Method for restoring heavy metal pollution of riverway through aquatic plant composite planting

By planting floating-leaved and submerged plants in different areas of the river channel, and combining them with microbial agents and biological growth promoters, the problem of high cost and unsatisfactory results in the treatment of heavy metal polluted water has been solved, achieving low-cost and high-efficiency water purification.

CN119285112AActive Publication Date: 2025-01-10NORTH CHINA UNIV OF WATER RESOURCES & ELECTRIC POWER +1
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Patent Information

Application Number
CN202411245530.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-09-06
Publication Date
2025-01-10
Estimated Expiration
2044-09-06

AI Technical Summary

Technical Problem

In the treatment of heavy metal polluted water, existing technologies, such as physical and chemical remediation methods, require huge investments and have unsatisfactory results, while the remediation effect of simply using aquatic plants is generally average, and there is a lack of efficient and low-cost remediation solutions.

Method used

Aquatic plant intercropping method is adopted, which combines microbial agents and biological growth promoters. Floating-leaved plants and submerged plants are planted in different areas of the river channel, and microbial agents such as EM bacteria, Bacillus subtilis, Bacillus cereus, Rhodopseudomonas spheroidae, and Actinomycetes are used in combination with biological growth promoters to promote plant growth and the absorption and transformation of heavy metals.

Benefits of technology

It enhances the ability of aquatic plants to remediate heavy metal pollution, improves water quality, reduces the concentration of harmful substances, and improves the water purification effect, thus achieving low-cost remediation of heavy metal pollution.

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Abstract

The invention relates to the technical field of ecological restoration. The invention provides a method for restoring heavy metal pollution of a riverway through aquatic plant composite planting. The method comprises the following steps: (1) spreading a microbial agent into a riverway water body to be restored for preliminary restoration; and (2) arranging a floating bed and planting floating-leaved plants in a midstream area of the preliminarily restored river water body, planting submerged plants in a downstream area, and then spreading a biological growth promoting agent. According to the technical scheme, different aquatic plants are planted in different areas, so that the adsorption effect of the corresponding aquatic plants on the heavy metals in the river channel is better exerted; the combined use of the microbial agent and the biological growth promoting agent not only can promote the growth of aquatic plants and beneficial microorganisms, but also can improve the absorption, transformation and degradation effects on heavy metals, enhance the remediation capability of plants on heavy metal pollution, and achieve the purpose of fully purifying water.
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Description

Technical Field

[0001] The invention relates to the technical field of ecological restoration, and in particular to a method for restoring heavy metal pollution in a river by composite planting of aquatic plants. Background Art

[0003] The main methods for the treatment and restoration of heavy metal polluted water bodies include chemical, physical, and biological methods. Physical and chemical restoration not only require huge investments but also have less than ideal results. In comparison, the biological method of using aquatic plants for ecological restoration has the advantages of low treatment costs, environmental protection, and beautiful appearance. However, the restoration effect of aquatic plants alone is also average.

[0004] Therefore, it is very necessary to study and obtain a remediation method with low investment cost and good heavy metal pollution remediation effect. Summary of the invention

[0005] The purpose of the present invention is to provide a method for repairing heavy metal pollution in rivers by composite planting of aquatic plants. By combining different aquatic plants, microbial agents and biological growth promoters, it can not only promote the growth of aquatic plants and beneficial microorganisms, but also improve the absorption, transformation and degradation of heavy metals, enhance the plant's ability to repair heavy metal pollution, and achieve the purpose of fully purifying the water body.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0007] The present invention provides a method for repairing heavy metal pollution in a river by composite planting of aquatic plants, comprising the following steps:

[0008] (1) Spreading microbial agents into the water body of the river to be repaired for preliminary repair;

[0009] (2) Set up floating beds and plant floating-leaf plants in the midstream area of ​​the river after preliminary restoration, plant submerged plants in the downstream area, and then spread biological growth promoters.

