0006] At least two whole blood samples are centrifuged using a plasma matrix platform to obtain a liquid plasma matrix and a solid plasma matrix, respectively; wherein the centrifugal radius R1 of the whole blood sample used to obtain the solid plasma matrix is greater than the centrifugal radius R2 of the whole blood sample used to obtain the liquid plasma matrix.
[0007] The whole blood sample described in the present disclosure is collected in a plasma matrix blood collection tube, and the blood collection tube includes but is not limited to metal materials, plastic materials, glass materials, etc.
[0008] After centrifugation, the whole blood sample is divided into three layers, including an upper light yellow plasma matrix layer, a middle brown-yellow layer and a lower red blood cell layer. In the present disclosure, by centrifuging different whole blood samples to stratify the whole blood samples, two different forms of plasma matrix layers (liquid and solid) can be obtained.
[0009] The present invention controls the centrifugal radius of different whole blood samples and centrifuges different whole blood samples at different centrifugal radii at the same time. After the centrifugation, solid and liquid plasma matrix products can be obtained respectively. The preparation method provided by the present invention solves the problem of unstable morphology of plasma matrix in current preparation.
[0010] As a preferred technical solution of the present disclosure, in the preparation method, the difference between R1 and R2 is 10-500 mm, for example, 20 mm, 100 mm, 180 mm, 260 mm, 340 mm, 420 mm, etc.
[0011] As a preferred technical solution of the present disclosure, in the preparation method, the centrifugal radius R1 of the whole blood sample used to obtain the solid plasma matrix is 10-300 mm, such as 20 mm, 50 mm, 100 mm, 150 mm, 200 mm, 250 mm, etc.
[0012] And / or, the centrifugation radius R2 of the whole blood sample used to obtain the liquid plasma matrix is 10-300 mm, such as 20 mm, 50 mm, 100 mm, 150 mm, 200 mm, 250 mm, etc.
[0013] As a preferred technical solution of the present disclosure, the centrifugal speed is 50-5000g, for example, 100g, 800g, 1500g, 2200g, 2900g, 3600g, 4300g, etc.
[0014] As a preferred technical solution of the present disclosure, the centrifugation time is 1-60 min, such as 5 min, 15 min, 25 min, 35 min, 45 min, 55 min, etc.
[0015] In the present disclosure, since different whole blood samples are actually centrifuged simultaneously using the same plasma matrix platform, for the preparation methods of the liquid plasma matrix and the solid plasma matrix obtained in the present disclosure, the two only differ in the centrifugal radius, and the centrifugal rate and centrifugal time are the same parameters.
[0016] As a preferred technical solution of the present disclosure, the whole blood sample is a liquid whole blood sample.
[0017] As a preferred technical solution of the present disclosure, the centrifugation is carried out in a horizontal centrifuge device.
[0018] In a second aspect, the present disclosure provides application of the preparation method described in the first aspect in soft tissue regeneration materials, hard tissue regeneration materials, wound healing materials or plastic surgery materials.
[0019] The plasma matrix obtained in the present invention has good biocompatibility and biosafety, and can be applied to soft tissue regeneration materials, hard tissue regeneration materials, wound healing materials or plastic surgery materials; at the same time, the plasma matrix obtained in the present invention has good effect when mixed with bone regeneration materials.
[0020] In a third aspect, the present disclosure provides a method for preparing a plasma matrix bone block, the preparation method comprising the following steps:
[0021] (1) preparing a liquid plasma matrix and a solid plasma matrix using the plasma matrix preparation method described in the first aspect;
[0022] (2) The solid plasma matrix, the liquid plasma matrix and the bone repair material are mixed to obtain the plasma matrix bone block.
[0023] As a preferred technical solution of the present disclosure, the mixing method includes but is not limited to cutting, mixing and injection.
[0024] The preparation method of the plasma matrix bone block provided in the present disclosure can be used as a standardized preparation process to unify the preparation standards of the plasma matrix bone block.
[0025] In the preparation method of the plasma matrix bone block provided by the present disclosure, liquid and solid plasma matrices are used simultaneously, and a variety of clinical bone replacement materials can be prepared into plasma matrix bone blocks with higher mechanical strength.
