Alcohol effect dispelling, liver protecting and stomach protecting agent and preparation method thereof

Abstract

The invention relates to the technical field of traditional Chinese medicines, in particular to a hangover-alleviating, liver-protecting and stomach-protecting agent and a preparation method thereof. The alcohol effect dispelling, liver protecting and stomach protecting agent is prepared from the following raw material components in parts by mass: 1-10 parts of peristrophe japonica, 1-10 parts of penthorum chinense pursh, 1-10 parts of flos puerariae, 1-10 parts of radix puerariae, 1-10 parts of lalang grass rhizome, 1-10 parts of hovenia dulcis thunb and 1-5 parts of taurine. The traditional Chinese medicine composition has better hangover-alleviating and liver-protecting effects, obviously shortens the hangover-alleviating and sobering time, is taken before drinking, takes effect faster, has the effects of alleviating hangover, inducing resuscitation, strengthening the spleen and stomach, calming the adverse-rising energy, arresting vomiting and protecting the liver, reduces AST and ALT in human serum, can enhance the activity of ethanol dehydrogenase and acetaldehyde dehydrogenase in the human liver, and promotes ethanol metabolism.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicines, and in particular to an alcohol-relieving, liver-protecting and stomach-protecting agent and a preparation method thereof. Background Art

[0002] People who drink alcohol frequently need to take measures to sober up. There are many methods to sober up, and there are also many drugs to sober up. However, these drugs have the following defects: the taste is difficult to accept, some of them can sober up, but they cannot protect against chemical liver damage, they have a single function and cannot be used for people with different symptoms of sobering up; with the development of technology, some drugs can protect the liver, but the effect is not good, or they have certain toxic side effects. Summary of the invention

[0003] The purpose of the present invention is to provide a hangover-relieving, liver-protecting and stomach-protecting agent and a preparation method thereof in view of the above-mentioned deficiencies in the prior art.

[0004] The alcohol-relieving, liver-protecting and stomach-protecting agent of the present invention is prepared from the following raw material components in parts by mass:

[0005]

[0006] Furthermore, VB6 0.001-0.01 parts is also included.

[0007] Furthermore, it also includes 0.001-0.01 parts of pyroglutamic acid.

[0008] Furthermore,

[0009]

[0010] Furthermore, the alcohol-relieving, liver-protecting and stomach-protecting agent is in the form of pills, powders, tablets, granules, capsules or oral liquids.

[0011] Furthermore, the tablet is a sugar-coated tablet, a film-coated tablet or an effervescent tablet.

[0012] A method for preparing the above-mentioned alcohol-detoxifying, liver-protecting and stomach-protecting agent comprises: crushing Herba Cynoglossi, Herba Cynoglossi, Puerariae lobatae, Puerariae lobatae, Imperatae cylindricae and Hovenia dulcis respectively and mixing them evenly, soaking them in water, decocting them and then extracting them by ultrasonic wave to obtain an extract, concentrating them under reduced pressure to obtain an extract, adding pharmaceutically acceptable excipients and / or drying to prepare the alcohol-detoxifying, liver-protecting agent.

[0013] A method for preparing the above-mentioned alcohol-detoxifying, liver-protecting and stomach-protecting agent, wherein the alcohol-detoxifying, liver-protecting and stomach-protecting agent is an oral liquid, wherein each raw material is weighed, and the nine-headed lion grass, yellow grass, kudzu flower, kudzu root, white grass root and Hovenia dulcis are respectively crushed and mixed evenly, soaked in water, decocted and extracted by ultrasonic wave to obtain an extract, taurine, VB6 and pyroglutamic acid are added to the extract and stirred thoroughly to obtain a mixed solution; the mixed solution is sterilized at normal pressure and cooled to room temperature to obtain the obtained solution.

[0014] Furthermore, in each decoction and extraction process, the mass ratio of the total mass of each raw material to the mass of water is 1:5-10.

[0015] Furthermore, the decoction includes mixing the raw materials with water and then soaking them for 30-60 minutes, boiling them over high heat and then decocting them over low heat for 60-120 minutes.

[0016] The alcohol-detoxifying, liver-protecting and stomach-protecting agent provided by the present invention comprises multiple components such as scutellaria baicalensis, kudzu flower, kudzu root, Imperata cylindrica root, Hovenia dulcis fruit, taurine, etc. Among them, scutellaria baicalensis has the effect of clearing away heat and detoxifying, and helps to relieve stomach discomfort caused by alcohol, such as symptoms such as stomach heat and gastritis. The n-butanol part of scutellaria baicalensis can significantly inhibit the increase of ALT and AST in rat serum, showing a significant liver-protecting effect.