[0010] Preferably, the microbial agent in step (1) includes any three or more of EM bacteria, Bacillus subtilis, Bacillus cereus, Rhodopseudomonas sphaeroides, and actinomycetes.

[0011] Preferably, when the microbial agent is EM bacteria, Bacillus subtilis, Bacillus cereus, Rhodopseudomonas sphaeroides and actinomycetes, the mass ratio of EM bacteria, Bacillus subtilis, Bacillus cereus, Rhodopseudomonas sphaeroides and actinomycetes is (0.8-1.2):(9-13):(6-8):(8-12):(10-15).

[0012] Preferably, the effective viable count of each bacterium in the microbial agent is (5-15)×107 cfu / mL; the application amount of the microbial agent is 1-3mL / L.

[0013] Preferably, the floating-leaf plants in step (2) include any two or more of Nymphoides, Algae, Water Chestnut, and Eichhornia crassipes; and the submerged plants include any two or more of Potamogeton crispus, Ceratophyllum, Chara, and Hydrilla.

[0014] Preferably, the planting density of the floating leaf plants in step (2) is 20-30 plants / m 2 The planting area is 15-25% of the total area of ​​the midstream area; the planting density of the submerged plants is 30-40 plants / m 2 , the planting area accounts for 20-30% of the total area in the downstream area.

[0015] Preferably, the biological growth promoter in step (2) comprises the following raw materials in percentage by weight: 0.8-1.2% trace elements, 1.5-2% amino acids, 6-7% complex vitamins, 2-4% growth-promoting ingredients, 4-6% biological enzymes, 1-2% water-soluble polymers and the remainder water.

[0016] Preferably, the trace elements include any one or more of iron, zinc or magnesium.

[0017] Preferably, the amino acid comprises L-cysteine.

[0018] Preferably, the vitamin complex includes vitamin B and / or biotin.

[0019] Preferably, the growth-promoting ingredients include any one or more of auxin, cytokinin or gibberellin.

[0020] Preferably, the biological enzyme comprises protease and / or cellulase.

[0021] Preferably, the water-soluble polymer includes hypromellose or hydroxypropyl cellulose.

[0022] Preferably, the application amount of the biopromoter is 20-40 μL / L.

[0023] By adopting the above technical solution, the present invention has the following beneficial effects:

[0024] 1. In the technical solution of the present invention, the midstream area and the downstream area are divided to plant floating-leaf plants and submerged plants respectively, so as to better exert the adsorption effect of the corresponding aquatic plants on heavy metals in the river.

[0025] 2. The microbial agent used in the technical solution of the present invention is a composite agent, including EM bacteria, Bacillus subtilis, Bacillus cereus, actinomycetes, etc., which can decompose organic matter and transform harmful substances into harmless substances through their metabolites and active substances, and promote self-purification in water bodies to improve river water quality and repair heavy metal pollution. The microbial agent and the biological growth promoter in the present invention are used in combination, which can not only act on microorganisms that are beneficial to heavy metal repair, but also synergistically act on floating leaf plants and submerged plants with heavy metal adsorption, improve the absorption, transformation and degradation of heavy metals, enhance the ability of aquatic plants to repair heavy metal pollution, and achieve the purpose of fully purifying the water body. DETAILED DESCRIPTION

[0026] The present invention provides a method for repairing heavy metal pollution in a river by composite planting of aquatic plants, comprising the following steps:

[0027] (1) Spreading microbial agents into the water body of the river to be repaired for preliminary repair;

[0028] (2) Set up floating beds and plant floating-leaf plants in the midstream area of ​​the river after preliminary restoration, plant submerged plants in the downstream area, and then spread biological growth promoters.

