Carthamus tinctorius seed oil and wood frog egg oil nano-emulsion and preparation method thereof

Abstract

The invention discloses a safflower seed oil and wood frog egg oil nano-emulsion and a preparation method thereof. The safflower seed oil and wood frog egg oil nano-emulsion comprises the following components in parts by mass: 2-10 parts of compound oil, 2-6 parts of a surfactant, 0.5-4 parts of a cosolvent and 80-90 parts of deionized water, wherein the compound oil is composed of wood frog egg oil and safflower seed oil, and the mass ratio of the wood frog egg oil to the safflower seed oil in the compound oil is 1: 1-1: 4; the preparation method comprises the following steps: step 1, uniformly mixing safflower seed oil, wood frog egg oil, a surfactant and a cosolvent to obtain a first mixture; step 2, adding deionized water into the first mixture, and stirring to obtain crude emulsion; and step 3, crushing the crude emulsion by a cell crusher to obtain the safflower seed oil and wood frog egg oil nano-emulsion.

Description

Technical Field

[0001] The invention belongs to the technical field of application of forest frog egg oil, and particularly relates to a safflower seed oil forest frog egg oil nanoemulsion and a preparation method thereof. Background Art

[0002] The traditional precious Chinese medicine Rana oil is obtained by drying the solid oil near the oviduct of mature female frogs, and Rana eggs are the byproducts left after the collection of Rana oil. The oily substance obtained after impurity removal and extraction is called Rana egg oil. According to the GC-MS qualitative detection experiment of Rana egg oil conducted by Han Zihan, 34 kinds of fatty acids with a content greater than 1% in Rana egg oil were measured, of which saturated fatty acids accounted for 31.95% and unsaturated fatty acids accounted for 68.05%. The main fatty acid components are tetradecanoic acid, 9-hexadecenoic acid, hexadecanoic acid, 14-methylhexadecanoic acid, 15-methylhexadecanoic acid, linoleic acid, oleic acid, octadecanoic acid, nonadecanoic acid, EPA, 11, 14, 17-decatrienoic acid, DHA, and docosenoic acid. In addition to fatty acids, frog egg oil also contains a large amount of sex hormones, such as estradiol, progesterone, testosterone, etc. These ingredients make frog egg oil have the effects of anti-oxidation, lowering blood lipids, improving anxiety, protecting ovaries and regulating endocrine. In addition, it also has the effects of improving facial skin and removing spots. However, these ingredients will also make frog egg oil easily react with oxygen in the air, causing products with frog egg oil as the main ingredient to be unstable and easy to oxidize and discolor.

[0003] Safflower is a herbaceous plant of the genus Carthamus in the Asteraceae family. Safflower seed oil contains a large amount of fatty acid components including oleic acid, palmitic acid, stearic acid, linolenic acid and linoleic acid. Among them, the linoleic acid component in dry safflower seed oil can be as high as 73-85%, and it is known as the "king of linoleic acid". In addition to fatty acid components, safflower seed oil also contains sterols and flavonoids. It has the effects of improving blood lipids and anti-oxidation. Safflower seed oil is extremely rich in effective ingredients and nutrients, and is widely used in medicine, industrial raw materials, etc. Summary of the invention

[0004] The invention aims to provide a safflower seed oil and frog egg oil nanoemulsion, which is prepared by combining frog egg oil and safflower seed oil to form the nanoemulsion, can cover up the peculiar smell of frog egg oil, and has good anti-oxidation effect.

[0005] The invention also provides a method for preparing the safflower seed oil and frog egg oil nanoemulsion.

[0006] The technical solution provided by the present invention is:

[0007] A method for preparing a safflower seed oil and frog egg oil nanoemulsion, wherein the components of the safflower seed oil and frog egg oil nanoemulsion and the mass fractions of each component are as follows:

[0008] 2-10 parts of complex oil, 2-6 parts of surfactant, 0.5-4 parts of cosolvent, 80-90 parts of deionized water;

[0009] The compound oil is composed of frog egg oil and safflower seed oil, and the mass ratio of frog egg oil to safflower seed oil in the compound oil is 1:1 to 1:4.

[0010] Preferably, the surfactant is Tween 20, Tween 80, Span 20, Span 80 or EL-20.