[0017] The herb contains a variety of chemical components, mainly flavonoids, lignans, coumarins, acetophenones, tannins, triterpenes, organic acids, esters and volatile oils. These components work together to make the herb have the effect of clearing away heat and detoxifying, improving liver function, accelerating the metabolism of moisture in the body, promoting blood circulation and removing blood stasis, preventing thrombosis, protecting the liver, enhancing the liver's detoxification function, reducing the damage of drugs and viruses to the liver, and accelerating the decomposition and metabolism of alcohol in the human body;

[0018] Pueraria flower is a good promoter of liver metabolism. It affects the absorption and metabolism of alcohol. It can play a role in sobering up through anti-oxidation, liver protection, and protection of the central nervous system. It has a protective effect on liver cell damage caused by alcohol. The daidzein, flavonoids, and puerarin in Pueraria flower can improve alcohol metabolism disorders and liver function, and also have a certain effect on organ disorders and digestive tract dysfunction caused by alcohol.

[0019] Pueraria root extract has the effects of inhibiting alcohol absorption, accelerating alcohol metabolism, increasing alcohol tolerance, reducing mortality and reducing liver damage. It can alleviate alcoholic liver damage caused by excessive ethanol intake. It can protect the liver and prevent alcoholic liver fibrosis by effectively inhibiting the proliferation and activation of hepatic stellate cells. It can also slow down alcohol absorption. The flavonoids in Pueraria root can decompose ethanol and reduce the damage of ethanol to the brain. It also has the effect of clearing rash and relieving fever. It can well relieve the burning sensation in the stomach caused by drinking and reduce the irritation of alcohol to the stomach and intestines.

[0020] Imperata root has the effects of reducing oxygen free radicals and anti-oxidation, can improve the body's antioxidant capacity, and has a certain protective effect on liver damage caused by alcohol poisoning. Imperata root can clear stomach heat and relieve stomach heat and vomiting, and vomiting will stop when the heat is cleared.

[0021] Taurine can reduce serum AST and ALT as well as liver TNF-α levels and MMP-9 activity in alcohol-fed rats, and reduce alcohol-induced liver cell damage. Taurine can protect the gastric mucosa and reduce the direct stimulation and damage of alcohol to the gastric mucosa.

[0022] The invention has better alcohol sobering and liver protection effects, and the alcohol sobering and sobering up time is significantly shortened. When taken before drinking, the effect is more rapid, and the invention has the effects of sobering up and refreshing the mind, strengthening the spleen and stomach, relieving adverse reactions and stopping vomiting, and protecting the liver. It can reduce AST and ALT in human serum, enhance the activities of alcohol dehydrogenase and acetaldehyde dehydrogenase in the human liver, and promote ethanol metabolism. DETAILED DESCRIPTION

[0023] The following are specific embodiments of the present invention, which further describe the technical solutions of the present invention, but the present invention is not limited to these embodiments.

[0024] Example

[0025] The preparation method of a hangover-relieving, liver-protecting and stomach-protecting agent is that the hangover-relieving, liver-protecting and stomach-protecting agent is an oral liquid, wherein each raw material is weighed, and nine-headed lion grass, yellow grass, kudzu flower, kudzu root, white imperata root and Hovenia dulcis fruit are crushed and mixed evenly respectively, soaked in water (the mass ratio of the total mass of each raw material to the mass of water is 1:5), boiled and extracted by ultrasonic wave to obtain an extract, taurine, VB6 and pyroglutamic acid are added to the extract and stirred thoroughly to obtain a mixed solution; the mixed solution is sterilized at normal pressure and cooled to room temperature to obtain the obtained solution.

[0026] Table 1 Formula table of various embodiments

[0027]

[0028]

[0029] Test Example 1

[0030] According to the relevant requirements of the Technical Specifications for Inspection and Evaluation of Health Food (2003 edition) of the Ministry of Health, the effects of the present invention on serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) of mice were investigated.

[0031] Fifty healthy mice of equivalent weight were taken, half of which were male and half were female, and randomly divided into 5 groups. The experimental group used the products of Example 1 and Comparative Example 1, and the others were set up as a normal group, a model group (CCl4 modeling) and a positive control group (the positive drug was Neptune Jinzun). The experiment was started after one week of adaptation.

