Composition containing sodium hyaluronate and DNA sodium and application thereof

Abstract

The invention discloses a composition containing sodium hyaluronate and DNA sodium and application of the composition, and belongs to the technical field of biology. The composition is prepared from the following raw materials: DNA sodium, sodium hyaluronate and mannitol, the preparation method of the DNA sodium comprises the following steps: adding a tissue into alkali liquor, and grinding to prepare slurry; adding an enzyme solution into the slurry for enzymolysis to obtain an enzymatic hydrolysate; adding HCl into the enzymatic hydrolysate, carrying out ultrasonic treatment, centrifuging, and taking supernate; and carrying out ultrafiltration on the supernatant, and drying the filtrate to obtain the DNA sodium. The endotoxin of the prepared DNA sodium is lower than 0.5 EU / mg, compared with the prior art, the safety is better, the composition is compounded by the sodium hyaluronate, the mannitol and the DNA sodium, on one hand, the stability of the DNA sodium in a liquid state can be improved, on the other hand, the anti-aging effect of the DNA sodium can be greatly improved, and a technical support is provided for application of the DNA sodium in the field of medical beauty.

Description

Technical Field

[0001] The invention relates to the field of biotechnology, and in particular to a composition containing sodium hyaluronate and sodium DNA and application thereof. Background Art

[0002] Sodium DNA, also known as polydeoxyribonu-cleo tide (PDRN), is a low molecular weight DNA derivative of natural origin, with a molecular weight ranging from 50-1500kDa, and the most representative molecular weight of PDRN is 80-200kDa. It has multiple pharmacological activities such as tissue repair, anti-inflammation, promotion of wound healing, stimulation of angiogenesis, and anti-ischemic injury, and is widely used in supporting, repairing or replacing damaged cells, tissues and organs.

[0003] Studies have shown that the extraction and optimization of PDRN with different molecular weights can be applied to various fields to exert its powerful anti-inflammatory, repair and regeneration effects: 700KDa and above are used for skin regeneration, joint cartilage regeneration and bone formation promotion; 350-500KDa are used in pharmaceutical treatment to achieve tissue regeneration, corneal regeneration and treatment of musculoskeletal diseases; 40-60KDa are used in cosmetics and foods to achieve barrier repair, wrinkle improvement and skin beauty. Based on this, in the process of PDRN preparation, it is of great significance to prepare PDRN with different molecular weights according to different applications.

[0004] It is human nature to love beauty. The accelerated pace of life, long-term work pressure and mental tension will have adverse effects on people's skin. Therefore, skin beauty has attracted more and more attention, especially facial skin beauty.

[0005] Jiang Haiyan et al. (Clinical Observation on the Improvement of Facial Pores by Polydeoxyribonucleic Acid [J], Chinese Medical Cosmetology, Vol. 10, No. 3 (Total No. 79), March 2020) found that 0.5% PDRN treatment can improve the enlarged pores on the superficial surface of the skin. Wang Mengchun et al. (The Application Effect of Fractional Laser Combined with Polydeoxyribonucleic Acid in Facial Skin Cosmetology [J], Chinese Cosmetic Medicine, Vol. 32, No. 12, December 2023) found that immediately applying PDRN after fractional laser treatment can be used for facial skin cosmetic treatment, which has significant effects and helps improve the skin microcirculation of patients, and is worthy of clinical promotion.

[0006] CN1155006308A discloses an anti-aging and delicate anti-aging essence, comprising the following components in percentage by mass: 3-10% of Chondrus crispus extract; 3-5% of oat kernel oil; 1-5% of baobab seed oil; 0.05-0.2% of DNA sodium; 0.1-1% of collagen; 0.1-1% of calendula flower extract; 1-10% of medicinal pore fungus extract; 2-6% of betaine; 0.55-1.15% of composite sclerotium gum; 1-5% of composite polypeptide; 1-4% of emulsifier; 3.4-25% of emollient; 0.1-1% of preservative; and the balance is solvent. The invention has the advantages of being able to resist both endogenous and exogenous aging, so that the skin can have a certain sunscreen and anti-aging effect without the need for additional sunscreen.