[0011] Preferably, the cosolvent is n-butanol, glycerol, 1,2-propylene glycol, 1,3-propylene glycol or anhydrous ethanol.

[0012] A method for preparing a safflower seed oil and frog egg oil nanoemulsion, which is used to prepare the safflower seed oil and frog egg oil nanoemulsion, comprises the following steps:

[0013] Step 1: uniformly mixing safflower seed oil, Rana sylvatica egg oil, a surfactant and a cosolvent to obtain a first mixture;

[0014] Step 2: adding deionized water to the first mixture and stirring to obtain a crude emulsion;

[0015] Step 3: The crude emulsion is crushed by a cell crusher to obtain the safflower seed oil and frog egg oil nanoemulsion.

[0016] Preferably, in the step 2, stirring is performed by a magnetic stirrer, the stirring speed is 1000 r / min, and the stirring time is 40 to 60 min.

[0017] Preferably, in step three, the cell pulverizer is an ultrasonic cell pulverizer.

[0018] Preferably, the ultrasonic power of the cell crusher is 250W to 350W, and the ultrasonic time is 20 to 30 minutes.

[0019] The beneficial effects of the present invention are:

[0020] The safflower seed oil and frog egg oil nanoemulsion provided by the invention can cover up the peculiar smell of frog egg oil and has good anti-oxidation effect.

[0021] The method for preparing the safflower frog egg oil nanoemulsion provided by the present invention can make the particle size of the prepared nanoemulsion smaller, improve the coating effect of the nanoemulsion on the composite oil, and improve the stability of the nanoemulsion by reasonably setting the process parameters in the preparation process. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1This is a transmission electron microscope morphology image of the emulsion prepared in Example 1 of the present invention.

[0023] Figure 2 This is a transmission electron microscope morphology image of the emulsion prepared in Example 2 of the present invention.

[0024] Figure 3 Spectra of the nanoemulsion prepared in Example 1 of the present invention, Tween 80 and the nanoemulsion of Example 2.

[0025] Figure 4 The figure is a curve diagram of the scavenging rate of hydroxyl radicals by the diluted samples of the nanoemulsions prepared in various embodiments and comparative examples of the present invention.

[0026] Figure 5 The figure is a graph showing the scavenging rate of ABTS+ free radicals by the diluted samples of the nanoemulsions prepared in various embodiments and comparative examples of the present invention.

[0027] Figure 6 The figure is a graph showing the scavenging rate of DPPH free radicals by the diluted samples of the nanoemulsions prepared in various embodiments and comparative examples of the present invention. DETAILED DESCRIPTION

[0028] The present invention is further described in detail below in conjunction with the accompanying drawings so that those skilled in the art can implement the invention with reference to the description.

[0029] The invention provides a safflower frog egg oil nanoemulsion, wherein the components of the safflower frog egg oil nanoemulsion and the mass fractions of each component are: 2 to 10 parts of compound oil, 2 to 6 parts of surfactant, 0.5 to 4 parts of cosolvent, and 80 to 90 parts of deionized water. The compound oil is composed of safflower frog egg oil and safflower seed oil, and the mass ratio of safflower frog egg oil to safflower seed oil in the compound oil is 1:1 to 1:4.

[0030] The surfactant is Tween20, Tween80, Span20, Span80 or EL-20.

[0031] The cosolvent is n-butanol, glycerol, 1,2-propylene glycol, 1,3-propylene glycol or anhydrous ethanol.

[0032] The invention also provides a method for preparing the safari forest frog egg oil nanoemulsion, which is used to prepare the safari forest frog egg oil nanoemulsion. The specific preparation process is as follows.

[0033] Step 1: Evenly mix 2 to 10 parts of compound oil (safflower seed oil and Rana sylvatica egg oil), 2 to 6 parts of surfactant and 0.5 to 4 parts of co-solvent according to weight proportions to obtain a first mixture.

[0034] Wherein, the mass ratio of forest frog egg oil to safflower seed oil in the compound oil is 1:1 to 1:4.

[0035] Step 2: Add 80 to 90 parts by mass of deionized water to the first mixture, and stir to obtain a crude emulsion.