[0029] In the present invention, microbial agents are spread into the water body of the river to be repaired for preliminary repair, and the microbial agents include any three or more of EM bacteria, Bacillus subtilis, Bacillus cereus, Rhodopseudomonas sphaeroides, and actinomycetes. The microbial agents described in the present invention improve the water quality of the river and repair heavy metal pollution through different mechanisms of action, such as water quality improvement, sediment repair, heavy metal passivation, etc.; they can also decompose organic matter and transform harmful substances into harmless substances through their metabolites and active substances, while promoting self-purification in the water body. Among them, EM bacteria can decompose harmful substances and odors in water through metabolism, improve the transparency and oxygen content of water quality, reduce the concentration of harmful substances such as ammonia nitrogen and nitrite, and at the same time increase the number of beneficial bacteria to improve water quality; they can also promote the growth and reproduction of aquatic plants and increase the stability of the ecosystem. The Bacillus subtilis and Bacillus cereus have strong environmental adaptability and stress resistance, and can survive and reproduce under various environmental conditions; they can effectively absorb and accumulate heavy metals through biological dissolution and precipitation, biological adsorption and enrichment, and biological transformation, thereby reducing their mobility and bioavailability in the environment, and achieving the purpose of repairing heavy metal pollution. The spherical Rhodopseudomonas belongs to photosynthetic bacteria, which can use light as energy and fish and shrimp residues and excrement in water as carbon sources under anaerobic conditions to carry out non-oxygen photosynthesis and synthesize a large number of bacteria; it can effectively absorb and transform harmful substances such as ammonia nitrogen, nitrite, and hydrogen sulfide, which can increase dissolved oxygen in water and reduce substances harmful to aquatic animals, and can also adjust the pH value of water bodies within a suitable range, and has good water quality control capabilities. The actinomycetes can use amino acids, ammonia, etc. as substrates to produce various antibiotics and biological enzymes to inhibit the growth and proliferation of other harmful microorganisms; and the actinomycetes can also compete for the substrates required for the proliferation of harmful microorganisms, thereby inhibiting their proliferation and growth. The above-mentioned several microorganisms are used in a coordinated manner to better enhance the effect of repairing heavy metal pollution.

[0030] In the present invention, when the microbial agent is EM bacteria, Bacillus subtilis, Bacillus cereus, Rhodopseudomonas sphaeroides and actinomycetes, the mass ratio of EM bacteria, Bacillus subtilis, Bacillus cereus, Rhodopseudomonas sphaeroides and actinomycetes is preferably (0.8-1.2):(9-13):(6-8):(8-12):(10-15), further preferably (0.9-1.1):(9.5-12):(6.5-7.5):(9-11.5):(10.5-14), and further preferably 1:10.5:8:10:12.5.

[0031] In the present invention, the effective viable count of each bacterium in the microbial agent is preferably (5-15)×10 7 cfu / mL, and more preferably (8-14)×10 7cfu / mL, and more preferably 12×10 7 cfu / mL; the application amount of the microbial agent is preferably 1-3mL / L, further preferably 1.2-2.5mL / L, and further preferably 2mL / L.

[0032] In the present invention, a floating bed is set in the midstream area of ​​the river water body after preliminary repair and floating leaf plants are planted. In the present invention, floating leaf plants are planted in the upper half of the floating bed exposed to the water surface, and the floating bed is preferably a "well" shaped structure, which has strong stability and can effectively prevent the floating bed from drifting under the action of water flow; at the same time, the "well" shaped structure can also increase the contact area between the floating bed and the water body, and better improve the purification effect. In the present invention, the floating leaf plants include any two or more of water chestnuts, fine green duckweed, water chestnuts, and water hyacinths. When the floating leaf plants in the present invention are preferably water chestnuts, fine green duckweed and water hyacinths, the number ratio of water chestnuts, fine green duckweed and water hyacinths is preferably (1-2): (3-5): 1, further preferably (1.2-1.8): (3.5-4.6): 1, and further preferably 1.5: 4: 1.

[0033] In the present invention, the planting density of the floating leaf plants is preferably 20-30 plants / m 2 , further preferably 22-28 plants / m 2 , and further preferably 26 plants / m 2 The planting area is preferably 15-25% of the total area of ​​the midstream region, further preferably 18-22%, and further preferably 20%.

