Preparation method of blood perfusion diagnosis and treatment device based on guanosine supramolecular gel

Abstract

The invention belongs to the field of medical treatment, and particularly relates to a preparation method of a blood perfusion diagnosis and treatment device based on guanosine supramolecular gel, which comprises the following steps: preparing guanosine-boric acid supramolecular hydrogel combined with TMPyP, the guanosine-boric acid supramolecular hydrogel being GB hydrogel; the GB hydrogel combined with the TMPyP probe is attached to the surface of the U-shaped optical fiber; the optical fiber attached with the GB hydrogel is immersed in a lead ion solution for adsorption, meanwhile, the adsorption amount is monitored through electrical and optical bimodal signals, after adsorption is completed, adsorbed lead ions can be replaced for regeneration, and cyclic adsorption is achieved. The adsorption material for blood perfusion, which has high selectivity, excellent blood compatibility, reproducibility and fluid shearing resistance, is integrated with an optical fiber detection device, so that the integration of treatment and monitoring is realized; the GB hydrogel realizes selective adsorption and real-time monitoring of heavy metal ions through a G4 tetramer structure unit.

Description

Technical Field

[0001] The invention belongs to the medical field, and in particular relates to a method for preparing a blood perfusion diagnosis and treatment device based on guanosine supramolecular gel. Background Art

[0002] Hemoperfusion technology is a clinical treatment method that uses adsorption materials to extract toxic substances from the blood. This technology has many indications and is mainly used in acute and chronic drug poisoning, adsorption of molecular toxins in uremia, liver disease and adsorption of pathogenic factors in the immune field. Hemoperfusion devices are divided into activated carbon hemoperfusion and resin hemoperfusion. Since hemoperfusion devices can be used directly on dialysis machines without the need for dedicated perfusion equipment, they are widely used in clinical applications in hospitals.

[0003] At present, the research and development of new blood perfusion materials themselves have ignored the selective removal of heavy metals, resulting in the loss of normal blood components. Other coping strategies require the intervention of more equipment, such as hemodialysis filtration machines, which increases the cost of use and the difficulty of clinical transformation. It is impossible to achieve recycling and cannot perform visual / fiber optic quantitative dual-modality real-time monitoring during the blood perfusion process. Summary of the invention

[0004] In order to overcome the shortcomings of the prior art, the present invention provides a method for preparing a hemoperfusion diagnostic and therapeutic device based on guanosine supramolecular gel, so as to solve the problem that the current development and research on new hemoperfusion materials themselves ignores the selective removal of lead ions, resulting in the loss of normal blood components, while other coping strategies require the intervention of more equipment, such as hemodialysis filtration machines, thereby increasing the cost of use and the difficulty of clinical transformation.

[0005] One embodiment of the present invention provides a method for preparing a blood perfusion diagnostic and therapeutic device based on guanosine supramolecular gel, the method comprising: preparing a guanosine-boric acid supramolecular hydrogel combined with TMPyP, wherein the guanosine-boric acid supramolecular hydrogel is a GB hydrogel; Then, the GB hydrogel combined with the TMPyP probe is attached to the surface of the U-shaped optical fiber; The optical fiber attached with GB hydrogel is immersed in a lead ion solution for adsorption. In the solution, the lead ions compete with the sodium ions in the GB gel to form adsorption. EDTA sodium salt chelates the lead ions in the GB gel and regenerates and replaces them with sodium ions, thereby realizing the cyclic adsorption of GB hydrogel, that is, forming an intelligent blood perfusion device based on guanosine supramolecular gel.

[0006] The present invention provides a method for preparing a blood perfusion diagnostic and therapeutic device based on guanosine supramolecular gel, which provides an adsorption material for blood perfusion with high selectivity, excellent blood compatibility, renewability, and resistance to fluid shear. Compared with the non-selective adsorption of traditional porous materials (such as activated carbon and resin), GB hydrogel achieves selective adsorption of heavy metal ions through the G4 tetramer result unit, avoiding the side effects caused by protein and cell adsorption. In addition, its self-healing property can resist blood flow shear, thereby achieving recycling, achieving economy, green sustainability, and reducing the cost of use.

