Marking device for fully fusing sample and alkaline phosphatase antibody

By designing a sample and alkaline phosphatase antibody labeling device containing fusion, auxiliary, cleaning and collection mechanisms, the problems of phosphate buffer waste and impurity residue during the labeling process are solved, and an efficient and accurate labeling process is achieved.

CN222618637UActive Publication Date: 2025-03-14HANGZHOU FUSHENG BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202420680129.0
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-04-03
Publication Date
2025-03-14
Estimated Expiration
2034-04-03

AI Technical Summary

Technical Problem

Existing samples and alkaline phosphatase antibody labeling devices require multiple dialysis during the labeling process, resulting in wasting of phosphate buffer, and residual impurities are easily attached to the container after labeling, affecting subsequent use.

Method used

A fully fused sample and alkaline phosphatase antibody labeling device is designed, including a labeling cylinder, a fusion mechanism, an auxiliary mechanism, a cleaning mechanism and a collection mechanism. The fusion mechanism promotes the mixing of samples and antibodies through a hollow motor and spray head, the auxiliary mechanism is used for quantitative dialysis and cleaning, and the cleaning mechanism and the collection mechanism are used for cleaning and collecting phosphate buffer.

Benefits of technology

It effectively reduces the waste of phosphate buffer, cleanses the residual impurities in the labeling device, and improves the efficiency and accuracy of the labeling of samples and alkaline phosphatase antibodies.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model discloses a marking device for fully fusing a sample and an alkaline phosphatase antibody, which comprises a marking cylinder, a fusing mechanism arranged above the marking cylinder, an auxiliary mechanism connected to one end of the fusing mechanism, a cleaning mechanism mounted at one end of the auxiliary mechanism, and a collecting mechanism arranged on one side of the auxiliary mechanism. The auxiliary mechanism is arranged, so that auxiliary cleaning of residual impurities attached to the inner wall part of a container of the sample and alkaline phosphatase antibody labeling device is facilitated, and subsequent use of the sample and alkaline phosphatase antibody labeling is facilitated; a phosphate buffer and a cleaning solution are conveniently collected when a sample and an alkaline phosphatase antibody are subjected to phosphate buffer dialysis or cleaning, the waste of the phosphate buffer and the cleaning solution is favorably reduced, and the fusion mechanism is arranged on the marking cylinder, so that the sample and the alkaline phosphatase antibody are conveniently subjected to auxiliary fusion stirring to promote reaction; the labeling of the sample and the alkaline phosphatase antibody is facilitated.
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Description

Technical Field

[0001] The utility model relates to a field, in particular to a fully integrated sample and alkaline phosphatase antibody labeling device. Background Art

[0002] The main function of the fully integrated sample and alkaline phosphatase antibody labeling device is to promote the full integration between the sample and the alkaline phosphatase antibody so that the target molecule can be accurately identified and analyzed in subsequent detection or experiments, and it is necessary to ensure uniform mixing between the sample and the alkaline phosphatase antibody labeling in order to achieve an efficient and accurate labeling reaction.

[0003] However, the sample and alkaline phosphatase antibody labeling device needs to use phosphate buffer for dialysis treatment multiple times during labeling, and the phosphate buffer used for multiple dialysis cannot be collected, which makes the phosphate buffer easy to be wasted. At the same time, after the sample and alkaline phosphatase antibody labeling device is labeled, residual impurities are easy to adhere to the inside of the container used for sample and alkaline phosphatase antibody labeling, which is easy to affect the subsequent sample and alkaline phosphatase antibody labeling.

[0004] Currently, no effective solution has been proposed for the problems in the related technologies. Utility Model Content

[0005] 1. Technical issues to be resolved

[0006] In view of the deficiencies in the prior art, the utility model provides a device for labeling a sample and an alkaline phosphatase antibody, which has the advantages of the above-mentioned technical problems and solves the problems in the prior art.

[0007] (II) Technical solution

[0008] In order to achieve the advantages of the above technical problems, the specific technical solutions adopted by the utility model are as follows:

[0009] A fully fused sample and alkaline phosphatase antibody labeling device, comprising a labeling tube, a fusion mechanism is arranged above the labeling tube, and an auxiliary mechanism is connected to one end of the fusion mechanism;

[0010] A cleaning mechanism is installed at one end of the auxiliary mechanism, and a collecting mechanism is arranged at one side of the auxiliary mechanism.