[0034] In the present invention, submerged plants are planted in the downstream area of ​​the river water body after preliminary repair, and the submerged plants include any two or more of water chestnut, hornwort, chara, and black algae. When the submerged plants in the present invention are preferably water chestnut, hornwort, and chara, the quantity ratio of water chestnut, hornwort, and chara is preferably (2-4): (1-3): (1-2), further preferably (2.4-3.5): (1.5-2.5): (1.2-1.8), and further preferably 3:2:1.5.

[0035] In the present invention, the planting density of the submerged plants is preferably 30-40 plants / m 2 , and further preferably 32-38 plants / m 2 , and further preferably 36 plants / m 2 The planting area is preferably 20-30% of the total area of ​​the downstream region, further preferably 22-28%, and further preferably 25%.

[0036] In the present invention, after the aquatic plants are planted, the biological growth promoter is spread to the middle and downstream planting areas. The biological growth promoter in the present invention includes the following raw materials: trace elements, amino acids, complex vitamins, growth-promoting ingredients, biological enzymes, water-soluble polymers and water.

[0037] In the present invention, the trace elements include any one or more of iron, zinc or magnesium, and more preferably iron, zinc and magnesium. The amino acids described in the present invention include L-cysteine. The vitamin complex described in the present invention preferably includes vitamin B and / or biotin, and more preferably vitamin B and biotin. The growth-promoting ingredients described in the present invention preferably include any one or more of auxin, cytokinin or gibberellin, and more preferably include auxin, cytokinin and gibberellin. The biological enzymes described in the present invention preferably include proteases and / or cellulases, and more preferably include proteases and cellulases. The water-soluble polymers described in the present invention preferably include hydroxypropyl methylcellulose or hydroxypropyl cellulose, and more preferably include hydroxypropyl methylcellulose.

[0038] In the present invention, the biological growth promoter preferably includes the following raw materials in mass percentage: 0.8-1.2% trace elements, 1.5-2% amino acids, 6-7% complex vitamins, 2-4% growth-promoting ingredients, 4-6% biological enzymes, 1-2% water-soluble polymers and the remainder water; further preferably includes 0.9-1.1% trace elements, 1.6-1.9% amino acids, 6.2-6.8% complex vitamins, 2.3-3.6% growth-promoting ingredients, 4.2-5.5% biological enzymes, 1.2-1.8% water-soluble polymers and the remainder water, and further preferably includes 1% trace elements, 1.8% amino acids, 6.5% complex vitamins, 3.2% growth-promoting ingredients, 5% biological enzymes, 1.5% water-soluble polymers and the remainder water.

[0039] In the present invention, the application amount of the biopromoter is preferably 20-40 μL / L, further preferably 24-36 μL / L, and further preferably 32 μL / L.

[0040] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0041] The EM bacteria, Bacillus subtilis, Bacillus cereus, Rhodopseudomonas sphaeroides and actinomycetes described in the present invention are all purchased from Protin Biotechnology (Beijing) Co., Ltd.

[0042] Example 1

[0043] A method for repairing heavy metal pollution in a river by composite planting of aquatic plants, comprising the following steps:

[0044] (1) A microbial agent was applied to the water body of the river to be repaired at an application rate of 2 mL / L for preliminary repair. The microbial agent was EM bacteria, Bacillus subtilis, Bacillus cereus, Rhodopseudomonas sphaeroides and actinomycetes in a mass ratio of 1:10.5:8:10:12.5, and the effective viable count of each bacterium in the microbial agent was 12×10 7 cfu / mL;

[0045] (2) Floating beds were set up in the middle reaches of the river after preliminary restoration, and Nymphoides, Algae serrata, and Eichhornia crassipes were planted in a ratio of 1.5:4:1, with a planting density of 26 plants / m 2 , the planting area is 20% of the total area in the midstream region;