[0007] In one embodiment, the TMPyP is α, β, γ, δ-tetrakis(1-methylpyridin-4-yl)porphine p-toluenesulfonate.

[0008] In one embodiment, the U-shaped optical fiber is an optical fiber sensor based on surface plasmon resonance.

[0009] In one embodiment, the prepared perfusion device is used for the selective removal of heavy metal ions and can simultaneously realize real-time monitoring of toxic components in blood.

[0010] In one embodiment, the prepared perfusion device is used for selective removal of heavy metal ions and simultaneous real-time monitoring of toxic components in blood.

[0011] In one embodiment, the preparation of TMPyP-bound guanosine-boronic acid supramolecular hydrogel comprises: To prepare 5 wt % GB hydrogel, 0.566 g guanosine and 0.056 g boric acid were mixed in 10 ml distilled water and shaken on a vortex mixer for 5 min; The mixture was heated in an oil bath at 95°C with magnetic stirring, and 2.5 ml of NaOH solution was added simultaneously; When the solution turned from turbid to clear, the heating was stopped and the solution was cooled to room temperature to obtain GB hydrogel.

[0012] In one embodiment, the optical fiber attached with GB hydrogel is immersed in a lead ion solution for adsorption, the lead ions in the solution compete with the sodium ions in the GB gel to form adsorption, EDTA sodium salt chelates the lead ions in the GB gel, and regenerates and replaces them with sodium ions, thereby realizing the cyclic adsorption of GB hydrogel, that is, forming an intelligent blood perfusion device based on guanosine supramolecular gel, including: Prepare a visualized regenerative and recyclable optical fiber device, and mix 150 μM TMPyP in the GB hydrogel preparation process to prepare a TMPyP-bound hydrogel; The GB hydrogel with the TMPyP probe was deposited on the U-shaped optical fiber. The temperature of the GB gel was raised to above 95°C to turn it into a solution state. Then, the optical fiber was immersed in the GB gel solution. After a layer of gel adhered to the surface of the optical fiber, it was picked up and dried naturally. A U-shaped optical fiber with GB hydrogel attached was obtained by a dip-coating method. The optical fiber with GB hydrogel attached was immersed in a lead ion solution for adsorption-regeneration cycle. In the solution, lead ions competed with sodium ions in the GB gel to form adsorption. EDTA sodium salt chelated the lead ions in the GB gel and regenerated and replaced them with sodium ions to achieve cyclic adsorption of GB hydrogel. The SPR spectrum and electrochemical signals of the adsorption-regeneration cycle were recorded by an optical fiber sensor, and finally an intelligent blood perfusion device based on GB hydrogel was prepared.

[0013] The method for preparing a blood perfusion diagnostic and therapeutic device based on guanosine supramolecular gel provided by the above technical solution has the following beneficial effects: 1. The present invention provides an adsorption material for blood perfusion with high selectivity, excellent blood compatibility, renewability and resistance to fluid shear. Compared with the non-selective adsorption of traditional porous materials (such as activated carbon and resin), GB hydrogel achieves selective adsorption of heavy metal ions through G4 tetramer units to avoid side effects. In addition, its self-healing property can resist blood flow shear, thereby achieving recycling, achieving economy, green sustainability, and reducing the cost of use.

[0014] 2. The present invention introduces the real-time multimodal monitoring function of visual qualitative / fiber optic biosensing technology quantitative into the blood perfusion component. Currently, it is mostly impossible to monitor the blood lead level in real time during clinical blood purification processes. By introducing TMPyP probes into GB hydrogels, it is given the characteristic of color change after adsorbing lead ions. At the same time, fiber optic biosensing technology based on surface plasmon resonance (SPR) is used to quantitatively monitor the changes in optical and electrical signals of the gel after adsorbing lead ions, thereby realizing visual / fiber optic quantitative dual-modal real-time monitoring during blood perfusion.