[0036] Step 3: The crude emulsion is crushed by a cell crusher to obtain the Rana chensinensis egg oil nanoemulsion.

[0037] As a preferred embodiment, in step 2, deionized water is added to the first mixture and then stirred by a magnetic stirrer at a stirring speed of 1000 r / min for a stirring time of 40 to 60 min.

[0038] As a preferred embodiment, in step 3, the cell pulverizer is an ultrasonic cell pulverizer and a 6 mm ultrasonic probe is used. The ultrasonic frequency of the cell pulverizer is 20-25 KHz.

[0039] Most preferably, the ultrasonic power of the cell crusher is 250W to 350W, and the ultrasonic time is 20 to 30 minutes.

[0040] The centrifugal stability of the prepared nano-solution can be improved by reasonably setting the stirring speed, stirring time, ultrasonic power and ultrasonic time.

[0041] The nanoemulsion prepared by the invention can inhibit the oxidation and deterioration of PUFAs caused by contact with air, retain the effective components of forest frog egg oil and safflower seed oil to the maximum extent, and has a good anti-oxidation effect.

[0042] Example 1

[0043] According to the weight ratio, 5 parts of compound oil (the weight ratio of Rana cynomys lindens egg oil to safflower seed oil is 1:1), 4 parts of Tween80 and 1 part of n-butanol were mixed evenly to obtain a first mixture.

[0044] According to the mass fraction, 90 parts of deionized water were slowly added to the first mixture, and stirred for 40 minutes with a magnetic stirrer at 1000 r / min to obtain a crude emulsion. The mixture was treated with an ultrasonic cell crusher at 300 W for 20 minutes to form a uniform emulsion, namely, the safflower frog egg oil nanoemulsion.

[0045] Example 2

[0046] According to the weight ratio, 5 parts of compound oil (the weight ratio of Rana cynomys lindens egg oil to safflower seed oil is 1:4), 4 parts of Tween80 and 1 part of glycerol are uniformly mixed to obtain a first mixture.

[0047] According to the mass fraction, 90 parts of deionized water were slowly added to the first mixture, and stirred for 40 minutes with a magnetic stirrer at 1000 r / min to obtain a crude emulsion. The mixture was treated with an ultrasonic cell crusher at 250 W for 20 minutes to form a uniform emulsion, which was the safflower frog egg oil nanoemulsion.

[0048] Example 3

[0049] According to the weight ratio, 5 parts of compound oil (the weight ratio of Rana sylvatica egg oil to safflower seed oil is 2:3), 4 parts of Span20 and 1 part of propylene glycol are uniformly mixed to obtain a first mixture.

[0050] According to the mass fraction, 90 parts of deionized water were slowly added to the first mixture, and stirred for 45 minutes with a magnetic stirrer at 1000 r / min to obtain a crude emulsion. The mixture was treated with an ultrasonic cell crusher at 330W for 30 minutes to form a uniform emulsion, which was the safflower seed oil and frog egg oil nanoemulsion.

[0051] Comparative Example 1

[0052] According to the weight ratio, 5 parts of Rana sylvatica egg oil, 4 parts of Tween80 and 1 part of propylene glycol were uniformly mixed to obtain a first mixture.

[0053] According to the mass fraction, 90 parts of deionized water were slowly added to the first mixture, and stirred for 40 minutes with a magnetic stirrer at 1000 r / min to obtain a crude emulsion. The mixture was treated with an ultrasonic cell crusher at 300 W for 20 minutes to form a uniform emulsion, namely, the forest frog egg oil nanoemulsion.

[0054] Comparative Example 2

[0055] According to the weight ratio, 5 parts of safflower seed oil, 4 parts of Tween80 and 1 part of glycerol were uniformly mixed to obtain a first mixture.

[0056] According to the mass fraction, 90 parts of deionized water were slowly added to the first mixture, and stirred for 40 minutes with a magnetic stirrer at 1000 r / min to obtain a crude emulsion. The mixture was treated with an ultrasonic cell crusher at 300 W for 20 minutes to form a uniform emulsion, namely, safflower seed oil nanoemulsion.