[0011] Furthermore, the fusion mechanism includes a hollow motor fixedly arranged above the marking cylinder, a hollow rod is installed at the output end of the hollow motor, one end of the hollow rod passes through the top of the marking cylinder, a fusion rod is arranged on the surface of the hollow rod, and spray heads distributed equidistantly are arranged on the surface of the fusion rod.

[0012] Furthermore, the auxiliary mechanism includes an auxiliary tube connected to the output end of the hollow motor through a bearing, a delivery pump 1 is installed at one end of the auxiliary tube, the output end of the delivery pump 1 is penetrated by an auxiliary box through a connecting tube 1, and one side of the auxiliary box is fixedly connected to the outside of the marking tube.

[0013] Furthermore, the cleaning mechanism includes a delivery pump 2 installed at one end of the auxiliary pipe, the delivery end of the delivery pump 2 is penetrated by a cleaning box through a connecting pipe 2, and one side of the cleaning box is fixedly connected to the marking tube.

[0014] Furthermore, the collecting mechanism includes a delivery pipe arranged on the cleaning box and the auxiliary box, one end of the delivery pipe is located at the inner wall of the auxiliary box and the cleaning box and a delivery pump three is installed, the other end of the delivery pipe is installed with a filter box, the inner wall of the filter box is installed with an activated carbon plate and a polyester fiber plate, wherein a filter seat is connected to the bottom of the filter box through a connecting pipe three, the filter seat is penetrated by the marking cylinder, and another filter box is penetrated by the marking cylinder through a connecting pipe four.

[0015] Furthermore, a placing bucket is passed through the marking cylinder, the auxiliary box and the cleaning box, and a sealing cover is installed on the inner wall of the placing bucket.

[0016] Furthermore, an output pipe runs through the bottom of the marking tube, and a control valve is installed at one end of the output pipe.

[0017] Furthermore, a plurality of support columns are fixedly arranged below the marking tube, and protective pads are installed below the support columns.

[0018] (III) Beneficial effects

[0019] Compared with the prior art, the utility model has the following beneficial effects:

[0020] (1) The cleaning mechanism provided in the auxiliary mechanism facilitates auxiliary cleaning of residual impurities attached to the inside of the sample and alkaline phosphatase antibody labeling device container, which is beneficial to the subsequent use of the sample and alkaline phosphatase antibody labeling. At the same time, the collection mechanism provided in the auxiliary mechanism facilitates the collection of phosphate buffer and cleaning solution when the sample and alkaline phosphatase antibody are dialyzed or cleaned with phosphate buffer, which is beneficial to reducing the waste of phosphate buffer and cleaning.

[0021] (2) The fusion mechanism provided in the marking cylinder facilitates the auxiliary fusion and stirring of the sample and the alkaline phosphatase antibody to promote the reaction, which is beneficial to the labeling of the sample and the alkaline phosphatase antibody and improves the labeling efficiency of the sample and the alkaline phosphatase antibody. At the same time, the auxiliary mechanism provided in the fusion mechanism facilitates the quantitative dialysis treatment of the sample and the alkaline phosphatase antibody, which is beneficial to the auxiliary removal of the substances produced by the fusion of the sample and the alkaline phosphatase antibody. At the same time, the placement bucket and the sealing cover provided in the marking cylinder, the auxiliary box and the cleaning box facilitate the placement and replenishment of the sample, the alkaline phosphatase antibody, the phosphate buffer solution and the cleaning solution, which is beneficial to the labeling and subsequent use. At the same time, the output pipe and the control valve provided in the marking cylinder facilitate the discharge of the labeled sample and the alkaline phosphatase antibody, which is beneficial to the subsequent in-depth detection by the staff. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0023] Figure 1 This is a schematic diagram of a fully integrated sample and alkaline phosphatase antibody labeling device according to an embodiment of the utility model. Figure 1 ;

[0024] Figure 2 This is a schematic diagram of a fully integrated sample and alkaline phosphatase antibody labeling device according to an embodiment of the utility model. Figure 2 ;

[0025] Figure 3 It is a schematic diagram of the internal structure of a device for fully integrating a sample and an alkaline phosphatase antibody labeling according to an embodiment of the utility model;

[0026] Figure 4 It is a partial structural diagram of an auxiliary mechanism and a collecting mechanism of a device for fully integrating a sample and an alkaline phosphatase antibody labeling according to an embodiment of the utility model;

[0027] Figure 5 It is a partial structural schematic diagram of a cleaning mechanism and a collecting mechanism of a device for fully integrating a sample and an alkaline phosphatase antibody labeling according to an embodiment of the utility model;

[0028] Figure 6 It is a schematic diagram of the fusion mechanism structure for fully fusing a sample with an alkaline phosphatase antibody labeling device according to an embodiment of the utility model.