[0015] 3. The present invention realizes the integration of selective adsorption and real-time dual-modal monitoring on a single GB hydrogel. Based on the unique chemical structure and optical properties of GB gel assembled by small molecule bases and metal ions, selective ion adsorption and refractive properties that change with adsorption can be realized in a single hydrogel with simple components. The application of the blood perfusion device based on guanosine supramolecular gel visualized qualitative / optical fiber quantitative dual-modal real-time monitoring adopted by the present invention has broad promotion and application value. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying creative work.

[0017] Figure 1 The scanning electron microscopic comparison images of the freeze-dried GB hydrogel (a) before and (b) after treatment with the lead ion solution of the present invention; Figure 2 For the blood lead adsorption of the present invention: (a) blood lead removal efficiency during the treatment cycle using GB hydrogel; (b) residual blood lead concentration measured by the adsorption cycle; Figure 3 This is a color change diagram of the GB hydrogel combined with TMPyP after being treated with different concentrations of lead ion (PB2+) solutions of the present invention; Figure 4 This is a device diagram of the intelligent blood perfusion device of the present invention; Figure 5 The SPR wavelength shift diagram of the optical fiber attached with GB hydrogel of the present invention when a -1V potential is applied in different solutions; Figure 6 is a selective adsorption diagram of the optical / electrochemical reaction GB hydrogel of the present invention; Figure 7 This is the adsorption (deposition)-desorption (reduction) regeneration cycle diagram of 2 μM lead ions of the present invention. DETAILED DESCRIPTION

[0018] Embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0019] In the description of the present invention, it should be understood that descriptions involving orientations, such as up, down, front, back, left, right, etc., and orientations or positional relationships indicated are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0020] In the description of the present invention, "several" means one or more, "more" means more than two, "greater than", "less than", "exceed" etc. are understood as not including the number itself, and "above", "below", "within" etc. are understood as including the number itself. If there is a description of "first" or "second", it is only used for the purpose of distinguishing the technical features, and cannot be understood as indicating or implying the relative importance or implicitly indicating the number of the indicated technical features or implicitly indicating the order of the indicated technical features.

[0021] In the description of the present invention, unless otherwise clearly defined, terms such as setting, installing, connecting, etc. should be understood in a broad sense, and technicians in the relevant technical field can reasonably determine the specific meanings of the above terms in the present invention based on the specific content of the technical solution.

[0022] Combination Figures 1 to 7 As shown, one embodiment of the present invention provides a method for preparing a blood perfusion diagnostic and therapeutic device based on guanosine supramolecular gel, the method comprising: preparing a guanosine-boric acid supramolecular hydrogel combined with TMPyP, wherein the guanosine-boric acid supramolecular hydrogel is a GB hydrogel; Then, the GB hydrogel combined with the TMPyP probe is attached to the surface of the U-shaped optical fiber; The optical fiber attached with GB hydrogel is immersed in a lead ion solution for adsorption. In the solution, the lead ions compete with the sodium ions in the GB gel to form adsorption. EDTA sodium salt chelates the lead ions in the GB gel and regenerates and replaces them with sodium ions, thereby realizing the cyclic adsorption of GB hydrogel, that is, forming an intelligent blood perfusion device based on guanosine supramolecular gel.

[0023] The present invention provides a method for preparing a blood perfusion diagnostic and therapeutic device based on guanosine supramolecular gel, which provides an adsorption material for blood perfusion with high selectivity, excellent blood compatibility, renewability, and resistance to fluid shear. Compared with the non-selective adsorption of traditional porous materials (such as activated carbon and resin), GB hydrogel achieves selective adsorption of heavy metal ions through the G4 tetramer result unit to avoid side effects. In addition, its self-healing property can resist blood flow shear, thereby achieving recycling, achieving economy, green sustainability, and reducing the cost of use.