[0057] The quality standards of the raw materials used in Examples 1-3 are shown in Table 1:

[0058] Table 1

[0059] name standard Manufacturer or preparation method Rana oleifera egg oil Enterprise Standards Homemade (subcritical butane extraction of eggs and fascia) Safflower Oil National Standards Henan Kunhua Biological Co., Ltd. Span20 Chemically pure Shanghai Aladdin Biochemical Technology Co., Ltd. Tween80 Chemically pure Shanghai Aladdin Biochemical Technology Co., Ltd. n-Butanol Chemically pure Shanghai Aladdin Biochemical Technology Co., Ltd. C3-pure Chemically pure Shanghai Aladdin Biochemical Technology Co., Ltd.

[0060] The safflower seed oil and frog egg oil nanoemulsion prepared in Examples 1-3 was placed at room temperatures of 4°C, 25°C, -10°C, and -20°C for 7 days, 15 days, and 30 days, and the nanoemulsion was observed with the naked eye to see whether it was uniform and without stratification.

[0061] After 30 days of observation, the nanoemulsions prepared in Examples 1-3 were still uniform without stratification, precipitation, or demulsification, indicating that the nanoemulsions prepared in Examples 1-3 had high temperature stability.

[0062] The safflower seed oil and frog egg oil nanoemulsion prepared in Example 1-3 was diluted 50 times to avoid the phenomenon of multiple scattered colors formed by the emulsion with higher concentration, which would affect the results. The average particle size, polydispersity index PDI and average ZeTa potential value of the nanoemulsion were detected using a Malvern laser particle size analyzer.

[0063] The test results are shown in Table 2.

[0064] Table 2 Particle size, PDI and Zeta potential value results

[0065]

[0066] From the test results, it can be seen that the particle size of the nanoemulsion prepared in Example 1 is smaller than that of the nanoemulsion prepared in Example 2, and the absolute value of the Zeta potential is greater than that of the nanoemulsion prepared in Example 2, indicating that the nanoemulsion prepared in Example 1 has a small particle size and high stability. However, the PDI value of the nanoemulsion prepared in Example 2 is smaller than that of Example 1, indicating that the uniformity of the nanoemulsion prepared in Example 2 is higher than that of Example 1. The particle size of Example 3 is larger, and the dispersion uniformity is between Examples 1 and 2.

[0067] like Figure 1-2 As shown, the nanoemulsions prepared in Example 1 and Example 2 were respectively detected by transmission electron microscopy, and it was found that the particle size of Example 1 was smaller than that of the nanoemulsion prepared in Example 2, which was consistent with the results of Malvern laser particle size analyzer. The droplets of the nanoemulsions prepared in Example 1 and Example 2 were both round and had good droplet dispersion.

[0068] The embedding effect of the nanoemulsion prepared in Example 1 and Example 2 was tested, and the results are as follows: Figure 3 shown.

[0069] The complex oil in the nanoemulsion prepared in Example 1 is at 3008 cm -1 The infrared absorption peak at 2926 cm-1 belongs to the CH stretching vibration of olefins. -1 The infrared absorption peak at 2855 cm-1 belongs to the antisymmetric stretching vibration of alkane CH. -1 The infrared absorption peak at 1743 cm is attributed to the symmetric stretching vibration of alkane CH. -1The infrared absorption peak at 1709cm is attributed to C=O stretching vibration. -1 The infrared absorption peak at 1461 cm is attributed to C=C stretching vibration. -1 The infrared absorption peak at 1163cm belongs to the CH bending vibration of alkanes. -1 、1099cm -1 、1047cm -1 The infrared absorption peak at 722 cm belongs to COC stretching vibration. -1 The infrared absorption peak at 2926cm -1 、2855cm -1 、1743cm -1 In the infrared spectrum of the nanoemulsion prepared in Example 1, the characteristic peaks of the complex oil are obviously weakened or even disappear. In the infrared spectrum of the nanoemulsion prepared in Example 1, the OH stretching vibration peak has been reduced from 3320 cm -1 Offset to 3410cm -1 This indicates that the hydrogen bonding between Tween 80 and the composite oil is enhanced, indicating that the nanoemulsion prepared in Example 1 is successful in encapsulating the composite oil-1.