[0029] In the figure:

[0030] 1. Marking cylinder; 2. Fusion mechanism; 201. Hollow motor; 202. Hollow rod; 203. Fusion rod; 204. Spray head; 3. Auxiliary mechanism; 301. Auxiliary pipe; 302. Delivery pump 1; 303. Auxiliary box; 4. Cleaning mechanism; 401. Delivery pump 2; 402. Cleaning box; 5. Collecting mechanism; 501. Delivery pipe; 502. Delivery pump 3; 503. Filter box; 504. Activated carbon plate; 505. Polyester fiber plate; 506. Filter seat; 6. Placement bucket; 7. Sealing cover; 8. Output pipe; 9. Control valve; 10. Support column; 11. Protective pad. DETAILED DESCRIPTION

[0031] To further illustrate the technical solution of the present application, the present invention provides drawings, which are part of the disclosure of the present invention and are mainly used to illustrate the implementation methods. They can be used in conjunction with the relevant descriptions in the specification to explain the operating principles of the implementation methods. With reference to these contents, ordinary technicians in this field should be able to understand other possible implementation methods and advantages of the present invention. The components in the figures are not drawn to scale, and similar component symbols are generally used to represent similar components.

[0032] like Figure 1 - Figure 4 , Figure 6 As shown, the utility model provides the following technical solutions: a fully fused sample and alkaline phosphatase antibody labeling device, comprising a labeling cylinder 1, which is used to store the sample and the alkaline phosphatase antibody for fusion labeling, an output tube 8 is passed through the bottom of the labeling cylinder 1, which is used to discharge the labeled sample and the alkaline phosphatase antibody, and a control valve 9 is installed at one end of the output tube 8, and a plurality of support columns 10 are fixedly arranged under the labeling cylinder 1 to improve the stability of the labeling cylinder 1, and the number of the support columns 10 is three, and a protective pad 11 is installed under the support columns 10;

[0033] A fusion mechanism 2 is arranged above the marking cylinder 1. The fusion mechanism 2 includes a hollow motor 201 fixedly arranged above the marking cylinder 1. A hollow rod 202 is installed at the output end of the hollow motor 201. One end of the hollow rod 202 penetrates the top of the marking cylinder 1. A fusion rod 203 is arranged on the surface of the hollow rod 202, which is used to mix and stir the sample with the alkaline phosphatase antibody to promote the reaction, which is conducive to better labeling the sample and the alkaline phosphatase antibody. The interior of the fusion rod 203 and the hollow rod 202 is a hollow structure. The surface of the fusion rod 203 is provided with spray heads 204 distributed at equal distances, which can not only clean the marking cylinder 1, but also dialyze the sample and the alkaline phosphatase antibody to ensure that unnecessary substances are completely removed;

[0034] One end of the fusion mechanism 2 is connected to an auxiliary mechanism 3, which includes an auxiliary tube 301 connected to the output end of the hollow motor 201 through a bearing, and is used to transport cleaning liquid and phosphate buffer solution. One end of the auxiliary tube 301 is equipped with a delivery pump 302, and the output end of the delivery pump 302 is penetrated by an auxiliary box 303 through a connecting tube, and is used to store phosphate buffer solution. One side of the auxiliary box 303 is fixedly connected to the outside of the marking tube 1.