[0007] In recent years, the application areas of PDRN have expanded to medical beauty and skin care. It is extremely challenging to provide a DNA sodium raw material that is more suitable for skin beauty by combining the PDRN preparation method. Summary of the invention

[0008] The object of the present invention is to provide a composition containing sodium hyaluronate and sodium DNA and its application. The sodium DNA in the composition is prepared by enzymatic hydrolysis technology, has a molecular weight of 30-50 kDa, and has better anti-aging effect than the prior art.

[0009] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0010] In a first aspect, the present invention provides a composition containing sodium hyaluronate and sodium DNA, comprising the following raw materials: sodium DNA, sodium hyaluronate and mannitol.

[0011] In some embodiments, the method for preparing sodium DNA comprises the following steps:

[0012] (1) adding alkali solution to the fish tissue and grinding it to make a slurry;

[0013] (2) adding enzyme solution to the slurry for enzymolysis to obtain an enzymolysis solution;

[0014] (3) adding HCl to the enzymatic hydrolyzate, supernatant, centrifuging, and taking the supernatant;

[0015] (4) ultrafiltration of the supernatant, and drying of the filtrate to obtain the DNA sodium.

[0016] Preferably, the fish tissue in step (1) is at least one of the ovary, testis, semen and egg of fish; and the fish is at least one of trout, salmon and salmon.

[0017] Preferably, the alkali solution in step (1) is 1-4 mol / L NaOH; more preferably, it is 2-3 mol / L NaOH.

[0018] Preferably, the mass ratio of the alkali solution to the fish tissue in step (1) is 3-10:1; more preferably 5-8:1.

[0019] Preferably, the grinding in step (1) is: grinding at 3000-5000 rpm for 5-10 min.

[0020] Preferably, the amount of the enzyme solution added in step (2) is 0.2-0.5% of the mass of the slurry; more preferably, it is 0.3-0.4%.

[0021] Preferably, the enzyme solution in step (2) is composed of 80-120 mM NaCl and 10-20 mg / mL composite protease, wherein the composite protease is alkaline protease, ficin and proteinase K in a mass ratio of 1-7:1-5:1.

[0022] Further preferably, the enzyme solution consists of 90-110 mM NaCl and 12-18 mg / mL composite protease.

[0023] Further preferably, the mass ratio of alkaline protease, ficin and proteinase K is 3-5:3-5:1.

[0024] Preferably, the conditions for the enzymatic hydrolysis in step (2) are: pH 7.5-8.0, temperature 35-40° C., and enzymatic hydrolysis time 1-5 h; more preferably 2-4 h.

[0025] Preferably, the concentration of HCl in step (3) is 1-3 mol / L; more preferably, 2-3 mol / L.

[0026] Preferably, the amount of HCl added in step (3) is 30-100% of the volume of the enzymatic hydrolyzate; more preferably, it is 50-80%.

[0027] Preferably, the ultrasound in step (3) is: ultrasound at 500-1000W for 5-10 min, more preferably ultrasound at 800W for 7 min.

[0028] Preferably, the ultrafiltration membrane used in the ultrafiltration in step (4) is 30-50 kDa.

[0029] Preferably, the drying in step (4) is spray drying, vacuum drying or freeze drying; more preferably freeze drying or spray drying.

[0030] In some embodiments, the mass ratio of sodium DNA, sodium hyaluronate and mannitol in the composition is 1:5-10:1-7; more preferably 1:7-9:3-5; and even more preferably 1:8:4.

[0031] In a second aspect, the present invention provides a method for preparing the above-mentioned composition containing sodium hyaluronate and sodium DNA: sodium DNA, sodium hyaluronate and mannitol are mixed with water.

[0032] In some embodiments, the amount of water added is 70-90 times the mass of DNA sodium; preferably 75-85 times.

[0033] In a third aspect, the present invention provides a method for improving the stability of sodium DNA, comprising the following steps: mixing sodium DNA, sodium hyaluronate and mannitol with water.

[0034] In some embodiments, the mass ratio of sodium DNA, sodium hyaluronate and mannitol is 1:5-10:1-7; more preferably 1:7-9:3-5; and even more preferably 1:8:4.

[0035] In a fourth aspect, the present invention provides the use of the above-mentioned composition containing sodium hyaluronate and sodium DNA in the preparation of cosmetic products.

[0036] In a fifth aspect, the present invention provides a cosmetic product comprising the above-mentioned composition containing sodium hyaluronate and sodium DNA.

[0037] The beauty product is food, skin care product or medical beauty product.