[0070] The complex oil in the nanoemulsion prepared in Example 2 is at 3008 cm -1 The infrared absorption peak at 2926 cm-1 belongs to the CH stretching vibration of olefins. -1 The infrared absorption peak at 2855 cm-1 belongs to the antisymmetric stretching vibration of alkane CH. -1 The infrared absorption peak at 1743 cm is attributed to the symmetric stretching vibration of alkane CH. -1 The infrared absorption peak at 1709cm is attributed to C=O stretching vibration. -1 The infrared absorption peak at 1461 cm is attributed to C=C stretching vibration. -1 The infrared absorption peak at 1163cm belongs to the CH bending vibration of alkanes. -1 、1099cm -1 、1047cm -1 The infrared absorption peak at 722 cm belongs to COC stretching vibration. -1 The infrared absorption peak at 3482cm belongs to the in-plane swing of CH2. -1 、2926cm -1 、2855cm -1 、1743cm -1The wavelengths at 2363 cm-1 belong to the OH stretching vibration of hydrogen bonds between hydroxyl molecules, the CH antisymmetric stretching vibration of alkanes, the CH symmetric stretching vibration of alkanes, and the C=O stretching vibration. -1 The characteristic peak of the composite oil is obviously weakened or even disappears. In the infrared spectrum of the nanoemulsion prepared in Example 2, the OH stretching vibration peak has increased from 3320 cm -1 Offset to 3370cm -1 , indicating that the hydrogen bonding effect between Tween 80 and the composite oil is enhanced, indicating that the nanoemulsion prepared in Example 2 is successful in encapsulating the composite oil.

[0071] The nanoemulsions prepared in Examples 1-3, Comparative Examples 1-2 and the positive control vitamin E (VE) were tested for antioxidant activity. The test results are as follows: Figure 4-6 shown.

[0072] (1) Test on scavenging rate of hydroxyl free radicals (the samples used in the test were the nanoemulsions prepared in the examples and comparative examples and the positive control VE, which were diluted with anhydrous ethanol to a concentration of 10 mg·mL of oil or VE. -1 , 15mg·mL -1 , 20mg·mL -1 , 25mg·mL -1 、30mg·mL -1 .

[0073] Take three 25mL test tubes, namely sample tube, blank tube and sample background tube, and add 5mL 1mmol·L -1 Ferrous sulfate solution, 5mL 13mmol·L -1 Hydrogen peroxide, add 1mL of sample solution and then add 3mmol·L -1 Salicylic acid solution was diluted to volume. 5 mL of 1 mmol·L was added to the blank tube. -1 Ferrous sulfate solution, 5mL 13mmol·L -1 Hydrogen peroxide, add 1mL of distilled water and then add 3mmol·L -1 Add 5 mL of 1 mmol·L salicylic acid solution to the sample base tube. -1 Add 1 mL of sample solution to 5 mL of distilled water and then 3 mmol·L of ferrous sulfate solution. -1 The salicylic acid solution is diluted to the mark. After reacting in constant temperature water at 37°C for 15 minutes, the absorbance is measured at a wavelength of 510 nm using a spectrophotometer. The blank tube is A0, the sample tube is A1, and the background color tube is A2. Repeat three times, calculate the results, and draw a clearance curve.

[0074] Hydroxyl radical scavenging rate (%) = [1-(A1-A2) / A0] × 100

[0075] The results are as follows Figure 4 As shown in the figure, vitamin E as a positive control, three different proportions of compound oil, frog egg oil and safflower seed oil have the ability to scavenge hydroxyl free radicals, and there is a certain dose-effect relationship. As the concentration of frog egg oil and safflower seed oil increases, the hydroxyl free radical scavenging rate also increases steadily. When the concentrations of frog egg oil and safflower seed oil are 30 mg·mL -1 The scavenging rates of the two are 87.95% and 90.36% respectively, and the scavenging rates of the two are similar, and safflower seed oil is slightly higher than that of wood frog egg oil. As can be seen from Figure 4, the scavenging ability of the nanoemulsions prepared in Examples 1-3 on hydroxyl radicals is higher than that in Comparative Example 1 and Comparative Example 2, and the nanoemulsion prepared in Example 1 (complex oil (1:1)) has the highest scavenging rate of hydroxyl radicals among the five nanoemulsions, indicating that its in vitro antioxidant capacity is the highest.