[0026] As a preferred technical solution of the present disclosure, the mixing method includes: preparing a plasma matrix membrane from a solid plasma matrix and crushing it, then mixing it with a bone repair material, adding a liquid plasma matrix and letting it stand to obtain the plasma matrix bone block.
[0027] The bone replacement materials disclosed herein include, but are not limited to, autologous bone, allogeneic bone replacement materials, xenogeneic bone replacement materials, artificial synthetic materials, and the like.
[0028] In a fourth aspect, the present disclosure provides a plasma matrix bone block prepared by the preparation method described in the third aspect.
[0029] The plasma matrix bone block prepared by the plasma matrix bone block preparation method provided in the present disclosure can promote bone regeneration, has low fluidity, slow degradation rate, high mechanical strength, is convenient for clinical operation, and has good space maintenance effect. Therefore, it can be used for various types of hard tissue defects and significantly improve the clinical treatment effect.
[0030] Compared with the prior art, the technical solution provided by the embodiments of the present disclosure has the following advantages:
[0031] 1. The plasma matrix preparation method provided by the present disclosure controls the centrifugal radius of different whole blood samples and centrifuges them simultaneously on the same plasma matrix preparation platform. After the centrifugation, solid and liquid plasma matrices can be obtained simultaneously, which solves the problem that the current plasma matrix preparation process is diverse and the product forms are different after centrifugation.
[0032] 2. The plasma matrix bone block preparation method provided by the present disclosure solves the problems of high fluidity of liquid plasma matrix, low strength after coagulation, rapid degradation and limited ability to promote bone regeneration by mixing solid and liquid plasma matrix products. It can be used as a standardized preparation method for plasma matrix bone blocks;
[0033] 3. The plasma matrix bone block provided by the present disclosure has higher mechanical strength, slow degradation rate, improved ability to promote bone regeneration, convenient clinical operation, and improved clinical treatment effect. BRIEF DESCRIPTION OF THE DRAWINGS
[0034] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the present disclosure and, together with the description, serve to explain the principles of the present disclosure.
[0035] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative labor.
[0036] Figure 1 This is a photograph of the solid plasma matrix after centrifugation as described in Example 1 of the present disclosure;
[0037] Figure 2 This is a photograph of the liquid plasma matrix after centrifugation described in Example 1 of the present disclosure;
[0038] Figure 3 This is a photo of the plasma matrix bone block prepared by the preparation method described in Example 2 of the present disclosure. DETAILED DESCRIPTION
[0039] In order to more clearly understand the above-mentioned objectives, features and advantages of the present disclosure, the scheme of the present disclosure will be further described below. It should be noted that the embodiments of the present disclosure and the features in the embodiments can be combined with each other without conflict.
[0040] In the following description, many specific details are set forth to facilitate a full understanding of the present disclosure, but the present disclosure may also be implemented in other ways different from those described herein; it is obvious that the embodiments in the specification are only part of the embodiments of the present disclosure, rather than all of the embodiments.
[0041] Example 1
[0042] This embodiment provides a method for preparing a plasma matrix, and the preparation method is as follows:
[0043] (1) Collecting 6 liquid whole blood samples using glass blood collection tubes, including 4 blood collection tubes for preparing solid plasma matrix and 2 blood collection tubes for preparing liquid plasma matrix;
[0044] (2) centrifuging the collected whole blood samples simultaneously at 700 g for 8 min using a plasma matrix platform to obtain liquid plasma matrix and solid plasma matrix respectively;
[0045] The centrifugal radius R1 of the whole blood sample used to obtain the solid plasma matrix is 50 mm, and the centrifugal radius R2 of the whole blood sample used to obtain the liquid plasma matrix is 30 mm, and R1-R2=20 mm.
[0046] The solid plasma matrix obtained is Figure 1 As shown, the liquid plasma matrix obtained is as Figure 2 As shown by Figure 1 and Figure 2 It can be seen that the preparation method provided by the present disclosure can simultaneously obtain solid plasma matrix and liquid plasma matrix, which solves the problem that the current preparation process of plasma matrix is diverse and the product morphology is different after centrifugation.
