Taq ManMGB probe for detecting matrilinear inheritance chondriosome deafness gene C1494T mutation

A deafness gene, mitochondrial technology, applied in the field of genetic engineering, can solve the problems of high cost, long time, cumbersome steps, etc.

Active Publication Date: 2007-06-27
金政策
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Problems solved by technology

[0004] Since the discovery of the relationship between mtDNA C1494T and deafness in 2004, the detection of this mutation mainly uses the method of direct sequencing. Although sequence determination is an effective method for evaluating gene mutations, it is costly, cumbersome, and time-consuming. To solve these problems, we A simple, rapid, accurate, contamination-reducing, and inexpensive assay was designed to meet the need for widespread screening of the mtDNA C1494T mutation

Method used

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  • Taq ManMGB probe for detecting matrilinear inheritance chondriosome deafness gene C1494T mutation

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Embodiment Construction

[0043] Detection of C1494T Mutation in Mitochondrial Gene of Maternally Inherited Deafness

[0044] 1. Test samples

[0045] 72 patients with sensorineural deafness were selected from the Deaf Disease Resource Bank of the Deaf Disease Molecular Diagnosis Center of the General Hospital of the Chinese People's Liberation Army, and peripheral whole blood DNA was extracted from the tested individuals (Yuan Huijun et al., Chinese Journal of Otorhinolaryngology, 1998, 33(2) : 67-70; Li Weimin et al., Journal of Clinical Otorhinolaryngology, 2001, 15 (Supplement): 53-58), as a test sample.

[0046] 2. MGB probe and primer design

[0047] According to the published mitochondrial gene sequence (Cambridge Sequence or NCBI human mitochondrial genome sequence NC-001807.4 or NT-006713.14, etc., or sequence number SEQ ID NO: 5), use the Genetool Lite program to assist in the design of primers and Taqman MGB probe sequences, wherein :

[0048] The nucleotide sequence of the mutant MGB pro...

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Abstract

The invention designs two pieces of Taqman mutant type and wild type MGB probe and a pair of primer. Using real time quantitative Taqman MGB probe method carries out genotype analysis for C1494T mutation of deaf gene of maternal inheritance mitochondria so as to diagnose genetic deaf disease of maternal mitochondria. The method is suitable to large-scale screening or preventative inspecting C1494T mutation of deaf mitochondria gene of maternal inheritance. Features are: simple, time saving, high specificity, high sensitivity, intuitive tested result, accurate and reliable.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a probe for diagnosing maternal mitochondrial genetic deafness and its application, more specifically to a real-time quantitative TaqMan MGB probe for detecting the mutation of maternal mitochondrial deafness gene C1494T and its application. The present invention also relates to the application of the MGB probe and its related products in the preparation of kits or similar products for diagnosing maternally inherited mitochondrial deafness. Background technique [0002] Aminoglycoside antibiotics (streptomycin, gentamycin, kanamycin, tobramycin and micronomycin, etc.) are widely used clinically because of their broad-spectrum and high-efficiency antibacterial effects and low prices. Controls Gram-negative and positive bacterial infections, but these antibiotics have serious ototoxic side effects that can cause irreversible hearing loss in patients. Studies in the pas...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/52C12Q1/68C12N15/11
CPCC12Q2600/156C12Q1/6883C12N15/11G01N33/52
Inventor 戴朴袁永一韩东一金政策
Owner 金政策
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