[0076] (2) ABTS+ free radical scavenging rate test (the samples used in the test were the nanoemulsion samples prepared in each embodiment and comparative example and the positive control VE, which were diluted with anhydrous ethanol to a fat or VE concentration of 10 mg mL -1 , 20mg·mL -1 、30mg·mL -1 , 40mg·mL -1 , 50mg·mL -1 .

[0077] Take 7.4mmol·L -1 20 mL of [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] (ABTS) stock solution and 2.6 mmol·L -1 20 mL of potassium persulfate stock solution was mixed and reacted at room temperature in the dark for 12-16 h, and diluted with anhydrous ethanol until the absorbance of the solution reached 0.70 ± 0.02 to prepare ABTS + Working solution. Take 1.6mL of ABTS+ working solution and 0.4mL of sample solution of different concentrations and mix them evenly. Keep it away from light and let it react for 6 minutes. Measure the absorbance A1 at a wavelength of 734nm. Take 1.6mL of ABTS+ working solution and add 0.4mL of anhydrous ethanol solution and mix them evenly. Keep it away from light and let it react for 6 minutes. Measure the absorbance A2 at a wavelength of 734nm. Repeat three times, calculate the results, and draw the clearance curve.

[0078] ABTS+ clearance rate (%) = [(A2-A1) / A2] × 100

[0079] The fruit Figure 5As shown in the figure, the scavenging ability of vitamin E as a positive control, three different proportions of re-oil, frog egg oil and safflower seed oil on ABTS+ free radicals is positively correlated with the sample concentration. The scavenging ability of frog egg oil and safflower seed oil on ABTS+ free radicals is generally lower than that of VE. -1 To 50mg·mL -1 The ABTS+ free radical scavenging rate had almost no increase and was stable at around 94.00%. The overall ABTS+ free radical scavenging capacity of safflower seed oil was higher than that of Rana sylvatica egg oil, both of which were at 30 mg·mL -1 To 50mg·mL -1 The clearance rate increased fastest when the concentration reached 50 mg·mL -1 The clearance rates of Rana foetida egg oil and safflower seed oil were 69.04% and 79.41% respectively. It can be seen from the figure that among the five oils, Rana foetida egg oil has the lowest in vitro antioxidant capacity by ABTS+ method, and the compound oil (1:1) has the highest in vitro antioxidant capacity by ABTS+ method.

[0080] (3) DPPH free radical scavenging rate test (the samples used in the test were the nanoemulsions prepared in each embodiment and comparative example and the positive control VE, which were diluted with anhydrous ethanol to a fat or VE concentration of 10 mg mL -1 , 15mg·mL -1 , 20mg·mL -1 , 25mg·mL -1 、30mg·mL -1 .

[0081] Take a certain amount of 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) and prepare it with anhydrous ethanol to a concentration of 0.2mmol·L -1 Solution. Vitamin E (VE) was used as a positive control. 4 mL of sample and 4 mL of DPPH solution were added to a test tube and mixed, and allowed to stand for 30 minutes in the dark. The absorbance value A1 was measured at 517 nm using a UV spectrophotometer. 4 mL of DPPH solution and 4 mL of anhydrous ethanol were added to a test tube and mixed, and allowed to stand for 30 minutes in the dark. The absorbance value A2 was measured at 517 nm using a UV spectrophotometer. 4 mL of distilled water and 4 mL of anhydrous ethanol were added to a test tube and mixed, and allowed to stand for 30 minutes in the dark. The absorbance value A3 was measured at 517 nm using a UV spectrophotometer. Repeat three times, calculate the results, and draw a clearance curve.

[0082] DPPH free radical scavenging rate (%) = [1 – (A1 – A3) / A2] × 100

[0083] The results are as follows Figure 6As shown in the figure, DPPH free radicals are relatively stable and can be easily reduced by antioxidants. The antioxidant capacity of the three oils, frog egg oil and safflower seed oil in vitro increases with the increase of concentration. VE concentration is 10 mg mL -1 Its clearance rate can reach 89.97% when the concentration is 25 mg·mL, and it has been stable at around 90% with the increase of concentration. -1 To 30mg·mL -1 The scavenging capacity increased rapidly, and the overall scavenging rate of Rana oleifera egg oil was slightly higher than that of safflower seed oil. The results of the three composite oils showed that the DPPH antioxidant activity of the composite oil (1:1) was the highest, and the antioxidant capacity of the composite oil was greater than that of a single oil.