[0046] (3) In the downstream area of ​​the river after preliminary restoration, the ratio of water chestnut, hornwort and charophyte was 3:2:1.5, and the planting density was 36 plants / m 2 , the planting area is 25% of the total area in the downstream area;

[0047] (4) After planting the aquatic plants, a biological growth promoter is applied at a dosage of 32 μL / L. The biological growth promoter is composed of the following raw materials in percentage by mass: 0.3% iron, 0.3% zinc, 0.4% magnesium, 1.8% L-cysteine, 4% vitamin B, 2.5% biotin, 1% auxin, 1% cytokinin, 1.2% gibberellin, 2% protease, 3% cellulase, 1.5% hydroxypropyl methylcellulose and the remainder water.

[0048] Example 2

[0049] A method for repairing heavy metal pollution in a river by composite planting of aquatic plants, comprising the following steps:

[0050] (1) A microbial agent was applied to the water body of the river to be repaired at an application rate of 3 mL / L for preliminary repair. The microbial agent was EM bacteria, Bacillus subtilis, Bacillus cereus, Rhodopseudomonas sphaeroides and actinomycetes in a mass ratio of 0.8:9:6:12:15, and the effective viable count of each bacterium in the microbial agent was 5×10 7 cfu / mL;

[0051] (2) Floating beds were set up in the middle reaches of the river after the initial restoration, and Nymphoides, Algae, and Water Hyacinthus were planted in a ratio of 1:5:1, with a planting density of 20 plants / m 2 , the planting area is 15% of the total area in the midstream region;

[0052] (3) In the downstream area of ​​the river after preliminary restoration, the ratio of water chestnut, hornwort and charophyte was 2:3:1, and the planting density was 30 plants / m2 , the planting area is 20% of the total area in the downstream area;

[0053] (4) After planting the aquatic plants, a biological growth promoter is applied at a dosage of 20 μL / L. The biological growth promoter is composed of the following raw materials in percentage by mass: 0.2% iron, 0.3% zinc, 0.3% magnesium, 1.5% L-cysteine, 4.5% vitamin B, 2.5% biotin, 0.5% plant growth hormone, 0.5% cytokinin, 1% gibberellin, 2% protease, 2% cellulase, 1% hydroxypropyl methylcellulose and the remainder water.

[0054] Example 3

[0055] A method for repairing heavy metal pollution in a river by composite planting of aquatic plants, comprising the following steps:

[0056] (1) A microbial agent was applied to the water body of the river to be repaired at an application rate of 2 mL / L for preliminary repair. The microbial agent was EM bacteria, Bacillus subtilis, Bacillus cereus and actinomycetes in a mass ratio of 1:10.5:8:12.5, and the effective viable bacterial count of each bacterium in the microbial agent was 12×10 7 cfu / mL;

[0057] (2) Floating beds were set up in the middle reaches of the river after the initial restoration, and duckweed and water hyacinth were planted in a ratio of 4:1, with a planting density of 26 plants / m 2 , the planting area is 20% of the total area in the midstream region;

[0058] (3) In the downstream area of ​​the river after preliminary restoration, the ratio of water chestnuts and charophytes was 3:1.5, and the planting density was 36 plants / m 2 , the planting area is 25% of the total area in the downstream area;

[0059] (4) After the aquatic plants are planted, a biological growth promoter is applied at a dosage of 32 μL / L. The biological growth promoter is composed of the following raw materials in percentage by weight: 0.7% zinc, 0.3% magnesium, 1.8% L-cysteine, 6.5% vitamin B, 1.5% plant growth hormone, 1.7% gibberellin, 5% cellulase, 1.5% hydroxypropyl methylcellulose and the remainder water.