[0024] It should be noted that the primary purpose of the present invention is to provide a method for preparing a blood perfusion diagnostic and therapeutic device based on guanosine supramolecular gel, in view of the low selectivity of conventional adsorbents in existing blood perfusion devices, which easily cause loss of blood components and form side effects, and to develop a new type of blood perfusion device with selective removal of lead ions and capable of real-time monitoring of blood toxic components.

[0025] Furthermore, guanosine-boric acid supramolecular hydrogel (GB hydrogel) was prepared to obtain a blood perfusion adsorbent material with blood lead-specific adsorption, good stability and self-healing properties.

[0026] In one embodiment, the TMPyP is α, β, γ, δ-tetrakis(1-methylpyridin-4-yl)porphine p-toluenesulfonate.

[0027] In this example, a GB hydrogel combined with α, β, γ, δ-tetrakis(1-methylpyridin-4-yl)porphine p-toluenesulfonate (TMPyP) was prepared to obtain a visual and qualitative blood perfusion adsorbent material.

[0028] In one embodiment, the U-shaped optical fiber is an optical fiber sensor based on surface plasmon resonance.

[0029] In this embodiment, a regenerative and recyclable optical fiber device is prepared, and a GB hydrogel combined with a TMPyP probe is attached to the surface of a U-shaped optical fiber. The optical fiber attached with the GB hydrogel is immersed in a lead ion solution for an adsorption-regeneration cycle. Adsorption is formed by competing with the sodium ions in the GB gel in the solution, and EDTA sodium salt chelates the lead ions in the GB gel and regenerates and replaces them with sodium ions to achieve cyclic adsorption of the GB hydrogel, thereby obtaining a regenerative and recyclable optical fiber device.

[0030] It should be noted that the intelligent blood perfusion device of the present invention selectively adsorbs and removes lead ions during the blood purification process and monitors the toxicity of blood components in real time. Its characteristics are: the prepared GB hydrogel is combined with TMPyP, and a concentration-dependent color change occurs when it comes into contact with lead ions, thereby realizing visual qualitative monitoring, thereby obtaining a qualitative and visual blood perfusion adsorbent material, and then by attaching the GB hydrogel combined with TMPyP to the surface of the optical fiber, ion competition and regeneration replacement are achieved, thereby realizing the cyclic adsorption of lead ions by the guanosine supramolecular gel intelligent blood perfusion device.

[0031] In one embodiment, the prepared perfusion device is used for the selective removal of heavy metal ions and can simultaneously realize real-time monitoring of toxic components in blood.

[0032] In this embodiment, the real-time multimodal monitoring function of qualitative visualization / quantitative fiber optic biosensing technology is introduced into the blood perfusion component. Currently, it is mostly impossible to monitor the blood lead level in real time during clinical blood purification processes. The present invention introduces TMPyP probes into GB hydrogels to give them the characteristic of color change after adsorbing lead ions, and simultaneously uses fiber optic biosensing technology based on surface plasmon resonance (SPR) to quantitatively monitor the changes in optical and electrical signals of the gel after adsorbing lead ions, thereby realizing visual / fiber optic quantitative dual-modal real-time monitoring during blood perfusion.

[0033] In one embodiment, the prepared perfusion device is used for selective removal of heavy metal ions and simultaneous real-time monitoring of toxic components in blood.

[0034] In one embodiment, the preparation of TMPyP-bound guanosine-boronic acid supramolecular hydrogel comprises: To prepare 5 wt % GB hydrogel, 0.566 g guanosine and 0.056 g boric acid were mixed in 10 ml distilled water and shaken on a vortex mixer for 5 min; The mixture was heated in an oil bath at 95°C with magnetic stirring, and 2.5 ml of NaOH solution was added simultaneously; When the solution turned from turbid to clear, the heating was stopped and the solution was cooled to room temperature to obtain GB hydrogel.