[0038] The technical effects achieved by the present invention are:

[0039] (1) The DNA sodium of the present invention is prepared by enzymatic hydrolysis technology, has a molecular weight of 30-50 kDa, and contains endotoxin less than 0.5 EU / mg. Compared with the prior art, the DNA sodium of the present invention is safer.

[0040] (2) The preparation method of DNA sodium of the present invention is simple, easy to operate, has good reproducibility, and is suitable for large-scale promotion and application.

[0041] (3) The composition of the present invention comprises sodium DNA, sodium hyaluronate and mannitol. The combination of sodium hyaluronate, mannitol and sodium DNA can improve the stability of sodium DNA in liquid state on the one hand, and greatly enhance the anti-aging effect of sodium DNA on the other hand, thereby providing technical support for the application of sodium DNA in the field of medical beauty. DETAILED DESCRIPTION

[0042] The following describes the embodiments of the present invention through specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present invention.

[0043] Before further describing the specific embodiments of the present invention, it should be understood that the scope of protection of the present invention is not limited to the specific embodiments described below; it should also be understood that the terms used in the examples of the present invention are for describing specific embodiments rather than for limiting the scope of protection of the present invention.

[0044] When the embodiment gives a numerical range, it should be understood that, unless otherwise specified in the present invention, the two endpoints of each numerical range and any numerical value between the two endpoints can be selected. Unless otherwise defined, all technical and scientific terms used herein have the same meanings as those of ordinary skill in the art to which the present invention belongs.

[0045] It is worth noting that the raw materials used in the present invention are all common commercial products, and therefore their sources are not specifically limited. The molecular weight of hyaluronic acid is 50kDa. 500,000 U / g of alkaline protease, 400,000 U / g of neutral protease, 800,000 U / g of papain, 500,000 U / g of bromelain are all purchased from Nanning Dongheng Huadao Biotechnology Co., Ltd., 500,000 U / g of ficin, purchased from Shaanxi Chenming Biotechnology Co., Ltd., 400,000 U / mg of proteinase K, purchased from Shandong Si Ke Jie Biotechnology Co., Ltd.

[0046] Example 1 Composition containing sodium hyaluronate and sodium DNA

[0047] The raw materials of the composition are: 1 part of sodium DNA, 8 parts of sodium hyaluronate, 4 parts of mannitol and 80 parts of water by weight.

[0048] The preparation method of the DNA sodium is:

[0049] (1) Add 6 g of alkaline solution (2 mol / L NaOH) to 1 g of trout ovary and grind at 4000 rpm for 7 min to prepare a slurry;

[0050] (2) adding 21 mg of enzyme solution (0.3% of the mass of the slurry) to the slurry, performing enzymolysis at pH 7.5-8.0 and temperature 37±2°C for 3 h to obtain an enzymolysis solution;

[0051] The enzyme solution is composed of 100 mM NaCl and 15 mg / mL composite protease, wherein the composite protease is alkaline protease, ficin and proteinase K in a mass ratio of 4:3:1.

[0052] (3) Add 4.2 g 2 mol / L HCl to the enzymatic hydrolyzate, sonicate at 800 W for 7 min, centrifuge at 3000 rpm for 5 min, and collect the supernatant;

[0053] (4) The supernatant is filtered through a 40 kDa ultrafiltration, and the filtrate is spray-dried at 40° C. to obtain the DNA sodium.

[0054] The preparation method of the composition is: stir the formulated amounts of sodium DNA, sodium hyaluronate, mannitol and water until they are evenly mixed.

[0055] Example 2 Composition containing sodium hyaluronate and sodium DNA

[0056] The raw materials of the composition are: 1 part of sodium DNA, 5 parts of sodium hyaluronate, 1 part of mannitol and 70 parts of water by weight.

[0057] The preparation method of the DNA sodium is the same as that of Example 1.

[0058] The preparation method of the composition is the same as that of Example 1.

[0059] Example 3 Composition containing sodium hyaluronate and sodium DNA

[0060] The raw materials of the composition are: 1 part of sodium DNA, 10 parts of sodium hyaluronate, 7 parts of mannitol and 90 parts of water by weight.

[0061] The preparation method of the DNA sodium is the same as that of Example 1.

[0062] The preparation method of the composition is the same as that of Example 1.

