PCR (Polymerase Chain Reaction) consumable for automatic nucleic acid detection

Abstract

The utility model discloses a PCR (Polymerase Chain Reaction) consumable for automatic nucleic acid detection, which comprises a storage box and a box cover, the storage box and the box cover are buckled through a lock catch, a partition plate is fixed in the storage box, a plurality of test tube grooves are formed in the partition plate, test tubes are inserted in the test tube grooves, a support plate is connected in the storage box in a sliding manner, and the support plate is positioned below the partition plate. The outer side of the periphery of the supporting plate is attached to the inner wall of the periphery of the storage box, a control assembly used for controlling the supporting plate to move up and down is arranged in the storage box, the supporting plate is used for ejecting the test tubes out of the test tube grooves when moving upwards, and a blocking assembly used for blocking the test tubes is arranged above the partition plate. When the test tube needs to be taken out, a rotating handle is rotated to drive a two-way screw rod to rotate, the two-way screw rod drives two threaded sleeves to be close to each other when rotating, so that a rotating rod is driven to rotate, a supporting plate is driven to move upwards when the rotating rod rotates, and the test tube is moved out of a test tube groove by a certain distance when the supporting plate moves upwards, so that the test tube is conveniently taken out.

Description

Technical Field

[0001] The utility model relates to the technical field of PCR reaction consumables, in particular to a PCR reaction consumable for automated nucleic acid detection. Background Art

[0002] PCR, or polymerase chain reaction, is currently the most common method for nucleic acid detection. The PCR reaction requires the nucleic acid sample to be mixed with different reagent components in the PCR reaction consumables, and the PCR reaction consumables carry the reaction solution through several temperature cycles to complete the amplification and detection of the nucleic acid.

[0003] Publication number CN 216303822 U discloses a PCR reaction consumable for automated nucleic acid detection. The cover of the utility model is fixedly installed with the box body by pressing the pressure plate, and the disassembly is also convenient. It changes the traditional box body buckling method of squeezing and deforming the plastic, makes the lock less likely to be damaged, and increases the service life.

[0004] During use of the above-mentioned prior art, after the test tube is placed in the test tube slot, the test tube is supported by the connecting plate so that it will not fall into the test tube slot. However, the thickness of the connecting plate is usually thin, which means that when the test tube needs to be taken out of the test tube slot later, the connecting plate needs to be manually removed. The connecting plate is thin, and multiple test tubes are filled with reaction liquid, which makes it more difficult to take out the connecting plate. Moreover, the above-mentioned prior art only seals the test tube by a cover plate, and its sealing performance is poor. Shaking of the storage box during transfer can easily cause the reaction liquid in the test tube to overflow. Utility Model Content

[0005] (I) Purpose of the utility model

[0006] In view of this, the purpose of the utility model is to propose a PCR reaction consumables for automated nucleic acid detection. The technical problem to be solved is that when the test tube needs to be taken out from the test tube slot, it is necessary to manually buckle the connecting plate, and the connecting plate is relatively thin. In addition, multiple test tubes are filled with reaction liquid, which makes it more difficult to take out the connecting plate. In addition, the existing technology only seals the test tube with a cover plate, and its sealing performance is poor. The shaking of the storage box during transfer can easily cause the reaction liquid in the test tube to overflow.

[0007] (II) Technical solution

[0008] In order to achieve the above technical objectives, the utility model provides a PCR reaction consumable for automated nucleic acid detection:

[0009] It includes a storage box and a box cover, and the storage box and the box cover are buckled by a lock, a partition is fixed in the storage box, a plurality of test tube slots are opened in the partition, test tubes are inserted in the test tube slots, a support plate is slidably connected in the storage box, and the support plate is located below the partition, the outer sides of the support plate are all in contact with the inner walls of the storage box, a control component for controlling the up and down movement of the support plate is arranged in the storage box, and the support plate is used to push the test tube out of the test tube slot when it moves up, and a blocking component for blocking the test tube is arranged above the partition.

[0010] Preferably, the control component includes a bidirectional screw rotatably connected inside the storage box, a turning handle is fixed to the end of the bidirectional screw extending outside the storage box, two threaded sleeves are threadedly connected to the bidirectional screw, a turning rod is hinged on the threaded sleeve, and one end of the turning rod away from the threaded sleeve is hinged to the bottom of the support plate.

[0011] Preferably, the two thread sections on the bidirectional screw have opposite directions, and the two thread sleeves are respectively located on different thread sections of the bidirectional screw.

[0012] Preferably, the plugging assembly includes a plurality of pressure plates, and a plurality of sealing plugs are fixed to the bottom of the pressure plates.

[0013] Preferably, a circular hole is provided in the middle of the pressing plate, and two notches connected to the circular hole are also provided on the pressing plate.

[0014] Preferably, a plurality of cylinders are fixed on the partition, a disc is rotatably connected to the top of the cylinder, and the diameter of the disc is smaller than the diameter of the circular hole, and the disc can pass through the circular hole, and two baffles are fixed on the outside of the disc, and the baffles can pass through the slot.