[0035] In this example, to prepare a 5 wt% GB hydrogel, 0.566 g of guanosine and 0.056 g of boric acid were mixed in 10 ml of distilled water and oscillated on a vortex mixer for 5 min. The mixture was heated in a 95°C oil bath and magnetically stirred. At the same time, 2.5 ml of NaOH solution (16 mg / mL) was added. When the solution became clear from turbid, the heating was stopped and the solution was cooled to room temperature to obtain a GB hydrogel. The hydrogel was incubated in physiological saline for 4 h to achieve sodium ion adsorption saturation, thereby stabilizing the hydrogel. The mass-to-volume ratio (w / v) of the GB hydrogel involved was the ratio of the dry weight of the hydrogel to the volume of the solution. Furthermore, in order to prove that the GB hydrogel has the expected lead ion adsorption performance, the GB hydrogel with / without lead ions was characterized by scanning electron microscopy (SEM). By observing the microscopic morphology of the GB hydrogel before and after lead ion treatment by freeze-drying, the pore area of ​​the gel was significantly reduced after adsorption of lead ions, making the gel more dense (e.g. Figure 1 The results show that the cross-linking density of GB hydrogel increases after adsorbing lead ions, which proves the effective adsorption of lead ions by GB hydrogel.

[0036] Furthermore, in order to prove the adsorption effect of GB hydrogel on blood lead, a multiple cycle adsorption mode was used to evaluate the potential of GB hydrogel as a blood lead adsorbent. In the single time-dependent mode, GB hydrogel adsorbed more than 30% of blood lead within 0.5 h. In the multiple cycle adsorption mode, 3 adsorption cycles achieved an adsorption rate of 60%. The cyclic adsorption of multiple adsorption modules on the surface will further improve the removal efficiency of blood lead (e.g. Figure 2 as shown).

[0037] In one embodiment, the optical fiber attached with GB hydrogel is immersed in a lead ion solution for adsorption, the lead ions in the solution compete with the sodium ions in the GB gel to form adsorption, EDTA sodium salt chelates the lead ions in the GB gel, and regenerates and replaces them with sodium ions, thereby realizing the cyclic adsorption of GB hydrogel, that is, forming an intelligent blood perfusion device based on guanosine supramolecular gel, including: Prepare a visualized regenerative and recyclable optical fiber device, and mix 150 μM TMPyP in the GB hydrogel preparation process to prepare a TMPyP-bound hydrogel; The GB hydrogel with the TMPyP probe was deposited on the U-shaped optical fiber. The temperature of the GB gel was raised to above 95°C to turn it into a solution state. Then, the optical fiber was immersed in the GB gel solution. After a layer of gel adhered to the surface of the optical fiber, it was picked up and dried naturally. A U-shaped optical fiber with GB hydrogel attached was obtained by a dip-coating method. The optical fiber with GB hydrogel attached was immersed in a lead ion solution for adsorption-regeneration cycle. In the solution, lead ions competed with sodium ions in the GB gel to form adsorption. EDTA sodium salt chelated the lead ions in the GB gel and regenerated and replaced them with sodium ions to achieve cyclic adsorption of GB hydrogel. The SPR spectrum and electrochemical signals of the adsorption-regeneration cycle were recorded by an optical fiber sensor, and finally an intelligent blood perfusion device based on GB hydrogel was prepared.

[0038] In this embodiment, TMPyP (150 μM) was mixed in the GB hydrogel preparation process to prepare a TMPyP-bound hydrogel, and after contact with lead ions, the TMPyP bound to the GB hydrogel would undergo a color change visible to the naked eye (e.g. Figure 3 as shown).