[0035] Specifically, by providing the fusion mechanism 2 in the marking tube 1, it is convenient to assist in the fusion and stirring of the sample and the alkaline phosphatase antibody to promote the reaction, which is beneficial to the labeling of the sample and the alkaline phosphatase antibody. At the same time, the auxiliary mechanism 3 provided in the fusion mechanism 2 is convenient for the quantitative dialysis treatment of the sample and the alkaline phosphatase antibody, which is beneficial to the auxiliary removal of the substance produced by the fusion of the sample and the alkaline phosphatase antibody. At the same time, the output pipe 8 and the control valve 9 provided in the marking tube 1 are convenient for the discharge of the labeled sample and the alkaline phosphatase antibody. At the same time, the support column 10 and the protective pad 11 provided in the marking tube 1 are beneficial to improving the stability of the marking tube 1 when labeling the sample and the alkaline phosphatase antibody.

[0036] like Figure 1 - Figure 3 , Figure 5 As shown, a cleaning mechanism 4 is installed at one end of the auxiliary mechanism 3, and the cleaning mechanism 4 includes a delivery pump 2 401 installed at one end of the auxiliary tube 301, and a cleaning box 402 is penetrated through the delivery end of the delivery pump 2 401 through a connecting tube 2, which is used to store cleaning liquid, and one side of the cleaning box 402 is fixedly connected to the marking cylinder 1, and a placement bucket 6 is penetrated above the marking cylinder 1 and the auxiliary box 303 and the cleaning box 402, which is used to place the sample to be fused and the alkaline phosphatase antibody, the cleaning liquid, and the phosphate buffer solution, and a sealing cover 7 is installed on the inner wall of the placement bucket 6 for shielding;

[0037] A collecting mechanism 5 is arranged on one side of the auxiliary mechanism 3, and the collecting mechanism 5 includes a cleaning box 402 and a delivery pipe 501 arranged on the auxiliary box 303, one end of the delivery pipe 501 is located in the auxiliary box 303, a delivery pump 3 502 is installed on the inner wall of the cleaning box 402, a filter box 503 is installed on the other end of the delivery pipe 501, an activated carbon plate 504 and a polyester fiber plate 505 are installed on the inner wall of the filter box 503, wherein a filter seat 506 is connected to the bottom of the filter box 503 through a connecting pipe 3, which is used to shield the sample and the alkaline phosphatase antibody when collecting the phosphate buffer, the filter seat 506 is penetrated with the marking cylinder 1, and the bottom of another filter box 503 is penetrated with the marking cylinder 1 through a connecting pipe 4;

[0038] The delivery pump three 502, the delivery pump two 401, the hollow motor 201, and the control valve 9 are electrically connected to a controller during actual use, and the controller, the delivery pump three 502, the delivery pump two 401, the hollow motor 201, and the control valve 9 are electrically connected to an external power supply.

[0039] Specifically, the cleaning mechanism 4 provided in the auxiliary mechanism 3 facilitates auxiliary cleaning of residual impurities attached to the inside of the sample and alkaline phosphatase antibody labeling device container. At the same time, the collecting mechanism 5 provided in the auxiliary mechanism 3 facilitates the collection of phosphate buffer and cleaning solution during phosphate buffer dialysis or cleaning of the sample and alkaline phosphatase antibody, which is beneficial to reducing the waste of phosphate buffer and cleaning solution. At the same time, the placement bucket 6 and sealing cover 7 provided in the marking cylinder 1, the auxiliary box 303 and the cleaning box 402 facilitate the placement and replenishment of the sample, alkaline phosphatase antibody, phosphate buffer and cleaning solution, which is beneficial to labeling and subsequent use.