[0063] Example 4 Composition containing sodium hyaluronate and sodium DNA

[0064] The difference between this embodiment and embodiment 1 is only that the preparation method of the DNA sodium is different.

[0065] Specifically, the preparation method of the DNA sodium is:

[0066] (1) Add 10 g of alkaline solution (1 mol / L NaOH) to 1 g of trout ovary and grind at 4000 rpm for 7 min to prepare a slurry;

[0067] (2) adding 55 mg of enzyme solution (0.5% of the mass of the slurry) to the slurry, performing enzymolysis at pH 7.5-8.0 and temperature 37±2°C for 2 h to obtain an enzymolysis solution;

[0068] The enzyme solution is composed of 80 mM NaCl and 20 mg / mL composite protease, wherein the composite protease is alkaline protease, ficin and proteinase K in a mass ratio of 1:1:1.

[0069] (3) Add 4.2 g 2 mol / L HCl to the enzymatic hydrolyzate, sonicate at 800 W for 7 min, centrifuge at 3000 rpm for 5 min, and collect the supernatant;

[0070] (4) The supernatant is filtered through a 40 kDa ultrafiltration, and the filtrate is spray-dried at 40° C. to obtain the DNA sodium.

[0071] Example 5 Composition containing sodium hyaluronate and sodium DNA

[0072] The difference between this embodiment and embodiment 1 is only that the preparation method of the DNA sodium is different.

[0073] Specifically, the preparation method of the DNA sodium is:

[0074] (1) Add 3 g of alkaline solution (4 mol / L NaOH) to 1 g of trout ovary and grind at 4000 rpm for 7 min to prepare a slurry;

[0075] (2) adding 8 mg of enzyme solution (0.2% of the mass of the slurry) to the slurry, performing enzymolysis at pH 7.5-8.0 and temperature 37±2°C for 4 h to obtain an enzymolysis solution;

[0076] The enzyme solution is composed of 120 mM NaCl and 20 mg / mL composite protease, wherein the composite protease is alkaline protease, ficin and proteinase K in a mass ratio of 7:5:1.

[0077] (3) Add 4.2 g 2 mol / L HCl to the enzymatic hydrolyzate, sonicate at 800 W for 7 min, centrifuge at 3000 rpm for 5 min, and collect the supernatant;

[0078] (4) The supernatant is filtered through a 40 kDa ultrafiltration, and the filtrate is spray-dried at 40° C. to obtain the DNA sodium.

[0079] Comparative Example 1 Composition containing sodium hyaluronate and sodium DNA

[0080] The difference between this comparative example and Example 1 is only that the preparation method of DNA sodium is different (the enzyme solution is different and does not contain sodium chloride).

[0081] Specifically, the preparation method of the DNA sodium is:

[0082] (1) Add 6 g of alkaline solution (2 mol / L NaOH) to 1 g of trout ovary and grind at 4000 rpm for 7 min to prepare a slurry;

[0083] (2) adding 21 mg of enzyme solution (0.3% of the mass of the slurry) to the slurry, performing enzymolysis at pH 7.5-8.0 and temperature 37±2°C for 3 h to obtain an enzymolysis solution;

[0084] The enzyme solution is 15 mg / mL of a composite protease, wherein the composite protease is alkaline protease, ficin and proteinase K in a mass ratio of 4:3:1.

[0085] (3) Add 4.2 g 2 mol / L HCl to the enzymatic hydrolyzate, sonicate at 800 W for 7 min, centrifuge at 3000 rpm for 5 min, and collect the supernatant;

[0086] (4) The supernatant is filtered through a 40 kDa ultrafiltration, and the filtrate is spray-dried at 40° C. to obtain the DNA sodium.

[0087] Comparative Example 2 Composition containing sodium hyaluronate and sodium DNA

[0088] The difference between this comparative example and Example 1 is only that the preparation method of DNA sodium is different (different composite proteases).

[0089] Specifically, the preparation method of the DNA sodium is:

[0090] (1) Add 6 g of alkaline solution (2 mol / L NaOH) to 1 g of trout ovary and grind at 4000 rpm for 7 min to prepare a slurry;

[0091] (2) adding 21 mg of enzyme solution (0.3% of the mass of the slurry) to the slurry, performing enzymolysis at pH 7.5-8.0 and temperature 37±2°C for 3 h to obtain an enzymolysis solution;

[0092] The enzyme solution is composed of 100 mM NaCl and 15 mg / mL composite protease, wherein the composite protease is alkaline protease, bromelain and proteinase K in a mass ratio of 4:3:1.