[0060] Example 4

[0061] A method for repairing heavy metal pollution in a river by composite planting of aquatic plants, comprising the following steps:

[0062] (1) A microbial agent was applied to the water body of the river to be repaired at a dosage of 2 mL / L for preliminary repair. The microbial agent was EM bacteria, Bacillus subtilis and Bacillus cereus in a mass ratio of 1:10.5:8, and the effective viable count of each bacterium in the microbial agent was 12×10 7 cfu / mL;

[0063] (2) Floating beds were set up in the middle reaches of the river after the initial restoration, and water chestnuts, duckweed and water hyacinth were planted in a ratio of 1.5:4:1, with a planting density of 26 plants / m 2 , the planting area is 20% of the total area in the midstream region;

[0064] (3) In the downstream area of ​​the river after preliminary restoration, the ratio of water chestnuts, black algae and charophyte was directly planted at 3:2:1.5, and the planting density was 36 plants / m 2 , the planting area is 25% of the total area in the downstream area;

[0065] (4) After the aquatic plants are planted, a biological growth promoter is applied at a dosage of 32 μL / L. The biological growth promoter is composed of the following raw materials in percentage by weight: 0.6% iron, 0.4% zinc, 1.8% L-cysteine, 6.5% biotin, 3.2% plant growth hormone, 5% protease, 1.5% hydroxypropyl methylcellulose and the remainder water.

[0066] Example 5

[0067] A method for repairing heavy metal pollution in a river by composite planting of aquatic plants, comprising the following steps:

[0068] (1) A microbial agent was applied to the water body of the river to be repaired at a dosage of 2 mL / L for preliminary repair. The microbial agent was Bacillus subtilis, Bacillus cereus, Rhodopseudomonas sphaeroides and Actinomycetes in a mass ratio of 10.5:8:10:12.5, and the effective viable count of each bacterium in the microbial agent was 12×10 7 cfu / mL;

[0069] (2) Floating beds were set up in the middle reaches of the river after the initial restoration, and Nymphoides and Algae were planted in a ratio of 1.5:4, with a planting density of 26 plants / m 2 , the planting area is 20% of the total area in the midstream region;

[0070] (3) In the downstream area of ​​the river after preliminary restoration, the ratio of hornwort and charophyte was 2:1.5, and the planting density was 36 plants / m 2 , the planting area is 25% of the total area in the downstream area;

[0071] (4) After the aquatic plants are planted, a biological growth promoter is applied at a dosage of 32 μL / L. The biological growth promoter is composed of the following raw materials in percentage by weight: 1% zinc, 1.8% L-cysteine, 4% vitamin B, 2.5% biotin, 1.2% cytokinin, 2% gibberellin, 2% protease, 3% cellulase, 1.5% hydroxypropyl methylcellulose and the remainder water.

[0072] Comparative Example 1

[0073] The same as in Example 1, except that the microbial agent is not spread.

[0074] Comparative Example 2

[0075] As in Example 1, only the floating bed was set up in the middle reaches of the river after the preliminary restoration, and Nymphoides and Algae were planted in a ratio of 1.5:4, with a planting density of 26 plants / m 2 , the planting area is 20% of the total area of ​​the middle reaches" is replaced by "In the downstream area of ​​the river water body after preliminary restoration, a floating bed is set up and Nymphoides and Algae are planted in a ratio of 1.5:4, with a planting density of 26 plants / m 2 , the planted area accounts for 20% of the total area in the downstream area."

[0076] Comparative Example 3

[0077] As in Example 1, only the following was done: “In the downstream area of ​​the river after the preliminary restoration, the ratio of hornwort and charophyte was directly planted at 2:1.5, with a planting density of 36 plants / m 2 , the planting area is 25% of the total area of ​​the downstream area" is replaced by "In the middle reaches of the river water body after preliminary restoration, the ratio of 2:1.5 of duckweed and charophyte is directly planted, and the planting density is 36 plants / m 2 , the planting area accounts for 25% of the total area in the midstream region."

[0078] Comparative Example 4

[0079] The same as Example 1, except that the biological growth promoter is not spread.