[0084] The present invention combines forest frog egg oil and safflower seed oil to form a nanoemulsion, so that the composite oil can be wrapped in the nanoemulsion, thereby covering the fishy smell and color of the forest frog egg oil. At the same time, due to the coating effect of the nanoemulsion, the polyunsaturated fatty acids in the composite oil can be prevented from contacting the air, thereby inhibiting the oxidation and deterioration of PUFAs in the composite oil, improving the bioavailability of PUFAs, and then enhancing the anti-oxidation effect of the nanoemulsion. After being emulsified into a nanoparticle size, the specific surface area and penetration ability of the oil droplets are also increased, which is more conducive to exerting the anti-oxidation effect of the unsaturated fatty acid components. The forest frog egg oil derived from animals and the safflower seed oil derived from plants just contain complementary contents of two types of unsaturated fatty acids, ω-3 and ω-6, and the reasonable compatibility of the two has better activity than using them alone. It has been verified that the nanoemulsion of wood frog egg oil and safflower seed oil prepared by the present invention has better antioxidant effect than the nanoemulsion prepared by using wood frog egg oil alone (the content of the composite oil in the nanoemulsion is the same) and the nanoemulsion prepared by using safflower seed oil alone (the content of the composite oil in the nanoemulsion is the same).

[0085] The safflower frog egg oil nanoemulsion prepared by the invention can be used in the fields of medicine, health food and cosmetics, for example, to prepare oral or external emulsions, soft capsules, gel candies and the like.

[0086] Although the embodiments of the present invention have been disclosed as above, they are not limited to the applications listed in the specification and the implementation modes, and they can be fully applied to various fields suitable for the present invention. For those familiar with the art, additional modifications can be easily implemented. Therefore, without departing from the general concept defined by the claims and the scope of equivalents, the present invention is not limited to the specific details and the illustrations shown and described herein.

Claims

1. A safflower seed oil and frog egg oil nanoemulsion, characterized in that: The components of the safflower seed oil frog egg oil nanoemulsion and the mass fractions of each component are as follows: 2-10 parts of complex oil, 2-6 parts of surfactant, 0.5-4 parts of cosolvent, 80-90 parts of deionized water; The compound oil is composed of frog egg oil and safflower seed oil, and the mass ratio of frog egg oil to safflower seed oil in the compound oil is 1:1 to 1:

4.

2. The safflower seed oil and frog egg oil nanoemulsion according to claim 1, characterized in that The surfactant is Tween20, Tween80, Span20, Span80 or EL-20.

3. The preparation method of the safflower oil frog egg oil nanoemulsion according to claim 1 or 2, characterized in that, The cosolvent is n-butanol, glycerol, 1,2-propylene glycol, 1,3-propylene glycol or anhydrous ethanol.

4. A method for preparing a safflower seed oil and frog egg oil nanoemulsion, for preparing the safflower seed oil and frog egg oil nanoemulsion as claimed in any one of claims 1 to 3, characterized in that: The steps include: Step 1: uniformly mixing safflower seed oil, Rana sylvatica egg oil, a surfactant and a cosolvent to obtain a first mixture; Step 2: adding deionized water to the first mixture and stirring to obtain a crude emulsion; Step 3: The crude emulsion is crushed by a cell crusher to obtain the safflower seed oil and frog egg oil nanoemulsion.

5. The method for preparing the safflower oil frog egg oil nanoemulsion according to claim 4, characterized in that: In the step 2, stirring is performed by a magnetic stirrer at a stirring speed of 1000 r / min and a stirring time of 40 to 60 min.

6. The method for preparing the safflower seed oil and frog egg oil nanoemulsion according to claim 5, characterized in that: In the step three, the cell crusher is an ultrasonic cell crusher.

7. The method for preparing the safflower oil frog egg oil nanoemulsion according to claim 6, characterized in that: The ultrasonic power of the cell crusher is 250W to 350W, and the ultrasonic time is 20 to 30 minutes.

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