[0093] (3) Add 4.2 g 2 mol / L HCl to the enzymatic hydrolyzate, sonicate at 800 W for 7 min, centrifuge at 3000 rpm for 5 min, and collect the supernatant;

[0094] (4) The supernatant is filtered through a 40 kDa ultrafiltration, and the filtrate is spray-dried at 40° C. to obtain the DNA sodium.

[0095] Comparative Example 3 Composition containing sodium hyaluronate and sodium DNA

[0096] The difference between this comparative example and Example 1 is only that the preparation method of DNA sodium is different (different composite proteases).

[0097] Specifically, the preparation method of the DNA sodium is:

[0098] (1) Add 6 g of alkaline solution (2 mol / L NaOH) to 1 g of trout ovary and grind at 4000 rpm for 7 min to prepare a slurry;

[0099] (2) adding 21 mg of enzyme solution (0.3% of the mass of the slurry) to the slurry, performing enzymolysis at pH 7.5-8.0 and temperature 37±2°C for 3 h to obtain an enzymolysis solution;

[0100] The enzyme solution is composed of 100 mM NaCl and 15 mg / mL composite protease, wherein the composite protease is alkaline protease, elastase and papain in a mass ratio of 4:3:1.

[0101] (3) Add 4.2 g 2 mol / L HCl to the enzymatic hydrolyzate, sonicate at 800 W for 7 min, centrifuge at 3000 rpm for 5 min, and collect the supernatant;

[0102] (4) The supernatant is filtered through a 40 kDa ultrafiltration, and the filtrate is spray-dried at 40° C. to obtain the DNA sodium.

[0103] Comparative Example 4 Composition containing sodium hyaluronate and sodium DNA

[0104] The difference between this comparative example and Example 1 is only that the preparation method of DNA sodium is different (different composite proteases).

[0105] Specifically, the preparation method of the DNA sodium is:

[0106] (1) Add 6 g of alkaline solution (2 mol / L NaOH) to 1 g of trout ovary and grind at 4000 rpm for 7 min to prepare a slurry;

[0107] (2) adding 21 mg of enzyme solution (0.3% of the mass of the slurry) to the slurry, performing enzymolysis at pH 7.5-8.0 and temperature 37±2°C for 3 h to obtain an enzymolysis solution;

[0108] The enzyme solution is composed of 100 mM NaCl and 15 mg / mL composite protease, wherein the composite protease is alkaline protease, ficin and proteinase K in a mass ratio of 5:1:7.

[0109] (3) Add 4.2 g 2 mol / L HCl to the enzymatic hydrolyzate, sonicate at 800 W for 7 min, centrifuge at 3000 rpm for 5 min, and collect the supernatant;

[0110] (4) The supernatant is filtered through a 40 kDa ultrafiltration, and the filtrate is spray-dried at 40° C. to obtain the DNA sodium.

[0111] 1. Endotoxin detection

[0112] Refer to Appendix XI E of the Chinese Pharmacopoeia (Part II) for bacterial endotoxin detection. Endotoxin detection was performed on the DNA sodium prepared in the examples and comparative examples, and the results are shown in Table 1.

[0113] Table 1

[0114]

[0115]

[0116] The results showed that the sodium endotoxin content of the DNA prepared by the present invention was lower than 0.5EU / mg, and had good safety.

[0117] 2. Anti-aging evaluation

[0118] Test sample: the composition containing sodium hyaluronate and sodium DNA prepared in Examples 1-5 and Comparative Examples 1-4.

[0119] Experimental method: After adaptive feeding for 3 days, KM mice (22-26g, male) were depilated on the back to expose 2cm×2cm of skin. The depilated mice were randomly divided into a blank control group, a model group, an embodiment 1-5 group and a comparative example 1-4 group according to body weight, with 8 mice in each group. Except for the blank control group, the other groups were subcutaneously injected with 1000mg / kg of D-galactose on the back every day, and the blank control group was injected with 0.2ml of normal saline every day for 6 consecutive weeks to construct an acute aging mouse model. After the 5th week, the composition prepared in the corresponding embodiments and comparative examples (dose 250mg / kg) was applied to the back of the mice in the embodiment 1-5 group and the comparative example 1-4 group, and 0.2ml of water was applied to the blank control group. The drug was administered once a day for 4 consecutive weeks. During the experiment, each group of mice was depilated once a week.