[0032] In the experiment, each group was gavaged with the set amount of alcohol or medicine every day. After the end of the dosing on the 44th day, all animals were fasted for one day. One hour after the dosing on the 45th day, blood was drawn from the orbital vein of the mice and serum was collected for testing. At the same time, the liver was quickly dissected and preserved for testing as required.

[0033] ALT and AST are mainly present in the cytoplasm of liver cells. When liver cells are damaged, the intracellular transaminase can enter the blood, causing the ALT and AST levels in the blood to increase. There is a significant difference compared with mice given alcohol (P<0.01). The results are shown in Table 2:

[0034] Table 2 Effect of preventive administration of the present invention on serum ALT & AST in mice

[0035] Dosage (mg / kg) ALT(U / L) AST(U / L) Normal group 0 5.02±5.73 32.36±11.93 Model Group 0 47.01±34.56 73.56±12.34 Positive group 358 38.17±20.90 68.35±15.01 Example 1 180 10.23±7.95 45.09±14.07 Comparative Example 1 180 10.23±7.95 45.09±14.07

[0036] The data in the ALT and AST columns in Table 1 are mean values ​​± standard deviations. Compared with the normal group, the ALT and AST indicators of the model group were higher than those of the normal group, and the difference was statistically significant (p<0.01), indicating that the model was successfully established. It can be seen from the above table that the alcohol-detoxifying, liver-protecting and stomach-protecting agent of the present invention can reduce serum ALT and AST in model mice, and the preventive effect is better than that of the positive control group, indicating that the alcohol-detoxifying, liver-protecting and oral composition of the present invention can be absorbed through the digestive tract, protect liver cells, inhibit the increase of ALT and AST in liver tissue, stabilize liver cell membranes, and inhibit the release of ALT and AST into the blood, thereby effectively preventing and treating alcoholic liver damage.

[0037] Test Example 2

[0038] Determination of ethanol content in rat serum

[0039] 100 SD rats were randomly divided into a blank group, a model group, a positive drug group (Neptune Jinzun, Shenzhen Neptune Health Technology Co., Ltd.), Examples 1-4, and Comparative Examples 1-3. The blank group was given 0.02 mL / g of distilled water, and the other groups were given 0.02 mL / g of Erguotou; 30 minutes later, the rats in each group of the Examples and Comparative Examples were gavaged with the alcohol-relieving, liver-protecting, and stomach-protecting agents prepared in each Example and Comparative Example, the blank group and the model were given an equal volume of distilled water, and the rats in the positive group were gavaged with Neptune Jinzun tablets diluted with distilled water, so that the dosage of Neptune Jinzun was 0.01 g / g; 60 minutes later, the ethanol content in the serum was determined, and the test results are shown in Table 3.

[0040] Table 3 Effect of alcohol-relieving and liver-protecting agents on ethanol content in rat serum

[0041]

[0042]

[0043] As can be seen from Table 3, the ethanol content in the serum of rats in Example 1 to Example 4 groups using the Chinese medicine formula and preparation method of the present invention is significantly lower than that in the model group and Comparative Examples 1-3. Compared with the positive drug group, the ethanol content in the serum of rats in Example 1 to Example 4 groups is also significantly lower, indicating that the Chinese medicine effect obtained by the Chinese medicine formula provided by the present invention is better than the commonly used drugs on the market.

[0044] Test Example 3

[0045] The beverage with the effect of sobering up and protecting liver in Example 1-4 was used as the test solution, 1.5 mL of sodium pyrophosphate solution (pH value was 8.8), 1.0 mL of oxidized coenzyme (INAD+) (concentration was 0.027 mol / L), 0.5 mL of ethanol aqueous solution (concentration was 11.5%) and 0.1 mL of the test solution were added to the test tube, and the mixture was placed in a 25° water bath for 5 min after being fully shaken and evenly mixed. After the end of the insulation, 0.1 mL of 0.25 U / mL of alcohol dehydrogenase (ADH) was immediately added to the test tube, and the absorbance A340 was measured at a wavelength of 340 nm by a spectrophotometer immediately after mixing. A340 was recorded every 10 seconds for 5 min. 0.5 mL of distilled water was used to replace the 11.5% ethanol aqueous solution as a blank control for zero adjustment, 0.1 mL of distilled water was used to replace the sample solution as a negative control, and an equal volume of commercially available alcohol sobering drug HANGOVER (Medicura) was used as a positive control. The activity unit of ADH is calculated. The activity of ADH is expressed in nanomoles of NADH generated per minute (nmol / min). The data of the hangover effect are shown in Table 4:

[0046] Table 4

[0047] Group ADH activation rate (%) Blank Group 0 Negative control group 0 Positive control group 14.78 Example 1 26.15 Example 2 24.13 Example 3 22.77 Example 4 23.45 Comparative Example 1 13.21 Comparative Example 2 15.35 Comparative Example 3 14.78

[0048] The above test data show that the beverages with sobering up and protecting liver functions provided by Examples 1-4 of the present invention can enhance the ADH activation rate. This indicates that the beverages with sobering up and protecting liver functions provided by the present invention have a significant sobering up effect, can avoid the occurrence of acute alcohol poisoning to a certain extent, can effectively increase the duration of drunkenness, shorten the duration of sleep after drunkenness, and have a gain effect on the recovery of the central nervous system to a certain extent.

[0049] For matters not mentioned above, the prior art applies.

[0050] Although some specific embodiments of the present invention have been described in detail through examples, those skilled in the art should understand that the above examples are for illustration only and are not intended to limit the scope of the present invention. Those skilled in the art to which the present invention belongs may make various modifications or supplements to the specific embodiments described or replace them in a similar manner, but they will not deviate from the direction of the present invention or exceed the scope defined by the attached claims. Those skilled in the art should understand that any modifications, equivalent substitutions, improvements, etc. made to the above embodiments based on the technical essence of the present invention should be included in the protection scope of the present invention.

Claims

1. A hangover-relieving, liver-protecting and stomach-protecting agent, characterized in that: Prepared from the following raw material components by mass:

2. The alcohol-relieving, liver-protecting and stomach-protecting agent according to claim 1, characterized in that: Also includes VB60.001-0.01 copies.

3. The alcohol-relieving, liver-protecting and stomach-protecting agent according to claim 1, characterized in that: It also includes 0.001-0.01 parts of pyroglutamic acid.

4. The alcohol-relieving, liver-protecting and stomach-protecting agent according to claim 1, characterized in that:

5. The alcohol-relieving, liver-protecting and stomach-protecting agent according to claim 1, characterized in that: The alcohol-relieving, liver-protecting and stomach-protecting agent is in the form of pills, powders, tablets, granules, capsules or oral liquids.

6. The alcohol-relieving, liver-protecting and stomach-protecting agent according to claim 1, characterized in that: The tablet is a sugar-coated tablet, a film-coated tablet or an effervescent tablet.

7. A method for preparing the alcohol-relieving, liver-protecting and stomach-protecting agent according to any one of claims 1 to 6, characterized in that: Grind and mix the herb of nine-headed lion's head, herb of yellow grass, kudzu flower, kudzu root, cyperus root and Hovenia dulcis respectively, soak them in water, decoct and extract them by ultrasonic wave to obtain an extract, concentrate under reduced pressure to obtain an extract, add pharmaceutically acceptable excipients and / or dry them to prepare the alcohol-detoxifying and liver-protecting agent.

8. A method for preparing the alcohol-relieving, liver-protecting and stomach-protecting agent according to any one of claims 1 to 6, characterized in that: The hangover-relieving, liver-protecting and stomach-protecting agent is an oral liquid, and each raw material is weighed according to the raw material ratio of the above-mentioned beverage with the effect of sobering up and protecting the liver, and nine-headed lion grass, yellow grass, kudzu flower, kudzu root, white grass root, and Hovenia dulcis are respectively crushed and mixed evenly, soaked in water, decocted and then subjected to ultrasonic extraction to obtain an extract, taurine, VB6 and pyroglutamic acid are added to the extract and stirred thoroughly to obtain a mixed solution; the mixed solution is sterilized at normal pressure and cooled to room temperature to obtain the product.

9. The preparation method according to claim 8, characterized in that: During each decoction and extraction process, the mass ratio of the total mass of each raw material to the mass of water is 1:5-10.

10. The method according to claim 8, characterized in that The decoction includes mixing the raw materials with water and then soaking them for 30-60 minutes, boiling them over high heat and then decocting them over low heat for 60-120 minutes.

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