[0080] Test Example 1

[0081] A simulated shallow water test platform was built, with a length and width of 9m×3m, a bottom mud depth of 0.4m, and a water depth of 0.7m. A heavy metal mixed solution was prepared with heavy metals Cd, Pb, Cr, and Hg as the pollution objects, and the heavy metal mixed solution was poured into the shallow water test platform, wherein the concentration of heavy metal Cd was 125μg / L, the concentration of heavy metal Pb was 35μg / L, the concentration of heavy metal Cr was 15μg / L, and the concentration of heavy metal Hg was 55μg / L. A total of 5 identical test platforms were set up, and repair treatments were carried out according to Example 1, Comparative Example 1, Comparative Example 2, Comparative Example 3, and Comparative Example 4, respectively. The heavy metal concentrations of different treatment groups were measured before and 3 months after repair, and the results are shown in Table 1.

[0082] Table 1 Heavy metal concentrations in different treatment groups before and 3 months after restoration

[0083]

[0084] It can be seen from Table 1 that the repair method of Example 1 can effectively reduce the concentration of various heavy metals in the water body and improve the heavy metal pollution situation.

[0085] In summary, by planting different aquatic plants in different areas and combining the use of microbial agents and biological growth promoters in the technical solution of the present invention, it is possible to promote the growth of aquatic plants and beneficial microorganisms, enhance the ability of plants to repair heavy metal pollution, and achieve the purpose of fully purifying water bodies.

[0086] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for repairing heavy metal pollution in rivers by composite planting of aquatic plants, characterized in that: The steps include: (1) Spreading microbial agents into the water body of the river to be repaired for preliminary repair; (2) Set up floating beds and plant floating-leaf plants in the midstream area of ​​the river after preliminary restoration, plant submerged plants in the downstream area, and then spread biological growth promoters.

2. The method according to claim 1, characterized in that: The microbial agent in step (1) includes any three or more of EM bacteria, Bacillus subtilis, Bacillus cereus, Rhodopseudomonas sphaeroides, and actinomycetes.

3. The method according to claim 2, characterized in that When the microbial agents are EM bacteria, Bacillus subtilis, Bacillus cereus, Rhodopseudomonas sphaeroides and actinomycetes, the mass ratio of EM bacteria, Bacillus subtilis, Bacillus cereus, Rhodopseudomonas sphaeroides and actinomycetes is (0.8-1.2):(9-13):(6-8):(8-12):(10-15).

4. The method according to claim 1, characterized in that: The effective viable count of each bacterium in the microbial agent is (5-15)×10 7 cfu / mL; the application amount of the microbial agent is 1-3mL / L.

5. The method according to claim 1, characterized in that The floating leaf plants in step (2) include any two or more of Nymphoides nymphoides, Dalbergia serrata, Water chestnut, and Eichhornia crassipes; The submerged plants include any two or more of water chestnuts, hornwort, chara, and black algae.

6. The method according to claim 1, characterized in that The planting density of the floating leaf plants in step (2) is 20-30 plants / m 2 , the planting area is 15-25% of the total area in the midstream region; The planting density of the submerged plants is 30-40 plants / m 2 , the planting area accounts for 20-30% of the total area in the downstream area.

7. The method according to claim 1, characterized in that The biological growth promoter in step (2) comprises the following raw materials in percentage by weight: 0.8-1.2% trace elements, 1.5-2% amino acids, 6-7% complex vitamins, 2-4% growth-promoting ingredients, 4-6% biological enzymes, 1-2% water-soluble polymers and the remainder water.

8. The method according to claim 7, characterized in that The trace elements include any one or more of iron, zinc or magnesium; The amino acids include L-cysteine; The vitamin complex includes vitamin B and / or biotin; The growth-promoting ingredients include any one or more of auxin, cytokinin or gibberellin; The biological enzymes include protease and / or cellulase; The water-soluble polymer includes hypromellose or hydroxypropyl cellulose.

9. The method according to claim 1, characterized in that: The application amount of the biological growth promoter is 20-40 μL / L.

Citation Information

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