[0015] Preferably, an annular plate is slidably connected to the outer side of the cylinder, and the diameter of the annular plate is larger than the diameter of the circular hole. A spring is sleeved on the outer side of the cylinder, and one end of the spring abuts against the partition plate, and the other end of the spring abuts against the annular plate.

[0016] It can be seen from the above technical solutions that the present application has the following beneficial effects:

[0017] 1: When the test tube needs to be taken out, the handle is turned to drive the bidirectional screw to rotate. When the bidirectional screw rotates, the two threaded sleeves are driven to approach each other, thereby driving the rotating rod to rotate. When the rotating rod rotates, the support plate is driven to move up. When the support plate moves up, the test tube is moved out of the test tube slot for a distance, thereby facilitating the removal of the test tube.

[0018] 2: Move the pressure plate so that the disc and baffle pass through the circular hole and the slot respectively, and continue to move the pressure plate downward to squeeze the annular plate downward and compress the spring. When the disc and baffle completely pass through the circular hole and the slot respectively, the sealing plug will block the test tube. Then rotate the baffle and disc 90 degrees so that the top of the baffle and the pressure plate are against each other, that is, the pressure plate and the sealing plug are fixed, so that the test tube maintains a good sealing performance to avoid overflow of the reaction liquid. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings in the following description are only embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0020] Figure 1 A schematic diagram of the structure of a PCR reaction consumable for automated nucleic acid detection provided by the utility model;

[0021] Figure 2 A schematic diagram of a side cross-sectional structure of a PCR reaction consumable for automated nucleic acid detection provided by the utility model;

[0022] Figure 3 A schematic diagram of the partial structure of a PCR reaction consumable for automated nucleic acid detection provided by the utility model;

[0023] Figure 4 A bottom view structural diagram of the pressing plate and the sealing plug provided by the utility model;

[0024] Figure 5 A schematic diagram of the partial structure of a PCR reaction consumable for automated nucleic acid detection provided by the utility model;

[0025] Figure 6 The utility model provides Figure 5 Schematic diagram of the structure at A in the middle.

[0026] Description of the drawings: 1. Storage box; 2. Box cover; 3. Lock; 4. Partition; 5. Test tube slot; 6. Test tube; 7. Support plate; 8. Bidirectional screw; 9. Threaded sleeve; 10. Turn rod; 11. Turn handle; 12. Press plate; 13. Sealing plug; 14. Round hole; 15. Notch; 16. Disc; 17. Baffle; 18. Cylinder; 19. Ring plate; 20. Spring. DETAILED DESCRIPTION

[0027] The following description is merely exemplary in nature and is not intended to limit the present disclosure, application, and use. It should be understood that in all of these figures, the same or similar reference numerals indicate the same or similar parts and features. The various drawings only schematically represent the concepts and principles of the embodiments of the present disclosure, and do not necessarily show the specific dimensions and proportions of the various embodiments of the present disclosure. Specific parts in specific drawings may be exaggerated to illustrate the relevant details or structures of the embodiments of the present disclosure.

[0028] Reference Figure 1-6 :

[0029] The utility model provides a PCR reaction consumable for automated nucleic acid detection, comprising a storage box 1 and a box cover 2, wherein the storage box 1 and the box cover 2 are buckled together by a lock 3, a partition 4 is fixed inside the storage box 1, a plurality of test tube slots 5 are provided inside the partition 4, test tubes 6 are inserted into the test tube slots 5, a support plate 7 is slidably connected inside the storage box 1, and the support plate 7 is located below the partition 4, the outer sides of the support plate 7 are all in contact with the inner walls of the storage box 1, a control component for controlling the up and down movement of the support plate 7 is provided inside the storage box 1, and the support plate 7 is used to push the test tube 6 out of the test tube slot 5 when moving up, and a blocking component for blocking the test tube 6 is provided above the partition 4.

[0030] Specifically, the control component includes a bidirectional screw 8 rotatably connected inside the storage box 1, a turning handle 11 is fixed to the end of the bidirectional screw 8 extending outside the storage box 1, two threaded sleeves 9 are threadedly connected to the bidirectional screw 8, and a turning rod 10 is hinged on the threaded sleeve 9, and the end of the turning rod 10 away from the threaded sleeve 9 is hinged to the bottom of the support plate 7, the two sections of the thread on the bidirectional screw 8 are in opposite directions, and the two threaded sleeves 9 are respectively located on different thread sections of the bidirectional screw 8.

[0031] It should be noted that the test tube 6 is placed in the test tube slot 5 so that the bottom of the test tube 6 and the support plate 7 are against each other. When the test tube 6 needs to be taken out later, the handle 11 is turned to drive the bidirectional screw 8 to rotate. When the bidirectional screw 8 rotates, it drives the two threaded sleeves 9 to approach each other. When the two threaded sleeves 9 approach each other, it drives the rotating rod 10 to rotate. When the rotating rod 10 rotates, it drives the support plate 7 to move upward, thereby moving the test tube 6 out of the test tube slot 5 for a distance, making it easier to take out the test tube 6.