[0120] Index detection: After the last administration, the mice in each group fasted for 24 hours, then were killed, the back skin was cut, the subcutaneous tissue was removed, and the skin tissue was obtained by rinsing with pre-cooled saline. The excess water was absorbed and the skin tissue was obtained. According to the mass to volume ratio of 1g:9mL, saline was added to the skin tissue, the back skin was cut into pieces to make a homogenate, centrifuged at 3500r / min for 20min, and the supernatant was collected. The contents of SOD, GSH-Px and MDA in the skin tissue homogenate were detected using a kit.

[0121] The results are shown in Table 2.

[0122] Table 2

[0123]

[0124]

[0125] Note: Different letters in the same column indicate significant differences among the groups (P < 0.05).

[0126] The results showed that compared with the normal control group, the SOD content in the skin tissue of the mice in the model group was significantly decreased (P < 0.05), and the MDA content was significantly increased (P < 0.05), indicating that the model was successfully established.

[0127] Compared with the model group, the SOD content in the skin tissue of the mice in the Examples 1-5 groups was significantly increased (P < 0.05), and the MDA content was significantly decreased (P < 0.05), indicating that the composition prepared in Examples 1-5 can improve the antioxidant capacity of the skin, reduce the generation of peroxidation products, and has a good anti-aging effect.

[0128] By comparing Example 1 and Comparative Examples 1-4, it can be seen that compared with Example 1, the changes in SOD and MDA contents in the skin tissue of mice in Comparative Examples 1-4 groups were significantly different (P < 0.05), indicating that changing the preparation method of DNA sodium has an important impact on the anti-aging effect of DNA sodium.

[0129] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions of the technical solution of the present invention by ordinary technicians in this field do not deviate from the essence and scope of the technical solution of the present invention.

Claims

1. A composition containing sodium hyaluronate and sodium DNA, characterized in that: The raw materials include: sodium DNA, sodium hyaluronate and mannitol; The preparation method of DNA sodium comprises the following steps: (1) adding alkali solution to the fish tissue and grinding it to make a slurry; (2) adding an enzyme solution to the slurry for enzymolysis to obtain an enzymolysis solution; (3) adding HCl to the enzymatic hydrolyzate, supernatant, centrifuging, and taking the supernatant; (4) ultrafiltration of the supernatant, and drying of the filtrate to obtain the DNA sodium.

2. The composition according to claim 1, characterized in that The amount of enzyme solution added in step (2) is 0.2-0.5% of the mass of the slurry; and / or The enzyme solution in step (2) is composed of 80-120 mM NaCl and 10-20 mg / mL composite protease, wherein the composite protease is alkaline protease, ficin and proteinase K in a mass ratio of 1-7:1-5:1; and / or The conditions for the enzymatic hydrolysis in step (2) are: pH 7.5-8.0, temperature 35-40° C., and enzymatic hydrolysis time 1-5 h.

3. The composition according to claim 1, characterized in that The concentration of HCl in step (3) is 1-3 mol / L; and / or The amount of HCl added in step (3) is 30-100% of the volume of the enzymatic hydrolyzate; and / or The ultrasound in step (3) is: ultrasound at 500-1000W for 5-10min.

4. The composition according to claim 1, characterized in that The ultrafiltration membrane used in the ultrafiltration in step (4) is 30-50 kDa.

5. The composition according to any one of claims 1 to 4, characterized in that The mass ratio of sodium DNA, sodium hyaluronate and mannitol in the composition is 1:5-10:1-7.

6. A method for preparing the composition according to any one of claims 1 to 4, characterized in that: The method comprises the following steps: adding water to mix sodium DNA, sodium hyaluronate and mannitol.

7. A method for improving the stability of sodium DNA, comprising the following steps: mixing sodium DNA, sodium hyaluronate and mannitol with water; The mass ratio of the sodium DNA, sodium hyaluronate and mannitol is 1:5-10:1-7.

8. Use of the composition according to any one of claims 1 to 4 in the preparation of cosmetic products.

9. A beauty product, characterized in that: A composition comprising any one of claims 1 to 4.

10. The cosmetic product according to claim 9, characterized in that: The beauty product is food, skin care product or medical beauty product.

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