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Stem cells derived from basal portion of chorionic trophoblast layer and cell therapy comprising same

a chorionic trophoblast layer and stem cell technology, applied in the direction of skeletal/connective tissue cells, artificial cell constructs, drug compositions, etc., can solve the problems of insufficient commercialization, and insufficient stem cell research, etc., to achieve excellent tissue regeneration effect, uniform growth characteristic, and superb proliferation and differentiation characteristics

Active Publication Date: 2019-03-19
SAMSUNG LIFE PUBLIC WELFARE FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0013]According to the present invention, the stem cells derived from the basal portion of the chorionic trophoblast layer can exhibit uniform growth characteristic, and superb proliferation and differentiation characteristic as compared with the conventional stem cells derived from the whole placenta or other tissues, and can exhibit an excellent tissue regeneration effect in a tissue defect animal model, and thus can be usefully used as a cellular therapeutic agent.

Problems solved by technology

For this disease treatment of the human, organ transplantation, gene therapy, and the like have been proposed before, but efficient commercialization is not sufficient due to immune rejection, supplied organs shortage, and lack of knowledge about vector development or disease genes.
Since the embryonic stem cells have ethical issue, there is a limitation to be used as a cellular therapeutic agent, however the adult stem cells can be mainly extracted from fat, umbilical cord blood, bone marrow, placenta, and the like and have no ethical problem.
However, until now, researches on stem cells derived from a basal portion of chorionic trophoblast layer which is a part of the tissues of the placenta are not yet conducted.

Method used

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  • Stem cells derived from basal portion of chorionic trophoblast layer and cell therapy comprising same
  • Stem cells derived from basal portion of chorionic trophoblast layer and cell therapy comprising same
  • Stem cells derived from basal portion of chorionic trophoblast layer and cell therapy comprising same

Examples

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Effect test

example 1

of Stem Cells Derived from Basal Portion of Chorionic Trophoblast Layer which is a Part of the Tissues of Placenta

[0064]The placenta was collected from the mother agreeing on donation in a normal cesarean delivery at the Samsung Seoul Hospital according to a guideline for clinical test ethics commission of the Samsung Seoul Hospital. The collected placenta was put in a sterile container and then transferred, an amniotic membrane was removed from the transferred placenta tissue, and then a basal portion of chorionic trophoblast layer tissue which corresponds to a thickness of about 25% of an adjacent (close) portion to the chorionic membrane of the total chorionic trophoblast layer (tCT) positioned between a chorionic membrane (CM) and decidua (DC) was carefully isolated by using sterilized forcep W and knife. The isolated basal portion of chorionic trophoblast layer tissue was transferred on 150 mm dish and washed 8 to 10 times by using PBS to remove blood and blood cells. The washe...

example 2

e of Stem Cells Derived from Basal Portion of Chorionic Trophoblast Layer which is a Part of the Tissues of Placenta

[0070]The stem cells derived from the basal portion of chorionic trophoblast layer which was a part of the tissues of the placenta obtained in Example 1 were washed with PBS and cultured by replacing a DMEM medium including fetal bovine serum and antibiotics without a growth factor every 2 to 3 days. When the stem cells were grown 80% or more, the stem cells was treated with TryPLE to be isolated from the culture container, and the isolated stem cells were diluted in a ratio of 1 / 4 and then cultured in another culture container to perform a subculture. While repetitively performing the subculture, the passage number which was not sub-cultured at all was measured, and cell forms before the subculture (P0) and after long-term subculture were observed with a microscope. Further, using the stem cells derived from the whole placenta (Pla) obtained in Comparative Example 1, ...

example 3

of Colony Formation Capacity of Stem Cells Derived from Basal Portion of Chorionic Trophoblast Layer which is a Part of the Tissues of Placenta

[0073]A population doubling time and a colony formation capacity of the stem cells derived from the basal portion of chorionic trophoblast layer as a part of the tissues of the placenta obtained in Example 1 were verified. More particularly, in the stem cells derived from the basal portion of chorionic trophoblast layer obtained in Example 1, the first subculture was performed by the method of Example 2, and the stem cells were seeded by 5×103 in a dish of 100 mm at the time when the subculture was completed and then cultured in a DMEM medium including fetal bovine serum and antibiotics without a growth factor for 10 days. A time (population doubling time) taken to double the number of stem cells from P2 to P6 was measured and the number of colonies formed in the stem cells was counted by performing a Giemsa stain method in the cultured stem ...

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Abstract

The present disclosure relates to stem cells derived from the basal portion of the chorionic trophoblast layer (bCT), which is a part of the issues of the placenta, and cell therapy comprising same. Stem cells derived from the basal portion of the chorionic trophoblast layer according to the present invention exhibit uniform growth characteristic, and superb proliferation and differentiation characteristic as compared with the conventional stem cells derived from the full placenta or other tissues, and exhibit excellent tissue regeneration effect in an animal model, thus can be effectively used in cell therapy.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application is a U.S. National Stage Application under 35 U.S.C. § 371 of International Application No. PCT / KR2015 / 000205, filed Jan. 8, 2015, which claims the benefit of and priority to Korean Patent Application No. 10-2014-0002316, filed Jan. 8, 2014. Each of these applications is hereby incorporated by reference in its entirety.TECHNICAL FIELD[0002]The present disclosure provides stem cells derived from a basal portion of chorionic trophoblast layer (bCT) which is a part of the tissues of the placenta, and a cell therapy including the same.BACKGROUND ART[0003]Recently, biotechnology has proposed the possibility of new solutions to the food, environmental, and health problems as a final goal of human welfare, and among them, a technique using stem cells is emerging as a new technique of incurable disease treatment. For this disease treatment of the human, organ transplantation, gene therapy, and the like have been proposed before, ...

Claims

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Application Information

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Patent Type & Authority Patents(United States)
IPC IPC(8): A61K35/50C12N5/071C12N5/073A61K35/28A61K35/51C12N5/074A61K35/12
CPCA61K35/50A61K35/28A61K35/51C12N5/0607C12N5/0605C12N2506/025A61K2035/124A61P19/00A61P19/02A61P19/04A61P19/08A61P29/00C12N5/0619C12N5/0653C12N5/0655C12N5/066
Inventor HA, CHUL WONKIM, JIN A.
Owner SAMSUNG LIFE PUBLIC WELFARE FOUND
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