[0032] Embodiment 2

[0033] A PCR reaction consumable for automated nucleic acid detection, based on the first embodiment, comprises a blocking component comprising a plurality of pressing plates 12, a plurality of sealing plugs 13 being fixed to the bottom of the pressing plate 12, a circular hole 14 being provided in the middle of the pressing plate 12, and two notches 15 being provided on the pressing plate 12 and being connected to the circular hole 14, a plurality of cylinders 18 being fixed on the partition 4, a circular disk 16 being rotatably connected to the top of the cylinder 18, and the diameter of the circular disk 16 being smaller than the diameter of the circular hole 14, and the circular disk 16 being able to pass through the circular hole 14, two baffles 17 being fixed to the outside of the circular disk 16, and the baffles 17 being able to pass through the notches 15, an annular plate 19 being slidably connected to the outside of the cylinder 18, and the diameter of the annular plate 19 being larger than the diameter of the circular hole 14, a spring 20 being sleeved on the outside of the cylinder 18, and one end of the spring 20 being against the partition 4, and the other end of the spring 20 being against the annular plate 19.

[0034] It should be noted that, after the test tube 6 is placed in the test tube slot 5, the baffle 17 is first rotated to be parallel to the slot 15, and then the pressure plate 12 is moved downward, so that the disc 16 passes through the circular hole 14, and the baffle 17 passes through the slot 15, and then the pressure plate 12 is continued to be moved downward so that the pressure plate 12 squeezes the annular plate 19 and drives the annular plate 19 to move downward, so that the annular plate 19 compresses the spring 20. When the disc 16 and the baffle 17 are completely penetrated from the circular hole 14 and the slot 15, the sealing plug 13 blocks the test tube 6 at this time, and then the baffle 17 and the disc 16 are rotated ninety degrees, so that the baffle 17 and the top of the pressure plate 12 are abutted, and the spring 20 is in a compressed state at this time, so that the test tube 6 maintains a better sealing performance.

[0035] The exemplary implementation scheme of the present disclosure is described in detail above with reference to the preferred embodiments. However, it can be understood by those skilled in the art that, without departing from the concept of the present disclosure, various modifications and variations can be made to the above-mentioned specific embodiments, and various technical features and structures proposed in the present disclosure can be combined in various ways without exceeding the protection scope of the present disclosure, which is determined by the attached claims.

Claims

1. A PCR reaction consumable for automated nucleic acid detection, comprising a storage box (1) and a box cover (2), wherein the storage box (1) and the box cover (2) are buckled together by a lock (3), characterized in that: A partition (4) is fixed inside the storage box (1), a plurality of test tube slots (5) are provided inside the partition (4), test tubes (6) are inserted into the test tube slots (5), a support plate (7) is slidably connected inside the storage box (1), and the support plate (7) is located below the partition (4), the outer sides of the support plate (7) are in contact with the inner walls of the storage box (1), a control component for controlling the upward and downward movement of the support plate (7) is provided inside the storage box (1), and the support plate (7) is used to push the test tube (6) out of the test tube slot (5) when moving upward, and a blocking component for blocking the test tube (6) is provided above the partition (4).

2. A PCR reaction consumable for automated nucleic acid detection according to claim 1, characterized in that: The control assembly comprises a bidirectional screw (8) rotatably connected in the storage box (1), a turning handle (11) being fixed to the end of the bidirectional screw (8) extending outside the storage box (1), two threaded sleeves (9) being threadedly connected to the bidirectional screw (8), a turning rod (10) being hinged to the threaded sleeve (9), and an end of the turning rod (10) away from the threaded sleeve (9) being hinged to the bottom of the support plate (7).

3. A PCR reaction consumable for automated nucleic acid detection according to claim 2, characterized in that: The two thread sections on the bidirectional screw (8) are in opposite directions, and the two thread sleeves (9) are respectively located on different thread sections of the bidirectional screw (8).

4. The PCR reaction consumables for automated nucleic acid detection according to claim 1, characterized in that: The plugging assembly comprises a plurality of pressure plates (12), and a plurality of sealing plugs (13) are fixed to the bottom of the pressure plates (12).

5. A PCR reaction consumable for automated nucleic acid detection according to claim 4, characterized in that: A circular hole (14) is provided in the middle of the pressing plate (12), and two notches (15) connected to the circular hole (14) are also provided on the pressing plate (12).

6. A PCR reaction consumable for automated nucleic acid detection according to claim 5, characterized in that: A plurality of cylinders (18) are fixed on the partition (4); a disc (16) is rotatably connected to the top of the cylinder (18); the diameter of the disc (16) is smaller than the diameter of the circular hole (14); and the disc (16) can pass through the circular hole (14); two baffles (17) are fixed on the outer side of the disc (16); and the baffles (17) can pass through the notch (15).

7. A PCR reaction consumable for automated nucleic acid detection according to claim 6, characterized in that: An annular plate (19) is slidably connected to the outside of the cylinder (18), and the diameter of the annular plate (19) is greater than the diameter of the circular hole (14). A spring (20) is sleeved on the outside of the cylinder (18), and one end of the spring (20) abuts against the partition (4), and the other end of the spring (20) abuts against the annular plate (19).

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