[0047] This embodiment also provides applications of plasma matrix, wherein the solid plasma matrix can be used for regeneration of soft and hard tissues, and the liquid plasma matrix can be used for flushing of infected areas, etc.
[0048] Example 2
[0049] This embodiment provides a method for preparing a plasma matrix bone block, and the preparation method is as follows:
[0050] (1) Clamping four solid plasma matrices prepared by the preparation method in Example 1, and extracting the pale yellow plasma matrix layer on the upper layers of two liquid plasma matrices prepared by the preparation method in Example 1;
[0051] (2) Compress the solid plasma matrix into a plasma matrix membrane in a plasma matrix compression device box, then cut the pieces into pieces with a diameter of about 1-2 mm. Mix the pieces with 0.5 g of Bio-Oss bone substitute material, add the extracted liquid plasma matrix and let it stand for 5 min to obtain plasma matrix bone blocks. Figure 3 shown.
[0052] Depend on Figure 3 It can be seen that the plasma matrix bone block obtained by the preparation method provided in the present disclosure has low fluidity and is convenient for clinical operation.
[0053] Comparative Example 1
[0054] This comparative example provides a bone substitute material, which is the bone repair material in Example 2.
[0055] Compared with the bone repair material provided in Comparative Example 1, the plasma matrix bone block obtained in Example 2 of the present disclosure can convert the granular material into a block, and has a significant improvement in morphology. The plasma matrix bone block can promote bone regeneration, and has significantly reduced fluidity, slow degradation rate, high mechanical strength, convenient clinical operation, good space maintenance effect, and can be combined with bone replacement materials. It can be used for various types of hard tissue defects and significantly improve the clinical treatment effect.
[0056] It should be noted that, in this article, relational terms such as "first" and "second" are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "comprise a ..." do not exclude the existence of other identical elements in the process, method, article or device including the elements.
[0057] The above description is only a specific embodiment of the present disclosure, so that those skilled in the art can understand or implement the present disclosure. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present disclosure. Therefore, the present disclosure will not be limited to the embodiments described herein, but will conform to the widest scope consistent with the principles and novel features disclosed herein.
Claims
1. A method for preparing a plasma matrix, characterized in that: The plasma matrix includes a liquid plasma matrix and a solid plasma matrix, and the preparation method includes the following steps: At least two whole blood samples are centrifuged using a plasma matrix platform to obtain a liquid plasma matrix and a solid plasma matrix, respectively; The centrifugal radius R1 of the whole blood sample used to obtain the solid plasma matrix is greater than the centrifugal radius R2 of the whole blood sample used to obtain the liquid plasma matrix.
2. The preparation method according to claim 1, characterized in that: In the preparation method, the difference between R1 and R2 is 10-500 mm.
3. The preparation method according to claim 1 or 2, characterized in that: In the preparation method, the centrifugation radius R1 of the whole blood sample used to obtain the solid plasma matrix is 10-300 mm; And / or, the centrifugation radius R2 of the whole blood sample used to obtain the liquid plasma matrix is 10-300 mm.
4. The preparation method according to any one of claims 1 to 3, characterized in that The centrifugation rate is 50-5000 g.
5. The preparation method according to any one of claims 1 to 3, characterized in that: The centrifugation time is 1-60 min.
6. The preparation method according to any one of claims 1 to 5, characterized in that: The whole blood sample is a liquid whole blood sample.
7. Use of the preparation method according to any one of claims 1 to 6 in soft tissue regeneration materials, hard tissue regeneration materials, wound healing materials or plastic surgery materials.
8. A method for preparing a plasma matrix bone block, characterized in that: The preparation method comprises the following steps: (1) preparing a liquid plasma matrix and a solid plasma matrix using the preparation method according to any one of claims 1 to 6; (2) The solid plasma matrix, the liquid plasma matrix and the bone repair material are mixed to obtain the plasma matrix bone block.
9. The preparation method according to claim 8, characterized in that: In step (2), the mixing method includes: preparing a plasma matrix membrane from a solid plasma matrix and crushing it, then mixing it with a bone repair material, adding a liquid plasma matrix and letting it stand to obtain the plasma matrix bone block.
10. The plasma matrix bone block prepared by the preparation method according to claim 8 or 9.