Pipetting dilution instrument

Abstract

The utility model relates to a pipetting dilution instrument which comprises a liquid storage bottle, a first three-way valve, a second three-way valve, a small-volume pipetting assembly, a large-volume pipetting assembly, a needle tube assembly and a cleaning block. According to the pipetting dilution instrument, two hands of a laboratory technician are liberated, sample dilution and needle tube and pipeline cleaning are carried out through the instrument, the experiment efficiency is improved, the experiment is standardized and processed, the instrument is more accurate, labor and time are saved, the result is more accurate, the labor is saved, the injury and fatigue of the personnel are avoided, and the working efficiency is improved. The dilution result is more accurate, errors are reduced, the experiment success rate is guaranteed, and experiment refinement is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of liquid transfer and dilution, in particular to a liquid transfer and dilution instrument. Background Art

[0002] When testing samples in an experiment, if the sample concentration is higher than the sensitivity of the testing instrument, dilution is required. Traditional experiments rely on manual work, and the experimenter needs to repeat many times, take liquids, and adjust the volume.

[0003] The existing problems are: 1. The experimental workload is large; 2. Fatigue of the experimenters and poor repeatability can easily lead to inaccurate experiments, requiring re-sampling experiments, but the pre-treatment of many samples is relatively complicated; 3. Repeated operations are a test of the experimenters' physical strength; 4. Improper operations may cause large errors in the experiment; 5. The cleaning task after the experiment is completed is heavy.

[0004] Therefore, a pipetting diluter is designed to solve the above problems. Summary of the invention

[0005] In order to overcome the above shortcomings, the utility model provides a liquid diluting instrument, including a liquid storage bottle, a No. 1 three-way valve, a No. 2 three-way valve, a small volume liquid transfer component, a large volume liquid transfer component, a needle tube component and a cleaning block;

[0006] The small volume pipetting assembly comprises a No. 1 screw motor and a No. 1 guide rail, wherein the No. 1 screw motor output shaft is connected to the No. 1 screw, the No. 1 screw is provided with a No. 1 slider, the No. 1 slider slides on the No. 1 guide rail, and the No. 1 slider is connected to a row of small syringes arranged in parallel; the No. 1 slider moves to drive the piston movement of the small syringe to complete suction and injection;

[0007] The large volume liquid transfer assembly comprises a No. 2 lead screw motor and a No. 2 guide rail, wherein the No. 2 lead screw motor output shaft is connected to the No. 2 lead screw, the No. 2 lead screw is provided with a No. 2 slider, the No. 2 slider slides on the No. 2 guide rail, and the No. 2 slider is connected to a row of large syringes arranged in parallel; the movement of the No. 2 slider can drive the piston movement of the large syringe to complete suction and injection;

[0008] The needle tube assembly comprises a horizontal linear reciprocating mechanism and a vertical linear reciprocating mechanism, the vertical linear reciprocating mechanism is provided with a connecting rod, and a row of needle tubes are arranged side by side on the connecting rod;

[0009] The liquid storage bottle is filled with liquid by connecting to a liquid filling pump, and the liquid storage bottle is respectively connected to the first channel in the No. 1 three-way valve and the first channel in the No. 2 three-way valve through pipelines; the second channel of the No. 1 three-way valve is connected to the needle tube, and the third channel of the No. 1 three-way valve is connected to the small syringe; the second channel of the No. 2 three-way valve is connected to the needle tube, and the third channel of the No. 2 three-way valve is connected to the large syringe.

[0010] Preferably, the pipetting diluter further includes a shell, and the liquid storage bottle, the dilution test tube for the experiment, the sample tube for the experiment, the cleaning block, the connecting rod and the needle tube are placed on the top shell of the shell; the small volume pipetting assembly, the large volume pipetting assembly and the horizontal linear reciprocating mechanism are placed in the shell.

[0011] Preferably, the small-volume pipetting assembly is supported and connected to the housing through a No. 1 bottom plate; and the large-volume pipetting assembly is supported and connected to the housing through a No. 2 bottom plate.

[0012] Preferably, the horizontal linear reciprocating mechanism and the vertical linear reciprocating mechanism are supported and connected to the shell through a fixing plate.

[0013] Preferably, the top surface of the cleaning block is provided with a plurality of cleaning pools, each of which is provided with a liquid storage pool side by side, the cleaning pool and the top surface of the liquid storage pool are connected through an overflow groove; the bottom of the liquid storage pool is connected to a drainage channel, and the drainage channel is used to remove excess liquid.

[0014] Preferably, the vertical linear reciprocating mechanism includes a No. 3 screw motor and a No. 3 guide rail. The output shaft of the No. 2 screw motor is connected to the No. 3 screw. The No. 3 screw is provided with a No. 3 slider. The No. 3 slider slides on the No. 3 guide rail. The No. 3 slider of the vertical linear reciprocating mechanism is connected to a connecting rod.