[0039] Furthermore, in order to deposit the GB hydrogel with the TMPyP probe introduced on the U-shaped optical fiber, the temperature of the GB gel was raised to above 95°C to turn it into a solution state, and then the optical fiber was immersed in the GB gel solution. After a layer of gel was attached to the surface of the optical fiber, it was taken up and dried naturally, and the U-shaped optical fiber with the GB hydrogel attached was obtained by a dip-coating method. The optical fiber with the GB hydrogel attached was immersed in a lead ion solution for adsorption-regeneration cycle. In the solution, the lead ions competed with the sodium ions in the GB gel to form adsorption, and the EDTA sodium salt chelated the lead ions in the GB gel and regenerated and replaced them with sodium ions to achieve the cyclic adsorption of the GB hydrogel. The SPR spectrum and electrochemical signals of the adsorption-regeneration cycle were recorded using an optical fiber sensor, and finally the preparation of a smart blood perfusion device based on GB hydrogel (such as Figure 4 As shown). By applying a potential to the fiber electrode and exciting the generation of an electrochemical double layer on the fiber surface, a pump signal light is transmitted to the fiber electrode to stimulate the surface plasmon laser resonance at the interface between the surface gold film and the electrolyte, and the in-situ optical signal feedback of the electrochemical adsorption behavior is achieved by matching the surface plasmon laser resonance with the local space of the electrochemical double layer. The environmental electrochemical behavior is monitored by an electrochemical workstation, and the optical signal detection equipment records the peak and valley optical wavelength changes of the surface plasmon laser resonance excitation spectrum, so as to achieve in-situ online monitoring of the surface electrosorption behavior of the fiber electrode, and can achieve real-time, in-situ and multi-modal in-situ characterization of the electrochemical adsorption behavior, which is simpler and faster than the existing analytical characterization technology. When lead ions are adsorbed onto the surface of the optical fiber, they change the refractive index of the optical fiber surface, which in turn causes a shift in the peak or a change in the intensity in the SPR spectrum (e.g. Figure 5 When a -1V potential is applied in a 20μM lead ion solution, the fiber with GB hydrogel attached shows a 6nm SPR wavelength shift, while the bare fiber only shows a 2.5nm shift in the lead ion solution, demonstrating the efficient adsorption and electrodeposition of lead ions by GB hydrogel, which leads to a significant change in the medium properties of the fiber surface. The presence of GB hydrogel on the fiber surface significantly enhances the electrosorption and electrodeposition process of lead ions.

[0040] In order to prove that the optical fiber attached with GB hydrogel has specific adsorption, various solutions (Na+, K+, Ca+, Mg+, Pb+) with a total cation concentration within 2μM were set according to the situation of blood lead poisoning. Then the hydrogel-coated optical fiber was immersed in the above solutions for incubation. A -1V potential was added to the solution to accelerate the speed of hydrogel adsorption of cations. The wavelength migration and current of each ion solution in its optical and electrochemical reactions were measured using optical fiber sensors. Figure 6 As shown in Figure 2, it was found that the specific adsorption capacity of GB hydrogel for lead ions can be further confirmed by optical or electrical methods, and optical fiber detection is highly sensitive to the use of GB hydrogel for lead ion purification. The signal of lead ions is significantly higher than that of other ions in both optical and electrochemical aspects; In order to prove that the optical fiber attached with GB hydrogel has the ability of adsorption-regeneration cycle, the optical fiber attached with GB hydrogel was immersed in 2μM lead ion solution for adsorption-regeneration cycle experiment. A -1V potential was added to the solution. After the adsorption was completed, the GB gel was used to release the adsorbed lead ions to achieve the cyclic adsorption of GB hydrogel. The process was repeated for more than 6 times, and the SPR spectrum and electrochemical signals of the adsorption-regeneration cycle were recorded by optical fiber sensor (such as Figure 7 as shown).

[0041] The above description is only a preferred embodiment of the present invention, and does not limit the patent scope of the present invention. All equivalent structural changes made by using the paper document and the contents of the drawings of the present invention under the inventive concept of the present invention, or direct / indirect application in other related technical fields are included in the patent protection scope of the present invention.