[0040] In summary, with the aid of the above-mentioned technical scheme of the utility model, when in use, the staff will place the marking tube 1 to the position where it needs to be placed, and then pass through the placement bucket 6 and the sealing cover 7 provided on the marking tube 1, so as to facilitate the placement of the sample and the alkaline phosphatase antibody, which is beneficial to the labeling of the sample and the alkaline phosphatase antibody. After placement, the fusion mechanism 2 provided on the marking tube 1 is convenient for auxiliary fusion and stirring of the sample and the alkaline phosphatase antibody to promote the reaction, which is beneficial to the labeling of the sample and the alkaline phosphatase antibody. During fusion, the auxiliary mechanism 3 provided on the fusion mechanism 2 is convenient for quantitatively transporting the phosphate buffer to the hollow motor 201, the hollow rod 202, the fusion rod 203, and the spray head 204 to spray the sample and the alkaline phosphatase antibody fused inside the marking tube 1, which is beneficial to quantitative dialysis of the sample and the alkaline phosphatase antibody. The treatment is beneficial to assist in removing the substance produced by the fusion of the sample and the alkaline phosphatase antibody. The phosphate buffer after the removal of the substance passes through the collecting mechanism 5 set in the auxiliary mechanism 3, which is convenient for collecting the phosphate buffer for the sample and the alkaline phosphatase antibody, which is beneficial to reducing the waste of the phosphate buffer. When cleaning after marking, it passes through the cleaning mechanism 4 set in the auxiliary mechanism 3, which is convenient for assisting in cleaning the residual impurities attached to the inside of the marking tube 1. The cleaning liquid after cleaning passes through the cleaning liquid when cleaning the inside of the marking tube 1, which is convenient for collecting the cleaning liquid, which is beneficial to reducing the waste of the cleaning liquid. When the phosphate buffer and the cleaning liquid need to be supplemented, they pass through the placement bucket 6 and the sealing cover 7 set in the auxiliary box 303 and the cleaning box 402, which are convenient for placing and replenishing the phosphate buffer and the cleaning liquid, which is beneficial to subsequent use.

[0041] In the present utility model, unless otherwise clearly stipulated and limited, the terms such as "installation", "setting", "connection", "fixation" and "screw-on" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integrated connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. Unless otherwise clearly defined, ordinary technicians in this field can understand the specific meanings of the above terms in the utility model according to the specific circumstances.

[0042] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A fully integrated sample and alkaline phosphatase antibody labeling device, characterized in that: The invention comprises a marking cylinder (1), a fusion mechanism (2) is arranged above the marking cylinder (1), the fusion mechanism (2) comprises a hollow motor (201) fixedly arranged above the marking cylinder (1), a hollow rod (202) is installed at the output end of the hollow motor (201), one end of the hollow rod (202) penetrates the top of the marking cylinder (1), a fusion rod (203) is arranged on the surface of the hollow rod (202), and spray heads (204) distributed at equal distances are arranged on the surface of the fusion rod (203); One end of the fusion mechanism (2) is connected to an auxiliary mechanism (3), and the auxiliary mechanism (3) comprises an auxiliary tube (301) connected to the output end of the hollow motor (201) via a bearing, and one end of the auxiliary tube (301) is installed with a delivery pump (302), and the output end of the delivery pump (302) is penetrated by an auxiliary box (303) via a connecting tube, and one side of the auxiliary box (303) is fixedly connected to the outside of the marking tube (1); A cleaning mechanism (4) is installed at one end of the auxiliary mechanism (3), and the cleaning mechanism (4) comprises a second delivery pump (401) installed at one end of the auxiliary pipe (301), and a cleaning box (402) is passed through the delivery end of the second delivery pump (401) via a second connecting pipe, and one side of the cleaning box (402) is fixedly connected to the marking tube (1); A collecting mechanism (5) is provided on one side of the auxiliary mechanism (3), and the collecting mechanism (5) comprises a cleaning box (402) and a delivery pipe (501) provided on the auxiliary box (303). One end of the delivery pipe (501) is located on the inner wall of the auxiliary box (303) and the cleaning box (402) and a delivery pump (502) is installed. A filter box (503) is installed on the other end of the delivery pipe (501). An activated carbon plate (504) and a polyester fiber plate (505) are installed on the inner wall of the filter box (503). A filter seat (506) is connected to the bottom of the filter box (503) through a connecting pipe (3), and the filter seat (506) and the marking cylinder (1) are penetrated. Another filter box (503) is penetrated to the bottom of the marking cylinder (1) through a connecting pipe (4).

2. A fully integrated sample and alkaline phosphatase antibody labeling device according to claim 1, characterized in that: A placement bucket (6) is passed through the marking cylinder (1), the auxiliary box (303) and the cleaning box (402), and a sealing cover (7) is installed on the inner wall of the placement bucket (6).

3. A fully integrated sample and alkaline phosphatase antibody labeling device according to claim 1, characterized in that: An output pipe (8) passes through the bottom of the marking tube (1), and a control valve (9) is installed at one end of the output pipe (8).

4. A fully integrated sample and alkaline phosphatase antibody labeling device according to claim 1, characterized in that: A plurality of support columns (10) are fixedly arranged below the marking cylinder (1), and a protective pad (11) is installed below the support columns (10).