[0015] Preferably, the No. 1 three-way valve and the No. 2 three-way valve are solenoid valves.

[0016] Preferably, the pipetting diluter is equipped with a dilution test tube rack and a sample tube rack.

[0017] The beneficial effects of the utility model are:

[0018] The liquid transfer diluter described in the utility model liberates the hands of the experimenter, and batch dilution of samples and cleaning of syringes and pipelines are carried out through the instrument, thereby improving the efficiency of the experiment, standardizing the experiment into a process, and handing it over to the instrument is more accurate and labor-saving and time-saving, and the result is more accurate, and it saves personnel labor, avoids personnel injury and fatigue, and the result is more accurate, reduces errors and mistakes, ensures the success rate of the experiment, and improves the refinement of the experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 The three-dimensional Figure 1 ;

[0020] Figure 2 It is the front view of the utility model;

[0021] Figure 3 The three-dimensional Figure 2(remove the front cover);

[0022] Figure 4 The three-dimensional Figure 3 (remove the front cover, top cover and side covers);

[0023] Figure 5 Stereoscopic small volume pipetting components Figure 1 ;

[0024] Figure 6 Stereoscopic small volume pipetting components Figure 2 ;

[0025] Figure 7 Stereoscopic large volume pipetting components Figure 1 ;

[0026] Figure 8 Stereoscopic large volume pipetting components Figure 2 ;

[0027] Fig. 9 This is a schematic diagram of the injection pipeline connection;

[0028] Fig.10 A three-dimensional needle tube assembly Figure 1 ;

[0029] Fig.11 A three-dimensional needle tube assembly Figure 2 ;

[0030] Fig.12 It is a schematic diagram of the three-dimensional structure of the cleaning block;

[0031] Fig.13 is a top view of the cleaning block;

[0032] Fig.14 for Fig.13 AA cross-section diagram;

[0033] in:

[0034] 1-shell; 2-liquid storage bottle; 3-dilution test tube; 4-connecting rod; 5-needle tube; 6-sample tube; 7-cleaning block; 8-No. 2 screw motor; 9-No. 1 screw motor; 10-liquid adding pump; 11-No. 1 bottom plate; 12-No. 1 guide rail; 13-small syringe; 14-No. 1 screw; 15-No. 1 slider; 16-fixed plate; 17-No. 2 guide rail; 18-No. 2 screw; 19-large syringe; 20-No. 2 slider; 21-horizontal linear reciprocating mechanism; 22-vertical linear reciprocating mechanism; 23-No. 1 three-way valve; 24-No. 2 three-way valve; 25-cleaning tank; 26-liquid storage tank; 27-water outlet channel; 28-overflow tank; DETAILED DESCRIPTION

[0035] The following is a detailed description of the embodiments of the present invention with reference to the accompanying drawings.

[0036] Example

[0037] A pipetting diluter, comprising a liquid storage bottle 2, a No. 1 three-way valve 23, a No. 2 three-way valve 24, a small volume pipetting component, a large volume pipetting component, a needle tube component and a cleaning block;

[0038] The pipetting diluter also includes a shell 1, the liquid storage bottle 2, the dilution test tube 3 for the experiment, the sample tube 6 for the experiment, and the cleaning block 7 are placed on the top shell of the shell 1; the small volume pipetting component, the large volume pipetting component and the linear reciprocating mechanism 21 are placed in the shell 1.

[0039] The small volume pipetting assembly includes a No. 1 screw motor 9 and a No. 1 guide rail 12, the output shaft of the No. 1 screw motor 9 is connected to a No. 1 screw 14, a No. 1 slider 15 is arranged on the No. 1 screw 14, the No. 1 slider 15 slides on the No. 1 guide rail 12, and a row of small syringes 13 arranged in parallel are connected to the No. 1 slider 15; the movement of the No. 1 slider 15 can drive the piston movement of the small syringe 13 to complete suction and injection; the suction volume of the small syringe 13 at one time is the sample volume required for dilution;

[0040] The large volume pipetting assembly includes a No. 2 lead screw motor 8 and a No. 2 guide rail 17, the output shaft of the No. 2 lead screw motor 8 is connected to the No. 2 lead screw 18, the No. 2 lead screw 18 is provided with a No. 2 slider 20, the No. 2 slider 20 slides on the No. 2 guide rail 17, and a row of large syringes 19 arranged in parallel are connected to the No. 2 slider 20; the movement of the No. 2 slider 20 can drive the piston movement of the large syringe 19 to complete the suction and injection; the suction volume of the large syringe 19 is the volume required for sample dilution;