Claims

1. A method for preparing a blood perfusion diagnostic and therapeutic device based on guanosine supramolecular gel, characterized in that: The method comprises: preparing a guanosine-boric acid supramolecular hydrogel combined with TMPyP, wherein the guanosine-boric acid supramolecular hydrogel is a GB hydrogel; Then, the GB hydrogel combined with the TMPyP probe is attached to the surface of the U-shaped optical fiber; The optical fiber attached with GB hydrogel is immersed in a lead ion solution for adsorption. The lead ions compete with the sodium ions in the GB gel to form adsorption. EDTA sodium salt chelates the lead ions in the GB gel and regenerates and replaces them with sodium ions, thereby realizing the cyclic adsorption of GB hydrogel, that is, forming an intelligent blood perfusion device based on guanosine supramolecular gel.

2. The method for preparing a blood perfusion diagnostic and therapeutic device based on guanosine supramolecular gel according to claim 1, characterized in that: The TMPyP is α, β, γ, δ-tetrakis (1-methylpyridinium-4-yl) porphine p-toluenesulfonate.

3. The method for preparing a blood perfusion diagnostic and therapeutic device based on guanosine supramolecular gel according to claim 1, characterized in that: The U-shaped optical fiber is an optical fiber sensor based on surface plasmon resonance.

4. The method for preparing a blood perfusion diagnostic and therapeutic device based on guanosine supramolecular gel according to claim 1, characterized in that: The prepared perfusion device is used for the selective removal of heavy metal ions and can also realize real-time monitoring of toxic components in the blood.

5. The method for preparing a blood perfusion diagnostic and therapeutic device based on guanosine supramolecular gel according to claim 4, characterized in that: The prepared perfusion device is used for the selective removal of heavy metal ions and the real-time monitoring of toxic components in the blood.

6. The method for preparing a blood perfusion diagnostic and therapeutic device based on guanosine supramolecular gel according to claim 1, characterized in that: The preparation of the TMPyP-binding guanosine-boric acid supramolecular hydrogel comprises: To prepare 5 wt % GB hydrogel, 0.566 g guanosine and 0.056 g boric acid were mixed in 10 ml distilled water and shaken on a vortex mixer for 5 min; The mixture was heated in an oil bath at 95°C with magnetic stirring, and 2.5 ml of NaOH solution was added simultaneously; When the solution turned from turbid to clear, the heating was stopped and the solution was cooled to room temperature to obtain GB hydrogel.

7. The method for preparing a blood perfusion diagnostic and therapeutic device based on guanosine supramolecular gel according to claim 1, characterized in that: The optical fiber attached with GB hydrogel is immersed in a lead ion solution for adsorption, the lead ions in the solution compete with the sodium ions in the GB gel to form adsorption, EDTA sodium salt chelates the lead ions in the GB gel, and regenerates and replaces them with sodium ions, thereby realizing the cyclic adsorption of GB hydrogel, that is, forming an intelligent blood perfusion device based on guanosine supramolecular gel, including: Prepare a visualized regenerative and recyclable optical fiber device, and mix 150 μM TMPyP in the GB hydrogel preparation process to prepare a TMPyP-bound hydrogel; GB hydrogel with TMPyP probe is deposited on U-shaped optical fiber. TMPyP probe is used for qualitative and visual monitoring of blood lead. Optical fiber is used for optical / electrical dual-mode quantitative monitoring. The temperature of GB gel is raised to above 95°C to turn it into a solution state. Then the optical fiber is immersed in the GB gel solution. After a layer of gel is attached to the surface of the optical fiber, it is picked up and dried naturally. A U-shaped optical fiber with GB hydrogel attached was obtained by a dip-coating method. The optical fiber with GB hydrogel attached was immersed in a lead ion solution for adsorption. The lead ions competed with the sodium ions in the GB gel to form adsorption. EDTA sodium salt chelated the lead ions in the GB gel and regenerated them into sodium ions to achieve cyclic adsorption of GB hydrogel. The SPR spectrum and electrochemical signals of the adsorption-regeneration cycle were recorded by an optical fiber sensor, and finally an intelligent blood perfusion device based on GB hydrogel was prepared.

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