[0041] The needle tube assembly includes a horizontal linear reciprocating mechanism 21 and a vertical linear reciprocating mechanism 22, the vertical linear reciprocating mechanism 22 is provided with a connecting rod 4, and a row of needle tubes 5 are arranged side by side on the connecting rod 4; the connecting rod 4 and the needle tubes 5 are placed on the top shell of the housing 1;

[0042] The vertical linear reciprocating mechanism 22 includes a No. 3 screw motor and a No. 3 guide rail. The No. 2 screw motor output shaft is connected to the No. 3 screw. The No. 3 screw is provided with a No. 3 slider. The No. 3 slider slides on the No. 3 guide rail. The No. 3 slider of the vertical linear reciprocating mechanism 22 is connected to the connecting rod 4.

[0043] The liquid storage bottle 2 is filled with liquid by connecting to the liquid filling pump 10, and the liquid storage bottle 2 is respectively connected to the first channel in the No. 1 three-way valve 23 and the first channel in the No. 2 three-way valve 24 through pipelines; the second channel of the No. 1 three-way valve 23 is connected to the needle tube 5, and the third channel of the No. 1 three-way valve 23 is connected to the small syringe 13; the second channel of the No. 2 three-way valve 24 is connected to the needle tube 5, and the third channel of the No. 2 three-way valve 24 is connected to the large syringe 19.

[0044] The No. 1 three-way valve 23 and the No. 2 three-way valve 24 are solenoid valves.

[0045] The small volume pipetting assembly is supported and connected to the housing 1 through the first bottom plate 11 ; the large volume pipetting assembly is supported and connected to the housing 1 through the second bottom plate 16 .

[0046] The horizontal linear reciprocating mechanism 21 is supported and connected to the housing 1 via a fixing plate 22 .

[0047] A plurality of cleaning pools 25 are provided on the top surface of the cleaning block 7, each of which is provided with a liquid storage pool 26 side by side, and the top surfaces of the cleaning pool 25 and the liquid storage pool 26 are connected through an overflow groove 28; the bottom of the liquid storage pool 26 is connected to a drainage channel 27, and the drainage channel 27 is used to discharge excess liquid.

[0048] The liquid transfer diluter is equipped with a dilution test tube rack and a sample tube rack.

[0049] Directions:

[0050] The sample is in the sample tube, and it is necessary to know clearly how many times the sample has been diluted; the liquid storage bottle 2 is connected to the liquid adding pump 10 to add liquid;

[0051] 1. Pipette the sample to be diluted from the sample tube

[0052] The No. 1 three-way valve 23 connects the needle tube 5 and the small syringe 13, the No. 1 lead screw motor 8 drives the No. 1 slider 20 to move downward along the No. 1 guide rail 12, and the No. 1 slider 20 drives the small syringe 13 to complete the action of sucking liquid from the sample tube 6;

[0053] 2. Inject the sample and diluent into the dilution tube to complete the dilution

[0054] The No. 2 three-way valve 24 connects the passage between the liquid storage bottle 2 and the large syringe 19, and the No. 2 lead screw motor 8 drives the No. 2 slider 20 to move along the No. 2 guide rail 17, and the No. 2 slider 20 drives the large syringe 19 to complete the action of sucking the liquid in the liquid storage bottle 2.

[0055] The No. 2 three-way valve 24 connects the needle tube 5 and the large syringe 19, and the No. 2 lead screw motor 8 drives the No. 2 slider 20 to move along the No. 2 guide rail 17, and the No. 2 slider 20 drives the large syringe 19 to release the liquid into the dilution tube 3;

[0056] 3. Adjustment of the horizontal and vertical position of the needle tube 5

[0057] In order to accommodate large-scale operations, the sample tubes 6 are tested simultaneously in rows and transferred to the dilution tubes 3 in the row;

[0058] The movement of the horizontal position of each row, as well as the penetration into or removal from the sample tube 6 (or the dilution test tube 3), requires the height of the needle tube 5 to be adjusted, and the adjustment is completed by the needle tube assembly;

[0059] The horizontal linear reciprocating mechanism 21 is used to move the needle tube 5 horizontally, and the vertical linear reciprocating mechanism 22 is used to move the needle tube 5 vertically;

[0060] 4. Clean the needle 5

[0061] The horizontal linear reciprocating mechanism 21 is started to move the needle tube 5 to the position of the cleaning block 7, and the vertical linear reciprocating mechanism moves the needle tube 5 downward to insert it into the cleaning tank 25 of the cleaning block 7. The lubricating liquid is discharged from the liquid storage bottle 2 into the cleaning tank 25 through the small volume pipetting component or the large volume pipetting component to achieve cleaning of the inside and outside of the needle tube 5. After the liquid in the cleaning tank 25 is full, it will flow into the liquid storage tank 26 through the overflow groove 28 next to it, and the liquid storage tank 26 will discharge excess liquid through the outlet channel 27.

[0062] In the description of the present invention, it should be understood that the terms "upper", "lower", "front", "back", "left", "right", "top", "bottom", "inside", "outside", etc., indicating directions or positional relationships, are based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore should not be understood as a limitation on the present invention.

[0063] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood by specific circumstances.

[0064] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A pipetting diluter, characterized in that: It comprises a liquid storage bottle (2), a No. 1 three-way valve (23), a No. 2 three-way valve (24), a small volume liquid transfer component, a large volume liquid transfer component, a needle tube component and a cleaning block; The small volume liquid transfer assembly comprises a No. 1 screw motor (9) and a No. 1 guide rail (12); the output shaft of the No. 1 screw motor (9) is connected to a No. 1 screw (14); a No. 1 slider (15) is arranged on the No. 1 screw (14); the No. 1 slider (15) slides on the No. 1 guide rail (12); a row of small syringes (13) arranged in parallel are connected to the No. 1 slider (15); the movement of the No. 1 slider (15) can drive the piston movement of the small syringe (13) to complete suction and injection; The large volume liquid transfer assembly comprises a No. 2 screw motor (8) and a No. 2 guide rail (17); the output shaft of the No. 2 screw motor (8) is connected to the No. 2 screw (18); the No. 2 screw (18) is provided with a No. 2 slider (20); the No. 2 slider (20) slides on the No. 2 guide rail (17); the No. 2 slider (20) is connected to a row of large syringes (19) arranged in parallel; the movement of the No. 2 slider (20) can drive the piston movement of the large syringe (19) to complete suction and injection; The needle tube assembly comprises a horizontal linear reciprocating mechanism (21) and a vertical linear reciprocating mechanism (22); a connecting rod (4) is arranged on the vertical linear reciprocating mechanism (22); and a row of needle tubes (5) are arranged side by side on the connecting rod (4); The liquid storage bottle (2) is connected to a liquid adding pump (10) to add liquid. The liquid storage bottle (2) is respectively connected to a first channel in a No. 1 three-way valve (23) and a first channel in a No. 2 three-way valve (24) through pipelines; the second channel of the No. 1 three-way valve (23) is connected to a needle tube (5), and the third channel of the No. 1 three-way valve (23) is connected to a small-sized syringe (13); the second channel of the No. 2 three-way valve (24) is connected to the needle tube (5), and the third channel of the No. 2 three-way valve (24) is connected to a large-sized syringe (19).

2. A pipetting diluter according to claim 1, characterized in that: The liquid transfer diluter also comprises a housing (1); the liquid storage bottle (2), the dilution test tube (3) for experiments, the sample tube (6) for experiments, the cleaning block (7), the connecting rod (4) and the needle tube (5) are placed on the top shell of the housing (1); the small volume liquid transfer component, the large volume liquid transfer component and the horizontal linear reciprocating mechanism (21) are placed in the housing (1).

3. A pipetting diluter according to claim 2, characterized in that: The small volume pipetting assembly is supported and connected to the housing (1) via the first bottom plate (11); the large volume pipetting assembly is supported and connected to the housing (1) via the second bottom plate (16).

4. A pipetting diluter according to claim 3, characterized in that: The horizontal linear reciprocating mechanism (21) and the vertical linear reciprocating mechanism (22) are supported and connected to the inside of the housing (1) via a fixing plate.

5. A pipetting diluter according to claim 4, characterized in that: The top surface of the cleaning block (7) is provided with a plurality of cleaning pools (25), each of which is provided with a liquid storage pool (26) in parallel, and the top surfaces of the cleaning pools (25) and the liquid storage pools (26) are connected via an overflow groove (28); the bottom of the liquid storage pools (26) is connected to a drainage channel (27), and the drainage channel (27) is used to discharge excess liquid.

6. A pipetting diluter according to claim 5, characterized in that: The linear reciprocating mechanism comprises a No. 3 screw motor and a No. 3 guide rail. The No. 2 screw motor output shaft is connected to the No. 3 screw. The No. 3 screw is provided with a No. 3 slider. The No. 3 slider slides on the No. 3 guide rail. The No. 3 slider of the vertical linear reciprocating mechanism (22) is connected to a connecting rod (4).

7. A pipetting diluter according to claim 6, characterized in that: The No. 1 three-way valve (23) and the No. 2 three-way valve (24) are solenoid valves.

8. A pipetting diluter according to claim 7, characterized in that: The liquid transfer diluter is equipped with a dilution test tube rack and a sample tube rack.

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